DE69116704D1 - Gen, das ein Polypeptid mit Nitrilhydratase-Aktivität codiert, eine dieses Gen enthaltende Transformante und Verfahren zur Herstellung von Amiden mit dieser Transformante. - Google Patents

Gen, das ein Polypeptid mit Nitrilhydratase-Aktivität codiert, eine dieses Gen enthaltende Transformante und Verfahren zur Herstellung von Amiden mit dieser Transformante.

Info

Publication number
DE69116704D1
DE69116704D1 DE69116704T DE69116704T DE69116704D1 DE 69116704 D1 DE69116704 D1 DE 69116704D1 DE 69116704 T DE69116704 T DE 69116704T DE 69116704 T DE69116704 T DE 69116704T DE 69116704 D1 DE69116704 D1 DE 69116704D1
Authority
DE
Germany
Prior art keywords
transformant
gene
polypeptide
nitrile hydratase
hydratase activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69116704T
Other languages
English (en)
Other versions
DE69116704T2 (de
Inventor
Teruhiko Beppu
Hideaki Yamada
Toru Nagasawa
Sueharu Horinouchi
Makoto Nishiyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Rayon Co Ltd
Original Assignee
Nippon Chemical Industrial Co Ltd
Nitto Chemical Industry Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Chemical Industrial Co Ltd, Nitto Chemical Industry Co Ltd filed Critical Nippon Chemical Industrial Co Ltd
Application granted granted Critical
Publication of DE69116704D1 publication Critical patent/DE69116704D1/de
Publication of DE69116704T2 publication Critical patent/DE69116704T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69116704T 1990-02-28 1991-02-27 Gen, das ein Polypeptid mit Nitrilhydratase-Aktivität codiert, eine dieses Gen enthaltende Transformante und Verfahren zur Herstellung von Amiden mit dieser Transformante. Expired - Lifetime DE69116704T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2048079A JP2907479B2 (ja) 1990-02-28 1990-02-28 ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体及びアミド類の製造法

Publications (2)

Publication Number Publication Date
DE69116704D1 true DE69116704D1 (de) 1996-03-14
DE69116704T2 DE69116704T2 (de) 1996-08-08

Family

ID=12793330

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69116704T Expired - Lifetime DE69116704T2 (de) 1990-02-28 1991-02-27 Gen, das ein Polypeptid mit Nitrilhydratase-Aktivität codiert, eine dieses Gen enthaltende Transformante und Verfahren zur Herstellung von Amiden mit dieser Transformante.

Country Status (7)

Country Link
EP (1) EP0444639B1 (de)
JP (1) JP2907479B2 (de)
KR (1) KR950000888B1 (de)
CN (1) CN1052509C (de)
AU (1) AU636008B2 (de)
DE (1) DE69116704T2 (de)
RU (2) RU2028380C1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3692593A (en) * 1992-04-28 1993-11-04 Mitsubishi Kasei Corporation Novel protein having nitrile hydratase activity and the gene encoding the same, and a method for producing amides from nitriles via a transformant containing the gene
WO1994006930A1 (en) * 1992-09-21 1994-03-31 E.I. Du Pont De Nemuors And Company A process for the preparation of enantiomeric 2-alkanoic acids
FR2708936B1 (fr) * 1993-08-10 1995-11-10 Rhone Poulenc Chimie Enzymes à activité nitrile-hydratase, outils génétiques et micro-organismes hôtes permettant leur obtention et procédé d'hydrolyse mettant en Óoeuvre lesdites enzymes.
CN1243825C (zh) * 1996-02-14 2006-03-01 三井化学株式会社 新型腈水合酶
IN209344B (de) 2000-12-20 2007-09-21 Dia Nitrix Co Ltd
JP4289151B2 (ja) 2001-07-26 2009-07-01 味の素株式会社 ジペプチドの製造方法、それに用いるｌ−アミノ酸アミドハイドロラーゼ、および、ｌ−アミノ酸アミドハイドロラーゼの製造方法
TWI332953B (en) * 2002-02-08 2010-11-11 Mitsubishi Rayon Co Method for preparing lactone
DE102004013847A1 (de) * 2004-03-20 2005-10-06 Degussa Ag Cyanidtolerante Nitrilhydratasen
JP5967189B2 (ja) * 2012-02-28 2016-08-10 三菱レイヨン株式会社 酵素の保存方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5937951B2 (ja) * 1981-11-18 1984-09-12 秀明 山田 アミドの生物学的製造法
JP2840253B2 (ja) * 1988-07-06 1998-12-24 輝彦 別府 ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体によるニトリル類からアミド類の製造法
AU627648B2 (en) * 1990-02-28 1992-08-27 Teruhiko Beppu Dna fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant

Also Published As

Publication number Publication date
CN1054614A (zh) 1991-09-18
EP0444639A2 (de) 1991-09-04
RU2028380C1 (ru) 1995-02-09
KR910021479A (ko) 1991-12-20
EP0444639A3 (en) 1992-07-08
JPH03251184A (ja) 1991-11-08
DE69116704T2 (de) 1996-08-08
AU636008B2 (en) 1993-04-08
CN1052509C (zh) 2000-05-17
JP2907479B2 (ja) 1999-06-21
KR950000888B1 (ko) 1995-02-03
RU2082761C1 (ru) 1997-06-27
EP0444639B1 (de) 1996-01-31
AU7129591A (en) 1991-09-26

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: MITSUBISHI RAYON CO., LTD., TOKIO/TOKYO, JP BEPPU,