DE60138531D1 - Fluoreszierende im membran interkalierende proben und verfahren zu deren verwendung - Google Patents

Fluoreszierende im membran interkalierende proben und verfahren zu deren verwendung

Info

Publication number
DE60138531D1
DE60138531D1 DE60138531T DE60138531T DE60138531D1 DE 60138531 D1 DE60138531 D1 DE 60138531D1 DE 60138531 T DE60138531 T DE 60138531T DE 60138531 T DE60138531 T DE 60138531T DE 60138531 D1 DE60138531 D1 DE 60138531D1
Authority
DE
Germany
Prior art keywords
cells
dyes
intercaliating
samples
fluorescent membrane
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60138531T
Other languages
English (en)
Inventor
Brian D Gray
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Phanos Tech Inc
Original Assignee
Phanos Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phanos Tech Inc filed Critical Phanos Tech Inc
Application granted granted Critical
Publication of DE60138531D1 publication Critical patent/DE60138531D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
    • A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
    • A61K49/0032—Methine dyes, e.g. cyanine dyes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
    • C07D209/14—Radicals substituted by nitrogen atoms, not forming part of a nitro radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/56—Ring systems containing three or more rings
    • C07D209/58—[b]- or [c]-condensed
    • C07D209/60—Naphtho [b] pyrroles; Hydrogenated naphtho [b] pyrroles
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
    • C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
    • C07D215/12—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with substituted hydrocarbon radicals attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/0008—Methine or polymethine dyes, e.g. cyanine dyes substituted on the polymethine chain
    • C09B23/0016—Methine or polymethine dyes, e.g. cyanine dyes substituted on the polymethine chain the substituent being a halogen atom
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/0066—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain being part of a carbocyclic ring,(e.g. benzene, naphtalene, cyclohexene, cyclobutenene-quadratic acid)
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
    • C09B23/083—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines five >CH- groups
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
    • C09B23/086—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines more than five >CH- groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Indole Compounds (AREA)
  • Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)
DE60138531T 2000-03-03 2001-03-05 Fluoreszierende im membran interkalierende proben und verfahren zu deren verwendung Expired - Lifetime DE60138531D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18668200P 2000-03-03 2000-03-03
PCT/US2001/006923 WO2001066153A1 (en) 2000-03-03 2001-03-05 Fluorescent membrane intercalating probes and methods for their use

Publications (1)

Publication Number Publication Date
DE60138531D1 true DE60138531D1 (de) 2009-06-10

Family

ID=22685883

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60138531T Expired - Lifetime DE60138531D1 (de) 2000-03-03 2001-03-05 Fluoreszierende im membran interkalierende proben und verfahren zu deren verwendung

Country Status (8)

Country Link
US (2) US7462347B2 (de)
EP (1) EP1265640B1 (de)
JP (1) JP2003525915A (de)
AT (1) ATE429932T1 (de)
AU (2) AU2001245424B2 (de)
CA (1) CA2402352C (de)
DE (1) DE60138531D1 (de)
WO (1) WO2001066153A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001066153A1 (en) * 2000-03-03 2001-09-13 Phanos Technologies, Inc. Fluorescent membrane intercalating probes and methods for their use
AU2005234696B2 (en) * 2005-11-18 2012-02-16 Phanos Technologies, Inc. Fluorescent membrane intercalating probes and methods for their use
EP2892569B1 (de) * 2012-09-04 2018-12-12 Given Imaging Ltd. Luminale verabreichung von tag-molekülen für diagnostische anwendungen
JP6203846B2 (ja) * 2012-09-05 2017-09-27 ジュリア リウ， 腸細胞バリア機能不全を検出するための方法および組成物
US9034596B1 (en) 2014-04-17 2015-05-19 Kuwait University Method for fluorescent staining of cellular and intracellular membranes
CN104098500B (zh) * 2014-07-08 2016-03-16 中国科学院烟台海岸带研究所 基于花青的有机化合物及其应用
US10663473B2 (en) 2016-02-02 2020-05-26 Maximus Diagnostic Technologies Llc Method of identifying a status of a mucosal intestinal epithelial barrier to improve treatment of inflammatory bowel disease
WO2019094550A1 (en) * 2017-11-09 2019-05-16 Mindimmune Therapeutics, Inc. Dendritic cell recruitment from blood to brain in neurodegenerative disease
RU2665628C1 (ru) * 2018-01-25 2018-09-03 Федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр онкологии имени Н.Н. Блохина" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ онкологии им. Н.Н. Блохина" Минздрава России) Устройство для спектрально-флуоресцентного исследования содержания флуорохромов

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4859584A (en) * 1986-10-31 1989-08-22 Smithkline Beckman Corporation Cell growth rate determination by measurement of changes in cyanine dye levels in plasma membranes
US4762701A (en) * 1986-10-31 1988-08-09 Smithkline Beckman Corporation In vivo cellular tracking
US4783401A (en) * 1986-10-31 1988-11-08 Smithkline Beckman Corporation Viable cell labelling
DE68927479T2 (de) * 1988-05-02 1997-04-03 Phanos Tech Inc Verbindungen, zusammensetzungen und verfahren zum binden von bio-affektions-substanzen an oberflächenmembranen von bioteilchen
CN1093768C (zh) * 1995-01-30 2002-11-06 第一化学药品株式会社 诊断用标记物
JP3669752B2 (ja) * 1995-01-30 2005-07-13 第一化学薬品株式会社 診断用マーカー
US6004536A (en) * 1995-11-14 1999-12-21 Molecular Probes, Inc. Lipophilic cyanine dyes with enchanced aqueous solubilty
US5804389A (en) * 1995-12-29 1998-09-08 Phanos Technologies, Inc. Method for detecting abnormal epithelial cell shedding
US6027709A (en) * 1997-01-10 2000-02-22 Li-Cor Inc. Fluorescent cyanine dyes
WO2001066153A1 (en) * 2000-03-03 2001-09-13 Phanos Technologies, Inc. Fluorescent membrane intercalating probes and methods for their use

Also Published As

Publication number Publication date
JP2003525915A (ja) 2003-09-02
AU4542401A (en) 2001-09-17
US20060193780A1 (en) 2006-08-31
CA2402352C (en) 2012-08-28
US7462347B2 (en) 2008-12-09
EP1265640A4 (de) 2003-07-09
CA2402352A1 (en) 2001-09-13
US20100003196A1 (en) 2010-01-07
EP1265640A1 (de) 2002-12-18
WO2001066153A1 (en) 2001-09-13
AU2001245424B2 (en) 2005-08-18
ATE429932T1 (de) 2009-05-15
EP1265640B1 (de) 2009-04-29
US8029767B2 (en) 2011-10-04

Similar Documents

Publication Publication Date Title
WO2007061768A3 (en) Fluorescent membrane intercalating probes and methods for their use
DK1421211T3 (da) Fremgangsmåder til detektion af DNA, som stammer fra forskellige individer
ATE251658T1 (de) Wasserlösliche rhodaminfarbstoffe und ihre konjugate
Schopfer Cytochemical identification of arabinogalactan protein in the outer epidermal wall of maize coleoptiles
WO2003057851A3 (en) Binding proteins as biosensors
EP0969279A3 (de) Fluoreszenzpolarisationsverfahren bei mehreren Wellenlängen
DE60138531D1 (de) Fluoreszierende im membran interkalierende proben und verfahren zu deren verwendung
WO2006020947A9 (en) Phosphonate fluorescent dyes and conjugates
WO2007005222A8 (en) Cyanine dyes and methods of use in biological applications
ATE515575T1 (de) Verfahren und sonden für den nachweis von krebs
HK1026003A1 (en) Method of preparing a standard diagnostic gene transcript pattern.
WO2006025887A3 (en) Long wavelength thiol-reactive fluorophores
ATE397099T1 (de) Oligonukleotidprimer zur reversen transkription für den effizienten nachweis von hiv-1 und hiv-2 und methoden ihrer verwendung
DE60000014T2 (de) Linker-System zum Aktivieren von Oberflächen für die Biokonjugation und Verfahren zu ihrer Verwendung
DK1045918T3 (da) G protein-relaterede kinasemutanter i essentiel hypertension
WO2020033681A3 (en) Silicon-substituted rhodamine dyes and dye conjugates
MEP56408A (en) Conjugates and methods for the production thereof, and their use for transporting molecules via biological membranes
DE69808930D1 (de) Leberfunktionstest
WO2006060591A3 (en) Determination of cell viability and phenotype
Visser et al. Effect of pressure upon the fluorescence of various flavodoxins
CN108949154B (zh) 含萘酰亚胺的小分子作为荧光探针在rna检测和成像方面的应用
Felekyan et al. Filtered FCS and species cross correlation function
ATE394672T1 (de) Biosensoren sowie verfahren und kits zu deren anwendung
Mirończyk et al. Investigation of excited-state proton transfer in 2-naphthol derivatives containing a carboxyl group in organic solvents and in methanol–water mixtures
JP2007314444A (ja) 亜鉛蛍光プローブ

Legal Events

Date Code Title Description
8364 No opposition during term of opposition