DE69125263T2 - Verfahren für schnellen mikrobiologischen nachweis - Google Patents

Verfahren für schnellen mikrobiologischen nachweis

Info

Publication number
DE69125263T2
DE69125263T2 DE69125263T DE69125263T DE69125263T2 DE 69125263 T2 DE69125263 T2 DE 69125263T2 DE 69125263 T DE69125263 T DE 69125263T DE 69125263 T DE69125263 T DE 69125263T DE 69125263 T2 DE69125263 T2 DE 69125263T2
Authority
DE
Germany
Prior art keywords
bacteria
bacteriophage
sample
infect
indicator gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69125263T
Other languages
English (en)
Other versions
DE69125263D1 (de
Inventor
Catherine Elizabeth Dunn Rees
Katalin Rostas-Mulligan
Simon Fearon Park
Stephen Paul Denyer
Gordon Stewart
Sabah Abdel Amir Jassim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Foundation For Innovative New Diagnostics Gen Ch
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Publication of DE69125263D1 publication Critical patent/DE69125263D1/de
Application granted granted Critical
Publication of DE69125263T2 publication Critical patent/DE69125263T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C12N15/03—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE69125263T 1990-08-09 1991-08-08 Verfahren für schnellen mikrobiologischen nachweis Expired - Lifetime DE69125263T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB909017443A GB9017443D0 (en) 1990-08-09 1990-08-09 Reporter bacteria for rapid microbial detection
PCT/GB1991/001354 WO1992002633A1 (en) 1990-08-09 1991-08-08 Methods for rapid microbial detection

Publications (2)

Publication Number Publication Date
DE69125263D1 DE69125263D1 (de) 1997-04-24
DE69125263T2 true DE69125263T2 (de) 1997-09-18

Family

ID=10680380

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69125263T Expired - Lifetime DE69125263T2 (de) 1990-08-09 1991-08-08 Verfahren für schnellen mikrobiologischen nachweis

Country Status (10)

Country Link
US (2) US5498525A (de)
EP (1) EP0495960B1 (de)
JP (1) JP3088014B2 (de)
AT (1) ATE150489T1 (de)
CA (1) CA2066561C (de)
DE (1) DE69125263T2 (de)
DK (1) DK0495960T3 (de)
ES (1) ES2100955T3 (de)
GB (1) GB9017443D0 (de)
WO (1) WO1992002633A1 (de)

Families Citing this family (51)

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GB2295893B (en) * 1993-08-18 1997-10-08 Mini Agriculture & Fisheries Method and test kits for detection of bacteriophage
GB9317139D0 (en) * 1993-08-18 1993-10-06 Miniser For Agriculture Fisher Method and test kits for detection of bacteriophage
AU1716295A (en) * 1994-03-01 1995-09-18 Merck Patent Gmbh Selective virus culture
DE19517940A1 (de) * 1995-05-18 1996-11-21 Merck Patent Gmbh Nachweis von Listerien mittels rekombinanter Bakteriophagen
GB9525661D0 (en) * 1995-12-15 1996-02-14 Biotec Diagnostics Limited Method
US6461833B1 (en) 1995-12-15 2002-10-08 Biotec Laboratories Limited Method to detect bacteria
US6696286B1 (en) 1997-04-09 2004-02-24 3M Innovative Properties Company Method and devices for detecting and enumerating microorganisms
US6391578B2 (en) 1997-04-09 2002-05-21 3M Innovative Properties Company Method and devices for partitioning biological sample liquids into microvolumes
US5958675A (en) * 1997-04-18 1999-09-28 3M Innovative Properties Company Method for detecting bacteria using bacteriophage, contrast-coloring dye and precipitable dye
AU4850499A (en) 1998-06-29 2000-01-17 Procter & Gamble Company, The Device having fecal component sensor
US7132247B1 (en) 1998-09-17 2006-11-07 Regents Of The University Of Minnesota Composite devices incorporating biological material and methods
US6174699B1 (en) 1999-03-09 2001-01-16 3M Innovative Properties Company Disc assay device with inoculation pad and methods of use
US6501002B1 (en) 1999-06-29 2002-12-31 The Proctor & Gamble Company Disposable surface wipe article having a waste contamination sensor
US6391626B1 (en) 1999-08-23 2002-05-21 3M Innovative Properties Company Culture medium and device using ballasted pH indicators for detection and enumeration of microorganisms
US6737266B1 (en) 1999-10-01 2004-05-18 3M Innovative Properties Company Devices and methods for microorganism detection
US6436661B1 (en) * 2000-04-13 2002-08-20 3M Innovative Properties Company Bacteria and bacteriophage detection using immobilized enzyme substrates
US20020192676A1 (en) * 2001-06-18 2002-12-19 Madonna Angelo J. Method for determining if a type of bacteria is present in a mixture
AU2003299451A1 (en) * 2002-01-23 2004-06-07 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health Andhuman Services Method for determining sensitivity to a bacteriophage
AU2003228505B2 (en) * 2002-04-12 2009-01-08 Colorado School Of Mines Method for detecting low concentrations of a target bacterium that uses phages to infect target bacterial cells
US20050003346A1 (en) * 2002-04-12 2005-01-06 Colorado School Of Mines Apparatus and method for detecting microscopic living organisms using bacteriophage
US8216780B2 (en) 2002-04-12 2012-07-10 Microphage (Tm) Incorporated Method for enhanced sensitivity in bacteriophage-based diagnostic assays
WO2005001475A2 (en) 2003-04-10 2005-01-06 Kent Voorhees Apparatus and method for detecting microscopic living organisms using bacteriophage
WO2005014805A1 (en) * 2003-08-08 2005-02-17 Regents Of The University Of Minnesota A structured material for the production of hydrogen
US20090286225A1 (en) * 2004-02-13 2009-11-19 Microphagetm Incorporated Method and apparatus for bacteriophage-based diagnostic assays
US20050250096A1 (en) * 2004-02-13 2005-11-10 Microphage, Incorporated Microorganism detection using bacteriophage amplification
ES2523316T3 (es) * 2004-11-01 2014-11-24 Erber Aktiengesellschaft Bacteriófagos como agentes selectivos
US20110097702A1 (en) * 2005-03-31 2011-04-28 Voorhees Kent J Methods and compositions for in situ detection of microorganisms on a surface
WO2006105504A1 (en) 2005-03-31 2006-10-05 Microphage Incorporated Apparatus and method for detecting microorganisms using flagged bacteriophage
EP1885853A2 (de) * 2005-05-27 2008-02-13 Guild Associates, Inc. Biologisches detektionssystem und -verfahren
US20070178450A1 (en) * 2006-01-27 2007-08-02 Microphage (Tm) Incorporation Method and apparatus for determining level of microorganisms using bacteriophage
EP2087130A2 (de) * 2006-10-31 2009-08-12 Microphage, Incorporated Verfahren und vorrichtung für erweiterte bakteriophagen-basierte diagnose-assays durch selektive hemmung potentiell kreuzreaktiver organismen
EP2140018B1 (de) * 2007-04-04 2012-08-01 Guild Associates, Inc. Biologisches detektionssystem und -verfahren
CA2690809A1 (en) 2007-06-15 2008-12-24 Microphage Incorporated Method of detection of microorganisms with enhanced bacteriophage amplification
CN101329342A (zh) * 2007-06-22 2008-12-24 王霁 检测结核菌和抗结核药物敏感性的方法及试剂盒
US8697434B2 (en) * 2008-01-11 2014-04-15 Colorado School Of Mines Detection of phage amplification by SERS nanoparticles
US9441204B2 (en) * 2008-04-03 2016-09-13 Colorado School Of Mines Compositions and methods for detecting Yersinia pestis bacteria
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
US20100112630A1 (en) * 2008-11-03 2010-05-06 Scott Martell Boyette Methods for measuring microbiological content in aqueous media
US20110183314A1 (en) * 2010-01-26 2011-07-28 Microphage Incorporated Bacteriophage-based microorganism diagnostic assay using speed or acceleration of bacteriophage reproduction
GB2477752A (en) 2010-02-11 2011-08-17 Arab Biotechnology Company Detection of bacteria
CN110938671A (zh) 2013-03-13 2020-03-31 经纬生物科技有限公司 非复制型转导颗粒和基于转导颗粒的报告系统
US9481903B2 (en) 2013-03-13 2016-11-01 Roche Molecular Systems, Inc. Systems and methods for detection of cells using engineered transduction particles
EP2970917A2 (de) 2013-03-15 2016-01-20 Altria Client Services LLC Bakteriophage und verfahren zur herstellung und verwendung
US8945837B2 (en) 2013-03-15 2015-02-03 American Sterilizer Company Sterilization indicator including a simplified genetically engineered biological indicator
US9340817B2 (en) 2013-03-27 2016-05-17 Sample6 Technologies, Inc. Methods of making recombinant phage, compositions and articles of manufacture of same for bacterial detection
EP3011062A4 (de) * 2013-06-19 2017-06-14 Sample6 Technologies Inc. Bakteriendetektionstest auf basis von phagen
ES2731325T3 (es) 2013-10-01 2019-11-15 Univ Nottingham Detección de micobacterias usando bacteriófagos
US9540675B2 (en) 2013-10-29 2017-01-10 GeneWeave Biosciences, Inc. Reagent cartridge and methods for detection of cells
WO2016123425A1 (en) 2015-01-29 2016-08-04 Altria Client Services Llc Endolysin from bacteriophage against geobacillus and methods of using
WO2016123427A1 (en) 2015-01-29 2016-08-04 Altria Client Services Llc Bacteriophage and methods of using
US10351893B2 (en) 2015-10-05 2019-07-16 GeneWeave Biosciences, Inc. Reagent cartridge for detection of cells

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1277931C (en) * 1984-06-05 1990-12-18 Shimon Ulitzur Detection and/or identification of microorganisms in a test sample usingbioluminescence or other exogenous genetically-introduced marker
FR2621924B1 (fr) * 1987-10-19 1990-01-12 Pasteur Institut Gene recombinant, notamment retrovirus recombinant defectif et activable par un transactivateur, cellules les contenant, leur procede de fabrication et leurs applications, notamment pour la detection in vitro d'une infection retrovirale ou de l'etude de l'inhibition in vitro de l'infection par des principes actifs de medicaments
DE3833628A1 (de) * 1988-10-03 1990-04-12 Genlux Forschungsgesellschaft Verfahren zum nachweis und zur identifikation toxischer substanzen mit hilfe klonierter mikroorganismen
EP0437537B1 (de) * 1988-10-04 1997-02-26 DNA Plant Technology Corporation Bakterieller nachweis durch phagentransduktion von nachweisbaren phenotypen

Also Published As

Publication number Publication date
CA2066561A1 (en) 1992-02-10
DK0495960T3 (da) 1997-08-25
ES2100955T3 (es) 1997-07-01
EP0495960B1 (de) 1997-03-19
US5723330A (en) 1998-03-03
CA2066561C (en) 2003-10-14
JPH05501958A (ja) 1993-04-15
DE69125263D1 (de) 1997-04-24
WO1992002633A1 (en) 1992-02-20
US5498525A (en) 1996-03-12
EP0495960A1 (de) 1992-07-29
ATE150489T1 (de) 1997-04-15
JP3088014B2 (ja) 2000-09-18
GB9017443D0 (en) 1990-09-26

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: FOUNDATION FOR INNOVATIVE NEW DIAGNOSTICS, GEN, CH

8328 Change in the person/name/address of the agent

Representative=s name: MEISSNER, BOLTE & PARTNER GBR, 81679 MUENCHEN

8328 Change in the person/name/address of the agent

Representative=s name: MEISSNER, BOLTE & PARTNER GBR, 80538 MUENCHEN