CA2210012A1 - Non-corrosive composition and methods useful for the extraction of nucleic acids - Google Patents
Non-corrosive composition and methods useful for the extraction of nucleic acidsInfo
- Publication number
- CA2210012A1 CA2210012A1 CA002210012A CA2210012A CA2210012A1 CA 2210012 A1 CA2210012 A1 CA 2210012A1 CA 002210012 A CA002210012 A CA 002210012A CA 2210012 A CA2210012 A CA 2210012A CA 2210012 A1 CA2210012 A1 CA 2210012A1
- Authority
- CA
- Canada
- Prior art keywords
- extraction
- nucleic acids
- sample
- combined
- methods useful
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
This invention relates to safe and effective methods for the extraction of nucleic acids. In particular, methods are described for isolating nucleic acid from a sample containing a biological mixture of nucleic acids and other biological compounds wherein the sample is combined with an extraction solution containing at least one organic compound such as benzyl alcohol or a benzyl alcohol derivative to form an aqueous and non-aqueous phase. The nucleic acid is isolated from the aqueous phase.
Preferably, the resulting combined solution also contains bentonite, as defined below. Typically, the sample will first be combined with a lysing agent before extraction.
The lysing agents preferred are chaotropic salts such as guanidinium hydrochloride and guanidinium isothiocyanate.
Preferably, the resulting combined solution also contains bentonite, as defined below. Typically, the sample will first be combined with a lysing agent before extraction.
The lysing agents preferred are chaotropic salts such as guanidinium hydrochloride and guanidinium isothiocyanate.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US55274590A | 1990-07-13 | 1990-07-13 | |
US552,745 | 1990-07-13 | ||
US649,389 | 1991-02-01 | ||
US07/649,389 US5130423A (en) | 1990-07-13 | 1991-02-01 | Non-corrosive compositions and methods useful for the extraction of nucleic acids |
CA002087105A CA2087105C (en) | 1990-07-13 | 1991-07-12 | Non-corrosive composition and methods useful for the extraction of nucleic acids |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002087105A Division CA2087105C (en) | 1990-07-13 | 1991-07-12 | Non-corrosive composition and methods useful for the extraction of nucleic acids |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2210012A1 true CA2210012A1 (en) | 1992-01-14 |
CA2210012C CA2210012C (en) | 2001-07-10 |
Family
ID=27169317
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002210012A Expired - Lifetime CA2210012C (en) | 1990-07-13 | 1991-07-12 | Non-corrosive composition and methods useful for the extraction of nucleic acids |
Country Status (1)
Country | Link |
---|---|
CA (1) | CA2210012C (en) |
-
1991
- 1991-07-12 CA CA002210012A patent/CA2210012C/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CA2210012C (en) | 2001-07-10 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |