AU2002223772A1 - Use of mutagenic dna polymerase for producing random mutations - Google Patents
Use of mutagenic dna polymerase for producing random mutationsInfo
- Publication number
- AU2002223772A1 AU2002223772A1 AU2002223772A AU2377202A AU2002223772A1 AU 2002223772 A1 AU2002223772 A1 AU 2002223772A1 AU 2002223772 A AU2002223772 A AU 2002223772A AU 2377202 A AU2377202 A AU 2377202A AU 2002223772 A1 AU2002223772 A1 AU 2002223772A1
- Authority
- AU
- Australia
- Prior art keywords
- dna polymerase
- random mutations
- producing random
- mutagenic dna
- random mutagenesis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
Landscapes
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention concerns a method for random mutagenesis comprising the replication of a DNA sequence in the presence of an efficient amount of at least a mutase, for example a Pol β, the random mutagenesis rate being at least of the order of 1 mutation for 400 base pairs. The replication product, optionally recombined and amplified, is cloned in an expression vector so as to generate mutated polypeptides which will be selected on the basis of the desired property or properties.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR0014333 | 2000-11-08 | ||
FR0014333A FR2816319B1 (en) | 2000-11-08 | 2000-11-08 | USE OF DNA MUTAGEN POLYMERASE FOR THE CREATION OF RANDOM MUTATIONS |
PCT/FR2001/003450 WO2002038756A1 (en) | 2000-11-08 | 2001-11-07 | Use of mutagenic dna polymerase for producing random mutations |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2002223772A1 true AU2002223772A1 (en) | 2002-05-21 |
Family
ID=8856193
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2002223772A Abandoned AU2002223772A1 (en) | 2000-11-08 | 2001-11-07 | Use of mutagenic dna polymerase for producing random mutations |
Country Status (6)
Country | Link |
---|---|
US (1) | US7670809B2 (en) |
EP (1) | EP1332210B1 (en) |
AT (1) | ATE518953T1 (en) |
AU (1) | AU2002223772A1 (en) |
FR (1) | FR2816319B1 (en) |
WO (1) | WO2002038756A1 (en) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1811031A1 (en) | 2006-01-18 | 2007-07-25 | Millegen | Method for selecting a peptide or polypeptide which binds to a target molecule |
WO2007137616A1 (en) * | 2006-05-30 | 2007-12-06 | Millegen | Highly diversified antibody libraries |
US20100081575A1 (en) * | 2008-09-22 | 2010-04-01 | Robert Anthony Williamson | Methods for creating diversity in libraries and libraries, display vectors and methods, and displayed molecules |
AU2009293640A1 (en) * | 2008-09-22 | 2010-03-25 | Calmune Corporation | Methods and vectors for display of 2G12 -derived domain exchanged antibodies |
CA2742969A1 (en) | 2008-11-07 | 2010-05-14 | Fabrus Llc | Anti-dll4 antibodies and uses thereof |
EP2233500A1 (en) | 2009-03-20 | 2010-09-29 | LFB Biotechnologies | Optimized Fc variants |
WO2011035205A2 (en) | 2009-09-18 | 2011-03-24 | Calmune Corporation | Antibodies against candida, collections thereof and methods of use |
US20120316071A1 (en) | 2009-11-04 | 2012-12-13 | Vaughn Smider | Methods for affinity maturation-based antibody optimization |
MX349622B (en) | 2010-09-08 | 2017-08-07 | Halozyme Inc | Methods for assessing and identifying or evolving conditionally active therapeutic proteins. |
WO2014096672A1 (en) | 2012-12-17 | 2014-06-26 | Laboratoire Francais Du Fractionnement Et Des Biotechnologies | Use of monoclonal antibodies for the treatment of inflammation and bacterial infections |
FR3024453B1 (en) | 2014-08-01 | 2018-06-29 | Lab Francais Du Fractionnement | PROCESS FOR PRODUCING VARIANTS HAVING FC HAVING ENHANCED SIALYLATION |
FR3035879A1 (en) | 2015-05-07 | 2016-11-11 | Lab Francais Du Fractionnement | MUTANTS FC WITH MODIFIED FUNCTIONAL ACTIVITY |
FR3038517B1 (en) | 2015-07-06 | 2020-02-28 | Laboratoire Francais Du Fractionnement Et Des Biotechnologies | USE OF MODIFIED FC FRAGMENTS IN IMMUNOTHERAPY |
FR3058159B1 (en) | 2016-10-28 | 2022-02-25 | Lab Francais Du Fractionnement | POLYPEPTIDE FC VARIANTS WITH AN INCREASED HALF-LIFE |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6255062B1 (en) * | 1994-02-14 | 2001-07-03 | California Institute Of Technology | β-type DNA polymerases |
US6165718A (en) * | 1996-01-10 | 2000-12-26 | Novo Nordisk A/S Novo Alle | Method for in vivo production of a mutant library in cells |
US6395524B2 (en) * | 1996-11-27 | 2002-05-28 | University Of Washington | Thermostable polymerases having altered fidelity and method of identifying and using same |
FR2757178B1 (en) * | 1996-12-13 | 1999-03-05 | Centre Nat Rech Scient | USE OF A VECTOR EXPRESSING DNA POLYMERASE BETA AS A MEDICAMENT |
-
2000
- 2000-11-08 FR FR0014333A patent/FR2816319B1/en not_active Expired - Fee Related
-
2001
- 2001-11-07 EP EP01993683A patent/EP1332210B1/en not_active Expired - Lifetime
- 2001-11-07 US US10/416,424 patent/US7670809B2/en not_active Expired - Lifetime
- 2001-11-07 WO PCT/FR2001/003450 patent/WO2002038756A1/en not_active Application Discontinuation
- 2001-11-07 AU AU2002223772A patent/AU2002223772A1/en not_active Abandoned
- 2001-11-07 AT AT01993683T patent/ATE518953T1/en active
Also Published As
Publication number | Publication date |
---|---|
US20040110294A1 (en) | 2004-06-10 |
FR2816319B1 (en) | 2004-09-03 |
ATE518953T1 (en) | 2011-08-15 |
WO2002038756A1 (en) | 2002-05-16 |
EP1332210A1 (en) | 2003-08-06 |
FR2816319A1 (en) | 2002-05-10 |
EP1332210B1 (en) | 2011-08-03 |
US7670809B2 (en) | 2010-03-02 |
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