DE60220797T2 - Nucleinsäuresequenzen kodierend für enantioselektive amidasen - Google Patents
Nucleinsäuresequenzen kodierend für enantioselektive amidasen Download PDFInfo
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- DE60220797T2 DE60220797T2 DE60220797T DE60220797T DE60220797T2 DE 60220797 T2 DE60220797 T2 DE 60220797T2 DE 60220797 T DE60220797 T DE 60220797T DE 60220797 T DE60220797 T DE 60220797T DE 60220797 T2 DE60220797 T2 DE 60220797T2
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- Prior art keywords
- nucleic acid
- fermentation
- carboxylic acid
- acid sequence
- amide
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- 238000003556 assay Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- JPYQFYIEOUVJDU-UHFFFAOYSA-N beclamide Chemical compound ClCCC(=O)NCC1=CC=CC=C1 JPYQFYIEOUVJDU-UHFFFAOYSA-N 0.000 description 1
- 239000003782 beta lactam antibiotic agent Substances 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 239000011942 biocatalyst Substances 0.000 description 1
- 150000001642 boronic acid derivatives Chemical class 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 150000003857 carboxamides Chemical class 0.000 description 1
- 229960001265 ciclosporin Drugs 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 101150012893 dat gene Proteins 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229960002743 glutamine Drugs 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 239000012499 inoculation medium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- MAGPZHKLEZXLNU-UHFFFAOYSA-N mandelamide Chemical compound NC(=O)C(O)C1=CC=CC=C1 MAGPZHKLEZXLNU-UHFFFAOYSA-N 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 150000002736 metal compounds Chemical class 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 235000013923 monosodium glutamate Nutrition 0.000 description 1
- 239000004223 monosodium glutamate Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 229920002492 poly(sulfone) Polymers 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- NSFFHOGKXHRQEW-AIHSUZKVSA-N thiostrepton Chemical compound C([C@]12C=3SC=C(N=3)C(=O)N[C@H](C(=O)NC(/C=3SC[C@@H](N=3)C(=O)N[C@H](C=3SC=C(N=3)C(=O)N[C@H](C=3SC=C(N=3)[C@H]1N=1)[C@@H](C)OC(=O)C3=CC(=C4C=C[C@H]([C@@H](C4=N3)O)N[C@H](C(N[C@@H](C)C(=O)NC(=C)C(=O)N[C@@H](C)C(=O)N2)=O)[C@@H](C)CC)[C@H](C)O)[C@](C)(O)[C@@H](C)O)=C\C)[C@@H](C)O)CC=1C1=NC(C(=O)NC(=C)C(=O)NC(=C)C(N)=O)=CS1 NSFFHOGKXHRQEW-AIHSUZKVSA-N 0.000 description 1
- 229960004295 valine Drugs 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 150000003752 zinc compounds Chemical class 0.000 description 1
- 239000002132 β-lactam antibiotic Substances 0.000 description 1
- 229940124586 β-lactam antibiotics Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
- C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP01202822 | 2001-07-23 | ||
| EP01202821 | 2001-07-23 | ||
| EP01202822 | 2001-07-23 | ||
| EP01202821 | 2001-07-23 | ||
| PCT/NL2002/000471 WO2003010312A2 (en) | 2001-07-23 | 2002-07-15 | Nucleic acid sequences encoding enantioselective amidases |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60220797D1 DE60220797D1 (de) | 2007-08-02 |
| DE60220797T2 true DE60220797T2 (de) | 2008-04-10 |
Family
ID=26076964
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60220797T Expired - Lifetime DE60220797T2 (de) | 2001-07-23 | 2002-07-15 | Nucleinsäuresequenzen kodierend für enantioselektive amidasen |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US7241602B2 (enExample) |
| EP (1) | EP1409688B1 (enExample) |
| JP (1) | JP2004536608A (enExample) |
| CN (1) | CN1555416A (enExample) |
| AT (1) | ATE365216T1 (enExample) |
| AU (1) | AU2002318056A1 (enExample) |
| CA (1) | CA2454701A1 (enExample) |
| DE (1) | DE60220797T2 (enExample) |
| DK (1) | DK1409688T3 (enExample) |
| ES (1) | ES2286262T3 (enExample) |
| WO (1) | WO2003010312A2 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5190207B2 (ja) * | 2007-03-01 | 2013-04-24 | 三菱レイヨン株式会社 | 亜鉛又はその塩を含むアミダーゼの保存剤、活性賦活剤及び失活予防剤 |
| JP5138271B2 (ja) * | 2007-05-16 | 2013-02-06 | 三菱レイヨン株式会社 | 高活性アミダーゼ酵素液およびその調製方法 |
| EP2123767A1 (en) | 2008-05-20 | 2009-11-25 | DSM IP Assets B.V. | Preparation of epsilon-caprolactam via lysine cyclisation |
| EP2123768A1 (en) | 2008-05-20 | 2009-11-25 | DSM IP Assets B.V. | Preparation of (Z)-6,7-dihydro-1H-azepin-2(5H)-one |
| TW201002823A (en) * | 2008-05-20 | 2010-01-16 | Dsm Ip Assets Bv | Preparation of alpha-amino-epsilon-caprolactam via lysine cyclisation |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| LU74142A1 (enExample) * | 1976-01-08 | 1977-07-22 | ||
| NL9100038A (nl) * | 1991-01-11 | 1992-08-03 | Stamicarbon | Enzym-gekatalyseerde bereiding van optisch aktieve carbonzuren. |
| AU753138B2 (en) * | 1998-05-25 | 2002-10-10 | Qiagen Gmbh | Method for the isolation of ccc plasmid DNA |
| DE60042364D1 (de) * | 1999-04-16 | 2009-07-23 | Mitsubishi Rayon Co | Neue amidasegene |
| AU4436900A (en) | 1999-04-29 | 2000-11-17 | Dsm N.V. | Expression cassette for efficient production of a protein |
-
2002
- 2002-07-15 US US10/484,681 patent/US7241602B2/en not_active Expired - Fee Related
- 2002-07-15 CN CNA028181360A patent/CN1555416A/zh active Pending
- 2002-07-15 AU AU2002318056A patent/AU2002318056A1/en not_active Abandoned
- 2002-07-15 ES ES02747739T patent/ES2286262T3/es not_active Expired - Lifetime
- 2002-07-15 AT AT02747739T patent/ATE365216T1/de not_active IP Right Cessation
- 2002-07-15 CA CA002454701A patent/CA2454701A1/en not_active Abandoned
- 2002-07-15 EP EP02747739A patent/EP1409688B1/en not_active Expired - Lifetime
- 2002-07-15 WO PCT/NL2002/000471 patent/WO2003010312A2/en not_active Ceased
- 2002-07-15 DE DE60220797T patent/DE60220797T2/de not_active Expired - Lifetime
- 2002-07-15 JP JP2003515662A patent/JP2004536608A/ja active Pending
- 2002-07-15 DK DK02747739T patent/DK1409688T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| US20050079595A1 (en) | 2005-04-14 |
| CN1555416A (zh) | 2004-12-15 |
| AU2002318056A1 (en) | 2003-02-17 |
| ES2286262T3 (es) | 2007-12-01 |
| DE60220797D1 (de) | 2007-08-02 |
| EP1409688A2 (en) | 2004-04-21 |
| EP1409688B1 (en) | 2007-06-20 |
| WO2003010312A2 (en) | 2003-02-06 |
| ATE365216T1 (de) | 2007-07-15 |
| WO2003010312A3 (en) | 2003-10-30 |
| JP2004536608A (ja) | 2004-12-09 |
| CA2454701A1 (en) | 2003-02-06 |
| US7241602B2 (en) | 2007-07-10 |
| DK1409688T3 (da) | 2007-09-17 |
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| 8364 | No opposition during term of opposition |