DE69031389T2 - Schnelle, vielseitige und einfache methode zur expression von genen in eukaryotischen zellen - Google Patents
Schnelle, vielseitige und einfache methode zur expression von genen in eukaryotischen zellenInfo
- Publication number
- DE69031389T2 DE69031389T2 DE69031389T DE69031389T DE69031389T2 DE 69031389 T2 DE69031389 T2 DE 69031389T2 DE 69031389 T DE69031389 T DE 69031389T DE 69031389 T DE69031389 T DE 69031389T DE 69031389 T2 DE69031389 T2 DE 69031389T2
- Authority
- DE
- Germany
- Prior art keywords
- versatile
- eukaryotic cells
- fast
- simple method
- expressing genes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12N9/1033—Chloramphenicol O-acetyltransferase (2.3.1.28)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/24011—Poxviridae
- C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
- C12N2710/24141—Use of virus, viral particle or viral elements as a vector
- C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/32011—Picornaviridae
- C12N2770/32211—Cardiovirus, e.g. encephalomyocarditis virus
- C12N2770/32241—Use of virus, viral particle or viral elements as a vector
- C12N2770/32243—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/376,687 US5135855A (en) | 1986-09-03 | 1989-07-07 | Rapid, versatile and simple system for expressing genes in eukaryotic cells |
PCT/US1990/003687 WO1991000905A1 (en) | 1989-07-07 | 1990-07-03 | Rapid, versatile and simple system for expressing genes in eukaryotic cells |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69031389D1 DE69031389D1 (de) | 1997-10-09 |
DE69031389T2 true DE69031389T2 (de) | 1998-03-19 |
Family
ID=23486043
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69031389T Expired - Lifetime DE69031389T2 (de) | 1989-07-07 | 1990-07-03 | Schnelle, vielseitige und einfache methode zur expression von genen in eukaryotischen zellen |
Country Status (9)
Country | Link |
---|---|
US (1) | US5135855A (de) |
EP (1) | EP0484374B1 (de) |
JP (1) | JP2525289B2 (de) |
AT (1) | ATE157701T1 (de) |
AU (1) | AU642657B2 (de) |
CA (1) | CA2063427C (de) |
DE (1) | DE69031389T2 (de) |
ES (1) | ES2109924T3 (de) |
WO (1) | WO1991000905A1 (de) |
Families Citing this family (42)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0561034B1 (de) * | 1991-08-26 | 1999-06-09 | IMMUNO Aktiengesellschaft | Direkt molekuläre Klonierung eines modifizierten Genoms eines Chordopocken-Virus |
GB9125896D0 (en) * | 1991-12-05 | 1992-02-05 | Almond Jeffrey W | Bicistronic viruses |
US6221648B1 (en) | 1992-02-27 | 2001-04-24 | Microdial Technics Ltd. | Heterologous gene expression in lactococcus, and the expression products therefrom |
IL102404A0 (en) * | 1992-07-03 | 1993-01-14 | Daniel Zurr | Method and means for the production of gene products |
US5648235A (en) * | 1992-07-03 | 1997-07-15 | Q.B.I. Enterprises Ltd. | Method and means for the production of gene products, novel recombinant DNA vectors therefor and kits employing them |
US5409823A (en) * | 1992-09-24 | 1995-04-25 | Ciba-Geigy Corporation | Methods for the production of hybrid seed |
CN1120315A (zh) * | 1993-04-09 | 1996-04-10 | 维吉尼亚大学专利基金会 | 溶组织内阿米巴粘附外源凝集素的35/31千道尔顿亚基 |
US5591601A (en) * | 1993-05-14 | 1997-01-07 | Ohio University Edison Animal Biotechnology Institute | DNA polymerase gene expression system utilizing an RNA polymerase co-delivered with the gene expression vector system |
WO1995033835A1 (en) | 1994-06-02 | 1995-12-14 | Chiron Corporation | Nucleic acid immunization using a virus-based infection/transfection system |
US6242187B1 (en) * | 1996-01-29 | 2001-06-05 | Virologic, Inc. | Compositions and methods for determining anti-viral drug susceptibility and resistance and anti-viral drug screening |
US5837464A (en) * | 1996-01-29 | 1998-11-17 | Virologic, Inc. | Compositions and methods for determining anti-viral drug susceptibility and resistance and anti-viral drug screening |
US5994526A (en) * | 1996-06-21 | 1999-11-30 | Plant Genetic Systems | Gene expression in plants |
CA2255057A1 (en) * | 1996-06-21 | 1997-12-31 | Plant Genetic Systems, N.V. | Gene expression in plants |
WO1998010088A1 (en) * | 1996-09-06 | 1998-03-12 | Trustees Of The University Of Pennsylvania | An inducible method for production of recombinant adeno-associated viruses utilizing t7 polymerase |
US20020034732A1 (en) * | 1997-07-30 | 2002-03-21 | Daniel J. Capon | Compositions and methods for determining anti-viral drug susceptibility and resistance and anti-viral drug screening |
CA2305785A1 (en) * | 1997-10-07 | 1999-04-15 | University Of Maryland Biotechnology Institute | Method for introducing and expressing rna in animal cells |
WO2000039302A2 (en) | 1998-12-31 | 2000-07-06 | Chiron Corporation | Improved expression of hiv polypeptides and production of virus-like particles |
US7935805B1 (en) | 1998-12-31 | 2011-05-03 | Novartis Vaccines & Diagnostics, Inc | Polynucleotides encoding antigenic HIV Type C polypeptides, polypeptides and uses thereof |
US7575860B2 (en) * | 2000-03-07 | 2009-08-18 | Evans David H | DNA joining method |
US20020012971A1 (en) * | 2000-03-20 | 2002-01-31 | Mehta Tammy Burd | PCR compatible nucleic acid sieving medium |
GB2362383B (en) * | 2000-05-19 | 2003-12-31 | Devgen Nv | Gene expression system |
EP1363663B1 (de) | 2001-01-12 | 2011-03-02 | Novartis Vaccines and Diagnostics, Inc. | Nukleinsäure mukosale immunisierung |
EP2412242A3 (de) | 2001-07-05 | 2012-06-13 | Novartis Vaccines and Diagnostics, Inc. | Polypeptide vom HIV-Typ C codierende Polynucleotide, Polypeptide und deren Verwendung |
WO2003004657A1 (en) | 2001-07-05 | 2003-01-16 | Chiron Corporation | Polynucleotides encoding antigenic hiv type b and/or type c polypeptides, polypeptides and uses thereof |
US20100015211A1 (en) | 2004-11-01 | 2010-01-21 | Barnett Susan W | Combination Approaches For Generating Immune Responses |
JP5215865B2 (ja) | 2005-11-22 | 2013-06-19 | ノバルティス ヴァクシンズ アンド ダイアグノスティクス インコーポレイテッド | ノロウイルス抗原およびサポウイルス抗原 |
DE102006015321A1 (de) * | 2006-03-30 | 2007-10-04 | Rina-Netzwerk Rna Technologien Gmbh | Verfahren zur Genexpression |
BRPI1005670A8 (pt) * | 2009-01-05 | 2017-12-26 | Epitogenesis Inc | composições adjuvantes e processos de uso. |
CN103717235A (zh) | 2011-06-24 | 2014-04-09 | 埃皮托吉尼西斯有限公司 | 作为抗原特异性免疫调节剂的包含选择的载体、维生素、单宁和类黄酮的组合的药物组合物 |
EP3068783B1 (de) | 2013-11-15 | 2020-09-23 | The Board of Trustees of the Leland Stanford Junior University | Agonisten des hypokretin-rezeptors 2 zur verwendung zur behandlung von herzversagen |
US10550379B2 (en) | 2015-06-29 | 2020-02-04 | The Board Of Trustees Of The Leland Stanford Junior University | Degron fusion constructs and methods for controlling protein production |
CN108348593B (zh) | 2015-08-31 | 2022-08-16 | 泰克诺瓦克斯股份有限公司 | 基于人呼吸道合胞病毒(hrsv)病毒样颗粒(vlps)的疫苗 |
JP7320601B2 (ja) | 2018-09-11 | 2023-08-03 | 上▲海▼市公共▲衛▼生▲臨▼床中心 | 広域スペクトルな抗インフルエンザワクチン免疫原及びその使用 |
WO2023070072A1 (en) | 2021-10-21 | 2023-04-27 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Retroelement-generated transcription factor decoys |
WO2023081756A1 (en) | 2021-11-03 | 2023-05-11 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Precise genome editing using retrons |
WO2023141602A2 (en) | 2022-01-21 | 2023-07-27 | Renagade Therapeutics Management Inc. | Engineered retrons and methods of use |
WO2023183588A1 (en) | 2022-03-25 | 2023-09-28 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Methods of assessing engineered retron activity, and uses thereof |
WO2023183589A1 (en) | 2022-03-25 | 2023-09-28 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Rt-dna fidelity and retron genome editing |
WO2023183627A1 (en) | 2022-03-25 | 2023-09-28 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Production of reverse transcribed dna (rt-dna) using a retron reverse transcriptase from exogenous rna |
WO2024020346A2 (en) | 2022-07-18 | 2024-01-25 | Renagade Therapeutics Management Inc. | Gene editing components, systems, and methods of use |
WO2024044673A1 (en) | 2022-08-24 | 2024-02-29 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Dual cut retron editors for genomic insertions and deletions |
WO2024044723A1 (en) | 2022-08-25 | 2024-02-29 | Renagade Therapeutics Management Inc. | Engineered retrons and methods of use |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8613481D0 (en) * | 1986-06-04 | 1986-07-09 | Diatech Ltd | Translation of mrna |
US4937190A (en) * | 1987-10-15 | 1990-06-26 | Wisconsin Alumni Research Foundation | Translation enhancer |
WO1990004026A1 (en) * | 1988-10-11 | 1990-04-19 | The United States Of America, Represented By The Secretary, United States Department Of Commerce | New plasmid constructions for high level production of eukaryotic proteins |
-
1989
- 1989-07-07 US US07/376,687 patent/US5135855A/en not_active Expired - Lifetime
-
1990
- 1990-07-03 AT AT90910982T patent/ATE157701T1/de not_active IP Right Cessation
- 1990-07-03 JP JP2510227A patent/JP2525289B2/ja not_active Expired - Lifetime
- 1990-07-03 EP EP90910982A patent/EP0484374B1/de not_active Expired - Lifetime
- 1990-07-03 ES ES90910982T patent/ES2109924T3/es not_active Expired - Lifetime
- 1990-07-03 AU AU60344/90A patent/AU642657B2/en not_active Expired
- 1990-07-03 CA CA002063427A patent/CA2063427C/en not_active Expired - Lifetime
- 1990-07-03 DE DE69031389T patent/DE69031389T2/de not_active Expired - Lifetime
- 1990-07-03 WO PCT/US1990/003687 patent/WO1991000905A1/en active IP Right Grant
Also Published As
Publication number | Publication date |
---|---|
JP2525289B2 (ja) | 1996-08-14 |
US5135855A (en) | 1992-08-04 |
WO1991000905A1 (en) | 1991-01-24 |
DE69031389D1 (de) | 1997-10-09 |
CA2063427C (en) | 1999-09-21 |
EP0484374A4 (en) | 1993-05-05 |
EP0484374B1 (de) | 1997-09-03 |
ATE157701T1 (de) | 1997-09-15 |
JPH05501048A (ja) | 1993-03-04 |
AU642657B2 (en) | 1993-10-28 |
AU6034490A (en) | 1991-02-06 |
ES2109924T3 (es) | 1998-02-01 |
EP0484374A1 (de) | 1992-05-13 |
CA2063427A1 (en) | 1991-01-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |