ES2100955T3 - Metodo para deteccion microbiana rapida. - Google Patents

Metodo para deteccion microbiana rapida.

Info

Publication number
ES2100955T3
ES2100955T3 ES91914850T ES91914850T ES2100955T3 ES 2100955 T3 ES2100955 T3 ES 2100955T3 ES 91914850 T ES91914850 T ES 91914850T ES 91914850 T ES91914850 T ES 91914850T ES 2100955 T3 ES2100955 T3 ES 2100955T3
Authority
ES
Spain
Prior art keywords
bacteria
bacteriophage
sample
detection method
infect
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91914850T
Other languages
English (en)
Inventor
Catherine Elizabeth Dunn Rees
Katalin Rostas-Mulligan
Simon Fearon Park
Stephen Paul Denyer
Gordon Sydney Anderson Stewart
Sabah Abdel Amir Jassim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Application granted granted Critical
Publication of ES2100955T3 publication Critical patent/ES2100955T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C12N15/03—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

UN METODO PARA ANALIZAR UNA BACTERIA DE BLANCO QUE CONSISTE EN AÑADIR UN BACTERIOLAGO A UNA MUESTRA PARA INFECTAR LA BACTERIA QUE SE ENCUENTRA EN LA MUESTRA; MATAR EL BACTERIOLAGO EXTRACELULAR SIN MATAR AL MISMO TIEMPO LA BACTERIA INFECTADA CON EL LAGO; AMPLIFICAR EL BACTERIOLAGO QUE QUEDA EN LA MUESTRA; Y HACER QUE EL BACTERIOLAGO INFECTE UNA BACTERIA REPORTERA Y ASI PRODUCIR UNA SEÑAL VISIBLE. LAS BACTERIAS REPORTERAS SON SOMETIDAS A UNA INGENIERIA GENETICA PARA TENER UN GEN INDICADOR QUE AL EXPRESARSE DA LUGAR A UNA SEÑAL DETECTABLE EN DONDE LA EXPRESION DEL GEN INDICADOR SE INICIA EN UNA INFECCION DEL BACTERIOLAGO DE LA BACTERIA.
ES91914850T 1990-08-09 1991-08-08 Metodo para deteccion microbiana rapida. Expired - Lifetime ES2100955T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB909017443A GB9017443D0 (en) 1990-08-09 1990-08-09 Reporter bacteria for rapid microbial detection

Publications (1)

Publication Number Publication Date
ES2100955T3 true ES2100955T3 (es) 1997-07-01

Family

ID=10680380

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91914850T Expired - Lifetime ES2100955T3 (es) 1990-08-09 1991-08-08 Metodo para deteccion microbiana rapida.

Country Status (10)

Country Link
US (2) US5498525A (es)
EP (1) EP0495960B1 (es)
JP (1) JP3088014B2 (es)
AT (1) ATE150489T1 (es)
CA (1) CA2066561C (es)
DE (1) DE69125263T2 (es)
DK (1) DK0495960T3 (es)
ES (1) ES2100955T3 (es)
GB (1) GB9017443D0 (es)
WO (1) WO1992002633A1 (es)

Families Citing this family (51)

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GB2295893B (en) * 1993-08-18 1997-10-08 Mini Agriculture & Fisheries Method and test kits for detection of bacteriophage
GB9317139D0 (en) * 1993-08-18 1993-10-06 Miniser For Agriculture Fisher Method and test kits for detection of bacteriophage
KR100373798B1 (ko) * 1994-03-01 2003-12-31 메르크 파텐트 게엠베하 선택적인바이러스배양
DE19517940A1 (de) * 1995-05-18 1996-11-21 Merck Patent Gmbh Nachweis von Listerien mittels rekombinanter Bakteriophagen
US6461833B1 (en) 1995-12-15 2002-10-08 Biotec Laboratories Limited Method to detect bacteria
GB9525661D0 (en) * 1995-12-15 1996-02-14 Biotec Diagnostics Limited Method
US6696286B1 (en) 1997-04-09 2004-02-24 3M Innovative Properties Company Method and devices for detecting and enumerating microorganisms
US6391578B2 (en) 1997-04-09 2002-05-21 3M Innovative Properties Company Method and devices for partitioning biological sample liquids into microvolumes
US5958675A (en) * 1997-04-18 1999-09-28 3M Innovative Properties Company Method for detecting bacteria using bacteriophage, contrast-coloring dye and precipitable dye
AU4840899A (en) 1998-06-29 2000-01-17 Procter & Gamble Company, The Disposable article having proactive sensor
US7132247B1 (en) 1998-09-17 2006-11-07 Regents Of The University Of Minnesota Composite devices incorporating biological material and methods
US6174699B1 (en) 1999-03-09 2001-01-16 3M Innovative Properties Company Disc assay device with inoculation pad and methods of use
US6501002B1 (en) 1999-06-29 2002-12-31 The Proctor & Gamble Company Disposable surface wipe article having a waste contamination sensor
US6391626B1 (en) 1999-08-23 2002-05-21 3M Innovative Properties Company Culture medium and device using ballasted pH indicators for detection and enumeration of microorganisms
US6737266B1 (en) 1999-10-01 2004-05-18 3M Innovative Properties Company Devices and methods for microorganism detection
US6436661B1 (en) * 2000-04-13 2002-08-20 3M Innovative Properties Company Bacteria and bacteriophage detection using immobilized enzyme substrates
US20020192676A1 (en) * 2001-06-18 2002-12-19 Madonna Angelo J. Method for determining if a type of bacteria is present in a mixture
US20050118567A1 (en) * 2002-01-23 2005-06-02 Merril Carl R. Method for determining sensitivity to a bacteriophage
US20050003346A1 (en) * 2002-04-12 2005-01-06 Colorado School Of Mines Apparatus and method for detecting microscopic living organisms using bacteriophage
US8216780B2 (en) 2002-04-12 2012-07-10 Microphage (Tm) Incorporated Method for enhanced sensitivity in bacteriophage-based diagnostic assays
EP1540018B1 (en) * 2002-04-12 2010-05-26 Colorado School Of Mines Method for detecting low concentrations of a target bacterium that uses phages to infect target bacterial cells
WO2005001475A2 (en) 2003-04-10 2005-01-06 Kent Voorhees Apparatus and method for detecting microscopic living organisms using bacteriophage
US7745023B2 (en) * 2003-08-08 2010-06-29 Regents Of The University Of Minnesota Structured material for the production of hydrogen
US20090286225A1 (en) * 2004-02-13 2009-11-19 Microphagetm Incorporated Method and apparatus for bacteriophage-based diagnostic assays
US20050250096A1 (en) * 2004-02-13 2005-11-10 Microphage, Incorporated Microorganism detection using bacteriophage amplification
CA2586299C (en) * 2004-11-01 2014-10-28 James W. Stave Method for detecting or enriching a target microorganism upon lysis of non-target bacteria with bacteriophage
US20110097702A1 (en) * 2005-03-31 2011-04-28 Voorhees Kent J Methods and compositions for in situ detection of microorganisms on a surface
WO2006105414A2 (en) 2005-03-31 2006-10-05 Colorado School Of Mines Apparatus and method for detecting microscopic organisms using microphage
US20080193917A1 (en) * 2005-05-27 2008-08-14 David Alexander Schofield Biological Detection System and Method
US20070178450A1 (en) * 2006-01-27 2007-08-02 Microphage (Tm) Incorporation Method and apparatus for determining level of microorganisms using bacteriophage
WO2008063838A2 (en) * 2006-10-31 2008-05-29 Microphage Incorporated Method and apparatus for enhanced bacteriophage-based diagnostic assays by selective inhibition of potential cross-reactive organisms
EP2140018B1 (en) * 2007-04-04 2012-08-01 Guild Associates, Inc. Biological detection system and method
EP2158490A2 (en) 2007-06-15 2010-03-03 Microphage, Incorporated Method of detection of microorganisms with enhanced bacteriophage amplification
CN101329342A (zh) * 2007-06-22 2008-12-24 王霁 检测结核菌和抗结核药物敏感性的方法及试剂盒
US8697434B2 (en) * 2008-01-11 2014-04-15 Colorado School Of Mines Detection of phage amplification by SERS nanoparticles
US9441204B2 (en) * 2008-04-03 2016-09-13 Colorado School Of Mines Compositions and methods for detecting Yersinia pestis bacteria
US20100112630A1 (en) * 2008-11-03 2010-05-06 Scott Martell Boyette Methods for measuring microbiological content in aqueous media
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
US20110183314A1 (en) * 2010-01-26 2011-07-28 Microphage Incorporated Bacteriophage-based microorganism diagnostic assay using speed or acceleration of bacteriophage reproduction
GB2477752A (en) 2010-02-11 2011-08-17 Arab Biotechnology Company Detection of bacteria
US9481903B2 (en) 2013-03-13 2016-11-01 Roche Molecular Systems, Inc. Systems and methods for detection of cells using engineered transduction particles
WO2014160418A2 (en) 2013-03-13 2014-10-02 GeneWeave Biosciences, Inc. Non-replicative transduction particles and transduction particle-based reporter systems
US8945837B2 (en) 2013-03-15 2015-02-03 American Sterilizer Company Sterilization indicator including a simplified genetically engineered biological indicator
WO2014150870A2 (en) 2013-03-15 2014-09-25 Altria Client Services Inc. Bacteriophage and methods of making and using
EP2978857A2 (en) 2013-03-27 2016-02-03 Sample Technologies, Inc. Recombinant phage and bacterial detection methods
AU2014281406A1 (en) * 2013-06-19 2016-01-21 Sample6 Technologies, Inc. Phage-based bacterial detection assay
PL3052650T3 (pl) 2013-10-01 2019-09-30 The University Of Nottingham Wykrywanie mykobakterii za pomocą bakteriofagów
US9540675B2 (en) 2013-10-29 2017-01-10 GeneWeave Biosciences, Inc. Reagent cartridge and methods for detection of cells
WO2016123425A1 (en) 2015-01-29 2016-08-04 Altria Client Services Llc Endolysin from bacteriophage against geobacillus and methods of using
US9781929B2 (en) 2015-01-29 2017-10-10 Altria Client Services Llc Bacteriophage and methods of using
US10351893B2 (en) 2015-10-05 2019-07-16 GeneWeave Biosciences, Inc. Reagent cartridge for detection of cells

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1277931C (en) * 1984-06-05 1990-12-18 Shimon Ulitzur Detection and/or identification of microorganisms in a test sample usingbioluminescence or other exogenous genetically-introduced marker
FR2621924B1 (fr) * 1987-10-19 1990-01-12 Pasteur Institut Gene recombinant, notamment retrovirus recombinant defectif et activable par un transactivateur, cellules les contenant, leur procede de fabrication et leurs applications, notamment pour la detection in vitro d'une infection retrovirale ou de l'etude de l'inhibition in vitro de l'infection par des principes actifs de medicaments
DE3833628A1 (de) * 1988-10-03 1990-04-12 Genlux Forschungsgesellschaft Verfahren zum nachweis und zur identifikation toxischer substanzen mit hilfe klonierter mikroorganismen
DE68927803T2 (de) * 1988-10-04 1997-07-10 Dna Plant Techn Corp Bakterieller nachweis durch phagentransduktion von nachweisbaren phenotypen

Also Published As

Publication number Publication date
WO1992002633A1 (en) 1992-02-20
US5723330A (en) 1998-03-03
DE69125263D1 (de) 1997-04-24
DK0495960T3 (da) 1997-08-25
EP0495960A1 (en) 1992-07-29
JP3088014B2 (ja) 2000-09-18
GB9017443D0 (en) 1990-09-26
ATE150489T1 (de) 1997-04-15
EP0495960B1 (en) 1997-03-19
DE69125263T2 (de) 1997-09-18
CA2066561C (en) 2003-10-14
JPH05501958A (ja) 1993-04-15
CA2066561A1 (en) 1992-02-10
US5498525A (en) 1996-03-12

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