DE60332774D1 - Verbesserte sso7-polymerase-konjugat-proteine - Google Patents
Verbesserte sso7-polymerase-konjugat-proteineInfo
- Publication number
- DE60332774D1 DE60332774D1 DE60332774T DE60332774T DE60332774D1 DE 60332774 D1 DE60332774 D1 DE 60332774D1 DE 60332774 T DE60332774 T DE 60332774T DE 60332774 T DE60332774 T DE 60332774T DE 60332774 D1 DE60332774 D1 DE 60332774D1
- Authority
- DE
- Germany
- Prior art keywords
- improved
- polymerase conjugate
- conjugate proteins
- sso7 polymerase
- sso7
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/280,139 US7666645B2 (en) | 2002-10-23 | 2002-10-23 | Sso7-polymerase conjugate proteins |
| PCT/US2003/032954 WO2004037979A2 (en) | 2002-10-23 | 2003-10-20 | Improved sso7-polymerase conjugate proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60332774D1 true DE60332774D1 (de) | 2010-07-08 |
Family
ID=32106848
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60332774T Expired - Lifetime DE60332774D1 (de) | 2002-10-23 | 2003-10-20 | Verbesserte sso7-polymerase-konjugat-proteine |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US7666645B2 (de) |
| EP (1) | EP1660650B1 (de) |
| JP (2) | JP4505331B2 (de) |
| CN (2) | CN101875924A (de) |
| AT (1) | ATE469211T1 (de) |
| AU (1) | AU2003284265B2 (de) |
| CA (1) | CA2502335C (de) |
| DE (1) | DE60332774D1 (de) |
| DK (1) | DK1660650T3 (de) |
| ES (1) | ES2344892T3 (de) |
| WO (1) | WO2004037979A2 (de) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7560260B2 (en) | 2002-07-25 | 2009-07-14 | Bio-Rad Laboratories, Inc. | Compositions with polymerase activity |
| US20060105348A1 (en) * | 2004-11-15 | 2006-05-18 | Lee Jun E | Compositions and methods for the detection and discrimination of nucleic acids |
| US20060275792A1 (en) * | 2004-11-15 | 2006-12-07 | Lee Jun E | Enhancement of nucleic acid amplification using double-stranded DNA binding proteins |
| JP5241493B2 (ja) * | 2005-07-15 | 2013-07-17 | アジレント・テクノロジーズ・インク | Ｄｎａ結合タンパク質−ポリメラーゼのキメラ |
| JP5051423B2 (ja) | 2006-07-26 | 2012-10-17 | アイシン精機株式会社 | 改変型の耐熱性ＲｅｃＡタンパク質、及び該タンパク質を用いた核酸増幅方法 |
| EP1930342B1 (de) | 2006-12-04 | 2012-01-25 | Institut Pasteur | Als Gerüst verwendeter OB-Fold zur Entwicklung neuer spezifischer Bindemittel |
| US8338094B2 (en) * | 2007-11-27 | 2012-12-25 | Bio-Rad Laboratories, Inc. | Reduced inhibition of one-step RT-PCR |
| GB0803628D0 (en) | 2008-02-28 | 2008-04-02 | Genesys Ltd | Enzyme |
| JP5809059B2 (ja) | 2008-11-03 | 2015-11-10 | カパ バイオシステムズ， インコーポレイテッド | 改変ａ型ｄｎａポリメラーゼ |
| US8859205B2 (en) | 2009-01-08 | 2014-10-14 | Bio-Rad Laboratories, Inc. | Methods and compositions for improving efficiency of nucleic acids amplification reactions |
| US9778188B2 (en) | 2009-03-11 | 2017-10-03 | Industrial Technology Research Institute | Apparatus and method for detection and discrimination molecular object |
| WO2010117901A1 (en) * | 2009-04-10 | 2010-10-14 | Bio-Rad Laboratories, Inc. | Modified dnase compositions and methods of use thereof |
| WO2011059802A1 (en) * | 2009-10-29 | 2011-05-19 | Bio-Rad Laboratories, Inc. | Rapid detection of mycoplasma contamination in cell culture samples |
| US9482615B2 (en) | 2010-03-15 | 2016-11-01 | Industrial Technology Research Institute | Single-molecule detection system and methods |
| US9670243B2 (en) | 2010-06-02 | 2017-06-06 | Industrial Technology Research Institute | Compositions and methods for sequencing nucleic acids |
| US8865078B2 (en) | 2010-06-11 | 2014-10-21 | Industrial Technology Research Institute | Apparatus for single-molecule detection |
| WO2012097318A2 (en) | 2011-01-14 | 2012-07-19 | Kap Abiosystems | Modified dna polymerases for improved amplification |
| US8697410B2 (en) | 2011-04-08 | 2014-04-15 | Bio-Rad Laboratories, Inc. | SSO7-polymerase conjugates with decreased non-specific activity |
| CN103703141B (zh) * | 2011-04-08 | 2016-08-17 | 伯乐实验室公司 | 具有降低的非特异性活性的pcr反应混合物 |
| US8715987B2 (en) | 2011-05-02 | 2014-05-06 | New England Biolabs, Inc. | Solubilized phospholipids for stabilizing nucleic acid polymerases |
| US20130260422A1 (en) * | 2011-09-01 | 2013-10-03 | New England Biolabs, Inc. | Compositions and Methods Relating to Variant DNA Polymerases and Synthetic DNA Polymerases |
| US10683537B2 (en) * | 2012-10-16 | 2020-06-16 | Dna Polymerase Technology, Inc. | Inhibition-resistant polymerases |
| CN104630205B (zh) * | 2015-01-26 | 2018-01-16 | 江南大学 | 一种增强DNA聚合酶Dbh持续合成DNA能力的方法 |
| CN104560909B (zh) * | 2015-01-26 | 2017-09-29 | 江南大学 | 一种催化dna合成效率提高的dna聚合酶 |
| CN107250382A (zh) | 2015-02-17 | 2017-10-13 | 生物辐射实验室股份有限公司 | 使用分裂循环扩增的小核酸定量 |
| WO2016183294A1 (en) | 2015-05-12 | 2016-11-17 | Dna Polymerase Technology, Inc. | Mutant polymerases and uses thereof |
| WO2017167811A1 (en) | 2016-03-31 | 2017-10-05 | Genia Technologies, Inc. | Nanopore protein conjugates and uses thereof |
| EP3825412B1 (de) * | 2016-06-24 | 2023-08-30 | The Regents of The University of California | Nukleinsäuresynthese und -sequenzierung mit gebundenen nukleosidtriphosphaten |
| CN107475216B (zh) * | 2017-08-15 | 2022-06-14 | 北京擎科生物科技有限公司 | 重组型耐热dna聚合酶及其应用 |
| EP3483180A1 (de) * | 2017-11-14 | 2019-05-15 | Affilogic | Multispezifische moleküle |
| CN109988817B (zh) * | 2017-12-30 | 2020-12-04 | 安诺优达基因科技(北京)有限公司 | 一种随机打断dna的方法 |
| US11604186B2 (en) | 2018-10-17 | 2023-03-14 | Molecular Devices (Austria) GmbH | Real time western blot assays utilizing fluorescence resonance energy transfer (FRET) |
| CN111826366B (zh) * | 2020-06-05 | 2022-08-02 | 广州英赞生物科技有限公司 | 一种直扩型热启动dna聚合酶及其制备方法与应用 |
| US11667968B2 (en) | 2021-05-27 | 2023-06-06 | New England Biolabs, Inc. | Fragmentation of DNA |
| CN113604450B (zh) * | 2021-08-18 | 2023-08-18 | 华南理工大学 | 一种kod dna聚合酶突变体及其制备方法与应用 |
| WO2023146243A1 (ko) * | 2022-01-26 | 2023-08-03 | 주식회사 씨젠 | 샘플 내 타겟 핵산을 검출하는 방법 |
| WO2023232075A1 (zh) * | 2022-06-02 | 2023-12-07 | 上海吉量医药工程有限公司 | 一种rna聚合酶融合蛋白及其应用 |
| CN116218953A (zh) * | 2023-01-03 | 2023-06-06 | 南京诺唯赞生物科技股份有限公司 | 一种连接核酸片段与接头的方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5972603A (en) | 1996-02-09 | 1999-10-26 | President And Fellows Of Harvard College | DNA polymerase with modified processivity |
| US6228628B1 (en) | 1997-07-09 | 2001-05-08 | Roche Molecular Systems | Mutant chimeric DNA polymerase |
| US6627424B1 (en) | 2000-05-26 | 2003-09-30 | Mj Bioworks, Inc. | Nucleic acid modifying enzymes |
-
2002
- 2002-10-23 US US10/280,139 patent/US7666645B2/en not_active Expired - Lifetime
-
2003
- 2003-10-20 AU AU2003284265A patent/AU2003284265B2/en not_active Ceased
- 2003-10-20 CN CN2010101571257A patent/CN101875924A/zh active Pending
- 2003-10-20 CN CN200380105035.1A patent/CN1720324B/zh not_active Expired - Lifetime
- 2003-10-20 AT AT03776445T patent/ATE469211T1/de active
- 2003-10-20 EP EP03776445A patent/EP1660650B1/de not_active Expired - Lifetime
- 2003-10-20 ES ES03776445T patent/ES2344892T3/es not_active Expired - Lifetime
- 2003-10-20 WO PCT/US2003/032954 patent/WO2004037979A2/en active Application Filing
- 2003-10-20 DK DK03776445.3T patent/DK1660650T3/da active
- 2003-10-20 CA CA2502335A patent/CA2502335C/en not_active Expired - Lifetime
- 2003-10-20 DE DE60332774T patent/DE60332774D1/de not_active Expired - Lifetime
- 2003-10-20 JP JP2004546895A patent/JP4505331B2/ja not_active Expired - Lifetime
-
2010
- 2010-02-12 US US12/705,438 patent/US8445249B2/en not_active Expired - Lifetime
- 2010-02-26 JP JP2010041133A patent/JP2010166918A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CA2502335C (en) | 2012-09-04 |
| AU2003284265A1 (en) | 2004-05-13 |
| ATE469211T1 (de) | 2010-06-15 |
| DK1660650T3 (da) | 2010-09-06 |
| CA2502335A1 (en) | 2004-05-06 |
| EP1660650A2 (de) | 2006-05-31 |
| EP1660650B1 (de) | 2010-05-26 |
| WO2004037979A3 (en) | 2005-05-06 |
| CN1720324A (zh) | 2006-01-11 |
| US20100279360A1 (en) | 2010-11-04 |
| AU2003284265B2 (en) | 2008-08-28 |
| US7666645B2 (en) | 2010-02-23 |
| WO2004037979A2 (en) | 2004-05-06 |
| US20040081963A1 (en) | 2004-04-29 |
| JP2010166918A (ja) | 2010-08-05 |
| ES2344892T3 (es) | 2010-09-09 |
| JP2006503580A (ja) | 2006-02-02 |
| CN1720324B (zh) | 2010-05-26 |
| CN101875924A (zh) | 2010-11-03 |
| EP1660650A4 (de) | 2007-07-04 |
| JP4505331B2 (ja) | 2010-07-21 |
| US8445249B2 (en) | 2013-05-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |