AU2002223772A1 - Use of mutagenic dna polymerase for producing random mutations - Google Patents

Use of mutagenic dna polymerase for producing random mutations

Info

Publication number
AU2002223772A1
AU2002223772A1 AU2002223772A AU2377202A AU2002223772A1 AU 2002223772 A1 AU2002223772 A1 AU 2002223772A1 AU 2002223772 A AU2002223772 A AU 2002223772A AU 2377202 A AU2377202 A AU 2377202A AU 2002223772 A1 AU2002223772 A1 AU 2002223772A1
Authority
AU
Australia
Prior art keywords
dna polymerase
random mutations
producing random
mutagenic dna
random mutagenesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002223772A
Inventor
Khalil Bouayadi
Christophe Cazaux
Jean Sebastien Hoffmann
Hakim Kharrat
Thierry Louat
Laurence Servant
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MilleGen SA
Original Assignee
MilleGen SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MilleGen SA filed Critical MilleGen SA
Publication of AU2002223772A1 publication Critical patent/AU2002223772A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention concerns a method for random mutagenesis comprising the replication of a DNA sequence in the presence of an efficient amount of at least a mutase, for example a Pol β, the random mutagenesis rate being at least of the order of 1 mutation for 400 base pairs. The replication product, optionally recombined and amplified, is cloned in an expression vector so as to generate mutated polypeptides which will be selected on the basis of the desired property or properties.
AU2002223772A 2000-11-08 2001-11-07 Use of mutagenic dna polymerase for producing random mutations Abandoned AU2002223772A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0014333 2000-11-08
FR0014333A FR2816319B1 (en) 2000-11-08 2000-11-08 USE OF DNA MUTAGEN POLYMERASE FOR THE CREATION OF RANDOM MUTATIONS
PCT/FR2001/003450 WO2002038756A1 (en) 2000-11-08 2001-11-07 Use of mutagenic dna polymerase for producing random mutations

Publications (1)

Publication Number Publication Date
AU2002223772A1 true AU2002223772A1 (en) 2002-05-21

Family

ID=8856193

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002223772A Abandoned AU2002223772A1 (en) 2000-11-08 2001-11-07 Use of mutagenic dna polymerase for producing random mutations

Country Status (6)

Country Link
US (1) US7670809B2 (en)
EP (1) EP1332210B1 (en)
AT (1) ATE518953T1 (en)
AU (1) AU2002223772A1 (en)
FR (1) FR2816319B1 (en)
WO (1) WO2002038756A1 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1811031A1 (en) 2006-01-18 2007-07-25 Millegen Method for selecting a peptide or polypeptide which binds to a target molecule
WO2007137616A1 (en) * 2006-05-30 2007-12-06 Millegen Highly diversified antibody libraries
US20100081575A1 (en) * 2008-09-22 2010-04-01 Robert Anthony Williamson Methods for creating diversity in libraries and libraries, display vectors and methods, and displayed molecules
AU2009293640A1 (en) * 2008-09-22 2010-03-25 Calmune Corporation Methods and vectors for display of 2G12 -derived domain exchanged antibodies
CA2742969A1 (en) 2008-11-07 2010-05-14 Fabrus Llc Anti-dll4 antibodies and uses thereof
EP2233500A1 (en) 2009-03-20 2010-09-29 LFB Biotechnologies Optimized Fc variants
WO2011035205A2 (en) 2009-09-18 2011-03-24 Calmune Corporation Antibodies against candida, collections thereof and methods of use
US20120316071A1 (en) 2009-11-04 2012-12-13 Vaughn Smider Methods for affinity maturation-based antibody optimization
MX349622B (en) 2010-09-08 2017-08-07 Halozyme Inc Methods for assessing and identifying or evolving conditionally active therapeutic proteins.
WO2014096672A1 (en) 2012-12-17 2014-06-26 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Use of monoclonal antibodies for the treatment of inflammation and bacterial infections
FR3024453B1 (en) 2014-08-01 2018-06-29 Lab Francais Du Fractionnement PROCESS FOR PRODUCING VARIANTS HAVING FC HAVING ENHANCED SIALYLATION
FR3035879A1 (en) 2015-05-07 2016-11-11 Lab Francais Du Fractionnement MUTANTS FC WITH MODIFIED FUNCTIONAL ACTIVITY
FR3038517B1 (en) 2015-07-06 2020-02-28 Laboratoire Francais Du Fractionnement Et Des Biotechnologies USE OF MODIFIED FC FRAGMENTS IN IMMUNOTHERAPY
FR3058159B1 (en) 2016-10-28 2022-02-25 Lab Francais Du Fractionnement POLYPEPTIDE FC VARIANTS WITH AN INCREASED HALF-LIFE

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6255062B1 (en) * 1994-02-14 2001-07-03 California Institute Of Technology β-type DNA polymerases
US6165718A (en) * 1996-01-10 2000-12-26 Novo Nordisk A/S Novo Alle Method for in vivo production of a mutant library in cells
US6395524B2 (en) * 1996-11-27 2002-05-28 University Of Washington Thermostable polymerases having altered fidelity and method of identifying and using same
FR2757178B1 (en) * 1996-12-13 1999-03-05 Centre Nat Rech Scient USE OF A VECTOR EXPRESSING DNA POLYMERASE BETA AS A MEDICAMENT

Also Published As

Publication number Publication date
US20040110294A1 (en) 2004-06-10
FR2816319B1 (en) 2004-09-03
ATE518953T1 (en) 2011-08-15
WO2002038756A1 (en) 2002-05-16
EP1332210A1 (en) 2003-08-06
FR2816319A1 (en) 2002-05-10
EP1332210B1 (en) 2011-08-03
US7670809B2 (en) 2010-03-02

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