DE60213703D1 - Verfahren zur Expression von Proteinen in einem in vitro Translationssystem mit Co-Expression von Helfer Proteinen für die Faltung - Google Patents

Verfahren zur Expression von Proteinen in einem in vitro Translationssystem mit Co-Expression von Helfer Proteinen für die Faltung

Info

Publication number
DE60213703D1
DE60213703D1 DE60213703T DE60213703T DE60213703D1 DE 60213703 D1 DE60213703 D1 DE 60213703D1 DE 60213703 T DE60213703 T DE 60213703T DE 60213703 T DE60213703 T DE 60213703T DE 60213703 D1 DE60213703 D1 DE 60213703D1
Authority
DE
Germany
Prior art keywords
proteins
expression
folding
vitro translation
translation system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60213703T
Other languages
English (en)
Other versions
DE60213703T2 (de
Inventor
Johannes Prof Dr Buchner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Original Assignee
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG, Roche Diagnostics GmbH filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of DE60213703D1 publication Critical patent/DE60213703D1/de
Publication of DE60213703T2 publication Critical patent/DE60213703T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
DE60213703T 2001-04-30 2002-04-27 Verfahren zur Expression von Proteinen in einem in vitro Translationssystem mit Co-Expression von Helfer Proteinen für die Faltung Expired - Fee Related DE60213703T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10121235 2001-04-30
DE10121235A DE10121235A1 (de) 2001-04-30 2001-04-30 Verfahren zur Expression von Proteinen in in vitro Translations-Systemen unter Ko-Expression von Faltungshelferproteinen

Publications (2)

Publication Number Publication Date
DE60213703D1 true DE60213703D1 (de) 2006-09-21
DE60213703T2 DE60213703T2 (de) 2007-08-16

Family

ID=7683311

Family Applications (2)

Application Number Title Priority Date Filing Date
DE10121235A Withdrawn DE10121235A1 (de) 2001-04-30 2001-04-30 Verfahren zur Expression von Proteinen in in vitro Translations-Systemen unter Ko-Expression von Faltungshelferproteinen
DE60213703T Expired - Fee Related DE60213703T2 (de) 2001-04-30 2002-04-27 Verfahren zur Expression von Proteinen in einem in vitro Translationssystem mit Co-Expression von Helfer Proteinen für die Faltung

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE10121235A Withdrawn DE10121235A1 (de) 2001-04-30 2001-04-30 Verfahren zur Expression von Proteinen in in vitro Translations-Systemen unter Ko-Expression von Faltungshelferproteinen

Country Status (6)

Country Link
US (1) US6929929B2 (de)
EP (1) EP1254962B1 (de)
JP (1) JP2002335959A (de)
AT (1) ATE335834T1 (de)
DE (2) DE10121235A1 (de)
ES (1) ES2269547T3 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10145694A1 (de) * 2001-09-17 2003-04-03 Roche Diagnostics Gmbh Verfahren zur Erhöhung der Löslichkeit, der Expressionsrate und der Aktivität von Proteinen während der rekombinanten Herstellung
AU2002349296A1 (en) * 2001-11-30 2003-06-10 Novozymes A/S Method for generating a site-specific library of variants
CA2463719A1 (en) 2003-04-05 2004-10-05 F. Hoffmann-La Roche Ag Nucleotide analogs with six membered rings
GB0329681D0 (en) * 2003-12-23 2004-01-28 Delta Biotechnology Ltd Gene expression technique
JP4561243B2 (ja) * 2004-08-27 2010-10-13 株式会社豊田中央研究所 無細胞タンパク質合成系のための媒体
JP5631533B2 (ja) * 2004-12-23 2014-11-26 ノボザイムス バイオファーマ デーコー アクティーゼルスカブ 遺伝子発現技術
GB0512707D0 (en) * 2005-06-22 2005-07-27 Delta Biotechnology Ltd Gene expression technique

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993025681A1 (en) 1992-06-11 1993-12-23 New York University A cytoplasmic chaperonin and methods of making and using it
CA2159899A1 (en) 1993-04-08 1994-10-27 Wieslaw Kudlicki Cell free system for protein synthesis and use of chaperone proteins therein
JPH09173078A (ja) 1995-09-14 1997-07-08 Tadayuki Imanaka 分子シャペロンを用いる蛋白質の製造方法
JP3344618B2 (ja) 1997-06-20 2002-11-11 株式会社エイチ・エス・ピー研究所 シャペロン発現プラスミド
AU5338199A (en) * 1998-08-09 2000-02-28 Geron Corporation Methods and compositions for inhibiting or stimulating telomerase assembly
JP4249832B2 (ja) 1998-12-28 2009-04-08 タカラバイオ株式会社 トリガーファクター発現プラスミド
US6495360B1 (en) * 1999-05-28 2002-12-17 Photogen, Inc. Method for enhanced protein stabilization and for production of cell lines useful for production of such stabilized proteins

Also Published As

Publication number Publication date
DE10121235A1 (de) 2002-10-31
ATE335834T1 (de) 2006-09-15
ES2269547T3 (es) 2007-04-01
EP1254962A1 (de) 2002-11-06
EP1254962B1 (de) 2006-08-09
DE60213703T2 (de) 2007-08-16
JP2002335959A (ja) 2002-11-26
US20020192743A1 (en) 2002-12-19
US6929929B2 (en) 2005-08-16

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee