DE69130394D1 - Nicht-ätzende zusammensetzung und verfahren zur gewinnung von nukleinsäuren - Google Patents

Nicht-ätzende zusammensetzung und verfahren zur gewinnung von nukleinsäuren

Info

Publication number
DE69130394D1
DE69130394D1 DE69130394T DE69130394T DE69130394D1 DE 69130394 D1 DE69130394 D1 DE 69130394D1 DE 69130394 T DE69130394 T DE 69130394T DE 69130394 T DE69130394 T DE 69130394T DE 69130394 D1 DE69130394 D1 DE 69130394D1
Authority
DE
Germany
Prior art keywords
nucleic acids
sample
combined
extraction
benzyl alcohol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69130394T
Other languages
English (en)
Other versions
DE69130394T2 (de
Inventor
Ness Jeffrey Van
B Cimler
Rich Meyer
Nicholaas Vermeulen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanogen Inc
Original Assignee
MicroProbe Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/649,389 external-priority patent/US5130423A/en
Application filed by MicroProbe Corp filed Critical MicroProbe Corp
Publication of DE69130394D1 publication Critical patent/DE69130394D1/de
Application granted granted Critical
Publication of DE69130394T2 publication Critical patent/DE69130394T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69130394T 1990-07-13 1991-07-12 Nicht-ätzende zusammensetzung und verfahren zur gewinnung von nukleinsäuren Expired - Lifetime DE69130394T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US55274590A 1990-07-13 1990-07-13
US07/649,389 US5130423A (en) 1990-07-13 1991-02-01 Non-corrosive compositions and methods useful for the extraction of nucleic acids
PCT/US1991/004932 WO1992000983A1 (en) 1990-07-13 1991-07-12 Non-corrosive composition and methods useful for the extraction of nucleic acids

Publications (2)

Publication Number Publication Date
DE69130394D1 true DE69130394D1 (de) 1998-11-26
DE69130394T2 DE69130394T2 (de) 1999-04-08

Family

ID=27070123

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69130394T Expired - Lifetime DE69130394T2 (de) 1990-07-13 1991-07-12 Nicht-ätzende zusammensetzung und verfahren zur gewinnung von nukleinsäuren

Country Status (7)

Country Link
EP (1) EP0539515B1 (de)
JP (1) JP3102569B2 (de)
AT (1) ATE172466T1 (de)
CA (1) CA2087105C (de)
DE (1) DE69130394T2 (de)
ES (1) ES2125869T3 (de)
WO (1) WO1992000983A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5346994A (en) * 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
EP0680518B1 (de) * 1993-01-08 1997-04-16 HYBRIDON, Inc. Nachweis synthetischer oligonucleotide extrahiert aus körperflüssigkeiten oder geweben:
US5558992A (en) * 1993-01-08 1996-09-24 Hybridon, Inc. Detection of synthetic oligonucleotides extracted from body fluids or tissues
WO1996000228A1 (en) * 1994-06-23 1996-01-04 Dade International Inc. Method for the rapid isolation of nucleic acid
CA2153215A1 (en) * 1994-07-06 1996-01-07 Lu Wang Treatment of paraffin embedded tissue for gene analysis
US5945515A (en) * 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
DE69632904T2 (de) 1995-08-21 2004-11-18 Palma Bee'z Research Institute Co., Ltd. Kopräzipitat und methode zur extraktion von nukleinsäuren
ATE263254T1 (de) * 1995-10-02 2004-04-15 Emd Biosciences Inc Verfahren zur fällung von nukleinsäuren mit einem sichtbaren träger
US6242188B1 (en) 1999-07-30 2001-06-05 Applied Gene Technologies, Inc. Sample processing to release nucleic acids for direct detection
AU2000267686A1 (en) * 2000-08-10 2002-02-25 Applied Gene Technologies, Inc. Compositions and methods for nucleic acids sample processing and amplification
JP2005525819A (ja) * 2002-05-17 2005-09-02 ゲーエル ビオテック ゲーエムベーハー 核酸抽出および核酸精製のための方法
DE10222133A1 (de) * 2002-05-17 2003-12-04 Gl Biotech Gmbh Verfahren zur Nukleinsäure-Extraktion und Nukleinsäure-Reinigung
EP1633869A1 (de) * 2003-06-04 2006-03-15 Qiagen AS Verfahren zur sequenziellen isolierung von dns und rns aus derselben nukleinsäure-enthaltenden probe
EP1730302A2 (de) * 2004-02-27 2006-12-13 Stichting Laboratorium voor Infectieziekten Verfahren zum nachweis eines mikroorganismus in einer faecesprobe
KR101750179B1 (ko) * 2015-12-03 2017-07-03 최영옥 케이크 장식용 꽃꽂이 틀

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57140711A (en) * 1981-02-26 1982-08-31 Teika Seiyaku Kk Poultice for anti-inflammatory and analgesic use and its preparation

Also Published As

Publication number Publication date
DE69130394T2 (de) 1999-04-08
CA2087105C (en) 2000-09-12
ES2125869T3 (es) 1999-03-16
ATE172466T1 (de) 1998-11-15
EP0539515A1 (de) 1993-05-05
EP0539515B1 (de) 1998-10-21
WO1992000983A1 (en) 1992-01-23
CA2087105A1 (en) 1992-01-14
JP3102569B2 (ja) 2000-10-23
EP0539515A4 (de) 1995-04-26
JPH05508658A (ja) 1993-12-02

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