ATE357660T1 - Dielektrophoretisches verfahren und anordnung für hoch-durchsatz screening - Google Patents

Dielektrophoretisches verfahren und anordnung für hoch-durchsatz screening

Info

Publication number
ATE357660T1
ATE357660T1 AT02726415T AT02726415T ATE357660T1 AT E357660 T1 ATE357660 T1 AT E357660T1 AT 02726415 T AT02726415 T AT 02726415T AT 02726415 T AT02726415 T AT 02726415T AT E357660 T1 ATE357660 T1 AT E357660T1
Authority
AT
Austria
Prior art keywords
cages
entities
arrangement
liquid
throughput screening
Prior art date
Application number
AT02726415T
Other languages
English (en)
Inventor
Nicolo Manaresi
Gianni Medoro
Luigi Altomare
Marco Tartagni
Roberto Guerrieri
Original Assignee
Silicon Biosystems Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Silicon Biosystems Spa filed Critical Silicon Biosystems Spa
Application granted granted Critical
Publication of ATE357660T1 publication Critical patent/ATE357660T1/de

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Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G01N33/561Immunoelectrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT02726415T 2001-05-02 2002-05-02 Dielektrophoretisches verfahren und anordnung für hoch-durchsatz screening ATE357660T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT2001TO000411A ITTO20010411A1 (it) 2001-05-02 2001-05-02 Metodo e dispositivo per l'esecuzione di test e saggi ad alta processivita' ed alto valore biologico su cellule e/o composti.

Publications (1)

Publication Number Publication Date
ATE357660T1 true ATE357660T1 (de) 2007-04-15

Family

ID=11458822

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02726415T ATE357660T1 (de) 2001-05-02 2002-05-02 Dielektrophoretisches verfahren und anordnung für hoch-durchsatz screening

Country Status (8)

Country Link
US (1) US7699969B2 (de)
EP (1) EP1428027B1 (de)
AT (1) ATE357660T1 (de)
AU (1) AU2002256881A1 (de)
DE (1) DE60219040T2 (de)
ES (1) ES2284864T3 (de)
IT (1) ITTO20010411A1 (de)
WO (1) WO2002088702A2 (de)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2392977A (en) * 2002-09-13 2004-03-17 Suisse Electronique Microtech A fluidic dielectrophoretic system and method for analysing biomolecules
US7329545B2 (en) * 2002-09-24 2008-02-12 Duke University Methods for sampling a liquid flow
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
EP3144066A1 (de) 2004-05-28 2017-03-22 Board of Regents, The University of Texas System Programmierbare fluidverarbeiter
US20090155877A1 (en) * 2004-07-06 2009-06-18 Agency For Science Technology And Research Biochip for sorting and lysing biological samples
ITBO20040420A1 (it) 2004-07-07 2004-10-07 Type S R L Macchina per taglio e formatura di piattine metalliche
KR101198038B1 (ko) 2005-01-28 2012-11-06 듀크 유니버서티 인쇄 회로 기판 위의 액적 조작을 위한 기구 및 방법
ITBO20050481A1 (it) * 2005-07-19 2007-01-20 Silicon Biosystems S R L Metodo ed apparato per la manipolazione e/o l'individuazione di particelle
ITBO20050646A1 (it) 2005-10-26 2007-04-27 Silicon Biosystem S R L Metodo ed apparato per la caratterizzazione ed il conteggio di particelle
ITTO20060226A1 (it) 2006-03-27 2007-09-28 Silicon Biosystem S P A Metodo ed apparato per il processamento e o l'analisi e o la selezione di particelle, in particolare particelle biologiche
ITTO20060273A1 (it) * 2006-04-12 2007-10-13 Silicon Biosystem S P A Metodi ed apparati per la selezione e/o il processamento di particellle, in particolare per la lisi selettiva e/o ottimizzata di cellule
ITTO20060278A1 (it) * 2006-04-13 2007-10-14 Silicon Biosystem S P A Metodo per la selezione e/o il processamento di particelle, in particolare cellule
US8268246B2 (en) 2007-08-09 2012-09-18 Advanced Liquid Logic Inc PCB droplet actuator fabrication
ITTO20070771A1 (it) 2007-10-29 2009-04-30 Silicon Biosystems Spa Metodo e apparato per la identificazione e manipolazione di particelle
US10895575B2 (en) 2008-11-04 2021-01-19 Menarini Silicon Biosystems S.P.A. Method for identification, selection and analysis of tumour cells
IT1391619B1 (it) 2008-11-04 2012-01-11 Silicon Biosystems Spa Metodo per l'individuazione, selezione e analisi di cellule tumorali
EP2408562B1 (de) 2009-03-17 2018-03-07 Menarini Silicon Biosystems S.p.A. Mikrofluidvorrichtung zur isolation von zellen
US9005987B2 (en) * 2009-04-16 2015-04-14 University Of Southern California Methods for quantitative target detection and related devices and systems
IT1403518B1 (it) 2010-12-22 2013-10-31 Silicon Biosystems Spa Dispositivo microfluidico per la manipolazione di particelle
ITTO20110990A1 (it) 2011-10-28 2013-04-29 Silicon Biosystems Spa Metodo ed apparato per l'analisi ottica di particelle a basse temperature
ITBO20110766A1 (it) 2011-12-28 2013-06-29 Silicon Biosystems Spa Dispositivi, apparato, kit e metodo per il trattamento di un campione biologico
US9857333B2 (en) 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
DE102013106432A1 (de) * 2013-06-20 2014-12-24 Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG Optische Indikator-Einheit und Vorrichtung und Verfahren zur Bestimmung einer physikalisch-chemischen Eigenschaft eines Prozessmediums in einer prozesstechnischen Anlage
EP3060645B1 (de) 2013-10-22 2019-01-02 Berkeley Lights, Inc. Mikrofluidische vorrichtungen mit isolierenden stiften sowie verfahren zum testen biologischer mikroobjekte damit
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
SG11201602336UA (en) 2013-10-22 2016-05-30 Berkeley Lights Inc Micro-fluidic devices for assaying biological activity
US11192107B2 (en) 2014-04-25 2021-12-07 Berkeley Lights, Inc. DEP force control and electrowetting control in different sections of the same microfluidic apparatus
US20150306599A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. Providing DEP Manipulation Devices And Controllable Electrowetting Devices In The Same Microfluidic Apparatus
SG11201704558QA (en) 2014-12-08 2017-07-28 Berkeley Lights Inc Microfluidic device comprising lateral/vertical transistor structures and process of making and using same
EP3229961B1 (de) 2014-12-08 2019-11-13 Berkeley Lights, Inc. Betätigte mikrofluidische strukturen für gerichtete strömung in einer mikrofluidischen vorrichtung und verfahren zur verwendung davon
EP3230718B1 (de) 2014-12-09 2022-03-02 Berkeley Lights, Inc. Automatisierte detektion von assay-positiven bereichen oder von analytgehalten in mikrofluidischen vorrichtungen
CN113075113A (zh) 2014-12-09 2021-07-06 伯克利之光生命科技公司 微流体装置中微物体的自动检测和重新定位
WO2016094715A2 (en) 2014-12-10 2016-06-16 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
US10384204B2 (en) 2014-12-10 2019-08-20 Berkeley Lights, Inc. Systems for operating electrokinetic devices
KR102334118B1 (ko) 2015-04-22 2021-12-01 버클리 라잇츠, 인크. 마이크로유체 디바이스 상에서의 세포들의 동결 및 보관
US10751715B1 (en) 2015-04-22 2020-08-25 Berkeley Lights, Inc. Microfluidic reporter cell assay methods and kits thereof
WO2016172623A1 (en) 2015-04-22 2016-10-27 Berkeley Lights, Inc. Manipulation of cell nuclei in a micro-fluidic device
IL293366B2 (en) 2015-04-22 2023-10-01 Berkeley Lights Inc Kits and methods for preparing a microfluidic device for cell culture
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
IL299366A (en) 2015-11-23 2023-02-01 Berkeley Lights Inc Structures for microfluidic isolation are produced at the place of assembly, kits and their uses
IL259837B2 (en) 2015-12-08 2023-03-01 Berkeley Lights Inc Microfluidic devices and kits and their uses
AU2016381833C1 (en) 2015-12-30 2022-10-06 Berkeley Lights, Inc. Microfluidic devices for optically-driven convection and displacement, kits and methods thereof
WO2017117521A1 (en) 2015-12-31 2017-07-06 Berkeley Lights, Inc. Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide
CN109952313B (zh) 2016-01-15 2023-12-19 伯克利之光生命科技公司 生产患者特异性抗癌治疗剂的方法和其治疗方法
CN109922885B (zh) 2016-03-16 2022-05-10 伯克利之光生命科技公司 用于基因组编辑克隆的选择和传代的方法、系统和装置
US10675625B2 (en) 2016-04-15 2020-06-09 Berkeley Lights, Inc Light sequencing and patterns for dielectrophoretic transport
CA3020976A1 (en) 2016-04-15 2017-10-19 Berkeley Lights, Inc. Methods, systems and kits for in-pen assays
AU2017271673B2 (en) 2016-05-26 2022-04-14 Berkeley Lights, Inc. Covalently modified surfaces, kits, and methods of preparation and use
EP3487510B1 (de) 2016-07-21 2023-07-19 Berkeley Lights, Inc. Sortieren von t-lymphozyten in einer mikrofluidischen vorrichtung
EP3562594A4 (de) 2016-12-30 2020-09-09 The Regents of University of California Verfahren zur auswahl und erzeugung genomeditierten t-zellen
EP3721209B1 (de) 2017-10-15 2024-02-07 Berkeley Lights, Inc. Verfahren für in-pen-tests
NL2019937B1 (en) 2017-11-17 2019-05-24 Stichting Vu Method and system for optical force measurement
WO2020061499A1 (en) 2018-09-21 2020-03-26 Berkeley Lights, Inc. Functionalized well plate, methods of preparation and use thereof
WO2020223555A1 (en) 2019-04-30 2020-11-05 Berkeley Lights, Inc. Methods for encapsulating and assaying cells
CN116121031B (zh) * 2022-12-23 2024-03-12 重庆大学 用于单细胞筛选的多级化微流控芯片及其制备方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9301122D0 (en) * 1993-01-21 1993-03-10 Scient Generics Ltd Method of analysis/separation
US6071394A (en) 1996-09-06 2000-06-06 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US6387707B1 (en) * 1996-04-25 2002-05-14 Bioarray Solutions Array Cytometry
DE69737883T2 (de) * 1996-04-25 2008-03-06 Bioarray Solutions Ltd. Licht-regulierte, elektrokinetische zusammensetzung von partikeln an oberflächen
AU1742300A (en) * 1998-12-02 2000-06-19 Nanogen, Inc. Apparatus and methods for transport of charged biological materials
DE19859459A1 (de) * 1998-12-22 2000-06-29 Evotec Biosystems Ag Mikrosysteme zur Zellpermeation und Zellfusion
US6294063B1 (en) * 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
CN1181337C (zh) * 2000-08-08 2004-12-22 清华大学 微流体系统中实体分子的操纵方法及相关试剂盒
IT1309430B1 (it) * 1999-05-18 2002-01-23 Guerrieri Roberto Metodo ed apparato per la manipolazione di particelle per mezzo delladielettroforesi
US7198702B1 (en) * 1999-09-30 2007-04-03 Wako Pure Chemical Industries, Ltd. Method for separating substances using dielectrophoretic forces
CA2343873A1 (en) * 2000-04-12 2001-10-12 Wako Pure Chemical Industries Ltd. Electrode for dielectrophoretic apparatus, dielectrophoretic apparatus, methdo for manufacturing the same, and method for separating substances using the elctrode or dielectrophoretic apparatus
CN1325909C (zh) * 2000-09-27 2007-07-11 清华大学 用于微粒操纵与微粒导向的装置及其使用方法
AU2001297014A1 (en) * 2000-10-10 2002-04-22 Aviva Biosciences Corporation An integrated biochip system for sample preparation and analysis

Also Published As

Publication number Publication date
AU2002256881A1 (en) 2002-11-11
ITTO20010411A0 (it) 2001-05-02
US7699969B2 (en) 2010-04-20
EP1428027B1 (de) 2007-03-21
ITTO20010411A1 (it) 2002-11-02
WO2002088702A2 (en) 2002-11-07
DE60219040T2 (de) 2008-01-03
ES2284864T3 (es) 2007-11-16
WO2002088702A8 (en) 2004-05-21
WO2002088702A9 (en) 2004-06-24
DE60219040D1 (de) 2007-05-03
EP1428027A2 (de) 2004-06-16
WO2002088702A3 (en) 2004-03-25
US20040191789A1 (en) 2004-09-30

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