DE602005023133D1 - Prozess und reagenzien für extraktion von rns von fraktionierten blutleukozyten - Google Patents

Prozess und reagenzien für extraktion von rns von fraktionierten blutleukozyten

Info

Publication number
DE602005023133D1
DE602005023133D1 DE602005023133T DE602005023133T DE602005023133D1 DE 602005023133 D1 DE602005023133 D1 DE 602005023133D1 DE 602005023133 T DE602005023133 T DE 602005023133T DE 602005023133 T DE602005023133 T DE 602005023133T DE 602005023133 D1 DE602005023133 D1 DE 602005023133D1
Authority
DE
Germany
Prior art keywords
rna
reagents
extraction
blood leukocytes
wbcs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602005023133T
Other languages
English (en)
Inventor
Marianna Goldrick
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ambion Inc
Original Assignee
Ambion Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ambion Inc filed Critical Ambion Inc
Publication of DE602005023133D1 publication Critical patent/DE602005023133D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
DE602005023133T 2004-03-16 2005-03-16 Prozess und reagenzien für extraktion von rns von fraktionierten blutleukozyten Active DE602005023133D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/801,982 US20050208501A1 (en) 2004-03-16 2004-03-16 Process and reagents for extraction of RNA from fractionated blood leukocytes
PCT/US2005/008826 WO2005090984A1 (en) 2004-03-16 2005-03-16 Process and reagents for extraction of rna from fractionated blood leukocytes

Publications (1)

Publication Number Publication Date
DE602005023133D1 true DE602005023133D1 (de) 2010-10-07

Family

ID=34963160

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005023133T Active DE602005023133D1 (de) 2004-03-16 2005-03-16 Prozess und reagenzien für extraktion von rns von fraktionierten blutleukozyten

Country Status (6)

Country Link
US (1) US20050208501A1 (de)
EP (1) EP1728078B1 (de)
JP (2) JP2007529225A (de)
AT (1) ATE479095T1 (de)
DE (1) DE602005023133D1 (de)
WO (1) WO2005090984A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
WO2007100986A2 (en) * 2006-02-24 2007-09-07 Rosetta Inpharmatics Llc Extraction and diagnostic fluid devices, systems and methods of use
DE102006009469A1 (de) * 2006-02-27 2007-09-06 Alexakis, Antonis, Dr. med. dent. Antigenbildung gegen Viren, Krebszellen, sowie gegen Zellen die mit Viren infiziert sind durch RNA sowie DNA Extraktion
US20070207483A1 (en) * 2006-03-02 2007-09-06 Bio-Rad Laboratories, Inc. BUFFERS FOR DETECTION OF mRNA SEPARATED IN A MICROFLUIDIC DEVICE
ES2472765T3 (es) 2007-05-03 2014-07-03 F. Hoffmann-La Roche Ag Lisis y transcripción inversa mejoradas para la cuantificación de ARNm
WO2009009457A1 (en) * 2007-07-06 2009-01-15 University Of Louisville Research Foundation, Inc. Alzheimer's disease-specific micro-rna microarray and related methods
JP5367078B2 (ja) 2008-08-01 2013-12-11 エフ．ホフマン−ラ ロシュ アーゲー ｍＲＮＡ定量のための改善された溶解および逆転写
US20110027862A1 (en) * 2009-06-29 2011-02-03 Life Technologies Corporation Sample stabilization
US8774488B2 (en) 2010-03-11 2014-07-08 Cellscape Corporation Method and device for identification of nucleated red blood cells from a maternal blood sample
JPWO2011162326A1 (ja) * 2010-06-25 2013-08-22 株式会社日本遺伝子研究所 Ｒｎａ鎖の保存溶液
KR101420094B1 (ko) * 2010-10-27 2014-07-17 (주)바이오니아 다양한 생체시료분석을 위한 전자동실시간정량증폭장비, 다양한 생체시료분석을 위한 자동정제 및 반응준비 장치, 전자동 핵산정제 및 실시간 정량 유전자증폭 방법, 전자동 핵산정제방법, 실시간정량ｐｃｒ을 이용한 병원균의 전자동 생균수검사방법, 정량면역ｐｃｒ을 이용한 전자동 항원농도획득방법 및 타겟항원에 라벨링된 부착용 타겟핵산의 정제방법
DE102011080853B4 (de) 2011-08-11 2014-03-27 Axagarius Gmbh & Co. Kg Verfahren zur Isolierung von RNA aus Volblutproben
WO2015009970A1 (en) 2013-07-18 2015-01-22 Erythron Llc Spectroscopic measurements with parallel array detector
WO2015131151A2 (en) 2014-02-28 2015-09-03 Erythron, Llc Method and apparatus for determining markers of health by analysis of blood
DE102015220401B4 (de) * 2014-10-20 2022-12-29 Gen-Probe Incorporated Erythrozyten-Lyselösung
EP3282937A4 (de) 2015-04-14 2018-11-21 Nueon Inc. Verfahren und vorrichtung zur bestimmung von gesundheitsmarkern durch die analyse von blut
BR112018007474A2 (pt) * 2015-10-14 2018-10-30 Novozymes A/S ?limpeza de membranas de filtração de água?
US10000742B2 (en) 2015-11-19 2018-06-19 General Electric Company Device and method of collection for RNA viruses
WO2017165403A1 (en) * 2016-03-21 2017-09-28 Nueon Inc. Porous mesh spectrometry methods and apparatus
AU2017257978B2 (en) 2016-04-27 2022-12-22 Gen-Probe Incorporated Blood cell lysis reagent
CN109563539A (zh) 2016-06-15 2019-04-02 慕尼黑路德维希马克西米利安斯大学 使用dna纳米技术的单分子检测或定量
WO2018085699A1 (en) 2016-11-04 2018-05-11 Nueon Inc. Combination blood lancet and analyzer
CN109402238A (zh) * 2018-12-19 2019-03-01 广州海思医疗科技有限公司 用于血液、口腔拭子进行pcr的裂解液及试剂盒

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4652525A (en) * 1978-04-19 1987-03-24 The Regents Of The University Of California Recombinant bacterial plasmids containing the coding sequences of insulin genes
CA1301606C (en) * 1986-05-02 1992-05-26 David H. Gillespie Chaotropic method for evaluating nucleic acids in a biological sample
US4923620A (en) 1987-10-20 1990-05-08 Pall Corporation Device for depletion of the leukocyte content of blood and blood components
US4925572A (en) * 1987-10-20 1990-05-15 Pall Corporation Device and method for depletion of the leukocyte content of blood and blood components
US4880548A (en) 1988-02-17 1989-11-14 Pall Corporation Device and method for separating leucocytes from platelet concentrate
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5229012A (en) 1989-05-09 1993-07-20 Pall Corporation Method for depletion of the leucocyte content of blood and blood components
US5344561A (en) 1989-05-09 1994-09-06 Pall Corporation Device for depletion of the leucocyte content of blood and blood components
US5045449A (en) * 1989-06-14 1991-09-03 Thomas Jefferson University Methods of detecting a genetic predisposition for vascular aneurysms
JPH0825886B2 (ja) * 1989-07-14 1996-03-13 テルモ株式会社 白血球捕捉用フィルター材およびその製造方法
US5100564A (en) 1990-11-06 1992-03-31 Pall Corporation Blood collection and processing system
US5457024A (en) * 1993-01-22 1995-10-10 Aprogenex, Inc. Isolation of fetal erythrocytes
JPH08280384A (ja) * 1995-04-18 1996-10-29 Toyobo Co Ltd 有核細胞を含む試料から核酸または蛋白質を回収する方法
WO1996041810A1 (en) * 1995-06-08 1996-12-27 Progen Industries Limited Method and apparatus for dna extraction
US5702884A (en) * 1996-03-12 1997-12-30 Johnson & Johnson Clinical Diagnostics, Inc. Whole blood sample preparation for polymerase chain reaction using ammonium chloride and a carboxylic acid or metal carboxylate for selective red blood cell lysis
CA2286156C (en) 1997-04-08 2013-03-19 Pall Corporation Method of harvesting rare cells from blood products
US6204375B1 (en) * 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
GB9912631D0 (en) * 1999-05-28 1999-07-28 Nat Blood Authority The Cell counter
WO2000077253A1 (fr) * 1999-06-16 2000-12-21 Hitachi, Ltd. Appareil et procede d'examen des genes
DE60044977D1 (de) 1999-09-24 2010-10-28 Ambion Inc Cocktail von nukleaseinhibitoren
AU2001263836A1 (en) * 2000-04-11 2001-10-23 Bureco Ag Method of nucleic acid recovery
AU2001286499A1 (en) * 2000-08-16 2002-02-25 Matthew Baker Transfusion medicine leukodepletion filter devices as a source of genetic material for genotyping studies
US7108969B1 (en) * 2000-09-08 2006-09-19 Affymetrix, Inc. Methods for detecting and diagnosing oral cancer
AU2002247654B9 (en) * 2001-01-25 2007-05-10 Qiagen Gmbh Ammonium sulfate for neutralization of inhibitory effects
ATE454469T1 (de) 2002-01-28 2010-01-15 Ambion Inc Für den nachweis von nukleinsäuren kompetente biologische rohderivate
WO2003091407A2 (en) * 2002-04-24 2003-11-06 Hitachi Chemical Research Center, Inc. Device and method for high-throughput quantification of mrna from whole blood

Also Published As

Publication number Publication date
JP2011200236A (ja) 2011-10-13
EP1728078B1 (de) 2010-08-25
WO2005090984A1 (en) 2005-09-29
ATE479095T1 (de) 2010-09-15
US20050208501A1 (en) 2005-09-22
JP2007529225A (ja) 2007-10-25
EP1728078A1 (de) 2006-12-06

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