DE69010096D1 - Expression und Sekretion von reifem menschlichem beta-Interleukin-1 in Bacillus subtilis und Mittel und Verfahren zu deren Ausführung. - Google Patents

Expression und Sekretion von reifem menschlichem beta-Interleukin-1 in Bacillus subtilis und Mittel und Verfahren zu deren Ausführung.

Info

Publication number
DE69010096D1
DE69010096D1 DE69010096T DE69010096T DE69010096D1 DE 69010096 D1 DE69010096 D1 DE 69010096D1 DE 69010096 T DE69010096 T DE 69010096T DE 69010096 T DE69010096 T DE 69010096T DE 69010096 D1 DE69010096 D1 DE 69010096D1
Authority
DE
Germany
Prior art keywords
human beta
interleukin
secretion
expression
mature human
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69010096T
Other languages
English (en)
Other versions
DE69010096T2 (de
Inventor
Bellini Ada Velati
Giuliano Galli
Giuseppe Lucchese
Guido Grandi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eni Tecnologie SpA
Original Assignee
Eniricerche SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eniricerche SpA filed Critical Eniricerche SpA
Publication of DE69010096D1 publication Critical patent/DE69010096D1/de
Application granted granted Critical
Publication of DE69010096T2 publication Critical patent/DE69010096T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/839—Bacillus subtilis
DE69010096T 1989-07-19 1990-07-13 Expression und Sekretion von reifem menschlichem beta-Interleukin-1 in Bacillus subtilis und Mittel und Verfahren zu deren Ausführung. Expired - Fee Related DE69010096T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT8921228A IT1231156B (it) 1989-07-19 1989-07-19 Espressione e secrezione di interleuchina 1 beta umana matura in bacillus subtilis e mezzi e metodi per il suo ottenimento.

Publications (2)

Publication Number Publication Date
DE69010096D1 true DE69010096D1 (de) 1994-07-28
DE69010096T2 DE69010096T2 (de) 1994-10-13

Family

ID=11178709

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69010096T Expired - Fee Related DE69010096T2 (de) 1989-07-19 1990-07-13 Expression und Sekretion von reifem menschlichem beta-Interleukin-1 in Bacillus subtilis und Mittel und Verfahren zu deren Ausführung.

Country Status (8)

Country Link
US (2) US5437988A (de)
EP (1) EP0409098B1 (de)
AT (1) ATE107701T1 (de)
CA (1) CA2021406A1 (de)
DE (1) DE69010096T2 (de)
DK (1) DK0409098T3 (de)
ES (1) ES2058695T3 (de)
IT (1) IT1231156B (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1270866B (it) * 1993-03-10 1997-05-13 Eniricerche Spa Vettore ricombinante e suo impiego per la preparazione esocellulare di anticorpi in forma di singola molecola da bacillus subtilis
US6270758B1 (en) 1998-10-08 2001-08-07 Duke University Substantially non-toxic biologically active mucosal adjuvants in vertebrate subjects
MY138219A (en) 2001-07-13 2009-05-29 Cms Peptides Patent Holding Company Ltd Biologically active peptides
US7910115B2 (en) 2003-04-30 2011-03-22 Shigekazu Nakatsugawa Polypeptide absorbable into digestive organs
TWI353252B (en) 2004-04-28 2011-12-01 Cms Peptides Patent Holding Company Ltd Biologically active peptide vapeehptllteaplnpk der
US20070061899A1 (en) * 2005-04-13 2007-03-15 The Trustees Of Columbia University In The City Of New York Transgenic mammal with constitutive, tissue-specific cytokine expression and use as a cancer model
NZ591825A (en) 2005-07-26 2012-10-26 Cms Peptides Patent Holding Company Ltd Biologically active peptides comprising PTTKTYFPHF and their uses in the manufacture of a medicament for modulating fatigue
CN103403026B (zh) 2009-09-25 2016-05-11 美国政府(由卫生和人类服务部的部长所代表) Hiv-1中和抗体及其用途
US9695230B2 (en) 2011-12-08 2017-07-04 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Broadly neutralizing HIV-1 VRC07 antibodies that bind to the CD4-binding site of the envelope protein
WO2014126921A1 (en) 2013-02-12 2014-08-21 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Monoclonal antibodies that neutralize norovirus
EP2994163B1 (de) 2013-05-09 2019-08-28 The United States of America, as represented by The Secretary, Department of Health and Human Services Single-domain-vhh-antikörper gegen norovirus gi.1 und gii.4 und deren verwendung
US10072070B2 (en) 2014-12-05 2018-09-11 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Potent anti-influenza A neuraminidase subtype N1 antibody
GB201710838D0 (en) 2017-07-05 2017-08-16 Ucl Business Plc Bispecific antibodies

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE251158C (de) *
GB2171703B (en) * 1985-01-18 1989-11-15 Agency Ind Science Techn Expression-secretion vector for gene expression and protein secretion, recombinant dna including the vector, and method of producing proteins by use of the re
IT1186750B (it) * 1985-07-10 1987-12-16 Eniricerche Spa Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni
JPH0669377B2 (ja) * 1985-11-25 1994-09-07 工業技術院長 Ｄｎａ塩基配列およびベクタ−
IT1196484B (it) * 1986-07-11 1988-11-16 Sclavo Spa Vettore ad espressione e secrezione in lieviti,utile per la preparazione di proteine eterologhe
DD251158A1 (de) * 1986-07-17 1987-11-04 Adw Ddr Verfahren zur herstellung von streptokokken-vektorplasmiden mit polylinker
IT1228925B (it) * 1987-08-07 1991-07-10 Eniricerche Spa Procedimento per la preparazione dell'ormone della crescita umano
IT1226713B (it) * 1988-08-05 1991-02-05 Eniricerche Spa Procedimento per la preparazione di interleuchina 1 beta umana ricombinante in forma omogenea.

Also Published As

Publication number Publication date
US5728571A (en) 1998-03-17
IT8921228A0 (it) 1989-07-19
ES2058695T3 (es) 1994-11-01
US5437988A (en) 1995-08-01
DK0409098T3 (da) 1994-07-25
EP0409098B1 (de) 1994-06-22
IT1231156B (it) 1991-11-19
CA2021406A1 (en) 1991-01-20
EP0409098A1 (de) 1991-01-23
ATE107701T1 (de) 1994-07-15
DE69010096T2 (de) 1994-10-13

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Legal Events

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8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee