CA2000661A1 - A process for the production of transgenic plants with increased nutritional value via the expression of modified 2s storage albumins - Google Patents

A process for the production of transgenic plants with increased nutritional value via the expression of modified 2s storage albumins

Info

Publication number
CA2000661A1
CA2000661A1 CA2000661A CA2000661A CA2000661A1 CA 2000661 A1 CA2000661 A1 CA 2000661A1 CA 2000661 A CA2000661 A CA 2000661A CA 2000661 A CA2000661 A CA 2000661A CA 2000661 A1 CA2000661 A1 CA 2000661A1
Authority
CA
Canada
Prior art keywords
plants
precursor
nucleic acid
modified
nutritional value
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2000661A
Other languages
French (fr)
Other versions
CA2000661C (en
Inventor
Ann De Clercq
Enno Krebbers
Joel Vandekerckhove
Luiz Barreto De Castro
Eugen Gander
Marc Van Montagu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer CropScience NV
Empresa Brasileira de Pesquisa Agropecuaria EMBRAPA
Original Assignee
Plant Genetic Systems NV
Empresa Brasileira de Pesquisa Agropecuaria EMBRAPA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP88402650A external-priority patent/EP0318341B1/en
Application filed by Plant Genetic Systems NV, Empresa Brasileira de Pesquisa Agropecuaria EMBRAPA filed Critical Plant Genetic Systems NV
Publication of CA2000661A1 publication Critical patent/CA2000661A1/en
Application granted granted Critical
Publication of CA2000661C publication Critical patent/CA2000661C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8253—Methionine or cysteine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention pertains to a process for producing transgenic plants with increased nutritional value. It comprises :
- cultivating plants obtained from regenerated plant cells or from seeds of plants obtained from said regenerated plant cells over one or several generations, whose genetic patrimony, replicable with said plants, comprises a precursor-coding nucleic acid sequence encoding the precursor of a 2S albumin storage protein and placed under the control of a promoter capable of directing gene expression in plants, said precursor-coding nucleic acid being modified in a nonessential region of its relevant sequence which encodes the mature 2S albumin or a subunit thereof with a nucleic acid insert in appropriate reading frame relationship with the surrounding part of said relevant sequence, said insert including a determined segment encoding an heterologous determined polypeptide containing appropriate aminoacid such as lysine and/or methionine and/or threonine and/or phenylalanine and/or tryptophane and/or leucine and/or valine and/or isoleucine.
CA002000661A 1988-10-14 1989-10-13 A process for the production of transgenic plants with increased nutritional value via the expression of modified 2s storage albumins Expired - Fee Related CA2000661C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP88402611 1988-10-14
EP88402650A EP0318341B1 (en) 1987-10-20 1988-10-20 A process for the production of transgenic plants with increased nutritional value via the expression of modified 2S storage albumins in said plants
EP88402611.3 1988-10-20
EP88402650.1 1988-10-20
CA000581160A CA1337048C (en) 1987-10-20 1988-10-25 Process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants

Publications (2)

Publication Number Publication Date
CA2000661A1 true CA2000661A1 (en) 1990-04-14
CA2000661C CA2000661C (en) 1999-04-13

Family

ID=27168086

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002000661A Expired - Fee Related CA2000661C (en) 1988-10-14 1989-10-13 A process for the production of transgenic plants with increased nutritional value via the expression of modified 2s storage albumins

Country Status (4)

Country Link
JP (1) JP2947843B2 (en)
AU (1) AU634987B2 (en)
CA (1) CA2000661C (en)
WO (1) WO1990004032A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI20030315A0 (en) * 2003-02-28 2003-02-28 Joseph Atabekov Methods and constructions for increasing the content of selected amino acids in seeds

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD240911A1 (en) * 1983-12-31 1986-11-19 Adw Der Ddr Zi Fuer Genetik Un METHOD FOR PRODUCING NUTRITIONALLY HYGIENICALLY IMPROVED PLANT SEA PROTEINS
DE3786898T2 (en) * 1986-05-29 1994-02-10 Calgene Inc TRANSFORMATION AND EXPRESSION OF A FOREIGN GENE IN -i (BRASSICA) SPECIES.
AU2811889A (en) * 1987-10-20 1989-05-23 Plant Genetic Systems N.V. A process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants
EP0318341B1 (en) * 1987-10-20 1996-07-31 Plant Genetic Systems, N.V. A process for the production of transgenic plants with increased nutritional value via the expression of modified 2S storage albumins in said plants
AU626821B2 (en) * 1987-10-20 1992-08-13 Plant Genetic Systems N.V. A process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants

Also Published As

Publication number Publication date
JPH03502644A (en) 1991-06-20
CA2000661C (en) 1999-04-13
WO1990004032A1 (en) 1990-04-19
AU634987B2 (en) 1993-03-11
AU4495189A (en) 1990-05-01
JP2947843B2 (en) 1999-09-13

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