CA2021658A1 - Multiplex immunoassay system - Google Patents
Multiplex immunoassay systemInfo
- Publication number
- CA2021658A1 CA2021658A1 CA 2021658 CA2021658A CA2021658A1 CA 2021658 A1 CA2021658 A1 CA 2021658A1 CA 2021658 CA2021658 CA 2021658 CA 2021658 A CA2021658 A CA 2021658A CA 2021658 A1 CA2021658 A1 CA 2021658A1
- Authority
- CA
- Canada
- Prior art keywords
- ligands
- analyte
- specific binding
- sample
- ligand
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
Abstract
Disclosed is a method of an apparatus for assaying, in assay apparatus, for an analyte in a sample by specifically binding the analyte to a ligand having a detectable tag to form a complex, the presence of which can be determined quantitatively by measuring the amount of tag. In one embodiment, the sample is contacted in that apparatus with a test body of ligands so as to effect the specific binding. The body has at least two differently tagged different ligands in a predetermined ratio, at least a first of which tagged ligands being that which will specifically bind to the analyte. The second tagged ligand is one which will not specifically bind to the analyte or to other moieties in the sample. The relative non-specific binding characteristics of those ligands with respect to said apparatus are in a known or predetermined relationship. Quantitative measurement of those tags coupled to bound quantities of the ligands is then made substantially simultaneously. From the known proportion of ligands in the test body, the known ratio between the non-specific binding characteristics of the ligands to the apparatus, and the quantitative measurement of the bound tags, one can relatively accurately determine the specific binding of the first ligand to the analyte.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US398,621 | 1982-07-15 | ||
US39862189A | 1989-08-25 | 1989-08-25 |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2021658A1 true CA2021658A1 (en) | 1991-02-26 |
CA2021658C CA2021658C (en) | 2001-10-09 |
Family
ID=23576097
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA 2021658 Expired - Fee Related CA2021658C (en) | 1989-08-25 | 1990-07-20 | Multiplex immunoassay system |
Country Status (5)
Country | Link |
---|---|
JP (1) | JP2584530B2 (en) |
CA (1) | CA2021658C (en) |
DE (1) | DE4024350C2 (en) |
FR (1) | FR2651327B1 (en) |
GB (1) | GB2235292B (en) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU4047893A (en) * | 1992-04-06 | 1993-11-08 | Abbott Laboratories | Method and device for detection of nucleic acid or analyte using total internal reflectance |
WO2002048690A1 (en) * | 2000-12-11 | 2002-06-20 | Varian Australia Pty Ltd | Method and apparatus for analysis of multiplexed samples |
JP3429282B2 (en) | 2001-02-02 | 2003-07-22 | リサーチ・インターナショナル・インコーポレーテッド | Automated system and sample analysis method |
US20050221363A1 (en) * | 2004-04-01 | 2005-10-06 | Rules-Based Medicine, Inc. | Universal shotgun assay |
US7496245B2 (en) | 2004-08-20 | 2009-02-24 | Research International, Inc. | Misalignment compensating optical sensor and method |
US7842465B2 (en) * | 2006-01-17 | 2010-11-30 | Palo Alto Research Center Incorporated | Immunocytostaining methods for enhanced dye ratio discrimination in rare event detection |
US7651869B2 (en) | 2006-03-14 | 2010-01-26 | Research International, Inc. | Optical assay apparatus and methods |
WO2008106021A1 (en) * | 2007-02-26 | 2008-09-04 | Response Biomedical Corporation | Comparative multiple analyte assay |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3604927A (en) * | 1966-11-16 | 1971-09-14 | Block Engineering | Total reflection fluorescence spectroscopy |
US3652761A (en) * | 1969-09-04 | 1972-03-28 | Corning Glass Works | Immunochemical composites and antigen or antibody purification therewith |
DE2953610C1 (en) * | 1979-03-19 | 1985-04-11 | International Diagnostic Technology, Inc., Santa Clara, Calif. | Method for determining an immunologically active substance |
JPS5745454A (en) * | 1980-09-02 | 1982-03-15 | Fuji Photo Film Co Ltd | Immunochemical measuring method for various minor components |
JPS585659A (en) * | 1981-06-24 | 1983-01-13 | ハイブリテツク インコ−ポレ−テツド | Immunological inhibition test using single clone property antigen |
US4582809A (en) * | 1982-06-14 | 1986-04-15 | Myron J. Block | Apparatus including optical fiber for fluorescence immunoassay |
US4471058A (en) * | 1982-07-26 | 1984-09-11 | Board Of Trustees Operating Michigan State University | Method for the detection and/or determination of a polyvalent antigen using at least two different monoclonal antibodies |
US4447546A (en) * | 1982-08-23 | 1984-05-08 | Myron J. Block | Fluorescent immunoassay employing optical fiber in capillary tube |
US4584277A (en) * | 1983-04-05 | 1986-04-22 | Syntex (U.S.A.) Inc. | Fluorescent multiparameter particle analysis |
US4558014A (en) * | 1983-06-13 | 1985-12-10 | Myron J. Block | Assay apparatus and methods |
US4582791A (en) * | 1983-10-07 | 1986-04-15 | Syntex (U.S.A.) Inc. | Reducing non-specific background in immunofluorescence techniques |
US4595661A (en) * | 1983-11-18 | 1986-06-17 | Beckman Instruments, Inc. | Immunoassays and kits for use therein which include low affinity antibodies for reducing the hook effect |
JPH0789116B2 (en) * | 1985-06-14 | 1995-09-27 | 帝人株式会社 | Immunological assay method using monoclonal antibody and kit therefor |
JPS6238362A (en) * | 1985-08-12 | 1987-02-19 | Chemo Sero Therapeut Res Inst | Method for measuring tsh |
AU614935B2 (en) * | 1986-08-06 | 1991-09-19 | Multilyte Limited | Determination of analyte concentration using two labelling markers |
US4704891A (en) * | 1986-08-29 | 1987-11-10 | Becton, Dickinson And Company | Method and materials for calibrating flow cytometers and other analysis instruments |
GB8623494D0 (en) * | 1986-09-30 | 1986-11-05 | Mackay C D | Assay technique |
US4954435A (en) * | 1987-01-12 | 1990-09-04 | Becton, Dickinson And Company | Indirect colorimetric detection of an analyte in a sample using ratio of light signals |
JPH0827282B2 (en) * | 1987-07-01 | 1996-03-21 | 帝人株式会社 | Immunological assay method for human protein C, assay reagent and kit thereof |
GB2233450B (en) * | 1989-06-24 | 1993-06-30 | Univ Wales Medicine | Detecting or quantifing multiple analytes with luminescent reagents |
-
1990
- 1990-07-20 CA CA 2021658 patent/CA2021658C/en not_active Expired - Fee Related
- 1990-07-24 GB GB9016162A patent/GB2235292B/en not_active Expired - Fee Related
- 1990-07-27 DE DE19904024350 patent/DE4024350C2/en not_active Expired - Fee Related
- 1990-08-17 FR FR9010440A patent/FR2651327B1/en not_active Expired - Fee Related
- 1990-08-24 JP JP2221447A patent/JP2584530B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CA2021658C (en) | 2001-10-09 |
GB2235292B (en) | 1993-09-22 |
JPH03272466A (en) | 1991-12-04 |
DE4024350C2 (en) | 2000-03-16 |
JP2584530B2 (en) | 1997-02-26 |
DE4024350A1 (en) | 1991-02-28 |
GB9016162D0 (en) | 1990-09-05 |
FR2651327B1 (en) | 1994-03-11 |
FR2651327A1 (en) | 1991-03-01 |
GB2235292A (en) | 1991-02-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US4496654A (en) | Detection of HCG with solid phase support having avidin coating | |
US6183972B1 (en) | Method for the determination of analyte concentration in a lateral flow sandwich immunoassay exhibiting high-dose hook effect | |
EP0304202B1 (en) | Determination of ambient concentrations of several analytes | |
US5807755A (en) | Determination of ambient concentrations of several analytes | |
EP0399184A3 (en) | Reagents, methods and kits for an amphetamine-class fluorescence polarization immunoassay | |
CA2007532A1 (en) | Threshold ligand-receptor assay | |
EP0188093A3 (en) | Sandwich immunoassay | |
ES8700882A1 (en) | Simultaneous calibration heterogeneous immunoassay method, apparatus and diagnostic kit. | |
JP3135067B2 (en) | Immunodiagnostic test system and its use | |
DE69416828D1 (en) | DEVICE FOR CHROMATOGRAPHIC DETERMINATION CONTAINING COMPARABLE PARTS | |
KR890012165A (en) | Ion-Capture Analysis Method and Apparatus | |
ATE185424T1 (en) | DIFFERENTIAL BINDING AFFINITIES AND RELATED DISSOCIATION TESTING METHODS | |
ATE221997T1 (en) | IMMUNOASSAYS FOR THE DETECTION OF ANTIPHOSPHOLIPIDE ANTIBODIES | |
SE9704933D0 (en) | Method utilizing a new calibrator and test kit containing the calibrator | |
ATE186599T1 (en) | METHODS FOR NON-COMPETITIVE BINDING ASSAY | |
CA2471462A1 (en) | Internal calibration system for flow-through assays | |
WO1998022824A1 (en) | Immunochromatography test strips | |
CA2021658A1 (en) | Multiplex immunoassay system | |
EP0374594A3 (en) | Test method and device for total protein assay | |
US5306622A (en) | Heterogeneous immunoassay process | |
WO1996019731A3 (en) | Differential timing method for detecting multiple analytes in a test sample | |
EP0380019A3 (en) | Methadone fluorescence polarization immunoassay | |
ES2076221T3 (en) | GEMINAL DERIVATIVES OF DIFENYL AND ITS USE IN IMMUNO ASSAYS. | |
CA2114440A1 (en) | Differential binding affinities and dissociation assays based thereon |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |