DE3689398D1 - Verfahren zum Extrahieren von Nukleinsäuren aus Zellen. - Google Patents

Verfahren zum Extrahieren von Nukleinsäuren aus Zellen.

Info

Publication number
DE3689398D1
DE3689398D1 DE86300075T DE3689398T DE3689398D1 DE 3689398 D1 DE3689398 D1 DE 3689398D1 DE 86300075 T DE86300075 T DE 86300075T DE 3689398 T DE3689398 T DE 3689398T DE 3689398 D1 DE3689398 D1 DE 3689398D1
Authority
DE
Germany
Prior art keywords
cells
nucleic acids
extracting nucleic
extracting
acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE86300075T
Other languages
English (en)
Other versions
DE3689398T2 (de
Inventor
Guy Richard Cathcart
Paul David Grossman
Norman Whiteley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Applied Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Biosystems Inc filed Critical Applied Biosystems Inc
Publication of DE3689398D1 publication Critical patent/DE3689398D1/de
Application granted granted Critical
Publication of DE3689398T2 publication Critical patent/DE3689398T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Extraction Or Liquid Replacement (AREA)
DE19863689398 1985-01-18 1986-01-07 Verfahren zum Extrahieren von Nukleinsäuren aus Zellen. Expired - Lifetime DE3689398T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US69295885A 1985-01-18 1985-01-18

Publications (2)

Publication Number Publication Date
DE3689398D1 true DE3689398D1 (de) 1994-01-27
DE3689398T2 DE3689398T2 (de) 1994-07-14

Family

ID=24782760

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19863689398 Expired - Lifetime DE3689398T2 (de) 1985-01-18 1986-01-07 Verfahren zum Extrahieren von Nukleinsäuren aus Zellen.

Country Status (4)

Country Link
EP (1) EP0215533B1 (de)
JP (1) JPH0815437B2 (de)
CA (1) CA1335963C (de)
DE (1) DE3689398T2 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0240191A3 (de) * 1986-03-13 1989-10-25 Seiko Instruments Inc. Verfahren zur Isolierung von in einem Virus oder einer Zelle enthaltender DNS
EP0245945B1 (de) * 1986-04-11 1993-11-10 Applied Biosystems, Inc. Automatisches Gerät zum Extrahieren von Nukleinsäuren
SE8801098D0 (sv) * 1988-03-24 1988-03-24 Pharmacia Ab Apparatus and method for automatic extraction of extra-chromosomal dna from a cell suspension in a container
JPH04505848A (ja) * 1989-06-02 1992-10-15 ユーレン、マチアス 細胞懸濁液から染色体外ｄｎａを自動精製する装置と方法
US5128247A (en) * 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
KR20020029477A (ko) * 2000-10-13 2002-04-19 박제철 한 단계에 의한 유전자 추출방법
KR20020029476A (ko) * 2000-10-13 2002-04-19 박제철 한 단계에 의해 유전자를 추출하기 위한 라이시스버퍼시약
DE102007025276A1 (de) * 2007-05-31 2008-12-04 Qiagen Gmbh Aromatische Alkohole zur Behandlung einer biologischen Probe
CA2682439A1 (en) * 2008-10-17 2010-04-17 F. Hoffmann-La Roche Ag Cell monitoring and molecular analysis
EP3210667A1 (de) * 2016-02-29 2017-08-30 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Modulare vorrichtung für kontinuierlichen fluss
CN113801876A (zh) 2017-01-16 2021-12-17 光谱解决方案有限责任公司 核酸保存溶液及其制造和使用方法
AU2019384801A1 (en) 2018-11-20 2021-06-10 Spectrum Solutions, Llc Sample collection system including sealing cap and valve
US11701094B2 (en) 2019-06-20 2023-07-18 Spectrum Solutions L.L.C. Sample collection system including valve and plug assemblies

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL136773C (de) * 1960-07-04
FR93454E (fr) * 1963-03-29 1969-04-04 Saint Gobain Tech Nouvelles Et Appareil pour la mise en contact de deux liquides normalement non miscibles.
US3389133A (en) * 1966-08-10 1968-06-18 Schwarz Biores Inc Process for isolating and purifying soluble ribonucleic acid
US3582468A (en) * 1968-09-27 1971-06-01 Merck & Co Inc Recovery of double-stranded ribonucleic acid
EP0008189A1 (de) * 1978-08-04 1980-02-20 DAVY MCKEE (MINERALS & METALS) LIMITED Vorrichtung zur Flüssig-Flüssig-Extraktion
US4338285A (en) * 1978-12-06 1982-07-06 A. H. Ross & Associates Liquid-liquid contact apparatus
JPS5818076B2 (ja) * 1979-12-28 1983-04-11 上田 誠之助 リボ核酸の製造法
GB2143238A (en) * 1983-03-31 1985-02-06 Dr Anthony Stuart Breeze A method for enzyme liberation from bacterial cells
DD230560A3 (de) * 1983-11-07 1985-12-04 Leiser Robert Matthias Verfahren zur isolierung und partiellen reinigung von rna
GB8331071D0 (en) * 1983-11-22 1983-12-29 Karayiannis P Assay for dna/rna

Also Published As

Publication number Publication date
EP0215533A2 (de) 1987-03-25
JPS61167697A (ja) 1986-07-29
EP0215533A3 (en) 1988-06-22
EP0215533B1 (de) 1993-12-15
JPH0815437B2 (ja) 1996-02-21
CA1335963C (en) 1995-06-20
DE3689398T2 (de) 1994-07-14

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: THE PERKIN-ELMER CORP. (N.D.GES.D.STAATES NEW YORK

8327 Change in the person/name/address of the patent owner

Owner name: APPLERA CORP., FOSTER CITY, CALIF., US