DE3650720T2 - Einschrittverfahren und Polynukleotidverbindungen zur Hybridisierung mit Ziel-Polynukleotiden - Google Patents

Einschrittverfahren und Polynukleotidverbindungen zur Hybridisierung mit Ziel-Polynukleotiden

Info

Publication number
DE3650720T2
DE3650720T2 DE3650720T DE3650720T DE3650720T2 DE 3650720 T2 DE3650720 T2 DE 3650720T2 DE 3650720 T DE3650720 T DE 3650720T DE 3650720 T DE3650720 T DE 3650720T DE 3650720 T2 DE3650720 T2 DE 3650720T2
Authority
DE
Germany
Prior art keywords
polynucleotide
hybridization
connections
step method
target polynucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Revoked
Application number
DE3650720T
Other languages
English (en)
Other versions
DE3650720D1 (de
Inventor
Jannis Stavrianopoulos
Elazar Rabbani
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enzo Biochem Inc
Original Assignee
Enzo Biochem Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25199634&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3650720(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Enzo Biochem Inc filed Critical Enzo Biochem Inc
Publication of DE3650720D1 publication Critical patent/DE3650720D1/de
Application granted granted Critical
Publication of DE3650720T2 publication Critical patent/DE3650720T2/de
Anticipated expiration legal-status Critical
Revoked legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE3650720T 1985-12-13 1986-12-15 Einschrittverfahren und Polynukleotidverbindungen zur Hybridisierung mit Ziel-Polynukleotiden Revoked DE3650720T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US80875785A 1985-12-13 1985-12-13

Publications (2)

Publication Number Publication Date
DE3650720D1 DE3650720D1 (de) 1999-07-22
DE3650720T2 true DE3650720T2 (de) 2000-03-16

Family

ID=25199634

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3650720T Revoked DE3650720T2 (de) 1985-12-13 1986-12-15 Einschrittverfahren und Polynukleotidverbindungen zur Hybridisierung mit Ziel-Polynukleotiden

Country Status (6)

Country Link
US (1) US8334370B1 (de)
EP (2) EP0231495B1 (de)
JP (3) JP3293820B2 (de)
AT (1) ATE181366T1 (de)
CA (1) CA1339096C (de)
DE (1) DE3650720T2 (de)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4948882A (en) * 1983-02-22 1990-08-14 Syngene, Inc. Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis
DE3639109A1 (de) * 1986-11-15 1988-05-19 Merck Patent Gmbh Verfahren zur bestimmung von nucleinsaeuren
US5185439A (en) * 1987-10-05 1993-02-09 Gen-Probe Incorporated Acridinium ester labelling and purification of nucleotide probes
PT88665B (pt) * 1987-10-05 1992-12-31 Ml Tecnology Ventures Lp Metodo para a marcacao com ester de acridinio e purificacao de sondas nucleotidicas
US5099011A (en) * 1988-08-02 1992-03-24 Ortho Diagnostic Systems Inc. Nucleic acid probe for detection of Neisseria gonorrhoea
US5047523A (en) * 1988-08-02 1991-09-10 Ortho Diagnostic Systems Inc. Nucleic acid probe for detection of neisseria gonorrhoea
US5324829A (en) * 1988-12-16 1994-06-28 Ortho Diagnostic Systems, Inc. High specific activity nucleic acid probes having target recognition and signal generating moieties
CA2005927A1 (en) * 1988-12-21 1990-06-21 Chander Bahl Method of preparing nucleotide probes using a bridging complement
DE69003104T2 (de) * 1989-06-12 1994-03-17 Cis Bio International Saclay Verfahren zum nachweis von spezifischen sequenzen von nukleinsäuren und ihre verwendungen.
FR2650839A1 (fr) * 1989-08-08 1991-02-15 Oris Ind Cie Procede de detection en phase homogene liquide de sequences specifiques d'acides nucleiques et ses applications
ATE193019T1 (de) * 1989-10-13 2000-06-15 Zeneca Ltd Sonden
GB8927365D0 (en) * 1989-12-04 1990-01-31 Ici Plc Reagent
DE69132765T2 (de) * 1990-12-24 2002-02-21 Enzo Diagnostics Inc Verfahren zum Nachweis eines Zielpolynukleotids in einer Probe unter Verwendung eines Reagenz, das den Hintergrund verringert, und eine dieses Reagenz enthaltende Zusammensetzung und Kit.
CA2110946A1 (en) * 1992-12-09 1994-06-10 Elazar Rabbani Induction of immunocompatibility by nucleic acid
CA2140081C (en) 1994-01-13 2008-04-01 Dean L. Engelhardt Process, construct and conjugate for producing multiple nucleic acid copies
US5616465A (en) 1995-08-09 1997-04-01 The Regents Of The University Of California Detection and isolation of nucleic acid sequences using competitive hybridization probes
US6027879A (en) * 1995-08-09 2000-02-22 The Regents Of The University Of California Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe
WO1998005766A1 (fr) * 1996-08-02 1998-02-12 Bio Merieux Procede d'amplification d'une sequence d'un acide nucleique cible
SE522077C2 (sv) * 1997-09-05 2004-01-13 Lightup Technologies Ab Förfarande att välja sekvens hos sond för nukleinsyrahybridisering
US8445664B2 (en) 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6472153B1 (en) 1999-10-26 2002-10-29 Epoch Biosciences, Inc. Hybridization-triggered fluorescent detection of nucleic acids
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US9777312B2 (en) 2001-06-30 2017-10-03 Enzo Life Sciences, Inc. Dual polarity analysis of nucleic acids
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
AU2002358462A1 (en) * 2001-12-18 2003-06-30 Human Genetic Signatures Pty Ltd Linear and hairpin oligonucleotide analogues comprising intercalator pseudonucleotides
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
BRPI0607213B1 (pt) 2005-01-28 2017-04-04 Univ Duke aparelho para manipulação de gotículas em uma placa de circuito impresso
US8357801B2 (en) 2005-05-24 2013-01-22 Enzo Life Sciences, Inc. Labeling of target molecules, identification of organelles and other applications, novel compositions, methods and kits
US7737281B2 (en) 2005-05-24 2010-06-15 Enzo Life Sciences, Inc. C/O Enzo Biochem, Inc. Purine based fluorescent dyes
US7569695B2 (en) 2005-05-24 2009-08-04 Enzo Life Sciences, Inc. Dyes for the detection or quantification of desirable target molecules

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
CA1190838A (en) * 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
CA1223831A (en) * 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
US4547569A (en) * 1982-11-24 1985-10-15 The United States Of America As Represented By The Department Of Health And Human Services Intercalating agents specifying nucleotides
FR2540122B1 (fr) * 1983-01-27 1985-11-29 Centre Nat Rech Scient Nouveaux composes comportant une sequence d'oligonucleotide liee a un agent d'intercalation, leur procede de synthese et leur application
AU596068B2 (en) 1983-02-22 1990-04-26 Syngene, Inc. Defined sequence single strand oligonucleotides incorporating reporter groups, process for the chemical synthesis thereof, and nucleosides useful in such synthesis
ES532041A0 (es) * 1983-05-02 1985-10-01 Enzo Biochem Inc Un metodo para detectar una secuencia seleccionada de acido nucleico en un material biologico.
CA1222705A (en) * 1983-07-14 1987-06-09 Molecular Diagnostics, Inc. Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays
DK487784A (da) * 1983-10-13 1985-04-14 Univ Georgia Immunoassay
EP0144914A3 (de) * 1983-12-12 1986-08-13 Miles Inc. Hybridisierungstest mittels markierter Paare von hybridbindenden Reagenzien
US4707352A (en) * 1984-01-30 1987-11-17 Enzo Biochem, Inc. Method of radioactively labeling diagnostic and therapeutic agents containing a chelating group
CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
US4820630A (en) * 1984-11-23 1989-04-11 Digene Diagnostics, Incorporated Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels
CA1295559C (en) 1985-08-13 1992-02-11 Jannis Stavrianopoulos Method for labeling polynucleotide sequences
CA1273552A (en) * 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
DE69132765T2 (de) * 1990-12-24 2002-02-21 Enzo Diagnostics Inc Verfahren zum Nachweis eines Zielpolynukleotids in einer Probe unter Verwendung eines Reagenz, das den Hintergrund verringert, und eine dieses Reagenz enthaltende Zusammensetzung und Kit.
US5547861A (en) 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification

Also Published As

Publication number Publication date
DE3650720D1 (de) 1999-07-22
JPH07322900A (ja) 1995-12-12
JP2002356496A (ja) 2002-12-13
US8334370B1 (en) 2012-12-18
JP3293820B2 (ja) 2002-06-17
EP0231495B1 (de) 1999-06-16
EP0916737A3 (de) 2002-03-06
ATE181366T1 (de) 1999-07-15
EP0916737A2 (de) 1999-05-19
JPS62163699A (ja) 1987-07-20
JP3814669B2 (ja) 2006-08-30
EP0231495A3 (de) 1990-07-18
CA1339096C (en) 1997-07-29
JP2735813B2 (ja) 1998-04-02
EP0231495A2 (de) 1987-08-12

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Legal Events

Date Code Title Description
8363 Opposition against the patent
8331 Complete revocation