ATE429932T1 - FLUORESCENT MEMBRANE INTERCALATING SAMPLES AND METHOD FOR USE THEREOF - Google Patents
FLUORESCENT MEMBRANE INTERCALATING SAMPLES AND METHOD FOR USE THEREOFInfo
- Publication number
- ATE429932T1 ATE429932T1 AT01918334T AT01918334T ATE429932T1 AT E429932 T1 ATE429932 T1 AT E429932T1 AT 01918334 T AT01918334 T AT 01918334T AT 01918334 T AT01918334 T AT 01918334T AT E429932 T1 ATE429932 T1 AT E429932T1
- Authority
- AT
- Austria
- Prior art keywords
- cells
- dyes
- samples
- fluorescent membrane
- staining
- Prior art date
Links
- 239000012528 membrane Substances 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 238000009830 intercalation Methods 0.000 title 1
- 239000000975 dye Substances 0.000 abstract 3
- 238000010186 staining Methods 0.000 abstract 2
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- 230000000717 retained effect Effects 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 238000001228 spectrum Methods 0.000 abstract 1
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
- A61K49/0017—Fluorescence in vivo
- A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
- A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
- A61K49/0032—Methine dyes, e.g. cyanine dyes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/04—Indoles; Hydrogenated indoles
- C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
- C07D209/14—Radicals substituted by nitrogen atoms, not forming part of a nitro radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/56—Ring systems containing three or more rings
- C07D209/58—[b]- or [c]-condensed
- C07D209/60—Naphtho [b] pyrroles; Hydrogenated naphtho [b] pyrroles
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
- C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
- C07D215/12—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with substituted hydrocarbon radicals attached to ring carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/0008—Methine or polymethine dyes, e.g. cyanine dyes substituted on the polymethine chain
- C09B23/0016—Methine or polymethine dyes, e.g. cyanine dyes substituted on the polymethine chain the substituent being a halogen atom
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/0066—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain being part of a carbocyclic ring,(e.g. benzene, naphtalene, cyclohexene, cyclobutenene-quadratic acid)
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
- C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
- C09B23/083—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines five >CH- groups
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
- C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
- C09B23/086—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines more than five >CH- groups
Abstract
The invention relates to a family of cyanine dyes which fluoresce in the far red and near infra red wavelengths of the spectrum and preferably possess lipophilic side chains. The dyes of the invention are soluble in commercially available membrane staining vehicles, are useful as probes for rapidly staining lipophilic structures such as membranes in cells or isolated from cells, and are well retained therein. Methods of using the dyes to detect stained cells both in vivo and in vitro are also disclosed.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US18668200P | 2000-03-03 | 2000-03-03 | |
PCT/US2001/006923 WO2001066153A1 (en) | 2000-03-03 | 2001-03-05 | Fluorescent membrane intercalating probes and methods for their use |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE429932T1 true ATE429932T1 (en) | 2009-05-15 |
Family
ID=22685883
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT01918334T ATE429932T1 (en) | 2000-03-03 | 2001-03-05 | FLUORESCENT MEMBRANE INTERCALATING SAMPLES AND METHOD FOR USE THEREOF |
Country Status (8)
Country | Link |
---|---|
US (2) | US7462347B2 (en) |
EP (1) | EP1265640B1 (en) |
JP (1) | JP2003525915A (en) |
AT (1) | ATE429932T1 (en) |
AU (2) | AU4542401A (en) |
CA (1) | CA2402352C (en) |
DE (1) | DE60138531D1 (en) |
WO (1) | WO2001066153A1 (en) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001066153A1 (en) * | 2000-03-03 | 2001-09-13 | Phanos Technologies, Inc. | Fluorescent membrane intercalating probes and methods for their use |
AU2005234696B2 (en) | 2005-11-18 | 2012-02-16 | Phanos Technologies, Inc. | Fluorescent membrane intercalating probes and methods for their use |
US20150223675A1 (en) * | 2012-09-04 | 2015-08-13 | Given Imaging Ltd. | Luminal administration of tag molecules for diagnostic applications |
WO2014039699A1 (en) * | 2012-09-05 | 2014-03-13 | Arch Biopartners Inc. | Methods and composition for detecting intestinal cell-barrier dysfunction |
US9034596B1 (en) | 2014-04-17 | 2015-05-19 | Kuwait University | Method for fluorescent staining of cellular and intracellular membranes |
CN104098500B (en) * | 2014-07-08 | 2016-03-16 | 中国科学院烟台海岸带研究所 | Based on organic compound and the application thereof of cyanine |
EP4235173A3 (en) | 2016-02-02 | 2023-09-13 | Maximus Diagnostic Technologies LLC | Assessment of intestinal barrier function to improve treatment of inflammatory bowel disease |
CN111315869A (en) * | 2017-11-09 | 2020-06-19 | 精神免疫疗法公司 | Dendritic cell recruitment from blood to brain in neurodegenerative diseases |
RU2665628C1 (en) * | 2018-01-25 | 2018-09-03 | Федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр онкологии имени Н.Н. Блохина" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ онкологии им. Н.Н. Блохина" Минздрава России) | Device for spectral-fluorescence investigation of fluorochrome content |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4859584A (en) * | 1986-10-31 | 1989-08-22 | Smithkline Beckman Corporation | Cell growth rate determination by measurement of changes in cyanine dye levels in plasma membranes |
US4762701A (en) * | 1986-10-31 | 1988-08-09 | Smithkline Beckman Corporation | In vivo cellular tracking |
US4783401A (en) * | 1986-10-31 | 1988-11-08 | Smithkline Beckman Corporation | Viable cell labelling |
JP3038339B2 (en) * | 1988-05-02 | 2000-05-08 | ザイナクシス・テクノロジーズ・インコーポレーテッド | Compounds that bind bioaffecting substances to the surface membrane of bioparticles |
JP3669752B2 (en) * | 1995-01-30 | 2005-07-13 | 第一化学薬品株式会社 | Diagnostic marker |
CA2211470A1 (en) * | 1995-01-30 | 1996-08-08 | Daiichi Pure Chemicals Co., Ltd. | Diagnostic marker |
US6004536A (en) * | 1995-11-14 | 1999-12-21 | Molecular Probes, Inc. | Lipophilic cyanine dyes with enchanced aqueous solubilty |
US5804389A (en) * | 1995-12-29 | 1998-09-08 | Phanos Technologies, Inc. | Method for detecting abnormal epithelial cell shedding |
US6027709A (en) * | 1997-01-10 | 2000-02-22 | Li-Cor Inc. | Fluorescent cyanine dyes |
WO2001066153A1 (en) * | 2000-03-03 | 2001-09-13 | Phanos Technologies, Inc. | Fluorescent membrane intercalating probes and methods for their use |
-
2001
- 2001-03-05 WO PCT/US2001/006923 patent/WO2001066153A1/en active IP Right Grant
- 2001-03-05 EP EP01918334A patent/EP1265640B1/en not_active Expired - Lifetime
- 2001-03-05 DE DE60138531T patent/DE60138531D1/en not_active Expired - Lifetime
- 2001-03-05 AU AU4542401A patent/AU4542401A/en active Pending
- 2001-03-05 AU AU2001245424A patent/AU2001245424B2/en not_active Expired
- 2001-03-05 JP JP2001564805A patent/JP2003525915A/en active Pending
- 2001-03-05 CA CA2402352A patent/CA2402352C/en not_active Expired - Lifetime
- 2001-03-05 AT AT01918334T patent/ATE429932T1/en not_active IP Right Cessation
-
2006
- 2006-02-03 US US11/346,554 patent/US7462347B2/en not_active Expired - Lifetime
-
2008
- 2008-12-08 US US12/330,073 patent/US8029767B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
AU2001245424B2 (en) | 2005-08-18 |
EP1265640A4 (en) | 2003-07-09 |
EP1265640A1 (en) | 2002-12-18 |
US20100003196A1 (en) | 2010-01-07 |
CA2402352C (en) | 2012-08-28 |
CA2402352A1 (en) | 2001-09-13 |
AU4542401A (en) | 2001-09-17 |
WO2001066153A1 (en) | 2001-09-13 |
DE60138531D1 (en) | 2009-06-10 |
US8029767B2 (en) | 2011-10-04 |
JP2003525915A (en) | 2003-09-02 |
US20060193780A1 (en) | 2006-08-31 |
US7462347B2 (en) | 2008-12-09 |
EP1265640B1 (en) | 2009-04-29 |
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Legal Events
Date | Code | Title | Description |
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RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |