ATE429932T1 - FLUORESCENT MEMBRANE INTERCALATING SAMPLES AND METHOD FOR USE THEREOF - Google Patents

FLUORESCENT MEMBRANE INTERCALATING SAMPLES AND METHOD FOR USE THEREOF

Info

Publication number
ATE429932T1
ATE429932T1 AT01918334T AT01918334T ATE429932T1 AT E429932 T1 ATE429932 T1 AT E429932T1 AT 01918334 T AT01918334 T AT 01918334T AT 01918334 T AT01918334 T AT 01918334T AT E429932 T1 ATE429932 T1 AT E429932T1
Authority
AT
Austria
Prior art keywords
cells
dyes
samples
fluorescent membrane
staining
Prior art date
Application number
AT01918334T
Other languages
German (de)
Inventor
Brian Gray
Original Assignee
Phanos Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phanos Tech Inc filed Critical Phanos Tech Inc
Application granted granted Critical
Publication of ATE429932T1 publication Critical patent/ATE429932T1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
    • A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
    • A61K49/0032—Methine dyes, e.g. cyanine dyes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
    • C07D209/14—Radicals substituted by nitrogen atoms, not forming part of a nitro radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/56—Ring systems containing three or more rings
    • C07D209/58—[b]- or [c]-condensed
    • C07D209/60—Naphtho [b] pyrroles; Hydrogenated naphtho [b] pyrroles
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
    • C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
    • C07D215/12—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with substituted hydrocarbon radicals attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/0008—Methine or polymethine dyes, e.g. cyanine dyes substituted on the polymethine chain
    • C09B23/0016—Methine or polymethine dyes, e.g. cyanine dyes substituted on the polymethine chain the substituent being a halogen atom
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/0066—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain being part of a carbocyclic ring,(e.g. benzene, naphtalene, cyclohexene, cyclobutenene-quadratic acid)
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
    • C09B23/083—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines five >CH- groups
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
    • C09B23/086—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines more than five >CH- groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Indole Compounds (AREA)
  • Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)

Abstract

The invention relates to a family of cyanine dyes which fluoresce in the far red and near infra red wavelengths of the spectrum and preferably possess lipophilic side chains. The dyes of the invention are soluble in commercially available membrane staining vehicles, are useful as probes for rapidly staining lipophilic structures such as membranes in cells or isolated from cells, and are well retained therein. Methods of using the dyes to detect stained cells both in vivo and in vitro are also disclosed.
AT01918334T 2000-03-03 2001-03-05 FLUORESCENT MEMBRANE INTERCALATING SAMPLES AND METHOD FOR USE THEREOF ATE429932T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18668200P 2000-03-03 2000-03-03
PCT/US2001/006923 WO2001066153A1 (en) 2000-03-03 2001-03-05 Fluorescent membrane intercalating probes and methods for their use

Publications (1)

Publication Number Publication Date
ATE429932T1 true ATE429932T1 (en) 2009-05-15

Family

ID=22685883

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01918334T ATE429932T1 (en) 2000-03-03 2001-03-05 FLUORESCENT MEMBRANE INTERCALATING SAMPLES AND METHOD FOR USE THEREOF

Country Status (8)

Country Link
US (2) US7462347B2 (en)
EP (1) EP1265640B1 (en)
JP (1) JP2003525915A (en)
AT (1) ATE429932T1 (en)
AU (2) AU2001245424B2 (en)
CA (1) CA2402352C (en)
DE (1) DE60138531D1 (en)
WO (1) WO2001066153A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE429932T1 (en) * 2000-03-03 2009-05-15 Phanos Tech Inc FLUORESCENT MEMBRANE INTERCALATING SAMPLES AND METHOD FOR USE THEREOF
AU2005234696B2 (en) * 2005-11-18 2012-02-16 Phanos Technologies, Inc. Fluorescent membrane intercalating probes and methods for their use
EP2892569B1 (en) * 2012-09-04 2018-12-12 Given Imaging Ltd. Luminal administration of tag molecules for diagnostic applications
ES2665590T3 (en) * 2012-09-05 2018-04-26 Maximus Diagnostic Technologies Llc Methods and composition to detect intestinal cell barrier dysfunction
US9034596B1 (en) 2014-04-17 2015-05-19 Kuwait University Method for fluorescent staining of cellular and intracellular membranes
CN104098500B (en) * 2014-07-08 2016-03-16 中国科学院烟台海岸带研究所 Based on organic compound and the application thereof of cyanine
US10663473B2 (en) 2016-02-02 2020-05-26 Maximus Diagnostic Technologies Llc Method of identifying a status of a mucosal intestinal epithelial barrier to improve treatment of inflammatory bowel disease
EP3707239A4 (en) * 2017-11-09 2021-08-11 MindImmune Therapeutics, Inc. Dendritic cell recruitment from blood to brain in neurodegenerative disease
RU2665628C1 (en) * 2018-01-25 2018-09-03 Федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр онкологии имени Н.Н. Блохина" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ онкологии им. Н.Н. Блохина" Минздрава России) Device for spectral-fluorescence investigation of fluorochrome content

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4762701A (en) * 1986-10-31 1988-08-09 Smithkline Beckman Corporation In vivo cellular tracking
US4783401A (en) * 1986-10-31 1988-11-08 Smithkline Beckman Corporation Viable cell labelling
US4859584A (en) * 1986-10-31 1989-08-22 Smithkline Beckman Corporation Cell growth rate determination by measurement of changes in cyanine dye levels in plasma membranes
WO1989010758A1 (en) * 1988-05-02 1989-11-16 Zynaxis Technologies, Inc. Compounds, compositions and method for binding bio-affecting substances to surface membranes of bio-particles
JP3669752B2 (en) * 1995-01-30 2005-07-13 第一化学薬品株式会社 Diagnostic marker
KR100397020B1 (en) * 1995-01-30 2004-04-29 다이이치 가가쿠 야쿠힝 가부시키가이샤 Diagnostic marker
US6004536A (en) * 1995-11-14 1999-12-21 Molecular Probes, Inc. Lipophilic cyanine dyes with enchanced aqueous solubilty
US5804389A (en) * 1995-12-29 1998-09-08 Phanos Technologies, Inc. Method for detecting abnormal epithelial cell shedding
US6027709A (en) * 1997-01-10 2000-02-22 Li-Cor Inc. Fluorescent cyanine dyes
ATE429932T1 (en) * 2000-03-03 2009-05-15 Phanos Tech Inc FLUORESCENT MEMBRANE INTERCALATING SAMPLES AND METHOD FOR USE THEREOF

Also Published As

Publication number Publication date
AU4542401A (en) 2001-09-17
WO2001066153A1 (en) 2001-09-13
AU2001245424B2 (en) 2005-08-18
CA2402352C (en) 2012-08-28
CA2402352A1 (en) 2001-09-13
DE60138531D1 (en) 2009-06-10
US20100003196A1 (en) 2010-01-07
JP2003525915A (en) 2003-09-02
EP1265640A4 (en) 2003-07-09
US20060193780A1 (en) 2006-08-31
EP1265640A1 (en) 2002-12-18
US8029767B2 (en) 2011-10-04
EP1265640B1 (en) 2009-04-29
US7462347B2 (en) 2008-12-09

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