DE60042370D1 - Detektion von nukleinsäuren - Google Patents
Detektion von nukleinsäurenInfo
- Publication number
- DE60042370D1 DE60042370D1 DE60042370T DE60042370T DE60042370D1 DE 60042370 D1 DE60042370 D1 DE 60042370D1 DE 60042370 T DE60042370 T DE 60042370T DE 60042370 T DE60042370 T DE 60042370T DE 60042370 D1 DE60042370 D1 DE 60042370D1
- Authority
- DE
- Germany
- Prior art keywords
- target nucleic
- nucleic acids
- immobilized
- substrate
- determined
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 108020004707 nucleic acids Proteins 0.000 title abstract 5
- 150000007523 nucleic acids Chemical class 0.000 title abstract 5
- 102000039446 nucleic acids Human genes 0.000 title abstract 5
- 238000001514 detection method Methods 0.000 title abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- 239000002245 particle Substances 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 108020005187 Oligonucleotide Probes Proteins 0.000 abstract 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
- 238000000149 argon plasma sintering Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000007837 multiplex assay Methods 0.000 abstract 1
- 239000002751 oligonucleotide probe Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Nanotechnology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17279899P | 1999-12-21 | 1999-12-21 | |
| PCT/US2000/034726 WO2001046474A1 (en) | 1999-12-21 | 2000-12-21 | Detection of nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60042370D1 true DE60042370D1 (de) | 2009-07-23 |
Family
ID=22629292
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60042370T Expired - Fee Related DE60042370D1 (de) | 1999-12-21 | 2000-12-21 | Detektion von nukleinsäuren |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP1242626B1 (de) |
| JP (1) | JP2003517842A (de) |
| KR (1) | KR20020070328A (de) |
| CN (1) | CN1312287C (de) |
| AT (1) | ATE433499T1 (de) |
| AU (1) | AU2586001A (de) |
| BR (1) | BR0016621A (de) |
| CA (1) | CA2394974A1 (de) |
| DE (1) | DE60042370D1 (de) |
| MX (1) | MXPA02006095A (de) |
| WO (1) | WO2001046474A1 (de) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7829025B2 (en) | 2001-03-28 | 2010-11-09 | Venture Lending & Leasing Iv, Inc. | Systems and methods for thermal actuation of microfluidic devices |
| DE10155053B4 (de) * | 2001-11-09 | 2005-11-24 | Friz Biochem Gesellschaft Für Bioanalytik Mbh | Reversible Bindung eines Fluorophors an eine Oberfläche zur Detektion von Ligat-Ligand-Assoziationsereignissen durch Fluoreszenz-Quenchen |
| KR20040035248A (ko) * | 2002-10-19 | 2004-04-29 | 제노프라 주식회사 | 단일가닥핵산 어레이를 이용한 특정물질 동정 및 분석방법및 대장균에 대한 핵산 리간드 |
| EP2402089A1 (de) | 2003-07-31 | 2012-01-04 | Handylab, Inc. | Verarbeitung partikelhaltiger Proben |
| US8852862B2 (en) | 2004-05-03 | 2014-10-07 | Handylab, Inc. | Method for processing polynucleotide-containing samples |
| EP1790706A1 (de) * | 2004-06-22 | 2007-05-30 | FUJIFILM Corporation | Fluoreszierendes material, fluoreszierende materialzusammensetzung und verfahren zur fluoreszenzdetektion |
| US7998708B2 (en) | 2006-03-24 | 2011-08-16 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
| ES2692380T3 (es) | 2006-03-24 | 2018-12-03 | Handylab, Inc. | Método para realizar PCR con un cartucho con varias pistas |
| US8883490B2 (en) | 2006-03-24 | 2014-11-11 | Handylab, Inc. | Fluorescence detector for microfluidic diagnostic system |
| US10900066B2 (en) | 2006-03-24 | 2021-01-26 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
| US11806718B2 (en) | 2006-03-24 | 2023-11-07 | Handylab, Inc. | Fluorescence detector for microfluidic diagnostic system |
| WO2008061165A2 (en) | 2006-11-14 | 2008-05-22 | Handylab, Inc. | Microfluidic cartridge and method of making same |
| US8287820B2 (en) | 2007-07-13 | 2012-10-16 | Handylab, Inc. | Automated pipetting apparatus having a combined liquid pump and pipette head system |
| US8182763B2 (en) | 2007-07-13 | 2012-05-22 | Handylab, Inc. | Rack for sample tubes and reagent holders |
| US8105783B2 (en) | 2007-07-13 | 2012-01-31 | Handylab, Inc. | Microfluidic cartridge |
| US9186677B2 (en) | 2007-07-13 | 2015-11-17 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
| WO2009012185A1 (en) | 2007-07-13 | 2009-01-22 | Handylab, Inc. | Polynucleotide capture materials, and methods of using same |
| KR101465961B1 (ko) * | 2007-10-09 | 2014-12-01 | 삼성전자주식회사 | 유전자 검출 방법 및 장치 |
| US8409802B2 (en) * | 2009-08-14 | 2013-04-02 | Roche Molecular Systems, Inc. | Format of probes to detect nucleic acid differences |
| EP2534463B1 (de) * | 2010-02-11 | 2020-01-08 | ChemoMetec A/S | Verfahren zur analyse von zellulärem dna-gehalt |
| CN106148512B (zh) | 2011-04-15 | 2020-07-10 | 贝克顿·迪金森公司 | 扫描实时微流体热循环仪和用于同步的热循环和扫描光学检测的方法 |
| CN104040238B (zh) | 2011-11-04 | 2017-06-27 | 汉迪拉布公司 | 多核苷酸样品制备装置 |
| EP2798350B1 (de) * | 2011-12-29 | 2021-07-28 | Sight Diagnostics Ltd. | Verfahren und systeme zur erkennung eines krankheitserregers in einer biologischen probe |
| JP6262152B2 (ja) | 2012-02-03 | 2018-01-17 | ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ | 分子診断試験の分布及び試験間のコンパチビリティ判断のための外部ファイル |
| US9428792B2 (en) | 2013-03-14 | 2016-08-30 | Certirx Corporation | Nucleic acid-based authentication and identification codes |
| CN108351349B (zh) * | 2015-09-10 | 2020-09-29 | 因斯利克萨公司 | 用于多路定量核酸扩增的方法和系统 |
| CN105505755A (zh) * | 2015-12-23 | 2016-04-20 | 杭州谷禾信息技术有限公司 | 空间转录组建库测序方法及所用装置 |
| CN107573331B (zh) * | 2017-04-25 | 2019-11-15 | 浙江大学 | 能够提高寡核苷酸链Tm值的标记化合物及其应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5962218A (en) * | 1988-11-03 | 1999-10-05 | Igen International Inc. | Methods and apparatus for improved luminescence assays |
| US5049490A (en) | 1990-02-20 | 1991-09-17 | Eastman Kodak Co. | Quantitative determination of a DNA polymerase and a test kit useful in same |
| US5670316A (en) * | 1990-05-07 | 1997-09-23 | Daikin Industries, Ltd. | Diagnostic applications of double D-loop formation |
| US5837832A (en) * | 1993-06-25 | 1998-11-17 | Affymetrix, Inc. | Arrays of nucleic acid probes on biological chips |
| EP1411047A1 (de) | 1993-06-30 | 2004-04-21 | Abbott Laboratories | Interkalatoren mit Affinität für DNA und ihre Anwendung |
| US5925517A (en) * | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| ATE280243T1 (de) | 1994-04-29 | 2004-11-15 | Johnson & Johnson Clin Diag | Homogenes verfahren zum nachweis von doppelstrang-nukleinsäuren mittels fluoreszierender farbstoffe und dafür nützliche kits |
| US5695934A (en) * | 1994-10-13 | 1997-12-09 | Lynx Therapeutics, Inc. | Massively parallel sequencing of sorted polynucleotides |
| EP1818417B1 (de) * | 1996-07-29 | 2014-02-12 | Nanosphere, Inc. | Nanopartikel mit angehängten Oligonukleotiden und ihre Verwendungen |
| US5935791A (en) * | 1997-09-23 | 1999-08-10 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| US5989823A (en) | 1998-09-18 | 1999-11-23 | Nexstar Pharmaceuticals, Inc. | Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction |
| US6232066B1 (en) * | 1997-12-19 | 2001-05-15 | Neogen, Inc. | High throughput assay system |
-
2000
- 2000-12-21 DE DE60042370T patent/DE60042370D1/de not_active Expired - Fee Related
- 2000-12-21 EP EP00989349A patent/EP1242626B1/de not_active Expired - Lifetime
- 2000-12-21 BR BR0016621-9A patent/BR0016621A/pt not_active Application Discontinuation
- 2000-12-21 MX MXPA02006095A patent/MXPA02006095A/es unknown
- 2000-12-21 KR KR1020027008105A patent/KR20020070328A/ko not_active Application Discontinuation
- 2000-12-21 AU AU25860/01A patent/AU2586001A/en not_active Abandoned
- 2000-12-21 JP JP2001546969A patent/JP2003517842A/ja not_active Abandoned
- 2000-12-21 CN CNB008189811A patent/CN1312287C/zh not_active Expired - Fee Related
- 2000-12-21 CA CA002394974A patent/CA2394974A1/en not_active Abandoned
- 2000-12-21 WO PCT/US2000/034726 patent/WO2001046474A1/en active Application Filing
- 2000-12-21 AT AT00989349T patent/ATE433499T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| BR0016621A (pt) | 2002-09-03 |
| KR20020070328A (ko) | 2002-09-05 |
| JP2003517842A (ja) | 2003-06-03 |
| CA2394974A1 (en) | 2001-06-28 |
| MXPA02006095A (es) | 2002-12-13 |
| ATE433499T1 (de) | 2009-06-15 |
| EP1242626B1 (de) | 2009-06-10 |
| WO2001046474A1 (en) | 2001-06-28 |
| EP1242626A1 (de) | 2002-09-25 |
| WO2001046474A8 (en) | 2001-11-08 |
| CN1312287C (zh) | 2007-04-25 |
| AU2586001A (en) | 2001-07-03 |
| CN1434874A (zh) | 2003-08-06 |
| EP1242626A4 (de) | 2005-03-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |