WO2002016580A3 - Flow-through system for lysate clarification and nucleic acid binding in a single step - Google Patents

Flow-through system for lysate clarification and nucleic acid binding in a single step Download PDF

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Publication number
WO2002016580A3
WO2002016580A3 PCT/US2001/024273 US0124273W WO0216580A3 WO 2002016580 A3 WO2002016580 A3 WO 2002016580A3 US 0124273 W US0124273 W US 0124273W WO 0216580 A3 WO0216580 A3 WO 0216580A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid binding
lysate
flow
receptacle
Prior art date
Application number
PCT/US2001/024273
Other languages
French (fr)
Other versions
WO2002016580A2 (en
Inventor
Brian Perry
Chau Lam
Original Assignee
Bio Rad Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories filed Critical Bio Rad Laboratories
Priority to AU2001281000A priority Critical patent/AU2001281000A1/en
Publication of WO2002016580A2 publication Critical patent/WO2002016580A2/en
Publication of WO2002016580A3 publication Critical patent/WO2002016580A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Abstract

Two flow-through receptacles, one containing a biologic lysate filtration medium and the other a nucleic acid binding medium, are constructed to be releasably joinable to form a continuous leak-free flow-through passage with the filtration medium at an upstream location in the passage and the binding medium at a downstream location. The joined receptacles are attached to a vacuum source arranged to draw a vacuum through the lower (nucleic acid binding) receptacle, or placed in a centrifuge, and a biologic lysate is placed in the upper (filtration) receptacle. With a single application of vacuum or centrifugation, the lysate is clarified and nucleic acids are extracted simultaneously. The receptacles are then separated and the purified and extracted nucleic acids are recovered from the nucleic acid binding receptacle.
PCT/US2001/024273 2000-08-24 2001-08-03 Flow-through system for lysate clarification and nucleic acid binding in a single step WO2002016580A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001281000A AU2001281000A1 (en) 2000-08-24 2001-08-03 Flow-through system for lysate clarification and nucleic acid binding in a single step

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US64547800A 2000-08-24 2000-08-24
US09/645,478 2000-08-24

Publications (2)

Publication Number Publication Date
WO2002016580A2 WO2002016580A2 (en) 2002-02-28
WO2002016580A3 true WO2002016580A3 (en) 2003-11-20

Family

ID=24589190

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/024273 WO2002016580A2 (en) 2000-08-24 2001-08-03 Flow-through system for lysate clarification and nucleic acid binding in a single step

Country Status (2)

Country Link
AU (1) AU2001281000A1 (en)
WO (1) WO2002016580A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691969B2 (en) 1994-12-12 2014-04-08 Life Technologies As Isolation of nucleic acid

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
US7240572B2 (en) 2004-02-18 2007-07-10 Millipore Corporation Vacuum assisted affinity chromatography device and method
WO2010075116A2 (en) * 2008-12-15 2010-07-01 Life Technologies Corporation Nucleic acid purification apparatus and method
GB201706680D0 (en) * 2017-04-27 2017-06-14 Ge Healthcare Uk Ltd Device and method for sample isolation

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4895706A (en) * 1986-10-28 1990-01-23 Costar Corporation Multi-well filter strip and composite assemblies
DE4127276A1 (en) * 1991-03-19 1992-09-24 Diagen Inst Molekularbio DEVICE AND METHOD FOR SEPARATING LIQUID SAMPLES
DE4139664A1 (en) * 1991-12-02 1993-06-03 Diagen Inst Molekularbio DEVICE AND METHOD FOR ISOLATING AND CLEANING NUCLEIC ACIDS
WO1995002049A1 (en) * 1993-07-09 1995-01-19 Cambridge Molecular Technologies Limited Purification method and apparatus
WO1996008500A1 (en) * 1994-09-14 1996-03-21 Qiagen Gmbh Process and device for isolating cellular substances such as nucleic acids from natural sources
WO1997008306A1 (en) * 1995-08-31 1997-03-06 Sequenom, Inc. Filtration processes, kits and devices for isolating plasmids
US6020186A (en) * 1990-10-26 2000-02-01 Qiagen Gmbh Device and process for isolating nucleic acids from cell suspensions

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4895706A (en) * 1986-10-28 1990-01-23 Costar Corporation Multi-well filter strip and composite assemblies
US6020186A (en) * 1990-10-26 2000-02-01 Qiagen Gmbh Device and process for isolating nucleic acids from cell suspensions
DE4127276A1 (en) * 1991-03-19 1992-09-24 Diagen Inst Molekularbio DEVICE AND METHOD FOR SEPARATING LIQUID SAMPLES
DE4139664A1 (en) * 1991-12-02 1993-06-03 Diagen Inst Molekularbio DEVICE AND METHOD FOR ISOLATING AND CLEANING NUCLEIC ACIDS
WO1995002049A1 (en) * 1993-07-09 1995-01-19 Cambridge Molecular Technologies Limited Purification method and apparatus
WO1996008500A1 (en) * 1994-09-14 1996-03-21 Qiagen Gmbh Process and device for isolating cellular substances such as nucleic acids from natural sources
WO1997008306A1 (en) * 1995-08-31 1997-03-06 Sequenom, Inc. Filtration processes, kits and devices for isolating plasmids

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691969B2 (en) 1994-12-12 2014-04-08 Life Technologies As Isolation of nucleic acid

Also Published As

Publication number Publication date
AU2001281000A1 (en) 2002-03-04
WO2002016580A2 (en) 2002-02-28

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