DE60224457D1 - Methode zur modifizierung des plasmid-gehalts - Google Patents

Methode zur modifizierung des plasmid-gehalts

Info

Publication number
DE60224457D1
DE60224457D1 DE60224457T DE60224457T DE60224457D1 DE 60224457 D1 DE60224457 D1 DE 60224457D1 DE 60224457 T DE60224457 T DE 60224457T DE 60224457 T DE60224457 T DE 60224457T DE 60224457 D1 DE60224457 D1 DE 60224457D1
Authority
DE
Germany
Prior art keywords
plasmid content
modifying plasmid
modifying
chromosomal gene
plasmids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60224457T
Other languages
English (en)
Other versions
DE60224457T2 (de
Inventor
Jayaraman Gowrishankar
Rajendran Harinarayanan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CT FOR DNA FINGERPRINTING AND
Original Assignee
CT FOR DNA FINGERPRINTING AND
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CT FOR DNA FINGERPRINTING AND filed Critical CT FOR DNA FINGERPRINTING AND
Publication of DE60224457D1 publication Critical patent/DE60224457D1/de
Application granted granted Critical
Publication of DE60224457T2 publication Critical patent/DE60224457T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60224457T 2002-10-08 2002-10-28 Methode zur modifizierung des plasmid-gehalts Expired - Lifetime DE60224457T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US266510 2002-10-08
US10/266,510 US7176028B2 (en) 2002-10-08 2002-10-08 Method of altering levels of plasmids
PCT/IB2002/004500 WO2004033684A1 (en) 2002-10-08 2002-10-28 A method of altering levels of plasmids

Publications (2)

Publication Number Publication Date
DE60224457D1 true DE60224457D1 (de) 2008-02-14
DE60224457T2 DE60224457T2 (de) 2009-02-05

Family

ID=32042691

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60230688T Expired - Lifetime DE60230688D1 (de) 2002-10-08 2002-10-28 Methode zur Modifizierung des Plasmid-Gehalts in Bakterienzellen mit einer Mutation in dem chromosomalen Gen Rho
DE60224457T Expired - Lifetime DE60224457T2 (de) 2002-10-08 2002-10-28 Methode zur modifizierung des plasmid-gehalts

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE60230688T Expired - Lifetime DE60230688D1 (de) 2002-10-08 2002-10-28 Methode zur Modifizierung des Plasmid-Gehalts in Bakterienzellen mit einer Mutation in dem chromosomalen Gen Rho

Country Status (7)

Country Link
US (2) US7176028B2 (de)
EP (2) EP1674572B1 (de)
JP (1) JP4142649B2 (de)
AT (2) ATE419343T1 (de)
AU (1) AU2002339634A1 (de)
DE (2) DE60230688D1 (de)
WO (1) WO2004033684A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6569530B2 (ja) * 2013-10-02 2019-09-04 味の素株式会社 ヘパロサン生産細菌及びヘパロサンの製造法
WO2022075402A1 (ja) * 2020-10-09 2022-04-14 株式会社カネカ 大腸菌遺伝子改変株

Also Published As

Publication number Publication date
ATE419343T1 (de) 2009-01-15
WO2004033684A1 (en) 2004-04-22
US7176028B2 (en) 2007-02-13
EP1674572A1 (de) 2006-06-28
ATE382689T1 (de) 2008-01-15
EP1674572B1 (de) 2008-12-31
EP1551969B1 (de) 2008-01-02
US7220588B2 (en) 2007-05-22
JP4142649B2 (ja) 2008-09-03
EP1551969A1 (de) 2005-07-13
DE60230688D1 (de) 2009-02-12
JP2006501837A (ja) 2006-01-19
US20040067589A1 (en) 2004-04-08
AU2002339634A1 (en) 2004-05-04
DE60224457T2 (de) 2009-02-05
US20060246590A1 (en) 2006-11-02

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Legal Events

Date Code Title Description
8381 Inventor (new situation)

Inventor name: GOWRISHANKAR, JAYARAMAN, NACHARAM 500 076 HYDE, IN

Inventor name: HARINARAYANAN, RAJENDRAN, NACHARAM 500 076 HYD, IN

8332 No legal effect for de
8370 Indication related to discontinuation of the patent is to be deleted
8364 No opposition during term of opposition