DE3507407A1 - Durchfluss-zytometriegeraet - Google Patents

Durchfluss-zytometriegeraet

Info

Publication number
DE3507407A1
DE3507407A1 DE19853507407 DE3507407A DE3507407A1 DE 3507407 A1 DE3507407 A1 DE 3507407A1 DE 19853507407 DE19853507407 DE 19853507407 DE 3507407 A DE3507407 A DE 3507407A DE 3507407 A1 DE3507407 A1 DE 3507407A1
Authority
DE
Germany
Prior art keywords
light
light beam
cells
flow cytometry
prism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
DE19853507407
Other languages
German (de)
English (en)
Other versions
DE3507407C2 (enExample
Inventor
Vernon L. Los Altos Calif. Chupp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of DE3507407A1 publication Critical patent/DE3507407A1/de
Application granted granted Critical
Publication of DE3507407C2 publication Critical patent/DE3507407C2/de
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1497—Particle shape

Landscapes

  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
  • Optical Measuring Cells (AREA)
DE19853507407 1984-03-05 1985-03-02 Durchfluss-zytometriegeraet Granted DE3507407A1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/586,162 US4545677A (en) 1984-03-05 1984-03-05 Prismatic beam expander for light beam shaping in a flow cytometry apparatus

Publications (2)

Publication Number Publication Date
DE3507407A1 true DE3507407A1 (de) 1985-09-12
DE3507407C2 DE3507407C2 (enExample) 1988-11-24

Family

ID=24344558

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19853507407 Granted DE3507407A1 (de) 1984-03-05 1985-03-02 Durchfluss-zytometriegeraet

Country Status (3)

Country Link
US (1) US4545677A (enExample)
JP (1) JPS60195436A (enExample)
DE (1) DE3507407A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987005108A1 (en) * 1986-02-12 1987-08-27 Combustion Engineering, Inc. An in situ particle size measuring device cross-reference to related applications

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62102705U (enExample) * 1985-12-19 1987-06-30
JPS62293143A (ja) * 1986-06-12 1987-12-19 Rion Co Ltd 微粒子測定装置
JPS63214668A (ja) * 1987-03-03 1988-09-07 Hitachi Ltd 細胞測定方法
JPH07104349B2 (ja) * 1987-04-11 1995-11-13 株式会社日立製作所 細胞測定法
JPH01196536A (ja) * 1988-02-01 1989-08-08 Canon Inc 粒子解析装置
JPH02289808A (ja) * 1989-04-28 1990-11-29 Olympus Optical Co Ltd 照明光学系
US5132548A (en) * 1990-09-14 1992-07-21 High Yield Technology High sensitivity, large detection area particle sensor for vacuum applications
US5489977A (en) * 1993-08-11 1996-02-06 Texaco Inc. Photomeric means for monitoring solids and fluorescent material in waste water using a falling stream water sampler
JP4323571B2 (ja) 1997-01-31 2009-09-02 エックスワイ， インコーポレイテッド 光学装置
US7576909B2 (en) 1998-07-16 2009-08-18 Imra America, Inc. Multimode amplifier for amplifying single mode light
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US6071689A (en) 1997-12-31 2000-06-06 Xy, Inc. System for improving yield of sexed embryos in mammals
US6154276A (en) * 1998-02-23 2000-11-28 The Regents Of The University Of California Waveguide detection of right-angle-scattered light in flow cytometry
US6542304B2 (en) 1999-05-17 2003-04-01 Toolz, Ltd. Laser beam device with apertured reflective element
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
CA2408939C (en) 2000-05-09 2011-11-08 Xy, Inc. High purity x-chromosome bearing and y-chromosome bearing populations of spermatozoa
WO2002043574A2 (en) 2000-11-29 2002-06-06 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US8211629B2 (en) 2002-08-01 2012-07-03 Xy, Llc Low pressure sperm cell separation system
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
CA2534394C (en) 2002-08-15 2013-01-08 Xy, Inc. High resolution flow cytometer
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
US20040061853A1 (en) * 2002-09-27 2004-04-01 Blasenheim Barry J. Prism-based flow cytometry excitation optics
JP3891925B2 (ja) * 2002-12-03 2007-03-14 ベイバイオサイエンス株式会社 生物学的粒子の情報を得る装置
DK2308419T3 (da) 2003-03-28 2016-06-06 Inguran Llc Fremgangsmåde til bedømmelse af farvningstilstandene for dyresperm, som skal sorteres.
EP1625203B1 (en) 2003-05-15 2015-04-08 Xy, Llc Efficient haploid cell sorting for flow cytometer systems
WO2005095590A2 (en) 2004-03-29 2005-10-13 Monsanto Technology Llc Sperm suspensions for sorting into x or y chromosome-bearing enriched populations
JP4304120B2 (ja) * 2004-04-30 2009-07-29 ベイバイオサイエンス株式会社 生物学的粒子をソーティングする装置及び方法
AR049732A1 (es) 2004-07-22 2006-08-30 Monsanto Co Proceso para enriquecer una poblacion de celulas de esperma
US7110192B2 (en) * 2005-01-12 2006-09-19 Dako Denmark A/S System and method for a composite lens for a flow cytometer
US20070095667A1 (en) * 2005-10-27 2007-05-03 Applera Corporation Optoelectronic Separation of Biomolecules
WO2009063322A2 (en) * 2007-08-15 2009-05-22 Malvern Instruments Ltd Broad-range spectrometer
JP5571845B2 (ja) * 2010-05-03 2014-08-13 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド フローサイトメーターにおいて複数のレーザービームを合波するための光学コンバイナ
CN103091211B (zh) * 2011-11-03 2017-05-17 深圳迈瑞生物医疗电子股份有限公司 荧光检测系统和细胞分析仪
JP6338597B2 (ja) 2013-01-09 2018-06-06 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアＴｈｅ Ｒｅｇｅｎｔｓ Ｏｆ Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃａｌｉｆｏｒｎｉａ 無線周波数多重励起を用いた蛍光イメージングのための装置及び方法
ES2959506T3 (es) 2014-03-18 2024-02-26 Univ California Citómetro de flujo paralelo que usa multiplexación por radiofrecuencia, y método
JP6856635B2 (ja) 2015-10-13 2021-04-07 オメガ バイオシステムズ インコーポレイテッド マルチモードの蛍光撮像フローサイトメトリシステム
CN109374511B (zh) * 2015-10-14 2021-07-23 北京信息科技大学 一种流式细胞仪无液路情况的光路调整装置
AU2017234815B2 (en) * 2016-03-17 2022-11-03 Becton, Dickinson And Company Cell sorting using a high throughput fluorescence flow cytometer
KR102381176B1 (ko) 2016-05-12 2022-04-01 비디 바이오사이언시스 개선된 이미지 해상도를 갖는 형광 이미징 유세포 분석법
US10006852B2 (en) 2016-09-13 2018-06-26 Becton, Dickinson And Company Flow cytometer with optical equalization
CN106918544A (zh) * 2017-03-29 2017-07-04 中国科学院苏州生物医学工程技术研究所 一种基于荧光激发光源的流式分析仪系统
CN110945343B (zh) 2017-07-19 2023-01-13 英格朗公司 分析仪器和产生精子群体的方法
US11648551B2 (en) 2017-12-12 2023-05-16 Essenlix Corporation Sample manipulation and assay with rapid temperature change
EP3997439A4 (en) 2019-07-10 2023-07-19 Becton, Dickinson and Company RECONFIGURABLE INTEGRATED CIRCUITS TO ADJUST CELL SORTING CLASSIFICATION
US11513058B2 (en) 2020-05-19 2022-11-29 Becton, Dickinson And Company Methods for modulating an intensity profile of a laser beam and systems for same
EP4610627A1 (en) 2020-06-26 2025-09-03 Becton Dickinson and Company Dual excitation beams for irradiating a sample in a flow stream and methods for using same
CN112433384A (zh) * 2020-12-16 2021-03-02 桂林优利特医疗电子有限公司 一种用于流式细胞仪的多色激光光路系统

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2246380A1 (de) * 1972-05-22 1973-12-20 Univ Leland Stanford Junior Verfahren und vorrichtung zum sortieren von teilchen
DE2732272B1 (de) * 1977-07-16 1978-10-26 Deutsches Krebsforsch Verfahren und Vorrichtung zur Fluoreszenzanalyse von gefaerbten Partikeln,insbesondere biologischen Zellen
DE3310665A1 (de) * 1982-03-25 1983-10-06 Becton Dickinson Co Vorrichtung zur messung von lichtoptischen parametern von partikeln

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4084881A (en) * 1975-10-21 1978-04-18 Canon Kabushiki Kaisha Light beam scanning device
JPS5326996A (en) * 1976-08-26 1978-03-13 Tokyo Keiki Kk Compound dielectric body

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2246380A1 (de) * 1972-05-22 1973-12-20 Univ Leland Stanford Junior Verfahren und vorrichtung zum sortieren von teilchen
DE2732272B1 (de) * 1977-07-16 1978-10-26 Deutsches Krebsforsch Verfahren und Vorrichtung zur Fluoreszenzanalyse von gefaerbten Partikeln,insbesondere biologischen Zellen
DE3310665A1 (de) * 1982-03-25 1983-10-06 Becton Dickinson Co Vorrichtung zur messung von lichtoptischen parametern von partikeln

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Bergmann-Schaefer: Lehrbuch der Experimentalphysik, Bd. III, Optik, 7. Auflage, Walter de Gruyter, Berlin-New York, 1978, S. 49-51 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987005108A1 (en) * 1986-02-12 1987-08-27 Combustion Engineering, Inc. An in situ particle size measuring device cross-reference to related applications

Also Published As

Publication number Publication date
US4545677A (en) 1985-10-08
JPS60195436A (ja) 1985-10-03
DE3507407C2 (enExample) 1988-11-24
JPH0258585B2 (enExample) 1990-12-10

Similar Documents

Publication Publication Date Title
DE3507407A1 (de) Durchfluss-zytometriegeraet
DE3310665C2 (de) Vorrichtung zur Fluoreszenzanalyse
DE69830598T2 (de) Optische vorrichtung und methode
DE3822310C2 (enExample)
DE69032497T2 (de) Gerät zur Zählung von in einer Flüssigkeit suspendierten Teilchen
DE60218074T2 (de) Durchflusszytometer
DE69434366T2 (de) Verfahren und vorrichtung zur messung der teilchengrössenverteilung unter verwendung von laserstreuung
DE102011055294B4 (de) Mikroskopische Einrichtung und Verfahren zur dreidimensionalen Lokalisierung von punktförmigen Objekten in einer Probe
DE69631714T2 (de) Vorrichtung zur optischen Untersuchung eines Fluids, insbesondere zur hämatologischen Analyse
DE3513035C2 (enExample)
DE3937851A1 (de) Laser-doppler-geschwindigkeitsmesser
DE3000034A1 (de) Verfahren und vorrichtung zum messen der richtungsverteilungseigenschaften der von einem teilchen zurueckgestrahlten strahlungsenergie
DE19639939A1 (de) Optische Spektralmeßvorrichtung
DE69427067T2 (de) Streulichtintensitätsdetektor für von Filmen in kolloidalen Medien gestreutes Licht
DE3712153C1 (de) Verfahren zur Messung von Stroemungsvektoren in Gasstroemungen
DE19632829A1 (de) Verfahren und Vorrichtung zur Bestimmung von Linsenparametern
DE2922643C2 (enExample)
DE102016011568A1 (de) Schnelle Strahlvermessung in mehreren Ebenen
DE2608176C2 (enExample)
DE3145987A1 (de) "verfahren und vorrichtung zur messung der stroemungsvektoren in gasstroemungen"
DE4006618C2 (de) Vorrichtung zur Auskoppelung einer Meßstrahlung aus einem Laserstrahl
DE69711808T2 (de) Vorrichtung zur Aufnahme oder Wiedergabe und Verfahren zur Erkennung des Fokussierungszustandes
EP1434977A1 (de) Scatterometrische messanordnung und messverfahren
DE3338351A1 (de) Vorrichtung zur optischen erkennung von individuellen vielparametrischen eigenschaften von teilchen
EP3786613B1 (de) Partikelgrössenmessgerät

Legal Events

Date Code Title Description
OP8 Request for examination as to paragraph 44 patent law
D2 Grant after examination
8364 No opposition during term of opposition