DE60212642T2 - Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen - Google Patents

Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen Download PDF

Info

Publication number
DE60212642T2
DE60212642T2 DE60212642T DE60212642T DE60212642T2 DE 60212642 T2 DE60212642 T2 DE 60212642T2 DE 60212642 T DE60212642 T DE 60212642T DE 60212642 T DE60212642 T DE 60212642T DE 60212642 T2 DE60212642 T2 DE 60212642T2
Authority
DE
Germany
Prior art keywords
agt
fusion protein
process according
label
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60212642T
Other languages
German (de)
English (en)
Other versions
DE60212642D1 (de
Inventor
Kai Johnsson
Susanne Gendreizig
Antje Keppler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ECOLE POLYTECH
Ecole Polytechnique Federale de Lausanne EPFL
Original Assignee
ECOLE POLYTECH
Ecole Polytechnique Federale de Lausanne EPFL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ECOLE POLYTECH, Ecole Polytechnique Federale de Lausanne EPFL filed Critical ECOLE POLYTECH
Application granted granted Critical
Publication of DE60212642D1 publication Critical patent/DE60212642D1/de
Publication of DE60212642T2 publication Critical patent/DE60212642T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE60212642T 2001-04-10 2002-04-05 Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen Expired - Lifetime DE60212642T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28276601P 2001-04-10 2001-04-10
US282766P 2001-04-10
PCT/GB2002/001636 WO2002083937A2 (en) 2001-04-10 2002-04-05 Methods using o6-alkylguanine-dna alkyltransferases

Publications (2)

Publication Number Publication Date
DE60212642D1 DE60212642D1 (de) 2006-08-03
DE60212642T2 true DE60212642T2 (de) 2007-06-14

Family

ID=23083030

Family Applications (3)

Application Number Title Priority Date Filing Date
DE60212642T Expired - Lifetime DE60212642T2 (de) 2001-04-10 2002-04-05 Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen
DE60230689T Expired - Lifetime DE60230689D1 (de) 2001-04-10 2002-04-05 Verfahren zur Verwendung von O6-Alkylguanin-DNS Alkyltransferasen
DE60237792T Expired - Lifetime DE60237792D1 (de) 2001-04-10 2002-04-05 Verfahren zur Verwendung von O6-Alkylguanin-DNS Alkyltransferasen

Family Applications After (2)

Application Number Title Priority Date Filing Date
DE60230689T Expired - Lifetime DE60230689D1 (de) 2001-04-10 2002-04-05 Verfahren zur Verwendung von O6-Alkylguanin-DNS Alkyltransferasen
DE60237792T Expired - Lifetime DE60237792D1 (de) 2001-04-10 2002-04-05 Verfahren zur Verwendung von O6-Alkylguanin-DNS Alkyltransferasen

Country Status (14)

Country Link
US (2) US7939284B2 (https=)
EP (4) EP1410023B1 (https=)
JP (2) JP4195815B2 (https=)
KR (1) KR20040039194A (https=)
CN (2) CN1295510C (https=)
AT (3) ATE419378T1 (https=)
AU (1) AU2002251257B2 (https=)
CA (1) CA2443570A1 (https=)
DE (3) DE60212642T2 (https=)
DK (1) DK1410023T3 (https=)
IL (1) IL157880A0 (https=)
NZ (3) NZ528594A (https=)
WO (1) WO2002083937A2 (https=)
ZA (1) ZA200307442B (https=)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006501286A (ja) * 2002-10-03 2006-01-12 エコル・ポリテクニック・フェデラル・ドゥ・ローザンヌ(エーペーエフエル) O6−アルキルグアニン−dnaアルキルトランスフェラーゼ
KR20050062588A (ko) * 2002-10-03 2005-06-23 에콜 폴리테크니크 페데랄레 드 로잔 O6-알킬구아닌-dna 알킬트랜스퍼라제용 기질
EP1594962B1 (en) 2003-01-31 2014-08-27 Promega Corporation Covalent tethering of functional groups to proteins
US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
AU2004242249A1 (en) * 2003-05-23 2004-12-02 Epfl-Ecole Polytechnique Federale De Lausanne Methods for protein labeling based on acyl carrier protein
US8163479B2 (en) 2004-03-02 2012-04-24 Ecole Polytechnique Federale De Lausanne Specific substrates for O6-alkylguanine-DNA alkyltransferase
US7888090B2 (en) 2004-03-02 2011-02-15 Ecole Polytechnique Federale De Lausanne Mutants of O6-alkylguanine-DNA alkyltransferase
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US7825096B2 (en) 2004-09-08 2010-11-02 The United States Of America As Represented By The Department Of Health And Human Services O6-alkylguanine-DNA alkyltransferase inactivators and beta-glucuronidase cleavable prodrugs
US7273714B2 (en) * 2005-03-11 2007-09-25 Vion Pharmaceuticals, Inc. Alkylguanyltransferase assays
WO2006114409A1 (en) * 2005-04-27 2006-11-02 Covalys Biosciences Ag Pyrimidines reacting with o6-alkylguanine-dna alkyltransferase
FR2890174B1 (fr) 2005-08-30 2009-04-24 Cis Bio Internat Sa Procede pour la mise en evidence d'un processus biologique par mesure d'un fret
FR2890446B1 (fr) 2005-09-05 2008-04-18 Cis Bio Internat Sa Methode de detection d'interaction intracellulaire entre bio-molecules
CA2656192C (en) 2006-06-30 2016-05-03 Ambit Biosciences Corporation Detectable nucleic acid tag
EP2374875B1 (en) 2006-10-30 2015-09-02 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and funcitional expression
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
MX2009005936A (es) * 2006-12-05 2010-01-15 Lasergen Inc Nucleotidos y nucleosidos marcados de foto-escision y nucleotidos y nucleosidos marcados y metodos para su uso en secunciacion de adn.
EP1978093A1 (en) * 2007-04-04 2008-10-08 Ecole Polytechnique Fédérale de Lausanne (EPFL) Method of crosslinking two objects of interest
GB0711560D0 (en) * 2007-06-14 2007-07-25 Innova Biosciences Ltd Improvements in or relating to production of conjugates
WO2009058814A1 (en) 2007-10-29 2009-05-07 New England Biolabs, Inc. Methods and compositions for detection and enrichment of target small rnas
EP2093564A1 (en) 2008-02-25 2009-08-26 Technische Universität Dresden Medizinische Fakultät Carl Gustav Carus Method for distinguishing secretory granules of different ages
KR101677772B1 (ko) 2008-06-11 2016-11-18 레이저젠, 인코퍼레이티드 뉴클레오타이드 및 뉴클레오사이드 그리고 시퀀싱에서의 이들의 이용 방법
FR2936245B1 (fr) * 2008-09-23 2012-07-06 Cis Bio Int Nouveaux substrats d'o6-alkylguanine-adn alkyltransferase et ses mutants.
DE112011100297B4 (de) 2010-01-20 2017-08-24 New England Biolabs, Inc. Zusammensetzungen, Verfahren und damit in Zusammenhang stehende Anwendungen zum Spalten modifizierter DNA
WO2012037361A2 (en) 2010-09-15 2012-03-22 Endacea, Inc. Methods of use and kit for measurement of lipopolysaccharide with a time resolved fluorescence based assay
MX342195B (es) 2011-09-13 2016-09-20 Lasergen Inc Nucleótidos de terminación de rápida fotodescomposición 5-metoxi, 3' -oh no bloqueados y métodos para secuenciación de ácido nucleico.
MX349562B (es) * 2011-12-09 2017-08-02 Pasteur Institut Ensayo de inmuno deteccion multiplex.
US9200260B2 (en) 2012-03-15 2015-12-01 New England Biolabs, Inc. Compositions and methods for the transfer of a hexosamine to a modified nucleotide in a nucleic acid
JP6441893B2 (ja) 2013-03-19 2018-12-19 ディレクティド・ジェノミクス・エル・エル・シー 標的配列の濃縮
JP5781589B2 (ja) * 2013-12-20 2015-09-24 レーザーゲン インコーポレイテッド 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにdna配列決定におけるそれらの使用法
WO2016003795A1 (en) 2014-07-02 2016-01-07 New England Biolabs, Inc. Universal n-glycan binding reagent
JP2017537657A (ja) 2014-12-11 2017-12-21 ニユー・イングランド・バイオレイブス・インコーポレイテツド 標的配列の濃縮
CN104844710B (zh) * 2015-04-13 2018-02-02 西北大学 定向固定化pega复合型树酯的制备方法及应用
CN109661407A (zh) 2016-06-06 2019-04-19 西北大学 融合蛋白构建体
CN113277985B (zh) 2016-07-20 2023-07-28 纳莹(上海)生物科技有限公司 一种荧光探针及其制备方法和用途
US10934533B1 (en) 2018-10-10 2021-03-02 New England Biolabs, Inc. Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA
CN111253417B (zh) * 2019-09-04 2022-03-25 大连民族大学 一种嘌呤衍生物的合成及应用
JP7727628B2 (ja) 2019-12-03 2025-08-21 アラマー バイオサイエンシーズ, インク. 核酸結合免疫サンドイッチアッセイ(nulisa)
EP4103701A1 (en) 2020-02-13 2022-12-21 New England Biolabs, Inc. Variant family d dna polymerases
WO2021163559A1 (en) 2020-02-13 2021-08-19 New England Biolabs, Inc. Variant family d dna polymerases
WO2021183921A1 (en) 2020-03-12 2021-09-16 New England Biolabs, Inc. A rapid diagnostic test for lamp
CN112010983A (zh) * 2020-08-11 2020-12-01 西北大学 止咳平喘中药活性成分靶向筛选方法及试剂盒
WO2022040443A2 (en) 2020-08-21 2022-02-24 New England Biolabs, Inc. A rapid diagnostic test for lamp
US11993792B2 (en) 2021-05-27 2024-05-28 New England Biolabs, Inc. DNase I variants, compositions, methods, and kits
WO2022250676A1 (en) 2021-05-27 2022-12-01 New England Biolabs, Inc. Dnase i variants, compositions, methods, and kits
CA3236352A1 (en) 2021-11-24 2023-06-01 Romualdas Vaisvila Double-stranded dna deaminases
WO2023197015A1 (en) 2022-04-08 2023-10-12 New England Biolabs, Inc. Compositions and analysis of dephosphorylated oligoribonucleotides
EP4658673A1 (en) 2023-02-01 2025-12-10 New England Biolabs, Inc. Duplex-specific dnases
US20250115953A1 (en) 2023-10-05 2025-04-10 New England Biolabs, Inc. Methylcytosine-Selective Deaminases and Uses Thereof
WO2025129193A1 (en) 2023-12-15 2025-06-19 New England Biolabs, Inc. Glycosyltransferases and related methods
WO2025137453A1 (en) 2023-12-20 2025-06-26 New England Biolabs, Inc. Compositions, methods, kits, and instruments for analyzing rna structure
GB202400129D0 (en) 2024-01-04 2024-02-21 Cambridge Entpr Ltd Nucleic acid barcodes
WO2025224010A1 (en) 2024-04-24 2025-10-30 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V. Improved o6-alkylguanine-dna alkyltransferase substrates
WO2025227034A1 (en) 2024-04-26 2025-10-30 New England Biolabs, Inc. Salt active nuclease compositions and methods

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9224888D0 (en) * 1992-11-27 1993-01-13 Univ Singapore Monoclonal antibody
DK0702683T3 (da) * 1993-06-08 2004-02-02 Cancer Rec Tech Ltd 06-substituerede guaninderivater, fremgangsmåde til fremstilling deraf og anvendelse deraf i behandling af tumorceller
DE19903895A1 (de) 1999-02-01 2000-08-03 Kai Johnsson Selektive Immobilisierung und Nachweis der O·6·-Alkylguanin-DNA-Alkyltransferase in der aktiven Form

Also Published As

Publication number Publication date
US8367361B2 (en) 2013-02-05
WO2002083937A2 (en) 2002-10-24
NZ540410A (en) 2006-06-30
EP2211177B1 (en) 2014-07-16
EP2037271A1 (en) 2009-03-18
US20110201514A1 (en) 2011-08-18
CN1527941A (zh) 2004-09-08
CN1975422A (zh) 2007-06-06
CN1295510C (zh) 2007-01-17
EP1696234A1 (en) 2006-08-30
CA2443570A1 (en) 2002-10-24
CN1975422B (zh) 2012-09-05
EP1410023B1 (en) 2006-06-21
JP4195815B2 (ja) 2008-12-17
IL157880A0 (en) 2004-03-28
ATE482395T1 (de) 2010-10-15
JP2008154587A (ja) 2008-07-10
JP2004532028A (ja) 2004-10-21
KR20040039194A (ko) 2004-05-10
ATE331220T1 (de) 2006-07-15
ATE419378T1 (de) 2009-01-15
NZ537939A (en) 2005-08-26
EP2211177A1 (en) 2010-07-28
HK1068406A1 (en) 2005-04-29
DK1410023T3 (da) 2006-10-09
US7939284B2 (en) 2011-05-10
DE60237792D1 (de) 2010-11-04
AU2002251257B2 (en) 2007-08-30
EP1696234B1 (en) 2008-12-31
US20040115130A1 (en) 2004-06-17
DE60230689D1 (de) 2009-02-12
ZA200307442B (en) 2004-06-30
WO2002083937A3 (en) 2004-02-26
EP1410023A2 (en) 2004-04-21
DE60212642D1 (de) 2006-08-03
NZ528594A (en) 2005-07-29
JP4226053B2 (ja) 2009-02-18
EP2037271B1 (en) 2010-09-22

Similar Documents

Publication Publication Date Title
DE60212642T2 (de) Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen
JP2004532028A5 (https=)
AU2002251257A1 (en) Methods of Using O6-alkylguanine-DNA Alkyltransferases
DE60015319T2 (de) Test zur gleichzeitigen Messung von verschiedenen enzymischen Aktivitäten
Antimonova et al. Changing times: fluorescence-lifetime analysis of amyloidogenic SF-IAPP fusion protein
EP3622292B1 (en) Genetically encoded potassium ion indicators
EP1334208A2 (de) Verfahren zur markierung chemischer substanzen
EP1405079B1 (de) Modulierung der wechselwirkung zwischen evh1-domänen
EP1181377A2 (de) Proteinkonjugate auf basis von lumazinsynthase, verfahren zu deren herstellung und deren verwendung
HK40017877B (en) Genetically encoded potassium ion indicators
HK40017877A (en) Genetically encoded potassium ion indicators
Török et al. Studying the Spatial Distribution of Ca2+-Binding Proteins: How Does it Work for Calmodulin?
Müntjes Towards visualizing translation by single-molecule imaging of nascent peptides within hyphae of Ustilago maydis
HK1068406B (en) Methods using o6 - alkylguanine - dna alkyltransferases

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: MAIWALD PATENTANWALTSGESELLSCHAFT MBH, 80335 MUENC