DE69009476D1 - Hemmung des reaktionswegs für die n-ende-regel in lebenden zellen. - Google Patents

Hemmung des reaktionswegs für die n-ende-regel in lebenden zellen.

Info

Publication number
DE69009476D1
DE69009476D1 DE69009476T DE69009476T DE69009476D1 DE 69009476 D1 DE69009476 D1 DE 69009476D1 DE 69009476 T DE69009476 T DE 69009476T DE 69009476 T DE69009476 T DE 69009476T DE 69009476 D1 DE69009476 D1 DE 69009476D1
Authority
DE
Germany
Prior art keywords
amino acid
protein
terminal
type
regulator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69009476T
Other languages
English (en)
Other versions
DE69009476T2 (de
Inventor
Rohan Baker
David Gonda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
Original Assignee
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Institute of Technology filed Critical Massachusetts Institute of Technology
Publication of DE69009476D1 publication Critical patent/DE69009476D1/de
Application granted granted Critical
Publication of DE69009476T2 publication Critical patent/DE69009476T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fats And Perfumes (AREA)
DE69009476T 1989-06-30 1990-06-27 Hemmung des reaktionswegs für die n-ende-regel in lebenden zellen. Expired - Fee Related DE69009476T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37382589A 1989-06-30 1989-06-30
PCT/US1990/003669 WO1991000356A1 (en) 1989-06-30 1990-06-27 Inhibition of the n-end rule pathway in living cells

Publications (2)

Publication Number Publication Date
DE69009476D1 true DE69009476D1 (de) 1994-07-07
DE69009476T2 DE69009476T2 (de) 1994-12-22

Family

ID=23474039

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69009476T Expired - Fee Related DE69009476T2 (de) 1989-06-30 1990-06-27 Hemmung des reaktionswegs für die n-ende-regel in lebenden zellen.

Country Status (10)

Country Link
US (1) US5766927A (de)
EP (1) EP0479912B1 (de)
JP (1) JP2904921B2 (de)
AT (1) ATE106449T1 (de)
AU (1) AU5963290A (de)
CA (1) CA2063400C (de)
DE (1) DE69009476T2 (de)
DK (1) DK0479912T3 (de)
ES (1) ES2054365T3 (de)
WO (1) WO1991000356A1 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5861312A (en) * 1997-12-02 1999-01-19 California Institute Of Technology Nucleic acid encoding mammalian UBR1
AU2605600A (en) * 1999-01-11 2000-08-01 Mindset Biopharmaceuticals (Usa) Inc. Methods for regulating the stability of recombinant proteins and products usefultherein
US6306663B1 (en) 1999-02-12 2001-10-23 Proteinex, Inc. Controlling protein levels in eucaryotic organisms
WO2000050089A2 (en) * 1999-02-26 2000-08-31 Mindset Biopharmaceuticals (Usa) Ltd. Regulation of the stability of recombinant proteins and antiboodies
US7262005B1 (en) 2000-02-04 2007-08-28 Aurora Biosciences Corporation Methods of protein destabilization and uses thereof
WO2004058157A2 (en) * 2002-12-16 2004-07-15 Globeimmune, Inc. Yeast-based vaccines as immunotherapy
US8343502B2 (en) 2002-12-16 2013-01-01 Globeimmune, Inc. Yeast-based vaccines as immunotherapy
WO2008039483A2 (en) * 2006-09-26 2008-04-03 Massachusetts Institute Of Technology Modified self-assembling peptides
US8173792B2 (en) * 2007-02-09 2012-05-08 The Board Of Trustees Of The Leland Stanford Junior University Method for regulating protein function in cells using synthetic small molecules
US8530636B2 (en) * 2008-05-07 2013-09-10 The Board Of Trustees Of The Leland Stanford Junior University Method for regulating protein function in cells in vivo using synthetic small molecules
WO2011088421A2 (en) * 2010-01-15 2011-07-21 California Institute Of Technology Discovery and applications of the proteolytic function of n-terminal acetylation of cellular proteins
US11446398B2 (en) 2016-04-11 2022-09-20 Obsidian Therapeutics, Inc. Regulated biocircuit systems
WO2021216621A2 (en) 2020-04-20 2021-10-28 Vestaron Corporation Proteolytically stable u1-agatoxin-ta1b variant polypeptides for pest control
AU2021265277A1 (en) 2020-05-01 2022-12-08 Vestaron Corporation Insecticidal combinations
IL307293A (en) 2021-04-01 2023-11-01 Vestaron Corp AV3 mutant polypeptides for pest control
IL313184A (en) 2021-11-29 2024-07-01 Ironwood Pharmaceuticals Inc Medicinal preparations for the treatment of visceral pain
WO2023122805A1 (en) 2021-12-20 2023-06-29 Vestaron Corporation Sorbitol driven selection pressure method
AR129351A1 (es) 2022-05-18 2024-08-14 Vestaron Corp Variantes de péptidos pesticidas actx
WO2023245100A1 (en) 2022-06-17 2023-12-21 Vestaron Corporation Antimicrobial ncr13 variant peptides
WO2024026406A2 (en) 2022-07-29 2024-02-01 Vestaron Corporation Next Generation ACTX Peptides

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4512979A (en) * 1981-03-23 1985-04-23 Merck & Co., Inc. Dipeptides containing thialysine and related amino acids as antihypertensives
US4514391A (en) * 1983-07-21 1985-04-30 E. R. Squibb & Sons, Inc. Hydroxy substituted peptide compounds
US4551445A (en) * 1983-10-14 1985-11-05 The Medical College Of Ohio Derivatives of arginine vasopressin antagonists
US5132213A (en) * 1986-10-02 1992-07-21 Massachusetts Institute Of Technology Method for producing proteins and polypeptides using ubiquitin fusions
EP0324789B1 (de) * 1986-10-02 2003-08-20 Massachusetts Institute Of Technology Verfahren zur regulierung der metabolischen stabilität von proteinen
US5108919A (en) * 1988-06-24 1992-04-28 Genentech, Inc. Dna sequences encoding yeast ubiquitin hydrolase
CH680013A5 (de) * 1988-09-19 1992-05-29 Mathias Och

Also Published As

Publication number Publication date
CA2063400A1 (en) 1990-12-31
WO1991000356A1 (en) 1991-01-10
JPH04506298A (ja) 1992-11-05
JP2904921B2 (ja) 1999-06-14
EP0479912A1 (de) 1992-04-15
ES2054365T3 (es) 1994-08-01
DK0479912T3 (da) 1994-10-10
AU5963290A (en) 1991-01-17
US5766927A (en) 1998-06-16
DE69009476T2 (de) 1994-12-22
CA2063400C (en) 1997-07-15
EP0479912B1 (de) 1994-06-01
ATE106449T1 (de) 1994-06-15

Similar Documents

Publication Publication Date Title
DE69009476D1 (de) Hemmung des reaktionswegs für die n-ende-regel in lebenden zellen.
Giga et al. The complete amino acid sequence of echinoidin, a lectin from the coelomic fluid of the sea urchin Anthocidaris crassispina. Homologies with mammalian and insect lectins.
Reed et al. Sequence of residues 400--403 of bovine serum albumin.
Gregory et al. The primary structure of human urogastrone
Cameron et al. Amino acid sequence analysis of human interleukin 1 (IL-1). Evidence for biochemically distinct forms of IL-1.
ES2063350T3 (es) Nuevas proteinas con efecto inhibidor del tnf y su produccion.
Chorev et al. Modifications of position 12 in a parathyroid hormone and parathyroid hormone-related protein: toward the design of highly potent antagonists
FI842166A (fi) Bukspottskoertelns grf hos maenniskan.
KR850008681A (ko) 합성펩티드의 제조방법
HU215582B (hu) Eljárás humán inzulin N-terminális szekvenciájának enzimes eltávolítására
HUP0003592A2 (hu) LH-RH peptid analógok, alkalmazásuk, és ezeket tartalmazó gyógyászati készítmények
EP0451867A1 (de) Parathyroid-Hormon-Antagonisten
DE3261304D1 (en) Retro-inverso analogues of c-terminal penta and hexapeptides of substance p.
HUP0000320A2 (hu) Biológiailag aktív peptidek,és eljárás előállításukra
Ramage et al. Solid phase peptide synthesis of ubiquitin
ABEL et al. Structural differences in the hinge region of human gamma A myeloma proteins of different subclasses
DK0446315T3 (da) Fremgangsmåde til fremstilling af PAI-2
Brandt et al. Complete amino acid sequence of the N-terminal extension of calf skin type III procollagen
Hackeng et al. Chemical synthesis of human protein S thrombin‐sensitive module and first epidermal growth factor module
AU2676399A (en) Novel cystine knot protein and materials and methods for making it
NO20016341L (no) Inhibitorer for integrinet <alfa>v<beta>6
US5252705A (en) Peptide derivatives
Schwartz et al. Insulin analog possessing higher in vitro biological activity than receptor binding affinity.[21-Proline-B] insulin
Bodenmuller Isolation and structural analysis of a new neuropeptide, the head activator
KNORR et al. [B17-D-Leucine] Insulin and [B17-Norleucine] Insulin: synthesis and biological properties

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee