ATE150489T1 - Verfahren für schnellen mikrobiologischen nachweis - Google Patents

Verfahren für schnellen mikrobiologischen nachweis

Info

Publication number
ATE150489T1
ATE150489T1 AT91914850T AT91914850T ATE150489T1 AT E150489 T1 ATE150489 T1 AT E150489T1 AT 91914850 T AT91914850 T AT 91914850T AT 91914850 T AT91914850 T AT 91914850T AT E150489 T1 ATE150489 T1 AT E150489T1
Authority
AT
Austria
Prior art keywords
bacteria
bacteriophage
sample
infect
indicator gene
Prior art date
Application number
AT91914850T
Other languages
English (en)
Inventor
Catherine Elizabeth Dunn Rees
Katalin Rostas-Mulligan
Simon Fearon Park
Stephen Paul Denyer
Gordon Sydney Anderson Stewart
Sabah Abdel Amir Jassim
Original Assignee
Merck Patent Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh
Application granted granted Critical
Publication of ATE150489T1 publication Critical patent/ATE150489T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C12N15/03—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT91914850T 1990-08-09 1991-08-08 Verfahren für schnellen mikrobiologischen nachweis ATE150489T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB909017443A GB9017443D0 (en) 1990-08-09 1990-08-09 Reporter bacteria for rapid microbial detection

Publications (1)

Publication Number Publication Date
ATE150489T1 true ATE150489T1 (de) 1997-04-15

Family

ID=10680380

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91914850T ATE150489T1 (de) 1990-08-09 1991-08-08 Verfahren für schnellen mikrobiologischen nachweis

Country Status (10)

Country Link
US (2) US5498525A (de)
EP (1) EP0495960B1 (de)
JP (1) JP3088014B2 (de)
AT (1) ATE150489T1 (de)
CA (1) CA2066561C (de)
DE (1) DE69125263T2 (de)
DK (1) DK0495960T3 (de)
ES (1) ES2100955T3 (de)
GB (1) GB9017443D0 (de)
WO (1) WO1992002633A1 (de)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2295893B (en) * 1993-08-18 1997-10-08 Mini Agriculture & Fisheries Method and test kits for detection of bacteriophage
GB9317139D0 (en) * 1993-08-18 1993-10-06 Miniser For Agriculture Fisher Method and test kits for detection of bacteriophage
KR100373798B1 (ko) * 1994-03-01 2003-12-31 메르크 파텐트 게엠베하 선택적인바이러스배양
DE19517940A1 (de) * 1995-05-18 1996-11-21 Merck Patent Gmbh Nachweis von Listerien mittels rekombinanter Bakteriophagen
GB9525661D0 (en) * 1995-12-15 1996-02-14 Biotec Diagnostics Limited Method
US6461833B1 (en) 1995-12-15 2002-10-08 Biotec Laboratories Limited Method to detect bacteria
US6391578B2 (en) 1997-04-09 2002-05-21 3M Innovative Properties Company Method and devices for partitioning biological sample liquids into microvolumes
US6696286B1 (en) 1997-04-09 2004-02-24 3M Innovative Properties Company Method and devices for detecting and enumerating microorganisms
US5958675A (en) * 1997-04-18 1999-09-28 3M Innovative Properties Company Method for detecting bacteria using bacteriophage, contrast-coloring dye and precipitable dye
ES2293728T3 (es) 1998-06-29 2008-03-16 THE PROCTER & GAMBLE COMPANY Articulo de tratamiento desechable que tiene un sistema sensible.
US7132247B1 (en) 1998-09-17 2006-11-07 Regents Of The University Of Minnesota Composite devices incorporating biological material and methods
US6174699B1 (en) 1999-03-09 2001-01-16 3M Innovative Properties Company Disc assay device with inoculation pad and methods of use
US6501002B1 (en) 1999-06-29 2002-12-31 The Proctor & Gamble Company Disposable surface wipe article having a waste contamination sensor
US6391626B1 (en) 1999-08-23 2002-05-21 3M Innovative Properties Company Culture medium and device using ballasted pH indicators for detection and enumeration of microorganisms
US6737266B1 (en) 1999-10-01 2004-05-18 3M Innovative Properties Company Devices and methods for microorganism detection
US6436661B1 (en) * 2000-04-13 2002-08-20 3M Innovative Properties Company Bacteria and bacteriophage detection using immobilized enzyme substrates
US20020192676A1 (en) * 2001-06-18 2002-12-19 Madonna Angelo J. Method for determining if a type of bacteria is present in a mixture
WO2004041156A2 (en) * 2002-01-23 2004-05-21 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health Andhuman Services Method for determining sensitivity to a bacteriophage
JP4490112B2 (ja) * 2002-04-12 2010-06-23 コロラド・スクール・オブ・マインズ 標的細菌性細胞を感染させるためにファージを使用する低濃度の標的細菌を検出するための方法
US8216780B2 (en) 2002-04-12 2012-07-10 Microphage (Tm) Incorporated Method for enhanced sensitivity in bacteriophage-based diagnostic assays
US20050003346A1 (en) * 2002-04-12 2005-01-06 Colorado School Of Mines Apparatus and method for detecting microscopic living organisms using bacteriophage
EP1613965A2 (de) 2003-04-10 2006-01-11 Kent J. Voorhees Vorrichtung und verfahren zum nachweis mikroskopischer lebender organismen mittels bakteriophagen
US7745023B2 (en) * 2003-08-08 2010-06-29 Regents Of The University Of Minnesota Structured material for the production of hydrogen
US20090286225A1 (en) * 2004-02-13 2009-11-19 Microphagetm Incorporated Method and apparatus for bacteriophage-based diagnostic assays
US20050250096A1 (en) * 2004-02-13 2005-11-10 Microphage, Incorporated Microorganism detection using bacteriophage amplification
AU2005302390A1 (en) * 2004-11-01 2006-05-11 Strategic Diagnostics, Inc. Bacteriophages as selective agents
US20110097702A1 (en) * 2005-03-31 2011-04-28 Voorhees Kent J Methods and compositions for in situ detection of microorganisms on a surface
WO2006105504A1 (en) * 2005-03-31 2006-10-05 Microphage Incorporated Apparatus and method for detecting microorganisms using flagged bacteriophage
WO2007055737A2 (en) * 2005-05-27 2007-05-18 Guild Associates, Inc. Biological detection system and method
US20070178450A1 (en) * 2006-01-27 2007-08-02 Microphage (Tm) Incorporation Method and apparatus for determining level of microorganisms using bacteriophage
JP2010508044A (ja) * 2006-10-31 2010-03-18 マイクロファージ・インコーポレーテッド 潜在的に交差反応性の生物の選択阻害による、強化されたバクテリオファージベース診断アッセイの方法及び装置
WO2009005869A2 (en) * 2007-04-04 2009-01-08 Guild Associates, Inc. Biological detection system and method
US8455186B2 (en) * 2007-06-15 2013-06-04 MicroPhage™ Incorporated Method of detection of microorganisms with enhanced bacteriophage amplification
CN101329342A (zh) * 2007-06-22 2008-12-24 王霁 检测结核菌和抗结核药物敏感性的方法及试剂盒
US8697434B2 (en) * 2008-01-11 2014-04-15 Colorado School Of Mines Detection of phage amplification by SERS nanoparticles
US9441204B2 (en) * 2008-04-03 2016-09-13 Colorado School Of Mines Compositions and methods for detecting Yersinia pestis bacteria
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
US20100112630A1 (en) * 2008-11-03 2010-05-06 Scott Martell Boyette Methods for measuring microbiological content in aqueous media
US20110183314A1 (en) * 2010-01-26 2011-07-28 Microphage Incorporated Bacteriophage-based microorganism diagnostic assay using speed or acceleration of bacteriophage reproduction
GB2477752A (en) 2010-02-11 2011-08-17 Arab Biotechnology Company Detection of bacteria
KR102127358B1 (ko) 2013-03-13 2020-06-29 지니위브 바이오사이언시스, 인코포레이티드 비-복제 형질도입 입자 및 형질도입 입자-기반 리포터 시스템
US9481903B2 (en) 2013-03-13 2016-11-01 Roche Molecular Systems, Inc. Systems and methods for detection of cells using engineered transduction particles
US8945837B2 (en) 2013-03-15 2015-02-03 American Sterilizer Company Sterilization indicator including a simplified genetically engineered biological indicator
WO2014150870A2 (en) 2013-03-15 2014-09-25 Altria Client Services Inc. Bacteriophage and methods of making and using
AU2014241191A1 (en) 2013-03-27 2015-10-22 Sample Technologies, Inc. Recombinant phage and bacterial detection methods
AU2014281406A1 (en) 2013-06-19 2016-01-21 Sample6 Technologies, Inc. Phage-based bacterial detection assay
PL3052650T3 (pl) 2013-10-01 2019-09-30 The University Of Nottingham Wykrywanie mykobakterii za pomocą bakteriofagów
US9540675B2 (en) 2013-10-29 2017-01-10 GeneWeave Biosciences, Inc. Reagent cartridge and methods for detection of cells
WO2016123425A1 (en) 2015-01-29 2016-08-04 Altria Client Services Llc Endolysin from bacteriophage against geobacillus and methods of using
WO2016123427A1 (en) 2015-01-29 2016-08-04 Altria Client Services Llc Bacteriophage and methods of using
US10351893B2 (en) 2015-10-05 2019-07-16 GeneWeave Biosciences, Inc. Reagent cartridge for detection of cells

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1277931C (en) * 1984-06-05 1990-12-18 Shimon Ulitzur Detection and/or identification of microorganisms in a test sample usingbioluminescence or other exogenous genetically-introduced marker
FR2621924B1 (fr) * 1987-10-19 1990-01-12 Pasteur Institut Gene recombinant, notamment retrovirus recombinant defectif et activable par un transactivateur, cellules les contenant, leur procede de fabrication et leurs applications, notamment pour la detection in vitro d'une infection retrovirale ou de l'etude de l'inhibition in vitro de l'infection par des principes actifs de medicaments
DE3833628A1 (de) * 1988-10-03 1990-04-12 Genlux Forschungsgesellschaft Verfahren zum nachweis und zur identifikation toxischer substanzen mit hilfe klonierter mikroorganismen
AU634500B2 (en) * 1988-10-04 1993-02-25 Dna Plant Technology Corporation Bacterial detection by phage transduction of detectable phenotype

Also Published As

Publication number Publication date
GB9017443D0 (en) 1990-09-26
US5498525A (en) 1996-03-12
US5723330A (en) 1998-03-03
EP0495960B1 (de) 1997-03-19
ES2100955T3 (es) 1997-07-01
WO1992002633A1 (en) 1992-02-20
JPH05501958A (ja) 1993-04-15
DE69125263T2 (de) 1997-09-18
CA2066561A1 (en) 1992-02-10
DK0495960T3 (da) 1997-08-25
JP3088014B2 (ja) 2000-09-18
CA2066561C (en) 2003-10-14
DE69125263D1 (de) 1997-04-24
EP0495960A1 (de) 1992-07-29

Similar Documents

Publication Publication Date Title
DE69125263D1 (de) Verfahren für schnellen mikrobiologischen nachweis
Oliveira et al. Evaluation of selective and non‐selective enrichment PCR procedures for Salmonella detection
BRPI0208597B8 (pt) meio de detecção de staphylococcus aureus e/ou estafilococos com coagulose positiva e método de identificação de bactéria (s) da espécie staphylococcus aureus em uma amostra
NO964187L (no) Fremgangsmåte for fremstilling av mykobakterier
MX9704281A (es) Metodo de deteccion de acidos nucleicos con una composicion de secuencia especifica.
DE69231646D1 (de) Fingerabdruckartige identifikation von bakterienstämmen mittels amplifikation repetitiver dna-sequenzen
DE68927803D1 (de) Bakterieller nachweis durch phagentransduktion von nachweisbaren phenotypen
DE69624593D1 (de) Verfahren zum nachweis von bakterien
ES8900020A1 (es) Un metodo para ensayar composiciones o mezclas de antibioticos, con relacion a su actividad,en un medio
ES423171A1 (es) Procedimiento para el ensayo de la presencia de microorga- nismos en un ser viviente.
DE69833660D1 (de) Nukleinsäuresonden zur detektion von candida-spezies
FI971802A0 (fi) Mycobacterium avium-kompleksilajien monistus ja ilmaisu
ES2157569T3 (es) Procedimiento de deteccion y/o cuantificacion de un factor gliotoxico.
Miles et al. The relation of toxicity and enzyme activity in the lecithinases of clostridium bifermentans and clostridium welchii
Buchanan Clinical laboratory evaluation of a reverse CAMP test for presumptive identification of Clostridium perfringens
Bashiruddin et al. Detection of Mycoplasma mycoides subspecies mycoides in tissues from an outbreak of contagious bovine pleuropneumonia by culture, immunohistochemistry and polymerase chain reaction
ATE464399T1 (de) Verwendung der photopolymerisierung zur amplifikation und zum nachweis eines molekularen erkennungsereignisses
ES2186889T3 (es) Sistema de linea celular de marcado que permite detectar la presencia de citomegalovirus e identificar moduladores de la expresion de genes virales.
DE69837768D1 (de) Verfahren und agens zur bestimmung von deaminase-aktivität
DK1155148T3 (da) Detektering og kvantificering af mikroorganismer under anvendelse af amplifikation og restriktionsenzymanalyse
ES2043674T3 (es) Procedimiento de evaluacion mutagenica.
EP0904410A4 (de) Erkennung von pyrazinamid-resistenten mycobakterien und verfahren zur behandlung von mycobakterien-infektionen
Parnas Differentiation of brucellae by the aid of phages
Chue-Gonçalves et al. New approach for detection of Escherichia coli invasion to HeLa cells
Endo et al. Quantitative detection of genetically-modified micro Organisms by the PCR-MPN method