DE60228935D1 - Verfahren zur entwicklung einer zelle mit einem gewünschten phänotyp und entwickelte zellen - Google Patents

Verfahren zur entwicklung einer zelle mit einem gewünschten phänotyp und entwickelte zellen

Info

Publication number
DE60228935D1
DE60228935D1 DE60228935T DE60228935T DE60228935D1 DE 60228935 D1 DE60228935 D1 DE 60228935D1 DE 60228935 T DE60228935 T DE 60228935T DE 60228935 T DE60228935 T DE 60228935T DE 60228935 D1 DE60228935 D1 DE 60228935D1
Authority
DE
Germany
Prior art keywords
cell
cells
genes
denotes
composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60228935T
Other languages
German (de)
English (en)
Inventor
Neil Goldsmith
Alexandra M Soerensen
Soeren V Nielsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evolva Holding SA
Original Assignee
Evolva AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva AG filed Critical Evolva AG
Application granted granted Critical
Publication of DE60228935D1 publication Critical patent/DE60228935D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
DE60228935T 2001-01-25 2002-01-25 Verfahren zur entwicklung einer zelle mit einem gewünschten phänotyp und entwickelte zellen Expired - Lifetime DE60228935D1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200100129 2001-01-25
DKPA200100680 2001-05-01
US30043801P 2001-06-26 2001-06-26
PCT/DK2002/000057 WO2002059290A2 (en) 2001-01-25 2002-01-25 A method for evolving a cell having desired phenotype and envolved cells

Publications (1)

Publication Number Publication Date
DE60228935D1 true DE60228935D1 (de) 2008-10-30

Family

ID=34751435

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60228935T Expired - Lifetime DE60228935D1 (de) 2001-01-25 2002-01-25 Verfahren zur entwicklung einer zelle mit einem gewünschten phänotyp und entwickelte zellen

Country Status (9)

Country Link
US (1) US20050158860A1 (https=)
EP (1) EP1364005B1 (https=)
JP (2) JP2004527232A (https=)
AT (1) ATE408683T1 (https=)
AU (1) AU2002227884A1 (https=)
CA (1) CA2474146C (https=)
DE (1) DE60228935D1 (https=)
DK (1) DK1364005T3 (https=)
WO (1) WO2002059290A2 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60239386D1 (de) 2001-01-25 2011-04-21 Evolva Ltd Zellbibliothek
DE60228935D1 (de) * 2001-01-25 2008-10-30 Evolva Ltd Verfahren zur entwicklung einer zelle mit einem gewünschten phänotyp und entwickelte zellen
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
CA2511229A1 (en) * 2002-01-25 2003-07-31 Evolva Ltd. Methods for multiple parameter screening and evolution of cells to produce small molecules with multiple functionalities
AU2003247270A1 (en) * 2002-08-01 2004-03-03 Evolva Ltd Methods of mixing large numbers of heterologous genes
EP1866428A2 (en) 2005-03-18 2007-12-19 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
EP2078092A2 (en) 2006-09-28 2009-07-15 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
WO2011146833A1 (en) 2010-05-20 2011-11-24 Evolva Inc. Method of producing isoprenoid compounds in yeast
WO2013191950A2 (en) 2012-06-22 2013-12-27 Monsanto Technology Llc Unique modular vector design
CN104904715B (zh) * 2015-06-18 2017-04-12 青岛农业大学 丙戊酸钠在制备用于防治由植物病原菌引起的植物病害的杀菌剂中的用途
CN106701606B (zh) * 2016-12-19 2021-03-26 广东利世康低碳科技有限公司 一种能降解利用餐厨废弃物的基因工程产朊假丝酵母及其构建方法
CN112674081A (zh) * 2020-12-22 2021-04-20 宁波华木来生物科技有限公司 一种香榧及其假种皮的保鲜方法
CN113249442B (zh) * 2021-05-19 2023-03-14 中国科学院海洋研究所 一种筛查牡蛎不饱和脂肪酸含量相关甲基化修饰基因的方法
CN114176049B (zh) * 2022-01-20 2023-01-31 河南农业大学 一种同时提高室内繁育棉铃虫齿唇姬蜂出蜂率和雌虫数量的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5316922A (en) * 1992-04-15 1994-05-31 The United States Of America As Represented By Department Of Health And Human Services Method for indentifying and expressing proteins that recognize and adhere to specific probes
KR100501838B1 (ko) * 1995-04-24 2005-07-21 큐비스트 파마슈티컬즈 인코포레이티드 새로운 대사 경로를 만들고 이를 스크리닝하는 방법
EP1717322B1 (en) * 1997-01-17 2012-07-18 Codexis Mayflower Holdings, LLC Evolution of whole cells and organisms by recursive sequence recombination
DE60228935D1 (de) * 2001-01-25 2008-10-30 Evolva Ltd Verfahren zur entwicklung einer zelle mit einem gewünschten phänotyp und entwickelte zellen

Also Published As

Publication number Publication date
WO2002059290A3 (en) 2003-04-10
JP2010187692A (ja) 2010-09-02
ATE408683T1 (de) 2008-10-15
US20050158860A1 (en) 2005-07-21
WO2002059290A2 (en) 2002-08-01
EP1364005B1 (en) 2008-09-17
DK1364005T3 (da) 2009-02-02
JP2004527232A (ja) 2004-09-09
EP1364005A2 (en) 2003-11-26
WO2002059290A8 (en) 2004-04-15
CA2474146C (en) 2012-09-18
CA2474146A1 (en) 2002-08-01
AU2002227884A1 (en) 2002-08-06

Similar Documents

Publication Publication Date Title
DE60228935D1 (de) Verfahren zur entwicklung einer zelle mit einem gewünschten phänotyp und entwickelte zellen
Avramova et al. Matrix attachment regions and transcribed sequences within a long chromosomal continuum containing maize Adh1.
ATE458064T1 (de) Verfahren zur wirksamen rna-interferenz in säugerzellen
ATE513911T1 (de) Verfahren und zusammensetzungen für rna- interferenz
RU2013135291A (ru) Растение, обладающее повышенной устойчивостью или чувствительностью к ингибитору 4-hppd
ATE375394T1 (de) Concatemere unterschiedlich exprimierter multipler gene
DE69943389D1 (de) Verfahren und mittel zum erhalt von veränderten phänotypen
Engstler et al. Gene editing and scalable functional genomic screening in Leishmania species using the CRISPR/Cas9 cytosine base editor toolbox LeishBASEedit
IT202000028688A1 (it) Varianti della citidina deaminasi per l’editazione di basi
JP2005519633A5 (https=)
ATE527366T1 (de) Verfahren zur mischung von vielen heterologen genen
JP2004527232A5 (https=)
WO2005017127A3 (en) Rna interference compositions and methods
JP2005529624A (ja) ランダムｄｎａライブラリーと二本鎖ｒｎａライブラリー、その用途および生産方法
WO2024006926A9 (en) Editable cell lines
JP5665110B2 (ja) 外来ＤＮＡ断片由来の逆方向反復配列を含むＤＮＡ断片の調製方法及びＤＮＡ型ＲＮＡｉライブラリの調製方法
KR102035654B1 (ko) 항균 펩타이드를 생산하는 돌연변이 곤충 세포주 또는 이의 제조방법
EP4547846A2 (en) Editable cell lines
Hamshere Alternative pre-messenger RNA splicing of murine N-CAM and human tropomyosin in non-muscle and muscle cells
CN119836473A (zh) 一种提高CRISPR/Cas9介导的基因组敲入编辑效率的方法
CA2418352A1 (en) Assays for alternative lengthening of telomeres
ATE437952T1 (de) Verfahren zur herstellung umwelt sicherer transgener organismen
CN121320298A (zh) 基因编辑系统、基因编辑方法及所使用的逆转录酶
JP2023183417A5 (https=)
Reddy et al. Intrageneric fusions of isolated protoplasts from Ulva and Porphyra by electrofusion method

Legal Events

Date Code Title Description
8364 No opposition during term of opposition