ATE429932T1 - Fluoreszierende im membran interkalierende proben und verfahren zu deren verwendung - Google Patents

Fluoreszierende im membran interkalierende proben und verfahren zu deren verwendung

Info

Publication number
ATE429932T1
ATE429932T1 AT01918334T AT01918334T ATE429932T1 AT E429932 T1 ATE429932 T1 AT E429932T1 AT 01918334 T AT01918334 T AT 01918334T AT 01918334 T AT01918334 T AT 01918334T AT E429932 T1 ATE429932 T1 AT E429932T1
Authority
AT
Austria
Prior art keywords
cells
dyes
samples
fluorescent membrane
staining
Prior art date
Application number
AT01918334T
Other languages
English (en)
Inventor
Brian Gray
Original Assignee
Phanos Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phanos Tech Inc filed Critical Phanos Tech Inc
Application granted granted Critical
Publication of ATE429932T1 publication Critical patent/ATE429932T1/de

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
    • A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
    • A61K49/0032—Methine dyes, e.g. cyanine dyes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
    • C07D209/14—Radicals substituted by nitrogen atoms, not forming part of a nitro radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/56—Ring systems containing three or more rings
    • C07D209/58—[b]- or [c]-condensed
    • C07D209/60—Naphtho [b] pyrroles; Hydrogenated naphtho [b] pyrroles
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
    • C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
    • C07D215/12—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with substituted hydrocarbon radicals attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/0008—Methine or polymethine dyes, e.g. cyanine dyes substituted on the polymethine chain
    • C09B23/0016—Methine or polymethine dyes, e.g. cyanine dyes substituted on the polymethine chain the substituent being a halogen atom
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/0066—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain being part of a carbocyclic ring,(e.g. benzene, naphtalene, cyclohexene, cyclobutenene-quadratic acid)
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
    • C09B23/083—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines five >CH- groups
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
    • C09B23/086—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines more than five >CH- groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Biomedical Technology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Indole Compounds (AREA)
AT01918334T 2000-03-03 2001-03-05 Fluoreszierende im membran interkalierende proben und verfahren zu deren verwendung ATE429932T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18668200P 2000-03-03 2000-03-03
PCT/US2001/006923 WO2001066153A1 (en) 2000-03-03 2001-03-05 Fluorescent membrane intercalating probes and methods for their use

Publications (1)

Publication Number Publication Date
ATE429932T1 true ATE429932T1 (de) 2009-05-15

Family

ID=22685883

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01918334T ATE429932T1 (de) 2000-03-03 2001-03-05 Fluoreszierende im membran interkalierende proben und verfahren zu deren verwendung

Country Status (8)

Country Link
US (2) US7462347B2 (de)
EP (1) EP1265640B1 (de)
JP (1) JP2003525915A (de)
AT (1) ATE429932T1 (de)
AU (2) AU4542401A (de)
CA (1) CA2402352C (de)
DE (1) DE60138531D1 (de)
WO (1) WO2001066153A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE429932T1 (de) * 2000-03-03 2009-05-15 Phanos Tech Inc Fluoreszierende im membran interkalierende proben und verfahren zu deren verwendung
AU2005234696B2 (en) * 2005-11-18 2012-02-16 Phanos Technologies, Inc. Fluorescent membrane intercalating probes and methods for their use
US20150223675A1 (en) * 2012-09-04 2015-08-13 Given Imaging Ltd. Luminal administration of tag molecules for diagnostic applications
CA2901116C (en) * 2012-09-05 2021-11-09 Randall Thomas Irvin Methods and composition for detecting intestinal cell-barrier dysfunction
US9034596B1 (en) 2014-04-17 2015-05-19 Kuwait University Method for fluorescent staining of cellular and intracellular membranes
CN104098500B (zh) * 2014-07-08 2016-03-16 中国科学院烟台海岸带研究所 基于花青的有机化合物及其应用
US10663473B2 (en) 2016-02-02 2020-05-26 Maximus Diagnostic Technologies Llc Method of identifying a status of a mucosal intestinal epithelial barrier to improve treatment of inflammatory bowel disease
CN111315869A (zh) * 2017-11-09 2020-06-19 精神免疫疗法公司 神经退行性疾病中树突状细胞从血液募集到脑
RU2665628C1 (ru) * 2018-01-25 2018-09-03 Федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр онкологии имени Н.Н. Блохина" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ онкологии им. Н.Н. Блохина" Минздрава России) Устройство для спектрально-флуоресцентного исследования содержания флуорохромов

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4783401A (en) * 1986-10-31 1988-11-08 Smithkline Beckman Corporation Viable cell labelling
US4859584A (en) * 1986-10-31 1989-08-22 Smithkline Beckman Corporation Cell growth rate determination by measurement of changes in cyanine dye levels in plasma membranes
US4762701A (en) * 1986-10-31 1988-08-09 Smithkline Beckman Corporation In vivo cellular tracking
WO1989010758A1 (en) * 1988-05-02 1989-11-16 Zynaxis Technologies, Inc. Compounds, compositions and method for binding bio-affecting substances to surface membranes of bio-particles
CN1093768C (zh) * 1995-01-30 2002-11-06 第一化学药品株式会社 诊断用标记物
JP3669752B2 (ja) * 1995-01-30 2005-07-13 第一化学薬品株式会社 診断用マーカー
US6004536A (en) * 1995-11-14 1999-12-21 Molecular Probes, Inc. Lipophilic cyanine dyes with enchanced aqueous solubilty
US5804389A (en) * 1995-12-29 1998-09-08 Phanos Technologies, Inc. Method for detecting abnormal epithelial cell shedding
US6027709A (en) * 1997-01-10 2000-02-22 Li-Cor Inc. Fluorescent cyanine dyes
ATE429932T1 (de) * 2000-03-03 2009-05-15 Phanos Tech Inc Fluoreszierende im membran interkalierende proben und verfahren zu deren verwendung

Also Published As

Publication number Publication date
US20100003196A1 (en) 2010-01-07
CA2402352A1 (en) 2001-09-13
US8029767B2 (en) 2011-10-04
DE60138531D1 (de) 2009-06-10
EP1265640B1 (de) 2009-04-29
US20060193780A1 (en) 2006-08-31
AU4542401A (en) 2001-09-17
US7462347B2 (en) 2008-12-09
EP1265640A4 (de) 2003-07-09
CA2402352C (en) 2012-08-28
WO2001066153A1 (en) 2001-09-13
AU2001245424B2 (en) 2005-08-18
JP2003525915A (ja) 2003-09-02
EP1265640A1 (de) 2002-12-18

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