DE39743T1 - Rekombinante plasmidringe mit breitem wirtspektrum als klonierungsvektor und ihre herstellung. - Google Patents
Rekombinante plasmidringe mit breitem wirtspektrum als klonierungsvektor und ihre herstellung.Info
- Publication number
- DE39743T1 DE39743T1 DE198080103658T DE80103658T DE39743T1 DE 39743 T1 DE39743 T1 DE 39743T1 DE 198080103658 T DE198080103658 T DE 198080103658T DE 80103658 T DE80103658 T DE 80103658T DE 39743 T1 DE39743 T1 DE 39743T1
- Authority
- DE
- Germany
- Prior art keywords
- plasmid
- fragment
- bacterial
- daltons
- ring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000013612 plasmid Substances 0.000 title claims 95
- 238000004519 manufacturing process Methods 0.000 title claims 6
- 239000013599 cloning vector Substances 0.000 title 1
- 239000012634 fragment Substances 0.000 claims 31
- 230000001580 bacterial effect Effects 0.000 claims 16
- 108020004414 DNA Proteins 0.000 claims 15
- 238000000034 method Methods 0.000 claims 15
- 102000053602 DNA Human genes 0.000 claims 12
- 230000003115 biocidal effect Effects 0.000 claims 11
- 241000894006 Bacteria Species 0.000 claims 9
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims 9
- 230000015556 catabolic process Effects 0.000 claims 9
- 238000006731 degradation reaction Methods 0.000 claims 9
- 108091008146 restriction endonucleases Proteins 0.000 claims 9
- 210000004027 cell Anatomy 0.000 claims 7
- 238000004220 aggregation Methods 0.000 claims 6
- 230000002776 aggregation Effects 0.000 claims 6
- FPPNZSSZRUTDAP-UWFZAAFLSA-N carbenicillin Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)C(C(O)=O)C1=CC=CC=C1 FPPNZSSZRUTDAP-UWFZAAFLSA-N 0.000 claims 6
- 229960003669 carbenicillin Drugs 0.000 claims 6
- 230000010076 replication Effects 0.000 claims 6
- 229940024606 amino acid Drugs 0.000 claims 4
- 150000001413 amino acids Chemical class 0.000 claims 4
- 239000003242 anti bacterial agent Substances 0.000 claims 3
- 229940088710 antibiotic agent Drugs 0.000 claims 3
- 230000032823 cell division Effects 0.000 claims 3
- 238000000338 in vitro Methods 0.000 claims 3
- 238000001727 in vivo Methods 0.000 claims 3
- 101100112724 Prochlorococcus marinus (strain SARG / CCMP1375 / SS120) ccsB gene Proteins 0.000 claims 2
- 239000004098 Tetracycline Substances 0.000 claims 2
- 230000005540 biological transmission Effects 0.000 claims 2
- 239000003550 marker Substances 0.000 claims 2
- 238000002360 preparation method Methods 0.000 claims 2
- 229960002180 tetracycline Drugs 0.000 claims 2
- 229930101283 tetracycline Natural products 0.000 claims 2
- 235000019364 tetracycline Nutrition 0.000 claims 2
- 150000003522 tetracyclines Chemical class 0.000 claims 2
- 230000009466 transformation Effects 0.000 claims 2
- 230000017105 transposition Effects 0.000 claims 2
- 241000589151 Azotobacter Species 0.000 claims 1
- 230000004543 DNA replication Effects 0.000 claims 1
- 108010042407 Endonucleases Proteins 0.000 claims 1
- 102000004533 Endonucleases Human genes 0.000 claims 1
- 241000588722 Escherichia Species 0.000 claims 1
- FFEARJCKVFRZRR-UHFFFAOYSA-N L-Methionine Natural products CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 claims 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims 1
- 229930182844 L-isoleucine Natural products 0.000 claims 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims 1
- 229930195722 L-methionine Natural products 0.000 claims 1
- 102000003960 Ligases Human genes 0.000 claims 1
- 108090000364 Ligases Proteins 0.000 claims 1
- 101710163270 Nuclease Proteins 0.000 claims 1
- 241000589516 Pseudomonas Species 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 150000001768 cations Chemical class 0.000 claims 1
- 230000002759 chromosomal effect Effects 0.000 claims 1
- 210000000349 chromosome Anatomy 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 230000021615 conjugation Effects 0.000 claims 1
- 230000029087 digestion Effects 0.000 claims 1
- 230000002068 genetic effect Effects 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 229960000310 isoleucine Drugs 0.000 claims 1
- 229960004452 methionine Drugs 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 230000026683 transduction Effects 0.000 claims 1
- 238000010361 transduction Methods 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/12—Methionine; Cysteine; Cystine
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/147,563 US4374200A (en) | 1980-05-08 | 1980-05-08 | Broad host range small plasmid rings as cloning vehicles |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE39743T1 true DE39743T1 (de) | 1983-02-03 |
Family
ID=22522070
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE198080103658T Pending DE39743T1 (de) | 1980-05-08 | 1980-06-27 | Rekombinante plasmidringe mit breitem wirtspektrum als klonierungsvektor und ihre herstellung. |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US4374200A (OSRAM) |
| EP (1) | EP0039743A3 (OSRAM) |
| JP (2) | JPS572299A (OSRAM) |
| AU (1) | AU538600B2 (OSRAM) |
| CA (1) | CA1150169A (OSRAM) |
| DE (1) | DE39743T1 (OSRAM) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4508823A (en) * | 1980-05-08 | 1985-04-02 | Microlife Technics, Inc. | Gene splicing method and products produced therefrom |
| US4508824A (en) * | 1980-05-08 | 1985-04-02 | Microlife Technics, Inc. | Pseudomonas degradation of hydrocarbons |
| US4508827A (en) * | 1980-05-08 | 1985-04-02 | Microlife Technics, Inc. | Molecular cloning vectors for use in gram-negative bacteria |
| JPS58893A (ja) * | 1981-06-25 | 1983-01-06 | Ajinomoto Co Inc | 発酵法によるｌ−イソロイシンの製造法 |
| US4654307A (en) * | 1983-02-17 | 1987-03-31 | The Research Foundation Of State University Of New York | Novel bacteria containing a plasmid having a tRNA code |
| US4567141A (en) * | 1983-04-25 | 1986-01-28 | Microlife Technics, Inc. | Plasmid vectors including Tn904 |
| US4593003A (en) * | 1983-05-19 | 1986-06-03 | Microlife Technics, Inc. | Bacterial method and compositions for isoprenoid degradation |
| DE3585275D1 (de) | 1984-12-10 | 1992-03-05 | Monsanto Co | Einsetzung des bacillus thuringiensis kristallproteingens in pflanzen kolonisierende mikroorganismen und deren verwendung. |
| US4695455A (en) * | 1985-01-22 | 1987-09-22 | Mycogen Corporation | Cellular encapsulation of pesticides produced by expression of heterologous genes |
| EP0200708B1 (en) * | 1985-04-30 | 1992-12-30 | Monsanto Company | Plant-colonizing microorganisms containing the toxin gene of b. thuringiensis as a chromosomal insertion |
| JPS63104406U (OSRAM) * | 1986-12-26 | 1988-07-06 | ||
| CN102016045A (zh) | 2007-05-02 | 2011-04-13 | 梅瑞尔有限公司 | 具有改善的表达和稳定性的dna质粒 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4237224A (en) * | 1974-11-04 | 1980-12-02 | Board Of Trustees Of The Leland Stanford Jr. University | Process for producing biologically functional molecular chimeras |
-
1980
- 1980-05-08 US US06/147,563 patent/US4374200A/en not_active Expired - Lifetime
- 1980-06-18 AU AU59374/80A patent/AU538600B2/en not_active Ceased
- 1980-06-27 EP EP80103658A patent/EP0039743A3/en not_active Withdrawn
- 1980-06-27 DE DE198080103658T patent/DE39743T1/de active Pending
- 1980-06-30 CA CA000355182A patent/CA1150169A/en not_active Expired
- 1980-10-07 JP JP13943180A patent/JPS572299A/ja active Granted
-
1986
- 1986-01-17 JP JP61006630A patent/JPS61234784A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS61234784A (ja) | 1986-10-20 |
| JPH0158949B2 (OSRAM) | 1989-12-14 |
| AU5937480A (en) | 1981-11-12 |
| EP0039743A3 (en) | 1982-10-27 |
| AU538600B2 (en) | 1984-08-23 |
| US4374200A (en) | 1983-02-15 |
| JPS6359677B2 (OSRAM) | 1988-11-21 |
| JPS572299A (en) | 1982-01-07 |
| EP0039743A2 (en) | 1981-11-18 |
| CA1150169A (en) | 1983-07-19 |
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