DE69019290D1 - Synergistisches Verfahren zur Wirtszellentransformation. - Google Patents

Synergistisches Verfahren zur Wirtszellentransformation.

Info

Publication number
DE69019290D1
DE69019290D1 DE69019290T DE69019290T DE69019290D1 DE 69019290 D1 DE69019290 D1 DE 69019290D1 DE 69019290 T DE69019290 T DE 69019290T DE 69019290 T DE69019290 T DE 69019290T DE 69019290 D1 DE69019290 D1 DE 69019290D1
Authority
DE
Germany
Prior art keywords
host cell
cell transformation
synergistic process
synergistic
transformation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69019290T
Other languages
English (en)
Other versions
DE69019290T2 (de
Inventor
Joan K Stadler
Nunzio M Antonelli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Iowa Research Foundation UIRF
Iowa State University Research Foundation ISURF
Original Assignee
University of Iowa Research Foundation UIRF
Iowa State University Research Foundation ISURF
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Iowa Research Foundation UIRF, Iowa State University Research Foundation ISURF filed Critical University of Iowa Research Foundation UIRF
Publication of DE69019290D1 publication Critical patent/DE69019290D1/de
Application granted granted Critical
Publication of DE69019290T2 publication Critical patent/DE69019290T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
DE69019290T 1989-09-28 1990-09-28 Synergistisches Verfahren zur Wirtszellentransformation. Expired - Fee Related DE69019290T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/414,091 US5286634A (en) 1989-09-28 1989-09-28 Synergistic method for host cell transformation

Publications (2)

Publication Number Publication Date
DE69019290D1 true DE69019290D1 (de) 1995-06-14
DE69019290T2 DE69019290T2 (de) 1995-10-12

Family

ID=23639921

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69019290T Expired - Fee Related DE69019290T2 (de) 1989-09-28 1990-09-28 Synergistisches Verfahren zur Wirtszellentransformation.

Country Status (4)

Country Link
US (1) US5286634A (de)
EP (1) EP0424688B1 (de)
DE (1) DE69019290T2 (de)
DK (1) DK0424688T3 (de)

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WO2024031051A1 (en) 2022-08-05 2024-02-08 Life Technologies Corporation Lipids for nucleic acid delivery

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EP0424688B1 (de) 1995-05-10
EP0424688A2 (de) 1991-05-02
DE69019290T2 (de) 1995-10-12
US5286634A (en) 1994-02-15
DK0424688T3 (da) 1995-07-10
EP0424688A3 (en) 1991-07-31

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