DE60223243T2 - Acetat-freie aufreinigung von plasmid-dna unter verwendung von hydroxyapatit - Google Patents

Acetat-freie aufreinigung von plasmid-dna unter verwendung von hydroxyapatit Download PDF

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Publication number
DE60223243T2
DE60223243T2 DE60223243T DE60223243T DE60223243T2 DE 60223243 T2 DE60223243 T2 DE 60223243T2 DE 60223243 T DE60223243 T DE 60223243T DE 60223243 T DE60223243 T DE 60223243T DE 60223243 T2 DE60223243 T2 DE 60223243T2
Authority
DE
Germany
Prior art keywords
plasmid dna
hydroxyapatite
cell lysate
supernatant
acidification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60223243T
Other languages
German (de)
English (en)
Other versions
DE60223243D1 (de
Inventor
Cheryl S. Rodeo ABERIN
Samuel G. El Sobrante FRANKLIN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Application granted granted Critical
Publication of DE60223243D1 publication Critical patent/DE60223243D1/de
Publication of DE60223243T2 publication Critical patent/DE60223243T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Extraction Or Liquid Replacement (AREA)
DE60223243T 2001-05-23 2002-05-15 Acetat-freie aufreinigung von plasmid-dna unter verwendung von hydroxyapatit Expired - Lifetime DE60223243T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/866,093 US6406892B1 (en) 2001-05-23 2001-05-23 Acetate-free purification of plasmid DNA on hydroxyapatite
US866093 2001-05-23
PCT/US2002/015705 WO2002095047A1 (fr) 2001-05-23 2002-05-15 Purification sans acetate d'adn plasmidique sur hydroxyapatite

Publications (2)

Publication Number Publication Date
DE60223243D1 DE60223243D1 (de) 2007-12-13
DE60223243T2 true DE60223243T2 (de) 2008-08-07

Family

ID=25346898

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60223243T Expired - Lifetime DE60223243T2 (de) 2001-05-23 2002-05-15 Acetat-freie aufreinigung von plasmid-dna unter verwendung von hydroxyapatit

Country Status (7)

Country Link
US (1) US6406892B1 (fr)
EP (1) EP1390522B1 (fr)
JP (1) JP4125605B2 (fr)
AU (1) AU2002308753B2 (fr)
CA (1) CA2414788C (fr)
DE (1) DE60223243T2 (fr)
WO (1) WO2002095047A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100478138B1 (ko) * 2002-10-28 2005-03-21 주식회사 코젠바이오텍 Ｄｎａ 보관체 및 ｄｎａ 보관방법
JP4662578B2 (ja) * 2003-05-13 2011-03-30 ジェン−プロウブ インコーポレイテッド 抗生物質耐性微生物を同定するための方法およびキット
EP1559783A1 (fr) * 2004-01-29 2005-08-03 Qiagen GmbH Procédé pour la séparation chromatographique d'un mélange d'acides nucléiques
WO2006060282A2 (fr) 2004-11-30 2006-06-08 Merial Limited Melangeurs pour chimiolyse de cellules
US8907059B2 (en) * 2008-11-14 2014-12-09 Bio-Rad Laboratories, Inc. Phosphopeptide enrichment of compositions by fractionation on ceramic hydroxyapatite
CN116790578B (zh) * 2023-08-22 2023-12-12 赛奥斯博生物科技(北京)有限公司 一种基于碱裂解法快速纯化质粒的生产工艺

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE177786T1 (de) * 1990-05-28 1999-04-15 Univ Osaka Res Found Herstellungsverfahren für ein plasmid mit fähigkeiten zu expression und prozessierung nach translation eines retroviralen gens, so erhaltenes plasmid und dessen expressionsprodukt
DE69533552T2 (de) 1994-07-15 2005-09-22 Merck & Co., Inc. Ein verfahren für die plasmidreinigung in grossem massstab
US5834303A (en) 1994-11-04 1998-11-10 Tomy Seiko Co., Ltd. Method and device for extraction and purification of DNA
AR003122A1 (es) 1995-05-19 1998-07-08 Merck & Co Inc Un proceso para aislacion y purificacion de plasmidos en fermentadores de gran escala y el adn aislado y purificado obtenido mediante dicho proceso.
US5981735A (en) 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
US6011148A (en) 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
US5707812A (en) 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
US5910584A (en) * 1996-09-05 1999-06-08 Asahi Kogaku Kogyo Kabushiki Kaisha Method for isolating plasmid DNA
US5843731A (en) 1996-09-05 1998-12-01 Asahi Kogaku Kogyo Kabushiki Kaisha Method for purifying plasmid DNA on calcium phosphate compound
SE9703532D0 (sv) 1997-09-30 1997-09-30 Pharmacia Biotech Ab A process for the purification of plasmid DNA
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA

Also Published As

Publication number Publication date
EP1390522B1 (fr) 2007-10-31
JP4125605B2 (ja) 2008-07-30
JP2004520079A (ja) 2004-07-08
CA2414788A1 (fr) 2002-11-28
US6406892B1 (en) 2002-06-18
AU2002308753B2 (en) 2006-09-14
EP1390522A1 (fr) 2004-02-25
WO2002095047A1 (fr) 2002-11-28
DE60223243D1 (de) 2007-12-13
EP1390522A4 (fr) 2004-07-07
CA2414788C (fr) 2008-04-08

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