DE602005023133D1 - Prozess und reagenzien für extraktion von rns von fraktionierten blutleukozyten - Google Patents

Prozess und reagenzien für extraktion von rns von fraktionierten blutleukozyten

Info

Publication number
DE602005023133D1
DE602005023133D1 DE602005023133T DE602005023133T DE602005023133D1 DE 602005023133 D1 DE602005023133 D1 DE 602005023133D1 DE 602005023133 T DE602005023133 T DE 602005023133T DE 602005023133 T DE602005023133 T DE 602005023133T DE 602005023133 D1 DE602005023133 D1 DE 602005023133D1
Authority
DE
Germany
Prior art keywords
rna
reagents
extraction
blood leukocytes
wbcs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE602005023133T
Other languages
German (de)
English (en)
Inventor
Marianna Goldrick
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ambion Inc
Original Assignee
Ambion Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ambion Inc filed Critical Ambion Inc
Publication of DE602005023133D1 publication Critical patent/DE602005023133D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
DE602005023133T 2004-03-16 2005-03-16 Prozess und reagenzien für extraktion von rns von fraktionierten blutleukozyten Expired - Lifetime DE602005023133D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/801,982 US20050208501A1 (en) 2004-03-16 2004-03-16 Process and reagents for extraction of RNA from fractionated blood leukocytes
PCT/US2005/008826 WO2005090984A1 (en) 2004-03-16 2005-03-16 Process and reagents for extraction of rna from fractionated blood leukocytes

Publications (1)

Publication Number Publication Date
DE602005023133D1 true DE602005023133D1 (de) 2010-10-07

Family

ID=34963160

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005023133T Expired - Lifetime DE602005023133D1 (de) 2004-03-16 2005-03-16 Prozess und reagenzien für extraktion von rns von fraktionierten blutleukozyten

Country Status (6)

Country Link
US (1) US20050208501A1 (enExample)
EP (1) EP1728078B1 (enExample)
JP (2) JP2007529225A (enExample)
AT (1) ATE479095T1 (enExample)
DE (1) DE602005023133D1 (enExample)
WO (1) WO2005090984A1 (enExample)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
US8979770B2 (en) * 2006-02-24 2015-03-17 Merck Sharp & Dohme Corp. Extraction and diagnostic fluid devices, systems and methods of use
DE102006009469A1 (de) * 2006-02-27 2007-09-06 Alexakis, Antonis, Dr. med. dent. Antigenbildung gegen Viren, Krebszellen, sowie gegen Zellen die mit Viren infiziert sind durch RNA sowie DNA Extraktion
US20070207483A1 (en) * 2006-03-02 2007-09-06 Bio-Rad Laboratories, Inc. BUFFERS FOR DETECTION OF mRNA SEPARATED IN A MICROFLUIDIC DEVICE
EP2147120B1 (en) 2007-05-03 2014-04-02 Roche Diagnostics GmbH Improved lysis and reverse transcription for mrna quantification
WO2009009457A1 (en) * 2007-07-06 2009-01-15 University Of Louisville Research Foundation, Inc. Alzheimer's disease-specific micro-rna microarray and related methods
ES2539856T3 (es) * 2008-08-01 2015-07-06 F. Hoffmann-La Roche Ag Lisis mejorada y transcripción reversa para la cuantificación de mRNA
US20110027862A1 (en) * 2009-06-29 2011-02-03 Life Technologies Corporation Sample stabilization
US8774488B2 (en) 2010-03-11 2014-07-08 Cellscape Corporation Method and device for identification of nucleated red blood cells from a maternal blood sample
WO2011162326A1 (ja) * 2010-06-25 2011-12-29 株式会社日本遺伝子研究所 Rna鎖の保存溶液
KR101420094B1 (ko) * 2010-10-27 2014-07-17 (주)바이오니아 다양한 생체시료분석을 위한 전자동실시간정량증폭장비, 다양한 생체시료분석을 위한 자동정제 및 반응준비 장치, 전자동 핵산정제 및 실시간 정량 유전자증폭 방법, 전자동 핵산정제방법, 실시간정량pcr을 이용한 병원균의 전자동 생균수검사방법, 정량면역pcr을 이용한 전자동 항원농도획득방법 및 타겟항원에 라벨링된 부착용 타겟핵산의 정제방법
DE102011080853B4 (de) 2011-08-11 2014-03-27 Axagarius Gmbh & Co. Kg Verfahren zur Isolierung von RNA aus Volblutproben
WO2015009970A1 (en) 2013-07-18 2015-01-22 Erythron Llc Spectroscopic measurements with parallel array detector
US20150338338A1 (en) 2014-02-28 2015-11-26 Erythron, Llc Method and Apparatus for Determining Markers of Health by Analysis of Blood
CA2962956C (en) 2014-10-20 2023-09-05 Gen-Probe Incorporated Red blood cell lysis solution
EP3282937A4 (en) 2015-04-14 2018-11-21 Nueon Inc. Method and apparatus for determining markers of health by analysis of blood
JP2018531783A (ja) * 2015-10-14 2018-11-01 ノボザイムス アクティーゼルスカブ 水濾過膜の洗浄
US10000742B2 (en) 2015-11-19 2018-06-19 General Electric Company Device and method of collection for RNA viruses
WO2017165403A1 (en) 2016-03-21 2017-09-28 Nueon Inc. Porous mesh spectrometry methods and apparatus
DE202017007129U1 (de) 2016-04-27 2019-08-29 Gen-Probe Incorporated Lysereagenz für Blutzellen
DK3472351T3 (da) 2016-06-15 2020-11-09 Univ Muenchen Ludwig Maximilians Enkeltmolekylepåvisning eller -kvantificering ved hjælp af DNA-nanoteknologi
WO2018085699A1 (en) 2016-11-04 2018-05-11 Nueon Inc. Combination blood lancet and analyzer
CN109402238A (zh) * 2018-12-19 2019-03-01 广州海思医疗科技有限公司 用于血液、口腔拭子进行pcr的裂解液及试剂盒

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4652525A (en) * 1978-04-19 1987-03-24 The Regents Of The University Of California Recombinant bacterial plasmids containing the coding sequences of insulin genes
CA1301606C (en) * 1986-05-02 1992-05-26 David H. Gillespie Chaotropic method for evaluating nucleic acids in a biological sample
US4923620A (en) 1987-10-20 1990-05-08 Pall Corporation Device for depletion of the leukocyte content of blood and blood components
US4925572A (en) * 1987-10-20 1990-05-15 Pall Corporation Device and method for depletion of the leukocyte content of blood and blood components
US4880548A (en) 1988-02-17 1989-11-14 Pall Corporation Device and method for separating leucocytes from platelet concentrate
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5229012A (en) 1989-05-09 1993-07-20 Pall Corporation Method for depletion of the leucocyte content of blood and blood components
US5344561A (en) 1989-05-09 1994-09-06 Pall Corporation Device for depletion of the leucocyte content of blood and blood components
US5045449A (en) * 1989-06-14 1991-09-03 Thomas Jefferson University Methods of detecting a genetic predisposition for vascular aneurysms
JPH0825886B2 (ja) * 1989-07-14 1996-03-13 テルモ株式会社 白血球捕捉用フィルター材およびその製造方法
US5100564A (en) 1990-11-06 1992-03-31 Pall Corporation Blood collection and processing system
US5457024A (en) * 1993-01-22 1995-10-10 Aprogenex, Inc. Isolation of fetal erythrocytes
JPH08280384A (ja) * 1995-04-18 1996-10-29 Toyobo Co Ltd 有核細胞を含む試料から核酸または蛋白質を回収する方法
EP0830364A1 (en) * 1995-06-08 1998-03-25 Progen Industries Limited Method and apparatus for dna extraction
US5702884A (en) * 1996-03-12 1997-12-30 Johnson & Johnson Clinical Diagnostics, Inc. Whole blood sample preparation for polymerase chain reaction using ammonium chloride and a carboxylic acid or metal carboxylate for selective red blood cell lysis
US6544751B1 (en) 1997-04-08 2003-04-08 Pall Corporation Methods of harvesting rare cells from blood products
US6204375B1 (en) 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
GB9912631D0 (en) * 1999-05-28 1999-07-28 Nat Blood Authority The Cell counter
WO2000077253A1 (en) * 1999-06-16 2000-12-21 Hitachi, Ltd. Apparatus and method for gene examination
EP1220944B1 (en) 1999-09-24 2010-09-15 Ambion, Inc. Nuclease inhibitor cocktail
AU2001263836A1 (en) * 2000-04-11 2001-10-23 Bureco Ag Method of nucleic acid recovery
AU2001286499A1 (en) * 2000-08-16 2002-02-25 Matthew Baker Transfusion medicine leukodepletion filter devices as a source of genetic material for genotyping studies
US7108969B1 (en) * 2000-09-08 2006-09-19 Affymetrix, Inc. Methods for detecting and diagnosing oral cancer
DE60231701D1 (de) * 2001-01-25 2009-05-07 Quiagen Gmbh Ammoniumsulfat zur aufhebung von inhibitorischen effekten
EP2163652B2 (en) 2002-01-28 2023-05-31 Life Technologies Corporation Preparing crude biological extracts using protease, suitable for preparing cDNA
CN1650027B (zh) * 2002-04-24 2010-04-21 日立化成研究中心公司 用于全血mRNA的高通量定量的装置和方法

Also Published As

Publication number Publication date
JP2011200236A (ja) 2011-10-13
EP1728078A1 (en) 2006-12-06
ATE479095T1 (de) 2010-09-15
US20050208501A1 (en) 2005-09-22
JP2007529225A (ja) 2007-10-25
EP1728078B1 (en) 2010-08-25
WO2005090984A1 (en) 2005-09-29

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