DE3840462C2 - - Google Patents
Info
- Publication number
- DE3840462C2 DE3840462C2 DE3840462A DE3840462A DE3840462C2 DE 3840462 C2 DE3840462 C2 DE 3840462C2 DE 3840462 A DE3840462 A DE 3840462A DE 3840462 A DE3840462 A DE 3840462A DE 3840462 C2 DE3840462 C2 DE 3840462C2
- Authority
- DE
- Germany
- Prior art keywords
- microparticles
- measuring
- photomultiplier
- chemiluminescence
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000011859 microparticle Substances 0.000 claims abstract description 28
- 238000000034 method Methods 0.000 claims abstract description 26
- 238000005259 measurement Methods 0.000 claims abstract description 21
- 230000005291 magnetic effect Effects 0.000 claims abstract description 17
- 238000003018 immunoassay Methods 0.000 claims abstract description 7
- 230000003287 optical effect Effects 0.000 claims abstract description 5
- 230000009471 action Effects 0.000 claims abstract description 4
- 239000000126 substance Substances 0.000 claims description 15
- 230000005298 paramagnetic effect Effects 0.000 claims description 13
- 238000004020 luminiscence type Methods 0.000 claims description 11
- 239000003550 marker Substances 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 230000000977 initiatory effect Effects 0.000 claims description 3
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 239000013307 optical fiber Substances 0.000 claims 1
- 230000008569 process Effects 0.000 abstract description 5
- 239000000523 sample Substances 0.000 description 31
- 239000000243 solution Substances 0.000 description 12
- 239000006249 magnetic particle Substances 0.000 description 7
- 239000003298 DNA probe Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 108020003215 DNA Probes Proteins 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- RXNXLAHQOVLMIE-UHFFFAOYSA-N phenyl 10-methylacridin-10-ium-9-carboxylate Chemical compound C12=CC=CC=C2[N+](C)=C2C=CC=CC2=C1C(=O)OC1=CC=CC=C1 RXNXLAHQOVLMIE-UHFFFAOYSA-N 0.000 description 2
- 108020004418 ribosomal RNA Proteins 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 230000032258 transport Effects 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 244000144619 Abrus precatorius Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000009918 complex formation Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000005281 excited state Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000002796 luminescence method Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000006862 quantum yield reaction Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3840462A DE3840462A1 (de) | 1988-12-01 | 1988-12-01 | Verfahren und vorrichtung zur messung der chemilumineszenz |
| DE89119976T DE58906705D1 (de) | 1988-12-01 | 1989-10-27 | Verfahren und Vorrichtung zur Messung der Chemilumineszenz. |
| EP89119976A EP0371265B1 (de) | 1988-12-01 | 1989-10-27 | Verfahren und Vorrichtung zur Messung der Chemilumineszenz |
| AT89119976T ATE100204T1 (de) | 1988-12-01 | 1989-10-27 | Verfahren und vorrichtung zur messung der chemilumineszenz. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3840462A DE3840462A1 (de) | 1988-12-01 | 1988-12-01 | Verfahren und vorrichtung zur messung der chemilumineszenz |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3840462A1 DE3840462A1 (de) | 1990-06-07 |
| DE3840462C2 true DE3840462C2 (enExample) | 1992-10-29 |
Family
ID=6368217
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE3840462A Granted DE3840462A1 (de) | 1988-12-01 | 1988-12-01 | Verfahren und vorrichtung zur messung der chemilumineszenz |
| DE89119976T Expired - Fee Related DE58906705D1 (de) | 1988-12-01 | 1989-10-27 | Verfahren und Vorrichtung zur Messung der Chemilumineszenz. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE89119976T Expired - Fee Related DE58906705D1 (de) | 1988-12-01 | 1989-10-27 | Verfahren und Vorrichtung zur Messung der Chemilumineszenz. |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP0371265B1 (enExample) |
| AT (1) | ATE100204T1 (enExample) |
| DE (2) | DE3840462A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19520298A1 (de) * | 1995-06-02 | 1996-12-05 | Bayer Ag | Sortiervorrichtung für biologische Zellen oder Viren |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5128103A (en) * | 1990-12-14 | 1992-07-07 | E. I. Du Pont De Nemours And Company | Apparatus for automatically processing magnetic solid phase reagents |
| DE4041080A1 (de) | 1990-12-21 | 1992-06-25 | Behringwerke Ag | Verfahren zur bestimmung eines analyten |
| US6436349B1 (en) | 1991-03-04 | 2002-08-20 | Bayer Corporation | Fluid handling apparatus for an automated analyzer |
| CA2384529C (en) * | 1991-03-04 | 2007-01-02 | Bayer Corporation | Automated analyzer |
| DE4429155A1 (de) * | 1994-08-17 | 1996-02-22 | Hans Schiesl | Meßanordnung und Verfahren zur Durchführung luminometrischer Reihenanalysen sowie Mehrfachküvette zur Aufnahme von Flüssigkeitsproben hierfür |
| US5599501A (en) * | 1994-11-10 | 1997-02-04 | Ciba Corning Diagnostics Corp. | Incubation chamber |
| US5888835A (en) * | 1996-05-10 | 1999-03-30 | Chiron Diagnostics Corporation | Method and apparatus for wash, resuspension, recollection and localization of magnetizable particles in assays using magnetic separation technology |
| US6143578A (en) * | 1996-05-10 | 2000-11-07 | Bayer Corporation | Method and apparatus for wash, resuspension, recollection and localization of magnetizable particles in assays using magnetic separation technology |
| US5856194A (en) | 1996-09-19 | 1999-01-05 | Abbott Laboratories | Method for determination of item of interest in a sample |
| US5795784A (en) | 1996-09-19 | 1998-08-18 | Abbott Laboratories | Method of performing a process for determining an item of interest in a sample |
| EP3116650B1 (de) | 2014-03-10 | 2020-03-04 | PAIA Biotech GmbH | Verfahren und vorrichtung zur bestimmung biologischer analyten |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1782445A1 (enExample) * | 1962-09-12 | |||
| EP0030086B2 (en) * | 1979-11-13 | 1990-03-14 | TECHNICON INSTRUMENTS CORPORATION (a New York corporation) | Test-tube assembly, kit for making it and method of manual immunoassay |
| GB8401368D0 (en) * | 1984-01-19 | 1984-02-22 | Amersham Int Plc | Assay method |
| FI850481A0 (fi) * | 1985-02-06 | 1985-02-06 | Labsystems Oy | Foerfarande foer bestaemning av motmedel eller antigener. |
| JPH0737989B2 (ja) * | 1986-07-04 | 1995-04-26 | 東ソー株式会社 | 免疫反応の測定方法および装置 |
| DE3623601C2 (de) * | 1986-07-12 | 1997-01-16 | Berthold Lab Prof Dr | Meßgerät für Bio- und Chemilumineszenz |
| GB8620893D0 (en) * | 1986-08-29 | 1986-10-08 | Unilever Plc | Assays |
| ZA877772B (en) * | 1986-10-23 | 1988-04-20 | Amoco Corporation | Target and background capture methods and apparatus for affinity assays |
-
1988
- 1988-12-01 DE DE3840462A patent/DE3840462A1/de active Granted
-
1989
- 1989-10-27 AT AT89119976T patent/ATE100204T1/de not_active IP Right Cessation
- 1989-10-27 EP EP89119976A patent/EP0371265B1/de not_active Expired - Lifetime
- 1989-10-27 DE DE89119976T patent/DE58906705D1/de not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19520298A1 (de) * | 1995-06-02 | 1996-12-05 | Bayer Ag | Sortiervorrichtung für biologische Zellen oder Viren |
Also Published As
| Publication number | Publication date |
|---|---|
| DE58906705D1 (de) | 1994-02-24 |
| EP0371265A1 (de) | 1990-06-06 |
| ATE100204T1 (de) | 1994-01-15 |
| EP0371265B1 (de) | 1994-01-12 |
| DE3840462A1 (de) | 1990-06-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| 8127 | New person/name/address of the applicant |
Owner name: LABORATORIUM PROF. DR. RUDOLF BERTHOLD GMBH & CO K |
|
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: BERTHOLD TECHNOLOGIES GMBH & CO. KG, 75323 BAD WIL |
|
| 8339 | Ceased/non-payment of the annual fee |