CA2210012A1 - Non-corrosive composition and methods useful for the extraction of nucleic acids - Google Patents

Non-corrosive composition and methods useful for the extraction of nucleic acids

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Publication number
CA2210012A1
CA2210012A1 CA002210012A CA2210012A CA2210012A1 CA 2210012 A1 CA2210012 A1 CA 2210012A1 CA 002210012 A CA002210012 A CA 002210012A CA 2210012 A CA2210012 A CA 2210012A CA 2210012 A1 CA2210012 A1 CA 2210012A1
Authority
CA
Canada
Prior art keywords
extraction
nucleic acids
sample
combined
methods useful
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002210012A
Other languages
French (fr)
Other versions
CA2210012C (en
Inventor
Jeffrey Van Ness
B. Melina Cimler
Rich B. Meyer, Jr.
Nicholaas Marthinus Johannes Vermuelen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/649,389 external-priority patent/US5130423A/en
Application filed by Individual filed Critical Individual
Priority claimed from CA002087105A external-priority patent/CA2087105C/en
Publication of CA2210012A1 publication Critical patent/CA2210012A1/en
Application granted granted Critical
Publication of CA2210012C publication Critical patent/CA2210012C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

This invention relates to safe and effective methods for the extraction of nucleic acids. In particular, methods are described for isolating nucleic acid from a sample containing a biological mixture of nucleic acids and other biological compounds wherein the sample is combined with an extraction solution containing at least one organic compound such as benzyl alcohol or a benzyl alcohol derivative to form an aqueous and non-aqueous phase. The nucleic acid is isolated from the aqueous phase.
Preferably, the resulting combined solution also contains bentonite, as defined below. Typically, the sample will first be combined with a lysing agent before extraction.
The lysing agents preferred are chaotropic salts such as guanidinium hydrochloride and guanidinium isothiocyanate.
CA002210012A 1990-07-13 1991-07-12 Non-corrosive composition and methods useful for the extraction of nucleic acids Expired - Lifetime CA2210012C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US55274590A 1990-07-13 1990-07-13
US552,745 1990-07-13
US649,389 1991-02-01
US07/649,389 US5130423A (en) 1990-07-13 1991-02-01 Non-corrosive compositions and methods useful for the extraction of nucleic acids
CA002087105A CA2087105C (en) 1990-07-13 1991-07-12 Non-corrosive composition and methods useful for the extraction of nucleic acids

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA002087105A Division CA2087105C (en) 1990-07-13 1991-07-12 Non-corrosive composition and methods useful for the extraction of nucleic acids

Publications (2)

Publication Number Publication Date
CA2210012A1 true CA2210012A1 (en) 1992-01-14
CA2210012C CA2210012C (en) 2001-07-10

Family

ID=27169317

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002210012A Expired - Lifetime CA2210012C (en) 1990-07-13 1991-07-12 Non-corrosive composition and methods useful for the extraction of nucleic acids

Country Status (1)

Country Link
CA (1) CA2210012C (en)

Also Published As

Publication number Publication date
CA2210012C (en) 2001-07-10

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