DE60236145D1 - Zellisolierungsverfahren und verwendungen davon - Google Patents
Zellisolierungsverfahren und verwendungen davonInfo
- Publication number
- DE60236145D1 DE60236145D1 DE60236145T DE60236145T DE60236145D1 DE 60236145 D1 DE60236145 D1 DE 60236145D1 DE 60236145 T DE60236145 T DE 60236145T DE 60236145 T DE60236145 T DE 60236145T DE 60236145 D1 DE60236145 D1 DE 60236145D1
- Authority
- DE
- Germany
- Prior art keywords
- cells
- separating
- differentially stained
- property
- stained cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 4
- 238000002955 isolation Methods 0.000 title abstract 3
- 238000004720 dielectrophoresis Methods 0.000 abstract 2
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 238000000432 density-gradient centrifugation Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 238000010186 staining Methods 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Electrostatic Separation (AREA)
- Centrifugal Separators (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Element Separation (AREA)
- Local Oxidation Of Silicon (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB01110015XA CN100494360C (zh) | 2001-03-22 | 2001-03-22 | 细胞分离方法及其应用 |
PCT/US2002/008880 WO2002077269A1 (en) | 2001-03-22 | 2002-03-20 | Cell isolation method and uses thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60236145D1 true DE60236145D1 (de) | 2010-06-10 |
Family
ID=4658284
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60236145T Expired - Lifetime DE60236145D1 (de) | 2001-03-22 | 2002-03-20 | Zellisolierungsverfahren und verwendungen davon |
Country Status (8)
Country | Link |
---|---|
US (2) | US7153648B2 (de) |
EP (2) | EP2116609B1 (de) |
JP (2) | JP2004522452A (de) |
CN (1) | CN100494360C (de) |
AT (1) | ATE466096T1 (de) |
CA (1) | CA2440385C (de) |
DE (1) | DE60236145D1 (de) |
WO (1) | WO2002077269A1 (de) |
Families Citing this family (62)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8980568B2 (en) | 2001-10-11 | 2015-03-17 | Aviva Biosciences Corporation | Methods and compositions for detecting non-hematopoietic cells from a blood sample |
US8986944B2 (en) | 2001-10-11 | 2015-03-24 | Aviva Biosciences Corporation | Methods and compositions for separating rare cells from fluid samples |
US7998699B2 (en) | 2002-08-15 | 2011-08-16 | University Of South Florida | Early detection of pathogens in blood |
JP4662932B2 (ja) | 2003-08-15 | 2011-03-30 | ユニバーシティー オブ サウス フロリダ | 試料からの病原体の捕獲およびアウリントリカルボン酸除去のための物質および方法 |
US7384791B2 (en) * | 2004-01-21 | 2008-06-10 | Hewlett-Packard Development Company, L.P. | Method of analyzing blood |
US7390387B2 (en) * | 2004-03-25 | 2008-06-24 | Hewlett-Packard Development Company, L.P. | Method of sorting cells in series |
US7160425B2 (en) * | 2004-03-25 | 2007-01-09 | Hewlett-Packard Development Company, L.P. | Cell transporter for a biodevice |
US7390388B2 (en) * | 2004-03-25 | 2008-06-24 | Hewlett-Packard Development Company, L.P. | Method of sorting cells on a biodevice |
FR2876045B1 (fr) * | 2004-10-04 | 2006-11-10 | Commissariat Energie Atomique | Dispositif pour realiser la separation dielectrophoretique de particules contenues dans un fluide |
US20060177815A1 (en) * | 2004-11-29 | 2006-08-10 | The Regents Of The University Of California | Dielectrophoretic particle sorter |
KR100738071B1 (ko) * | 2005-01-21 | 2007-07-12 | 삼성전자주식회사 | 농도구배발생부가 구비된 유전영동 장치, 그를 이용한물질의 분리방법 및 물질 분리의 최적 조건을 탐색하는 방법 |
JP2009014342A (ja) * | 2005-10-19 | 2009-01-22 | Sharp Corp | 誘電泳動チップおよび誘電泳動装置並びに誘電泳動システム |
WO2007046484A1 (ja) * | 2005-10-19 | 2007-04-26 | Sharp Kabushiki Kaisha | 誘電泳動チップおよび誘電泳動装置並びに誘電泳動システム |
EP1986001A4 (de) | 2006-02-10 | 2013-11-13 | Univ Kochi Technology | Eigenschaftsanalysevorrichtung und verfahren mit nutzung der dielektrischen wanderung einer granulären substanz mittels winkelmodulierter welle |
WO2008008515A2 (en) | 2006-07-14 | 2008-01-17 | Aviva Biosciences Corporation | Methods and compositions for detecting rare cells from a biological sample |
CN101177673B (zh) * | 2006-11-06 | 2010-05-12 | 瑞鼎科技股份有限公司 | 排列细胞的方法及其使用的电极图案 |
ITTO20070307A1 (it) * | 2007-05-04 | 2008-11-05 | Silicon Biosystems Spa | Metodo e dispositivo per la diagnosi prenatale non-invasiva |
JP5120968B2 (ja) | 2007-10-05 | 2013-01-16 | 国立大学法人九州工業大学 | 誘電泳動装置および方法 |
US8162149B1 (en) | 2009-01-21 | 2012-04-24 | Sandia Corporation | Particle sorter comprising a fluid displacer in a closed-loop fluid circuit |
US9134221B2 (en) | 2009-03-10 | 2015-09-15 | The Regents Of The University Of California | Fluidic flow cytometry devices and particle sensing based on signal-encoding |
US20120251411A1 (en) | 2009-12-07 | 2012-10-04 | Min-Yong Jeon | Centrifuge tube |
JP5611582B2 (ja) * | 2009-12-25 | 2014-10-22 | 株式会社東芝 | 電気的中性物質の分離方法、及び電気的中性物質の分離装置 |
CA2787135C (en) | 2010-01-21 | 2018-11-20 | Biocep Ltd. | Magnetic separation of rare cells |
US8774488B2 (en) | 2010-03-11 | 2014-07-08 | Cellscape Corporation | Method and device for identification of nucleated red blood cells from a maternal blood sample |
US20110225809A1 (en) * | 2010-03-17 | 2011-09-22 | Alan Francis Daher | Apparatus for removably attaching an item to a surface |
JP5771917B2 (ja) * | 2010-08-04 | 2015-09-02 | 公益財団法人ヒューマンサイエンス振興財団 | 単核球分離管及び単核球分離システム |
US10024819B2 (en) | 2010-10-21 | 2018-07-17 | The Regents Of The University Of California | Microfluidics with wirelessly powered electronic circuits |
KR101284725B1 (ko) * | 2011-07-22 | 2013-07-17 | 한국항공대학교산학협력단 | 유전영동력을 이용한 고속 입자분리 시스템 |
JP2014531199A (ja) * | 2011-08-19 | 2014-11-27 | ウー アラン ヤンWU, Allan, Yang | 並行光線療法を伴う傾斜アレイ誘電泳動分離(GrADS) |
WO2013027611A1 (ja) | 2011-08-24 | 2013-02-28 | 栄研化学株式会社 | 白血球測定デバイス及び試薬キット |
WO2013036707A1 (en) | 2011-09-09 | 2013-03-14 | Spine Wave, Inc. | Lateral approach expandable spinal implant and method |
US11198126B2 (en) | 2011-10-31 | 2021-12-14 | Fluid-Screen, Inc. | Apparatus for pathogen detection |
US9120105B2 (en) | 2011-10-31 | 2015-09-01 | Monika Weber | Electronic device for pathogen detection |
US8926816B2 (en) | 2011-11-08 | 2015-01-06 | Rarecyte, Inc. | Systems and methods to analyze materials of a suspension by means of dielectrophoresis |
TWI448678B (zh) * | 2012-03-23 | 2014-08-11 | Univ Nat Cheng Kung | 液體樣本之帶電粒子的分離方法及裝置與該裝置的製作方法 |
WO2013177560A1 (en) | 2012-05-25 | 2013-11-28 | The Regents Of The University Of California | Microfluidic systems for particle trapping and separation |
TW201413230A (zh) * | 2012-09-21 | 2014-04-01 | Nat Applied Res Laboratories | 可選擇地濃縮分離待測粒子的方法與晶片 |
JP5990440B2 (ja) * | 2012-09-26 | 2016-09-14 | テルモ株式会社 | ヘマトクリット値の測定方法 |
US10816550B2 (en) | 2012-10-15 | 2020-10-27 | Nanocellect Biomedical, Inc. | Systems, apparatus, and methods for sorting particles |
KR20150133774A (ko) * | 2013-03-15 | 2015-11-30 | 테라노스, 인코포레이티드 | 시료 수집 및 시료 분리용 방법과 기기 |
US20160209299A1 (en) * | 2013-08-29 | 2016-07-21 | Apocell, Inc. | Method and apparatus for isolation, capture and molecular analysis of target particles |
WO2016045022A1 (zh) * | 2014-09-25 | 2016-03-31 | 深圳华大基因科技有限公司 | 用于孕妇外周血样的保存液和孕妇外周血样的保存方法 |
CN106513179B (zh) * | 2015-09-15 | 2019-05-31 | 王冰 | 一种钣金介电电泳电极结构 |
US10495580B2 (en) * | 2015-10-07 | 2019-12-03 | Afi Corporation | Inspection device, inspection system, and inspection method |
US9862941B2 (en) | 2015-10-14 | 2018-01-09 | Pioneer Hi-Bred International, Inc. | Single cell microfluidic device |
US10549277B2 (en) | 2015-10-14 | 2020-02-04 | The Regents Of The University Of California | Integrated microfluidic platform for selective extraction of single-cell mRNA |
EP3442711B1 (de) | 2016-04-15 | 2020-12-09 | Fluid-Screen, Inc. | Analytnachweisverfahren und -vorrichtungen mit dielektrophorese und elektroosmose |
US20180093271A1 (en) * | 2016-09-30 | 2018-04-05 | The University Of Tokyo | Microdevice for Capturing Particles, and Method for Capturing, Concentrating, or Separating Particles Using the Same |
EP3525933A1 (de) | 2016-10-11 | 2019-08-21 | The Regents of the University of California | Systeme und verfahren zur verkapselung und konservierung von organischem material zur analyse |
WO2018122852A1 (en) * | 2016-12-29 | 2018-07-05 | Schnell Amit | Cartridge for use in in-vitro diagnostics and method of use thereof |
WO2018227210A1 (en) | 2017-06-09 | 2018-12-13 | The Regents Of The University Of California | High-efficiency encapsulation in droplets based on hydrodynamic vortices control |
US11517901B2 (en) | 2017-06-09 | 2022-12-06 | The Regents Of The University Of California | High-efficiency particle encapsulation in droplets with particle spacing and downstream droplet sorting |
WO2019075409A1 (en) | 2017-10-12 | 2019-04-18 | The Regents Of The University Of California | ISOLATION AND IDENTIFICATION WITHOUT MICROFLUIDIC LABEL OF CELLS USING FLUORESCENCE LIFE IMAGING (FLIM) |
US11499127B2 (en) | 2017-10-20 | 2022-11-15 | The Regents Of The University Of California | Multi-layered microfluidic systems for in vitro large-scale perfused capillary networks |
WO2019079787A1 (en) | 2017-10-20 | 2019-04-25 | The Regents Of The University Of California | MICROFLUIDIC SYSTEMS AND METHODS FOR CELL TRANSFECTION VIA LIPOFLEX |
US11554377B2 (en) | 2018-01-29 | 2023-01-17 | Hewlett-Packard Development Company, L.P. | Object separating |
CA3161454A1 (en) | 2019-11-13 | 2021-05-20 | Fluid-Screen, Inc. | Methods and apparatus for detection of bacteria in a sample using dielectrophoresis |
EP4058055A4 (de) | 2019-11-13 | 2023-12-13 | Fluid-Screen, Inc. | Vorrichtung und verfahren zur schnellen detektion, trennung, reinigung und quantifizierung verschiedener viren aus zellen, kulturmedien und anderen flüssigkeiten |
CN110777060A (zh) * | 2019-11-14 | 2020-02-11 | 北京酷搏科技有限公司 | 反应管、反应管阵列、控制参与反应的样品体积的方法及其应用 |
CN112362711B (zh) * | 2020-11-11 | 2021-08-17 | 重庆大学 | 一种微生物检测装置及检测方法 |
CN112871229B (zh) * | 2021-01-21 | 2022-06-28 | 中国科学技术大学 | 一种用于水体介电泳细菌分选的芯片 |
WO2024036174A1 (en) * | 2022-08-08 | 2024-02-15 | Sigil Biosciences, Inc. | Systems and methods for isolating particles in solution by particle permittivity |
Family Cites Families (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3975156A (en) * | 1975-11-10 | 1976-08-17 | Ortho Diagnostics, Inc. | Method and material for detecting and quantitating fetal erythrocytes in adults |
US4326934A (en) * | 1979-12-31 | 1982-04-27 | Pohl Herbert A | Continuous dielectrophoretic cell classification method |
US4786387A (en) * | 1986-09-25 | 1988-11-22 | Whitlock David R | Single phase enrichment of super critical fluids |
US5641628A (en) * | 1989-11-13 | 1997-06-24 | Children's Medical Center Corporation | Non-invasive method for isolation and detection of fetal DNA |
GB9301122D0 (en) * | 1993-01-21 | 1993-03-10 | Scient Generics Ltd | Method of analysis/separation |
GB9306729D0 (en) * | 1993-03-31 | 1993-05-26 | British Tech Group | Improvements in separators |
US6129828A (en) * | 1996-09-06 | 2000-10-10 | Nanogen, Inc. | Apparatus and methods for active biological sample preparation |
US6071394A (en) * | 1996-09-06 | 2000-06-06 | Nanogen, Inc. | Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis |
US5662813A (en) | 1994-10-21 | 1997-09-02 | Bioseparations, Inc. | Method for separation of nucleated fetal erythrocytes from maternal blood samples |
DE19603043C2 (de) * | 1996-01-29 | 1997-11-27 | Ibm | Ionenerzeuger für ionographischen Druckkopf und Verfahren zu dessen Herstellung |
CA2245514C (en) * | 1996-01-31 | 2006-05-23 | Board Of Regents, The University Of Texas System | Fractionation using dielectrophoresis and field flow fractionation |
US5993630A (en) * | 1996-01-31 | 1999-11-30 | Board Of Regents The University Of Texas System | Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation |
US5888370A (en) * | 1996-02-23 | 1999-03-30 | Board Of Regents, The University Of Texas System | Method and apparatus for fractionation using generalized dielectrophoresis and field flow fractionation |
US5866071A (en) * | 1996-03-06 | 1999-02-02 | National Science Council | Centrifuge tube with a built-in small tubing for separation following density gradients centrifugation |
AU4113297A (en) * | 1996-09-04 | 1998-03-26 | Technical University Of Denmark | A micro flow system for particle separation and analysis |
DE19815882A1 (de) | 1998-04-08 | 1999-10-14 | Fuhr Guenther | Verfahren und Vorrichtung zur Manipulierung von Mikropartikeln in Fluidströmungen |
WO1999061888A2 (en) * | 1998-05-22 | 1999-12-02 | California Institute Of Technology | Microfabricated cell sorter |
WO1999062622A1 (en) * | 1998-05-29 | 1999-12-09 | Industrial Research Limited | Method and apparatus for concentrating and/or positioning particles or cells |
JP2002008115A (ja) * | 2000-06-23 | 2002-01-11 | Sony Corp | 情報配信システム、端末装置、サーバ装置、記録媒体、情報配信方法 |
WO2002016647A1 (en) | 2000-08-24 | 2002-02-28 | Aviva Biosciences Corporation | Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization |
CN1325909C (zh) * | 2000-09-27 | 2007-07-11 | 清华大学 | 用于微粒操纵与微粒导向的装置及其使用方法 |
AU2002211838A1 (en) * | 2000-09-27 | 2002-04-08 | Aviva Biosciences Corporation | Apparatus for switchng and manipulating particles and method of use thereof |
AU2002213426A1 (en) | 2000-09-30 | 2002-04-15 | Aviva Biosciences Corporation | Apparatuses and methods for field flow fractionation of particles using acoustic and other forces |
CN100495030C (zh) * | 2000-09-30 | 2009-06-03 | 清华大学 | 多力操纵装置及其应用 |
AU2001292997A1 (en) | 2000-09-30 | 2002-04-15 | Aviva Biosciences Corporation | Apparatuses containing multiple force generating elements and uses thereof |
-
2001
- 2001-03-22 CN CNB01110015XA patent/CN100494360C/zh not_active Expired - Fee Related
-
2002
- 2002-03-20 EP EP09007354.5A patent/EP2116609B1/de not_active Expired - Lifetime
- 2002-03-20 AT AT02728540T patent/ATE466096T1/de not_active IP Right Cessation
- 2002-03-20 EP EP02728540A patent/EP1379682B1/de not_active Expired - Lifetime
- 2002-03-20 JP JP2002575311A patent/JP2004522452A/ja active Pending
- 2002-03-20 DE DE60236145T patent/DE60236145D1/de not_active Expired - Lifetime
- 2002-03-20 US US10/103,581 patent/US7153648B2/en not_active Expired - Lifetime
- 2002-03-20 CA CA2440385A patent/CA2440385C/en not_active Expired - Fee Related
- 2002-03-20 WO PCT/US2002/008880 patent/WO2002077269A1/en active IP Right Grant
-
2005
- 2005-10-25 JP JP2005310561A patent/JP4411266B2/ja not_active Expired - Fee Related
-
2006
- 2006-11-14 US US11/598,848 patent/US7918981B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
EP1379682A4 (de) | 2005-08-10 |
CN1376779A (zh) | 2002-10-30 |
US7153648B2 (en) | 2006-12-26 |
ATE466096T1 (de) | 2010-05-15 |
EP1379682B1 (de) | 2010-04-28 |
US20020182654A1 (en) | 2002-12-05 |
CA2440385C (en) | 2012-01-10 |
EP2116609B1 (de) | 2014-02-12 |
JP4411266B2 (ja) | 2010-02-10 |
CA2440385A1 (en) | 2002-10-03 |
JP2004522452A (ja) | 2004-07-29 |
CN100494360C (zh) | 2009-06-03 |
WO2002077269A1 (en) | 2002-10-03 |
US7918981B2 (en) | 2011-04-05 |
EP2116609A2 (de) | 2009-11-11 |
EP2116609A3 (de) | 2010-01-20 |
US20070128686A1 (en) | 2007-06-07 |
EP1379682A1 (de) | 2004-01-14 |
JP2006126195A (ja) | 2006-05-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE60236145D1 (de) | Zellisolierungsverfahren und verwendungen davon | |
CA2469878A1 (en) | Blood cell separating system | |
JP2012510272A5 (de) | ||
DE3376763D1 (en) | Centrifugation chambers for the cytodiagnostic preparation of epithelial cells and their application | |
HK1079960A1 (en) | Microfluidic device for cell separation and uses thereof | |
WO2007079149A3 (en) | Blood cell sorting methods and systems | |
DE60029528D1 (de) | Trennungsverfahren von Substanzen durch Dielektrophoresis | |
US20090186341A1 (en) | Receptacle for the Separation of Tumor Cells | |
ATE357660T1 (de) | Dielektrophoretisches verfahren und anordnung für hoch-durchsatz screening | |
WO2001096857A3 (en) | Method and apparatus for combined magnetophoretic and dielectrophoretic manipulation of analyte mixtures | |
CN104703699A (zh) | 分离或富集细胞的方法和成分 | |
WO1998054580A1 (en) | Device for isolating a component of a physiological sample | |
WO2009044552A1 (ja) | 分析用デバイスとこれを使用する分析装置および分析方法 | |
WO2004104164A3 (en) | Method and apparatus for preparing cell samples for intracellular antigen detection using flow cytometry | |
Chen et al. | A physical method for separating spermatozoa from epithelial cells in sexual assault evidence | |
DK1636344T3 (da) | Farmaceutisk kompatibel fremgangsmåde til oprensning af intakte bakterielle miniceller | |
US20220347686A1 (en) | Size-based asymmetric nanopore membrane (anm) filtration for high-efficiency exosome isolation, concentration, and fractionation | |
Nascimento et al. | Dielectrophoretic sorting on a microfabricated flow cytometer: Label free separation of Babesia bovis infected erythrocytes | |
US20120090998A1 (en) | Cell Separation | |
WO1995015681A1 (en) | Method and device for testing blood units for viral contamination | |
EP0587832B1 (de) | Verfahren zur testung von blutkonserven auf virale verunreinigung | |
Shaw et al. | A microfluidic “baby machine” for cell synchronization | |
WO2001077316A3 (en) | Method of nucleic acid recovery | |
Masri et al. | Challenges and advances in scale-up of label-free downstream processing for allogeneic cell therapies | |
WO2002016580A3 (en) | Flow-through system for lysate clarification and nucleic acid binding in a single step |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |