DE60212642T2 - Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen - Google Patents

Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen Download PDF

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Publication number
DE60212642T2
DE60212642T2 DE60212642T DE60212642T DE60212642T2 DE 60212642 T2 DE60212642 T2 DE 60212642T2 DE 60212642 T DE60212642 T DE 60212642T DE 60212642 T DE60212642 T DE 60212642T DE 60212642 T2 DE60212642 T2 DE 60212642T2
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DE
Germany
Prior art keywords
agt
fusion protein
process according
label
protein
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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DE60212642T
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German (de)
English (en)
Other versions
DE60212642D1 (de
Inventor
Kai Johnsson
Susanne Gendreizig
Antje Keppler
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ECOLE POLYTECH
Ecole Polytechnique Federale de Lausanne EPFL
Original Assignee
ECOLE POLYTECH
Ecole Polytechnique Federale de Lausanne EPFL
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Publication of DE60212642D1 publication Critical patent/DE60212642D1/de
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Publication of DE60212642T2 publication Critical patent/DE60212642T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE60212642T 2001-04-10 2002-04-05 Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen Expired - Lifetime DE60212642T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28276601P 2001-04-10 2001-04-10
US282766P 2001-04-10
PCT/GB2002/001636 WO2002083937A2 (en) 2001-04-10 2002-04-05 Methods using o6-alkylguanine-dna alkyltransferases

Publications (2)

Publication Number Publication Date
DE60212642D1 DE60212642D1 (de) 2006-08-03
DE60212642T2 true DE60212642T2 (de) 2007-06-14

Family

ID=23083030

Family Applications (3)

Application Number Title Priority Date Filing Date
DE60212642T Expired - Lifetime DE60212642T2 (de) 2001-04-10 2002-04-05 Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen
DE60230689T Expired - Lifetime DE60230689D1 (de) 2001-04-10 2002-04-05 Verfahren zur Verwendung von O6-Alkylguanin-DNS Alkyltransferasen
DE60237792T Expired - Lifetime DE60237792D1 (de) 2001-04-10 2002-04-05 Verfahren zur Verwendung von O6-Alkylguanin-DNS Alkyltransferasen

Family Applications After (2)

Application Number Title Priority Date Filing Date
DE60230689T Expired - Lifetime DE60230689D1 (de) 2001-04-10 2002-04-05 Verfahren zur Verwendung von O6-Alkylguanin-DNS Alkyltransferasen
DE60237792T Expired - Lifetime DE60237792D1 (de) 2001-04-10 2002-04-05 Verfahren zur Verwendung von O6-Alkylguanin-DNS Alkyltransferasen

Country Status (14)

Country Link
US (2) US7939284B2 (https=)
EP (4) EP1696234B1 (https=)
JP (2) JP4195815B2 (https=)
KR (1) KR20040039194A (https=)
CN (2) CN1975422B (https=)
AT (3) ATE419378T1 (https=)
AU (1) AU2002251257B2 (https=)
CA (1) CA2443570A1 (https=)
DE (3) DE60212642T2 (https=)
DK (1) DK1410023T3 (https=)
IL (1) IL157880A0 (https=)
NZ (3) NZ537939A (https=)
WO (1) WO2002083937A2 (https=)
ZA (1) ZA200307442B (https=)

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US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
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EP1730298B1 (en) * 2004-03-02 2015-11-11 EPFL Ecole Polytechnique Fédérale de Lausanne Specific substrates for o6-alkylguanine-dna alkyltransferase
EP1720981A2 (en) 2004-03-02 2006-11-15 EPFL Ecole Polytechnique Fédérale de Lausanne Mutants of o6-alkylguanine-dna alkyltransferase
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US7825096B2 (en) 2004-09-08 2010-11-02 The United States Of America As Represented By The Department Of Health And Human Services O6-alkylguanine-DNA alkyltransferase inactivators and beta-glucuronidase cleavable prodrugs
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EP2087107A2 (en) 2006-10-30 2009-08-12 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
KR101504640B1 (ko) * 2006-12-05 2015-03-20 레이저젠, 인코퍼레이티드 광절단가능 표지된 뉴클레오티드와 뉴클레오시드, 표지된 뉴클레오티드와 뉴클레오시드, 그리고 dna 염기서열분석에서 이들의 이용 방법
EP1978093A1 (en) * 2007-04-04 2008-10-08 Ecole Polytechnique Fédérale de Lausanne (EPFL) Method of crosslinking two objects of interest
GB0711560D0 (en) * 2007-06-14 2007-07-25 Innova Biosciences Ltd Improvements in or relating to production of conjugates
ATE522810T1 (de) * 2007-10-29 2011-09-15 New England Biolabs Inc Verfahren und zusammensetzungen zum nachweis und zur anreicherung kleiner ziel-rnas
EP2093564A1 (en) 2008-02-25 2009-08-26 Technische Universität Dresden Medizinische Fakultät Carl Gustav Carus Method for distinguishing secretory granules of different ages
NZ590265A (en) 2008-06-11 2012-11-30 Lasergen Inc Nucleotides and nucleosides and methods for their use in dna sequencing
FR2936245B1 (fr) * 2008-09-23 2012-07-06 Cis Bio Int Nouveaux substrats d'o6-alkylguanine-adn alkyltransferase et ses mutants.
DE112011100297B4 (de) 2010-01-20 2017-08-24 New England Biolabs, Inc. Zusammensetzungen, Verfahren und damit in Zusammenhang stehende Anwendungen zum Spalten modifizierter DNA
JP6043288B2 (ja) 2010-09-15 2016-12-14 エンダセア, インコーポレイテッド 時間分解蛍光ベースのアッセイによるリポ多糖の測定のための使用方法およびキット
WO2013040257A1 (en) 2011-09-13 2013-03-21 Lasergen, Inc. 5-methoxy. 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
BR112014013745B1 (pt) * 2011-12-09 2022-06-14 Institut Pasteur Método de teste in vitro, kit de detecção e seu uso, método in vitro de diagnóstico, método de fabricação de kit, e teste de imunosseleção multiplex
US9267117B2 (en) 2012-03-15 2016-02-23 New England Biolabs, Inc. Mapping cytosine modifications
US9708658B2 (en) 2013-03-19 2017-07-18 New England Biolabs, Inc. Enrichment of target sequences
JP5781589B2 (ja) * 2013-12-20 2015-09-24 レーザーゲン インコーポレイテッド 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにdna配列決定におけるそれらの使用法
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CN104844710B (zh) * 2015-04-13 2018-02-02 西北大学 定向固定化pega复合型树酯的制备方法及应用
US12098215B2 (en) 2016-06-06 2024-09-24 Northwestern University Fusion protein construct
CN113444046B (zh) 2016-07-20 2023-10-27 纳莹(上海)生物科技有限公司 一种荧光探针及其制备方法和用途
US10934533B1 (en) 2018-10-10 2021-03-02 New England Biolabs, Inc. Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA
CN111253417B (zh) * 2019-09-04 2022-03-25 大连民族大学 一种嘌呤衍生物的合成及应用
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CN112010983A (zh) * 2020-08-11 2020-12-01 西北大学 止咳平喘中药活性成分靶向筛选方法及试剂盒
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WO2022250676A1 (en) 2021-05-27 2022-12-01 New England Biolabs, Inc. Dnase i variants, compositions, methods, and kits
US11993792B2 (en) 2021-05-27 2024-05-28 New England Biolabs, Inc. DNase I variants, compositions, methods, and kits
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DE19903895A1 (de) * 1999-02-01 2000-08-03 Kai Johnsson Selektive Immobilisierung und Nachweis der O·6·-Alkylguanin-DNA-Alkyltransferase in der aktiven Form

Also Published As

Publication number Publication date
EP2037271A1 (en) 2009-03-18
US20110201514A1 (en) 2011-08-18
US20040115130A1 (en) 2004-06-17
ZA200307442B (en) 2004-06-30
DE60212642D1 (de) 2006-08-03
JP2004532028A (ja) 2004-10-21
NZ537939A (en) 2005-08-26
EP1696234B1 (en) 2008-12-31
CN1975422A (zh) 2007-06-06
ATE482395T1 (de) 2010-10-15
CN1295510C (zh) 2007-01-17
EP2211177A1 (en) 2010-07-28
WO2002083937A2 (en) 2002-10-24
WO2002083937A3 (en) 2004-02-26
JP4226053B2 (ja) 2009-02-18
US8367361B2 (en) 2013-02-05
IL157880A0 (en) 2004-03-28
CN1975422B (zh) 2012-09-05
CA2443570A1 (en) 2002-10-24
HK1068406A1 (en) 2005-04-29
EP1410023A2 (en) 2004-04-21
US7939284B2 (en) 2011-05-10
JP2008154587A (ja) 2008-07-10
DK1410023T3 (da) 2006-10-09
NZ528594A (en) 2005-07-29
AU2002251257B2 (en) 2007-08-30
JP4195815B2 (ja) 2008-12-17
CN1527941A (zh) 2004-09-08
EP1410023B1 (en) 2006-06-21
DE60237792D1 (de) 2010-11-04
DE60230689D1 (de) 2009-02-12
ATE331220T1 (de) 2006-07-15
EP2211177B1 (en) 2014-07-16
KR20040039194A (ko) 2004-05-10
ATE419378T1 (de) 2009-01-15
EP2037271B1 (en) 2010-09-22
EP1696234A1 (en) 2006-08-30
NZ540410A (en) 2006-06-30

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Representative=s name: MAIWALD PATENTANWALTSGESELLSCHAFT MBH, 80335 MUENC