DE3855591D1 - Verfahren zur Herstellung eines biologisch-aktiven Peptides durch Expression von modifizierten Speicherproteingenen in transgenetischen Pflanzen - Google Patents

Verfahren zur Herstellung eines biologisch-aktiven Peptides durch Expression von modifizierten Speicherproteingenen in transgenetischen Pflanzen

Info

Publication number
DE3855591D1
DE3855591D1 DE3855591T DE3855591T DE3855591D1 DE 3855591 D1 DE3855591 D1 DE 3855591D1 DE 3855591 T DE3855591 T DE 3855591T DE 3855591 T DE3855591 T DE 3855591T DE 3855591 D1 DE3855591 D1 DE 3855591D1
Authority
DE
Germany
Prior art keywords
expression
production
biologically active
storage protein
transgenic plants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE3855591T
Other languages
English (en)
Other versions
DE3855591T2 (de
Inventor
Joel Stefaan Vandekerckhove
Enno Krebbers
Johan Botterman
Jan Leemans
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer CropScience NV
Original Assignee
Plant Genetic Systems NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plant Genetic Systems NV filed Critical Plant Genetic Systems NV
Application granted granted Critical
Publication of DE3855591D1 publication Critical patent/DE3855591D1/de
Publication of DE3855591T2 publication Critical patent/DE3855591T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8253—Methionine or cysteine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
DE3855591T 1987-10-20 1988-10-20 Verfahren zur Herstellung eines biologisch-aktiven Peptides durch Expression von modifizierten Speicherproteingenen in transgenetischen Pflanzen Expired - Fee Related DE3855591T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP87402348 1987-10-20

Publications (2)

Publication Number Publication Date
DE3855591D1 true DE3855591D1 (de) 1996-11-07
DE3855591T2 DE3855591T2 (de) 1997-04-03

Family

ID=8198266

Family Applications (2)

Application Number Title Priority Date Filing Date
DE3856589T Expired - Lifetime DE3856589D1 (de) 1987-10-20 1988-10-20 5'-flankierende Region eines Genes von Arabidopsis, das für einen 2S Albumin-Prekursor kodiert
DE3855591T Expired - Fee Related DE3855591T2 (de) 1987-10-20 1988-10-20 Verfahren zur Herstellung eines biologisch-aktiven Peptides durch Expression von modifizierten Speicherproteingenen in transgenetischen Pflanzen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE3856589T Expired - Lifetime DE3856589D1 (de) 1987-10-20 1988-10-20 5'-flankierende Region eines Genes von Arabidopsis, das für einen 2S Albumin-Prekursor kodiert

Country Status (4)

Country Link
US (1) US5623067A (de)
JP (1) JP3232068B2 (de)
AT (2) ATE143694T1 (de)
DE (2) DE3856589D1 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5550318A (en) * 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6803499B1 (en) 1989-08-09 2004-10-12 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6777589B1 (en) * 1990-01-22 2004-08-17 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6025545A (en) * 1990-01-22 2000-02-15 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
CA2074355C (en) * 1990-01-22 2008-10-28 Ronald C. Lundquist Method of producing fertile transgenic corn plants
US6329574B1 (en) 1990-01-22 2001-12-11 Dekalb Genetics Corporation High lysine fertile transgenic corn plants
US6946587B1 (en) * 1990-01-22 2005-09-20 Dekalb Genetics Corporation Method for preparing fertile transgenic corn plants
US6395966B1 (en) * 1990-08-09 2002-05-28 Dekalb Genetics Corp. Fertile transgenic maize plants containing a gene encoding the pat protein
US6281411B1 (en) 1993-08-25 2001-08-28 Dekalb Genetics Corporation Transgenic monocots plants with increased glycine-betaine content
US6118047A (en) 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
US6326527B1 (en) * 1993-08-25 2001-12-04 Dekalb Genetics Corporation Method for altering the nutritional content of plant seed
US6100450A (en) * 1997-10-22 2000-08-08 Rhone-Poulenc Agrochimie Seed specific promoters based on arabidopsis genes
WO2000012733A1 (en) * 1998-08-28 2000-03-09 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from end genes
US6784342B1 (en) 1999-08-04 2004-08-31 The University Of British Columbia Regulation of embryonic transcription in plants
US6403862B1 (en) * 1999-09-24 2002-06-11 Pioneer Hi-Bred International, Inc. Seed-preferred promoter from maize
CA2409876C (en) * 2000-05-24 2012-01-03 The University Of British Columbia Gene regulatory region that promotes early seed-specific transcription
AU8847801A (en) 2000-08-25 2002-03-04 Basf Plant Science Gmbh Plant polynucleotides encoding novel prenyl proteases
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
US7250296B2 (en) * 2003-03-05 2007-07-31 University Of Florida Research Foundation, Inc. Nucleotide sequences of 2S albumin gene and its promoter from grape and uses thereof
EP2256189A1 (de) 2003-03-28 2010-12-01 National Institute Of Agrobiological Sciences Verfahren zur Herstellung eines pflanzlichen Speicherorgans mit hoher rekombinant GLP-1 Derivat Produktion
EP1655364A3 (de) * 2004-11-05 2006-08-02 BASF Plant Science GmbH Expressionskassetten zur bevorzugten Expression in Pflanzensamen
CA2523471A1 (en) * 2004-11-05 2006-05-05 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
AU2006249983B2 (en) 2005-05-23 2011-03-17 Arcadia Biosciences, Inc. Safflower with elevated gamma-linolenic acid
WO2006125749A2 (en) * 2005-05-25 2006-11-30 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
WO2006136579A2 (en) * 2005-06-23 2006-12-28 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
US8653325B2 (en) * 2005-12-15 2014-02-18 Targeted Growth, Inc. Increased seed size and seed number through transgenic over expression of revoluta protein during early embryo development
ES2405546T3 (es) 2007-09-04 2013-05-31 Basf Se Copolímeros, formulaciones acuosas de los mismos y usos de las mismas
WO2012142106A1 (en) 2011-04-11 2012-10-18 Targeted Growth, Inc. Identification and the use of krp mutants in plants

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE240911C (de) *
GB2068971B (en) * 1980-01-30 1983-06-08 Searle & Co Recombinant dna techniques
CA1192510A (en) * 1981-05-27 1985-08-27 Lawrence E. Pelcher Rna plant virus vector or portion thereof, a method of construction thereof, and a method of producing a gene derived product therefrom
WO1983001176A1 (en) * 1981-10-01 1983-04-14 Int Plant Research Inst Process for the genetic modification of cereals with transformation vectors
US4617149A (en) * 1983-09-21 1986-10-14 Eli Lilly And Company Growth hormone release factor analogs
BR8404834A (pt) * 1983-09-26 1985-08-13 Agrigenetics Res Ass Metodo para modificar geneticamente uma celula vegetal
EP0208418B1 (de) * 1985-06-12 1991-10-30 Lubrizol Genetics Inc. Modifiziertes Zein
US4886878A (en) * 1985-06-12 1989-12-12 Lubrizol Genetics, Inc. Modified zein genes containing lysine
US4956282A (en) * 1985-07-29 1990-09-11 Calgene, Inc. Mammalian peptide expression in plant cells
JPH01500718A (ja) * 1986-05-29 1989-03-16 カルジーン，インコーポレイティド ブラッシカの種における形質転換及び外来遺伝子の発現
NZ221259A (en) * 1986-07-31 1990-05-28 Calgene Inc Seed specific transcriptional regulation
US5003045A (en) * 1986-08-29 1991-03-26 Lubrizol Genetics, Inc. Modified 7S legume seed storage proteins

Also Published As

Publication number Publication date
JP2000106890A (ja) 2000-04-18
DE3855591T2 (de) 1997-04-03
DE3856589D1 (de) 2006-04-06
ATE316144T1 (de) 2006-02-15
ATE143694T1 (de) 1996-10-15
JP3232068B2 (ja) 2001-11-26
US5623067A (en) 1997-04-22

Similar Documents

Publication Publication Date Title
DE3855591D1 (de) Verfahren zur Herstellung eines biologisch-aktiven Peptides durch Expression von modifizierten Speicherproteingenen in transgenetischen Pflanzen
AU2811889A (en) A process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants
ATE112135T1 (de) Überexpression von phytochrom in transgenen pflanzen.
ATE484189T1 (de) Stressbedingte transkriptionsfaktorproteine und verwendungsverfahren für pflanzen
DE69210645T2 (de) Verfahren zur Herstellung von cysteinfreien Peptiden
ATE195218T1 (de) Verfahren zur bekämpfung von pflanzenschädlingen mit nicht-pflanzlichen proteinase-inhibitoren
CA2257198A1 (en) Suppression of specific classes of soybean seed protein genes
DE3584460D1 (de) Verfahren zur erhoehung der ausbeute von heterologen proteinen, produziert durch zuechtung von rekombinanten bakterien.
HUT46068A (en) Gene technological process for producing trypsin inhibitor produced in pancreas by means of recombinant host organism
DE59611434D1 (de) Herstellung von Proteinen aus Pro-Proteinen durch Fusionsproteine abgeleitet von Furin oder Furinanalogen
FI97299C (fi) Menetelmä kimeerisen transformoivan beta-kasvutekijän valmistamiseksi
ATE109156T1 (de) Elastase-inhibierungspolypeptide und verfahren zur herstellung durch genetische rekombination.
DE69739013D1 (de) Verfahren zur Herstellung sekretorischer KEX2-Derivate
DE69430975T2 (de) Verfahren zur Herstellung von Proteinen
ATE253639T1 (de) Verfahren zur herstellung von peptiden
ATE89317T1 (de) Proteine, expressionsvektoren, die fuer solche proteine kodierende dna enthalten und zellen, die solche expressionsvektoren enthalten.
DE69224269T2 (de) Biosynthetisches Verfahren zur Herstellung von Protein
ATE541920T1 (de) Mit stress verbundene proteinphosphatase- polypeptide und verfahren zur verwendung in pflanzen
ATE240400T1 (de) Verfahren zur erzeugung von pathogen-resistenten pflanzen
DE69837047D1 (de) Verfahren zur herstellung verzweigtkettiger fettsäuren mittels genetisch modifizierter pflanzen
NO178504C (no) Fr.måte for fr.stilling av minaktivin,DNA-molekyl,gen,vert,prober,preparat som omfatter prober,reagens for lokalisering og defini. av grenser for tumor i histologiske pröver,samt anvendelse av DNA-molekyl,minaktivin og preparat
DE50109097D1 (de) Promotoren zur genexpression in karyopsen von pflanzen
DE58909205D1 (de) Expressionsvektor und Verfahren zur regulierbaren Herstellung von Proteinen in Eukaryonten.
DE58909308D1 (de) Verfahren zur Herstellung von Tripeptiden.
CA2000661A1 (en) A process for the production of transgenic plants with increased nutritional value via the expression of modified 2s storage albumins

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee