DE602007006908D1 - Verfahren zur Verstärkung von Nukleinsäure aus Zellen von Mikroorganismen mittels eines nonplanaren festen Substrats - Google Patents

Verfahren zur Verstärkung von Nukleinsäure aus Zellen von Mikroorganismen mittels eines nonplanaren festen Substrats

Info

Publication number
DE602007006908D1
DE602007006908D1 DE602007006908T DE602007006908T DE602007006908D1 DE 602007006908 D1 DE602007006908 D1 DE 602007006908D1 DE 602007006908 T DE602007006908 T DE 602007006908T DE 602007006908 T DE602007006908 T DE 602007006908T DE 602007006908 D1 DE602007006908 D1 DE 602007006908D1
Authority
DE
Germany
Prior art keywords
microorganisms
cells
nucleic acid
solid substrate
amplifying nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602007006908T
Other languages
English (en)
Inventor
Sung-Young Jeong
Kyu-Youn Hwang
Joon-Ho Kim
Jung-Im Han
Hun-Joo Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Samsung Electronics Co Ltd
Original Assignee
Samsung Electronics Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020060079053A external-priority patent/KR100917465B1/ko
Priority claimed from KR1020060079056A external-priority patent/KR100837401B1/ko
Priority claimed from KR1020060079054A external-priority patent/KR100813264B1/ko
Priority claimed from KR1020060079055A external-priority patent/KR100813265B1/ko
Application filed by Samsung Electronics Co Ltd filed Critical Samsung Electronics Co Ltd
Publication of DE602007006908D1 publication Critical patent/DE602007006908D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
DE602007006908T 2006-08-21 2007-08-21 Verfahren zur Verstärkung von Nukleinsäure aus Zellen von Mikroorganismen mittels eines nonplanaren festen Substrats Active DE602007006908D1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR1020060079053A KR100917465B1 (ko) 2006-08-21 2006-08-21 비평면 형상의 고체 지지체를 이용하여 미생물을 분리하는방법 및 미생물분리 장치
KR1020060079056A KR100837401B1 (ko) 2006-08-21 2006-08-21 비평면 형상의 고체 지지체를 이용하여 미생물로부터핵산을 분리하는 방법,상기 분리된 핵산을 주형으로 하여핵산을 증폭하는 방법 및 상기 고체 지지체를 포함하는핵산 분리 및 증폭 장치
KR1020060079054A KR100813264B1 (ko) 2006-08-21 2006-08-21 비평면 형상의 고체 지지체를 이용하여 미생물로부터핵산을 증폭하는 방법
KR1020060079055A KR100813265B1 (ko) 2006-08-21 2006-08-21 비평면 형상의 고체 지지체를 이용하여 미생물로부터핵산을 증폭하는 방법

Publications (1)

Publication Number Publication Date
DE602007006908D1 true DE602007006908D1 (de) 2010-07-15

Family

ID=38980858

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602007006908T Active DE602007006908D1 (de) 2006-08-21 2007-08-21 Verfahren zur Verstärkung von Nukleinsäure aus Zellen von Mikroorganismen mittels eines nonplanaren festen Substrats

Country Status (4)

Country Link
US (3) US20080044884A1 (de)
EP (3) EP1972688B1 (de)
JP (1) JP2008048735A (de)
DE (1) DE602007006908D1 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1712284B1 (de) * 2005-04-15 2012-10-10 Samsung Electronics Co., Ltd. Methode zur Zelltrennung mit Benutzung hydrophober fester Träger
US20080044884A1 (en) * 2006-08-21 2008-02-21 Samsung Electronics Co., Ltd. Method and device for separating cells from a sample using a nonplanar solid substrate
US8158411B2 (en) 2006-08-21 2012-04-17 Samsung Electronics Co., Ltd. Method of separating microorganism using nonplanar solid substrate and device for separating microorganism using the same
KR101569832B1 (ko) * 2008-11-19 2015-11-18 삼성전자주식회사 시료 중의 게놈 ｄｎａ 및 플라스미드 ｄｎａ를 서로 분리하는 방법 및 그를 위한 키트
EP2411134B1 (de) 2009-03-25 2015-02-18 Eastman Kodak Company Tropfenerzeugung
JP5622189B2 (ja) * 2010-01-28 2014-11-12 国立大学法人東京工業大学 単一細胞分離用プレート
GB201010237D0 (en) 2010-06-18 2010-07-21 Lgc Ltd Methods and apparatuses
US8790926B2 (en) * 2011-02-16 2014-07-29 Empire Technology Development Llc Tissue engineering supports and methods therefor
US9670540B2 (en) 2011-07-21 2017-06-06 Cornell University Methods and devices for DNA sequencing and molecular diagnostics
WO2015103331A1 (en) * 2013-12-31 2015-07-09 Canon U.S. Life Sciences, Inc. Methods and systems for continuous flow cell lysis in a microfluidic device
KR102207922B1 (ko) 2014-03-06 2021-01-26 삼성전자주식회사 반코마이신 저항성 엔테로코커스 특이적인 프라이머 세트, 그를 포함하는 조성물 및 시료 중 반코마이신 저항성 엔테로코커스 속 미생물을 검출하는 방법
KR102287811B1 (ko) 2014-10-31 2021-08-09 삼성전자주식회사 2개 표면을 결합시키는 방법 및 그에 의하여 제조된 구조체, 및 상기 구조체를 포함하는 미세유동 장치
CN109219395B (zh) * 2017-05-08 2020-07-10 Tcm生物科学股份有限公司 用于收集含有子宫颈细胞的阴道分泌物样本的装置
GB201805479D0 (en) * 2018-04-03 2018-05-16 Momentum Bioscience Ltd Microorganism separation and detection
BR112022000722A2 (pt) * 2019-07-16 2022-03-08 Meliolabs Inc Métodos e dispositivos para curva de melting digital baseada em célula única
US11481230B2 (en) * 2020-12-17 2022-10-25 Oracle International Corporation Techniques for modifying a compute instance

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5928484A (ja) 1982-08-11 1984-02-15 Mitsui Toatsu Chem Inc Ｌ−アミノ酸の単離方法
EP0208647A3 (de) 1985-07-09 1989-03-08 Protein Foods Group Inc. Immobilisierungsträger für chemische und physikalische Verfahren und Methoden zu ihrer Herstellung
JPH0623758B2 (ja) * 1987-04-09 1994-03-30 テルモ株式会社 Ｂリンパ球分離材、分離方法および分離器
JP2852307B2 (ja) 1989-02-09 1999-02-03 吉富製薬株式会社 ウイルスの除去された蛋白質製剤の製造法
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5085779A (en) 1989-06-07 1992-02-04 J. T. Baker, Inc. Polyethyleneimine matrixes for affinity chromatography
GB9003253D0 (en) * 1990-02-13 1990-04-11 Amersham Int Plc Precipitating polymers
IES65976B2 (en) 1994-03-01 1995-11-29 Teagasc Agric Food Dev Authori Rapid detection of bacteria in liquid cultures
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5707799A (en) * 1994-09-30 1998-01-13 Abbott Laboratories Devices and methods utilizing arrays of structures for analyte capture
WO1998046797A1 (en) 1997-04-16 1998-10-22 Immunological Associates Of Denver Nucleic acid archiving
GB9709728D0 (en) 1997-05-13 1997-07-02 Dynal As Single step method
WO1999009042A2 (en) * 1997-08-13 1999-02-25 Cepheid Microstructures for the manipulation of fluid samples
ATE386044T1 (de) 1997-12-06 2008-03-15 Invitrogen Corp Isolierung von nukleinsäuren
ES2309022T3 (es) 1997-12-24 2008-12-16 Cepheid Dispositivo y procedimiento para lisis.
AU2692900A (en) * 1999-03-17 2000-10-04 Japan Immuno Research Laboratories Co., Ltd. Treatment of disease
JP2001098007A (ja) 1999-09-29 2001-04-10 Kawamura Inst Of Chem Res 親水性成形物およびその製造法
GB0013658D0 (en) * 2000-06-05 2000-07-26 Dynal Asa Nucleic acid isolation
US20040241828A1 (en) 2001-07-24 2004-12-02 Marie-Christine Huau Automated process for detecting pathogenic organisms in water
WO2004029221A2 (en) 2002-09-27 2004-04-08 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
JP5441290B2 (ja) * 2002-10-04 2014-03-12 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 核酸精製システムの媒体上に核酸を保存するツールとして、化学物質を用いる方法および材料
US20060000772A1 (en) * 2002-11-29 2006-01-05 Toru Sano Separation apparatus and separation method
SE0400073D0 (sv) 2003-04-04 2004-01-14 Appear Sweden Hb Antibacterial material
KR100953612B1 (ko) 2003-06-02 2010-04-20 삼성에스디아이 주식회사 생체물질 고정용 기판 및 이의 제조방법
WO2005093065A1 (en) 2004-03-16 2005-10-06 Roche Diagnostics Gmbh Improved method of isolating nucleic acids
JP4202391B2 (ja) * 2004-03-17 2008-12-24 パナソニック株式会社 バイオチップの製造方法、プローブ溶液、および、バイオチップ
JP2005295969A (ja) 2004-04-16 2005-10-27 Hitachi Medical Corp 細胞分離装置
KR100594408B1 (ko) 2004-12-17 2006-06-30 한국과학기술연구원 초음파장 및 진행파 유전영동을 이용한 세포 분리 장치
KR100647335B1 (ko) 2005-04-15 2006-11-23 삼성전자주식회사 소수성 고체 지지체를 이용한 세포 분리 방법
EP1712284B1 (de) 2005-04-15 2012-10-10 Samsung Electronics Co., Ltd. Methode zur Zelltrennung mit Benutzung hydrophober fester Träger
KR100682945B1 (ko) 2005-05-24 2007-02-15 삼성전자주식회사 상 분리를 이용한 세포 분리 방법 및 키트
US8158411B2 (en) 2006-08-21 2012-04-17 Samsung Electronics Co., Ltd. Method of separating microorganism using nonplanar solid substrate and device for separating microorganism using the same
US20080044884A1 (en) 2006-08-21 2008-02-21 Samsung Electronics Co., Ltd. Method and device for separating cells from a sample using a nonplanar solid substrate
KR100862660B1 (ko) * 2006-09-25 2008-10-10 삼성전자주식회사 단일 비드에 의한 핵산의 분리 및 정제 방법 및 장치

Also Published As

Publication number Publication date
EP1972688A1 (de) 2008-09-24
EP1892295A3 (de) 2008-03-26
EP1900807A3 (de) 2008-04-02
EP1972688B1 (de) 2010-06-02
US20080044884A1 (en) 2008-02-21
EP1892295B1 (de) 2012-07-25
US7919278B2 (en) 2011-04-05
US20080070282A1 (en) 2008-03-20
US20080044864A1 (en) 2008-02-21
EP1892295A2 (de) 2008-02-27
EP1900807B1 (de) 2014-02-26
EP1900807A2 (de) 2008-03-19
JP2008048735A (ja) 2008-03-06

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