ATE419343T1 - Methode zur modifizierung des plasmid-gehalts in bakterienzellen mit einer mutation in dem chromosomalen gen rho - Google Patents

Methode zur modifizierung des plasmid-gehalts in bakterienzellen mit einer mutation in dem chromosomalen gen rho

Info

Publication number
ATE419343T1
ATE419343T1 AT06005978T AT06005978T ATE419343T1 AT E419343 T1 ATE419343 T1 AT E419343T1 AT 06005978 T AT06005978 T AT 06005978T AT 06005978 T AT06005978 T AT 06005978T AT E419343 T1 ATE419343 T1 AT E419343T1
Authority
AT
Austria
Prior art keywords
chromosomal gene
mutation
modifying
bacterial cells
plasmid content
Prior art date
Application number
AT06005978T
Other languages
English (en)
Inventor
Jayaraman Gowrishankar
Rajendran Harinarayanan
Original Assignee
Ct For Dna Fingerprinting And
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct For Dna Fingerprinting And filed Critical Ct For Dna Fingerprinting And
Application granted granted Critical
Publication of ATE419343T1 publication Critical patent/ATE419343T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT06005978T 2002-10-08 2002-10-28 Methode zur modifizierung des plasmid-gehalts in bakterienzellen mit einer mutation in dem chromosomalen gen rho ATE419343T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10/266,510 US7176028B2 (en) 2002-10-08 2002-10-08 Method of altering levels of plasmids

Publications (1)

Publication Number Publication Date
ATE419343T1 true ATE419343T1 (de) 2009-01-15

Family

ID=32042691

Family Applications (2)

Application Number Title Priority Date Filing Date
AT06005978T ATE419343T1 (de) 2002-10-08 2002-10-28 Methode zur modifizierung des plasmid-gehalts in bakterienzellen mit einer mutation in dem chromosomalen gen rho
AT02777683T ATE382689T1 (de) 2002-10-08 2002-10-28 Methode zur modifizierung des plasmid-gehalts

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT02777683T ATE382689T1 (de) 2002-10-08 2002-10-28 Methode zur modifizierung des plasmid-gehalts

Country Status (7)

Country Link
US (2) US7176028B2 (de)
EP (2) EP1674572B1 (de)
JP (1) JP4142649B2 (de)
AT (2) ATE419343T1 (de)
AU (1) AU2002339634A1 (de)
DE (2) DE60230688D1 (de)
WO (1) WO2004033684A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6569530B2 (ja) * 2013-10-02 2019-09-04 味の素株式会社 ヘパロサン生産細菌及びヘパロサンの製造法
WO2022075402A1 (ja) * 2020-10-09 2022-04-14 株式会社カネカ 大腸菌遺伝子改変株

Also Published As

Publication number Publication date
WO2004033684A1 (en) 2004-04-22
US7176028B2 (en) 2007-02-13
EP1674572A1 (de) 2006-06-28
ATE382689T1 (de) 2008-01-15
EP1674572B1 (de) 2008-12-31
EP1551969B1 (de) 2008-01-02
US7220588B2 (en) 2007-05-22
JP4142649B2 (ja) 2008-09-03
EP1551969A1 (de) 2005-07-13
DE60230688D1 (de) 2009-02-12
DE60224457D1 (de) 2008-02-14
JP2006501837A (ja) 2006-01-19
US20040067589A1 (en) 2004-04-08
AU2002339634A1 (en) 2004-05-04
DE60224457T2 (de) 2009-02-05
US20060246590A1 (en) 2006-11-02

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