DE3751326D1 - Durch rekombinante DNS-Technologie konstruierte amylolytische enzymproduzierende Mikroorganismen und ihre Verwendung in Gärungsverfahren. - Google Patents

Durch rekombinante DNS-Technologie konstruierte amylolytische enzymproduzierende Mikroorganismen und ihre Verwendung in Gärungsverfahren.

Info

Publication number
DE3751326D1
DE3751326D1 DE3751326T DE3751326T DE3751326D1 DE 3751326 D1 DE3751326 D1 DE 3751326D1 DE 3751326 T DE3751326 T DE 3751326T DE 3751326 T DE3751326 T DE 3751326T DE 3751326 D1 DE3751326 D1 DE 3751326D1
Authority
DE
Germany
Prior art keywords
amylolytic enzymes
recombinant dna
fermentation processes
dna technology
producing microorganisms
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE3751326T
Other languages
English (en)
Other versions
DE3751326T2 (de
Inventor
Alexander Strasser
Feodor Bernard Martens
Juergen Dohmen
Cornelius P Hollenberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Heineken Technical Services BV
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of DE3751326D1 publication Critical patent/DE3751326D1/de
Publication of DE3751326T2 publication Critical patent/DE3751326T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/047—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C12/00—Processes specially adapted for making special kinds of beer
    • C12C12/002—Processes specially adapted for making special kinds of beer using special microorganisms
    • C12C12/004—Genetically modified microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C12/00—Processes specially adapted for making special kinds of beer
    • C12C12/002—Processes specially adapted for making special kinds of beer using special microorganisms
    • C12C12/006—Yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C12/00—Processes specially adapted for making special kinds of beer
    • C12C12/02—Beer with low calorie content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE3751326T 1986-08-21 1987-07-17 Durch rekombinante DNS-Technologie konstruierte amylolytische enzymproduzierende Mikroorganismen und ihre Verwendung in Gärungsverfahren. Expired - Fee Related DE3751326T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP86111586A EP0257115A1 (de) 1986-08-21 1986-08-21 Durch rekombinante DNS-Technologie konstruierte amylolytische enzymproduzierende Mikroorganismen und ihre Verwendung in Gärungsverfahren

Publications (2)

Publication Number Publication Date
DE3751326D1 true DE3751326D1 (de) 1995-07-06
DE3751326T2 DE3751326T2 (de) 1996-01-11

Family

ID=8195356

Family Applications (2)

Application Number Title Priority Date Filing Date
DE3751326T Expired - Fee Related DE3751326T2 (de) 1986-08-21 1987-07-17 Durch rekombinante DNS-Technologie konstruierte amylolytische enzymproduzierende Mikroorganismen und ihre Verwendung in Gärungsverfahren.
DE198787110370T Pending DE260404T1 (de) 1986-08-21 1987-07-17 Durch rekombinante dns-technologie konstruierte amylolytische enzymproduzierende mikroorganismen und ihre verwendung in gaerungsverfahren.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE198787110370T Pending DE260404T1 (de) 1986-08-21 1987-07-17 Durch rekombinante dns-technologie konstruierte amylolytische enzymproduzierende mikroorganismen und ihre verwendung in gaerungsverfahren.

Country Status (7)

Country Link
US (1) US5100794A (de)
EP (1) EP0257115A1 (de)
AT (1) ATE123304T1 (de)
DE (2) DE3751326T2 (de)
ES (1) ES2003314T3 (de)
GR (1) GR880300088T1 (de)
ZA (1) ZA876134B (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2613726B2 (fr) * 1986-06-10 1990-07-20 Transgene Sa Integration dans la souche polyploide de boulangerie (tgf1) du bloc d'expression de l'alpha-amyloglucosidase de aspergillus niger
FR2613727B2 (fr) * 1986-06-10 1990-07-20 Transgene Sa Integration dans la souche polyploide saccharomyces uvarum 2285 tg2 amgc du bloc d'expression de l'alpha-amylase de b. licheniformis
WO1989009269A1 (en) * 1988-03-31 1989-10-05 The Upjohn Company The gene encoding phosphoribosylaminoimidazolecarboxylase of schwanniomyces occidentalis
ES2038579T3 (es) * 1989-04-28 1997-02-16 Rhein Biotech Proz & Prod Gmbh Celulas de levadura del genero schwanniomyces.
US5665585A (en) * 1992-09-03 1997-09-09 Alko-Yhiot Oy Recombinant production of glucoamylase P in trichoderma
AU6415594A (en) * 1993-03-25 1994-10-11 Biosource Genetics Corporation (pichia pastoris) alcohol oxidase (zza1) and (zza2)
US6551798B2 (en) * 2000-08-04 2003-04-22 Battelle Memorial Institute Method for using a yeast alpha-amylase promoter
US6541622B1 (en) 2000-08-04 2003-04-01 Battelle Memorial Institute Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression
US6900305B2 (en) 2000-08-04 2005-05-31 Battelle Memorial Institute Isolated yeast promoter sequence and a method of regulated heterologous expression
CN102112596B (zh) * 2008-05-06 2014-06-18 阿彻丹尼尔斯米德兰德公司 通过淀粉和木聚糖的降解酶的表达开发能进行淀粉和木聚糖的醇发酵的耐热酵母多形汉逊酵母菌株
US20100291649A1 (en) * 2009-05-18 2010-11-18 E. I. Du Pont De Nemours And Company Control of contaminant yeast in fermentation processes
BR112013025753A8 (pt) 2011-04-05 2018-06-12 Lallemand Hungary Liquidity Man Llc Métodos para aprimoramento do rendimento de produto e produção em um micro-organismo através da adição de aceptores de elétrons alternativos
GB201620658D0 (en) * 2016-12-05 2017-01-18 Univ Stellenbosch Recombinant yeast and use thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE55817B1 (en) * 1982-05-19 1991-01-30 Gist Brocades Nv Cloning system for kluyveromyces species
NO840200L (no) * 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
CA1203765A (en) * 1983-05-11 1986-04-29 Alexander M. Sills Schwanniomyces castellii strains and brewing process
US4794175A (en) * 1983-12-20 1988-12-27 Cetus Corporation Glucoamylase CDNA
IL75210A0 (en) * 1984-05-22 1985-09-29 Bioteknika International Yeast vector
IL76190A0 (en) * 1984-08-31 1985-12-31 Smithkline Beckman Corp Recombinant saccharomyces
JPS61141890A (ja) * 1984-12-15 1986-06-28 Suntory Ltd アルコ−ルの製造法
GB8432483D0 (en) * 1984-12-21 1985-02-06 Tubb R S Enzymes
WO1986007091A1 (en) * 1985-05-21 1986-12-04 Biotechnica International, Inc. Yeast expressing glucoamylase

Also Published As

Publication number Publication date
EP0257115A1 (de) 1988-03-02
GR880300088T1 (en) 1988-10-21
ZA876134B (en) 1988-02-29
ES2003314T3 (es) 1996-10-01
US5100794A (en) 1992-03-31
ATE123304T1 (de) 1995-06-15
DE3751326T2 (de) 1996-01-11
DE260404T1 (de) 1988-09-01
ES2003314A4 (es) 1988-11-01

Similar Documents

Publication Publication Date Title
Yokoi et al. Microbial hydrogen production from sweet potato starch residue
DE3751326D1 (de) Durch rekombinante DNS-Technologie konstruierte amylolytische enzymproduzierende Mikroorganismen und ihre Verwendung in Gärungsverfahren.
KR850001528A (ko) 열안정성 글루코아밀라제의 제법
ATE43634T1 (de) Maltose erzeugendes amylase-enzymprodukt, dessen herstellung und dessen verwendung.
IL115834A0 (en) Process for bacterial production of polypeptides
GB2133408A (en) Method for improving the production of proteins in bacillus
DE3588008D1 (de) Hefestämme zur erzeugung cellulolytischer enzyme und verfahren und mittel zu deren herstellung.
ATE69266T1 (de) Verfahren zum herstellung von insulin-vorlaeufern und verfahren zur herstellung von humaninsulin.
AU2443488A (en) Dna-molecules coding for fmdh control regions and structured gene for a protein having fmdh-activity and their uses
ATE46358T1 (de) Verfahren zur zuechtung von lyophilisierten mikroorganismen.
IL148762A (en) Preparation of 1,3- propanediol by fermentaiton of glycerol
ATE429492T1 (de) Dna-kette zur erhöhung der produktion von karotenoiden
AU4361889A (en) Biocatalysts
KR850001279A (ko) α-아밀라제의 제법
AU621810B2 (en) Amylolytic enzymes producing microorganisms, constructed by recombinant DNA technology and their use for fermentation processes
ES2061536T3 (es) Procedimiento para la obtencion de glucosa dehidrogenasa a partir de bacillus megaterium.
ATE97954T1 (de) Beta-amylase-enzym-produkt, herstellung und verwendung.
ES8504245A1 (es) Procedimiento para la obtencion de sarcosin-oxidasa
ATE46363T1 (de) Klonierungs- und expressionsplasmidvektoren fuer ein protein in einem mikroorganismus die mindestens den expressionspromotor von betaglucosidase in den hefen enthalten, dieselben enthaltende mikroorganismen, gaerungsverfahren und erhaltene enzyme.
ATE107959T1 (de) Klonierung des isoamylaseenzym kodierenden gens und seine verwendung zur herstellung dieses enzyms.
EP0289908A3 (de) Microorganismen und Verfahren zum Abbauen von pflanzlichen Zellwänden und von komplexen Kohlenwasserstoffen
ES2052760T3 (es) Nuevo sistema enzimatico, su procedimiento de preparacion, y su aplicacion particularmente a la preparacion de d-parahidroxifenilglicina.
Fiedurek et al. Seeds as natural matrices for immobilization of Aspergillus niger mycelium producing pectinases
EP0258038B1 (de) Verwendung von Zusammensetzungen auf Basis von Cellulase für die Kultivierung und Anwendung von Mikroorganismen, welche Zellulose herstellen
DE3161317D1 (en) Process for the production of glucose dehydrogenase and microorganisms suitable therefor

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: HEINEKEN TECHNICAL SERVICES B.V., ZOETERWOUDE, NL

8339 Ceased/non-payment of the annual fee