ES2054365T3 - Inhibicion de la via de la regla del n final en celulas vivas. - Google Patents

Inhibicion de la via de la regla del n final en celulas vivas.

Info

Publication number
ES2054365T3
ES2054365T3 ES90911196T ES90911196T ES2054365T3 ES 2054365 T3 ES2054365 T3 ES 2054365T3 ES 90911196 T ES90911196 T ES 90911196T ES 90911196 T ES90911196 T ES 90911196T ES 2054365 T3 ES2054365 T3 ES 2054365T3
Authority
ES
Spain
Prior art keywords
amino acid
protein
terminal
type
regulator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90911196T
Other languages
English (en)
Inventor
Rohan T Baker
David K Gonda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
Original Assignee
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Institute of Technology filed Critical Massachusetts Institute of Technology
Application granted granted Critical
Publication of ES2054365T3 publication Critical patent/ES2054365T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Abstract

LA INVENCION QUE SE DESCRIBE SE REFIERE A UN METODO PARA EJERCER UNA INFLUENCIA EN LA ESTABILIDAD METABOLICA DE UNA PROTEINA INTRACELULAR IN VIVO (EN CELULAS INTACTAS Y EN ANIMALES SANOS) ASI COMO A COMPOSICIONES PARA LLEVAR A CABO DICHA REGULACION. EL METODO CONSISTE EN ADMINISTRAR UN REGULADOR QUE TIENE UN RESIDUO AMINOACIDO DE TERMINAL AMINO QUE ES IGUAL O PARECIDO AL RESIDUO TERMINAL AMINO DE LA PROTEINA INTRACELULAR O GRUPO DE PROTEINAS. SI SE DESEA, ESTE DESEA, ESTE RESIDUO TERMINAL AMINO SE ELIGE COMO MIEMBRO DE LA CLASE DESESTABILIZADORA DE RESIDUOS DE TERMINAL AMINO, SEGUNA LA TRAZA FINAL DE N DE LA DEGRADACION PROTEINICA. LAS COMPOSICIONES DE LA INVENCION PUEDEN CONTENER UNA FORMACION DE ADN QUE CODIFICA UNA PROTEINA Y UNA FORMACION DE ADN QUE CODIFICA EL MISMO RESIDUO, O PARECIDO, TERMINAL AMINO QUE LA PROTEINA. LAS COMPOSICIONES PUEDEN CONTENER TAMBIEN EL REGULADOR, QUE TIENE UN RESIDUO TERMINAL AMINO QUE ES IGUAL O SIMILAR AL RESIDUO TERMINAL AMINO DE LA PROTEINA Y, COMO SE HADESEADO, ES UN MIEMBRO DE LA CLASE DESESTABILIZADORA DE LOS RESIDUOS DE TERMINAL AMINO, SEGUN LA TRAZA FINAL DE N DE LA DEGRADACION PROTEINICA. LOS METODOS Y COMPOSICIONES DE LA INVENCION SE PUEDEN UTILIZAR PARA EL TRATAMIENTO DE ENFERMEDADES OCASIONADAS POR LA ROTURA ANORMAL DE UNA PROTEINA DESEADA O GRUPO DE PROTEINAS Y PARA AUMENTAR LA PRODUCCION IN VIVO DE UNA PROTEINA DESEADA O GRUPO DE PROTEINAS EN APLICACIONES BIOTECNOLOGICAS.
ES90911196T 1989-06-30 1990-06-27 Inhibicion de la via de la regla del n final en celulas vivas. Expired - Lifetime ES2054365T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US37382589A 1989-06-30 1989-06-30

Publications (1)

Publication Number Publication Date
ES2054365T3 true ES2054365T3 (es) 1994-08-01

Family

ID=23474039

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90911196T Expired - Lifetime ES2054365T3 (es) 1989-06-30 1990-06-27 Inhibicion de la via de la regla del n final en celulas vivas.

Country Status (10)

Country Link
US (1) US5766927A (es)
EP (1) EP0479912B1 (es)
JP (1) JP2904921B2 (es)
AT (1) ATE106449T1 (es)
AU (1) AU5963290A (es)
CA (1) CA2063400C (es)
DE (1) DE69009476T2 (es)
DK (1) DK0479912T3 (es)
ES (1) ES2054365T3 (es)
WO (1) WO1991000356A1 (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5861312A (en) * 1997-12-02 1999-01-19 California Institute Of Technology Nucleic acid encoding mammalian UBR1
AU2605600A (en) * 1999-01-11 2000-08-01 Mindset Biopharmaceuticals (Usa) Inc. Methods for regulating the stability of recombinant proteins and products usefultherein
US6306663B1 (en) * 1999-02-12 2001-10-23 Proteinex, Inc. Controlling protein levels in eucaryotic organisms
AU3604800A (en) * 1999-02-26 2000-09-14 Mindset Biopharmaceuticals (Usa) Ltd. Method for regulating the stability of recombinant proteins, and antibodies and products useful therein
US7262005B1 (en) 2000-02-04 2007-08-28 Aurora Biosciences Corporation Methods of protein destabilization and uses thereof
JP4914990B2 (ja) 2002-12-16 2012-04-11 グローブイミューン，インコーポレイテッド 動物を癌から守るための治療用組成物
US8343502B2 (en) 2002-12-16 2013-01-01 Globeimmune, Inc. Yeast-based vaccines as immunotherapy
ES2709125T3 (es) * 2006-09-26 2019-04-15 Massachusetts Inst Technology Péptidos autoensamblantes modificados
US8173792B2 (en) 2007-02-09 2012-05-08 The Board Of Trustees Of The Leland Stanford Junior University Method for regulating protein function in cells using synthetic small molecules
US8530636B2 (en) 2008-05-07 2013-09-10 The Board Of Trustees Of The Leland Stanford Junior University Method for regulating protein function in cells in vivo using synthetic small molecules
WO2011088421A2 (en) * 2010-01-15 2011-07-21 California Institute Of Technology Discovery and applications of the proteolytic function of n-terminal acetylation of cellular proteins
EP3443001A4 (en) 2016-04-11 2020-04-29 Obsidian Therapeutics, Inc. REGULATED BIOCIRCUIT SYSTEMS
CN116018350A (zh) 2020-04-20 2023-04-25 韦斯塔隆公司 用于有害生物防治的蛋白水解稳定的U1-漏斗网蛛毒素-Ta1b变体多肽
IL297738A (en) 2020-05-01 2022-12-01 Vestaron Corp Insecticide combinations
AU2022249371A1 (en) 2021-04-01 2023-10-26 Vestaron Corporation Av3 mutant polypeptides for pest control
WO2023097309A1 (en) 2021-11-29 2023-06-01 Ironwood Pharmaceuticals, Inc. Pharmaceutical compositions for the treatment of visceral pain
WO2023122805A1 (en) 2021-12-20 2023-06-29 Vestaron Corporation Sorbitol driven selection pressure method
WO2023225555A1 (en) 2022-05-18 2023-11-23 Vestaron Corporation Pesticidal actx peptide variants
WO2023245100A1 (en) 2022-06-17 2023-12-21 Vestaron Corporation Antimicrobial ncr13 variant peptides
WO2024026406A2 (en) 2022-07-29 2024-02-01 Vestaron Corporation Next Generation ACTX Peptides

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4512979A (en) * 1981-03-23 1985-04-23 Merck & Co., Inc. Dipeptides containing thialysine and related amino acids as antihypertensives
US4514391A (en) * 1983-07-21 1985-04-30 E. R. Squibb & Sons, Inc. Hydroxy substituted peptide compounds
US4551445A (en) * 1983-10-14 1985-11-05 The Medical College Of Ohio Derivatives of arginine vasopressin antagonists
EP0324789B1 (en) * 1986-10-02 2003-08-20 Massachusetts Institute Of Technology Methods of regulating metabolic stability of proteins
US5132213A (en) * 1986-10-02 1992-07-21 Massachusetts Institute Of Technology Method for producing proteins and polypeptides using ubiquitin fusions
US5108919A (en) * 1988-06-24 1992-04-28 Genentech, Inc. Dna sequences encoding yeast ubiquitin hydrolase
CH680013A5 (es) * 1988-09-19 1992-05-29 Mathias Och

Also Published As

Publication number Publication date
JPH04506298A (ja) 1992-11-05
DK0479912T3 (da) 1994-10-10
CA2063400A1 (en) 1990-12-31
US5766927A (en) 1998-06-16
EP0479912B1 (en) 1994-06-01
ATE106449T1 (de) 1994-06-15
CA2063400C (en) 1997-07-15
DE69009476T2 (de) 1994-12-22
DE69009476D1 (de) 1994-07-07
EP0479912A1 (en) 1992-04-15
AU5963290A (en) 1991-01-17
WO1991000356A1 (en) 1991-01-10
JP2904921B2 (ja) 1999-06-14

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