DE3382554D1 - Verfahren zur herstellung von polypeptiden unter verwendung eines geladenen aminosaeurepolymers und einer exopeptidase. - Google Patents

Verfahren zur herstellung von polypeptiden unter verwendung eines geladenen aminosaeurepolymers und einer exopeptidase.

Info

Publication number
DE3382554D1
DE3382554D1 DE8888106450T DE3382554T DE3382554D1 DE 3382554 D1 DE3382554 D1 DE 3382554D1 DE 8888106450 T DE8888106450 T DE 8888106450T DE 3382554 T DE3382554 T DE 3382554T DE 3382554 D1 DE3382554 D1 DE 3382554D1
Authority
DE
Germany
Prior art keywords
exopeptidase
amino acid
acid polymer
charged amino
producing polypeptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8888106450T
Other languages
English (en)
Inventor
Stephen James Brewer
Helmut Max Sassenfeld
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GD Searle LLC
Original Assignee
GD Searle LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GD Searle LLC filed Critical GD Searle LLC
Application granted granted Critical
Publication of DE3382554D1 publication Critical patent/DE3382554D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/12—Growth hormone, growth factor other than t-cell or b-cell growth factor, and growth hormone releasing factor; related peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE8888106450T 1982-03-19 1983-03-17 Verfahren zur herstellung von polypeptiden unter verwendung eines geladenen aminosaeurepolymers und einer exopeptidase. Expired - Fee Related DE3382554D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8208051 1982-03-19

Publications (1)

Publication Number Publication Date
DE3382554D1 true DE3382554D1 (de) 1992-06-04

Family

ID=10529119

Family Applications (2)

Application Number Title Priority Date Filing Date
DE8888106450T Expired - Fee Related DE3382554D1 (de) 1982-03-19 1983-03-17 Verfahren zur herstellung von polypeptiden unter verwendung eines geladenen aminosaeurepolymers und einer exopeptidase.
DE8383102641T Expired DE3380260D1 (en) 1982-03-19 1983-03-17 Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE8383102641T Expired DE3380260D1 (en) 1982-03-19 1983-03-17 Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase

Country Status (5)

Country Link
US (1) US4532207A (de)
EP (2) EP0089626B1 (de)
JP (1) JPS58216697A (de)
CA (1) CA1195627A (de)
DE (2) DE3382554D1 (de)

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US4532207A (en) * 1982-03-19 1985-07-30 G. D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
AU2331784A (en) * 1982-12-10 1984-07-05 Nordisk Insulinlaboratorium Fremgangsmade til fremstilling af modne proteiner ud fra fusionsproteiner, syntetiseret i pro-eller eukaryote celler
DK55685A (da) * 1985-02-07 1986-08-08 Nordisk Gentofte Enzym eller enzymkompleks med proteolytisk aktivitet
US5618697A (en) * 1982-12-10 1997-04-08 Novo Nordisk A/S Process for preparing a desired protein
US4703004A (en) * 1984-01-24 1987-10-27 Immunex Corporation Synthesis of protein with an identification peptide
GB8412517D0 (en) * 1984-05-16 1984-06-20 Nagai K Recombinant fusion proteins
DE3572843D1 (en) * 1984-05-24 1989-10-12 Merck & Co Inc Site-specific proteolysis by renin
US6022950A (en) * 1984-06-07 2000-02-08 Seragen, Inc. Hybrid molecules having translocation region and cell-binding region
US5668255A (en) * 1984-06-07 1997-09-16 Seragen, Inc. Hybrid molecules having translocation region and cell-binding region
JP2554459B2 (ja) * 1984-07-02 1996-11-13 アース製薬 株式会社 β−ウロガストロン遺伝子、対応プラスミド組換体及び対応形質転換体
US5055399A (en) * 1985-01-15 1991-10-08 Genentech, Inc. Enzymatic synthesis of alpha-L-aspartyl-L-phenyalanine lower alkyl esters
US5753475A (en) * 1985-01-17 1998-05-19 Calgene, Inc. Methods and compositions for regulated transcription and expression of heterologous genes
US6281410B1 (en) 1986-07-31 2001-08-28 Calgene Llc Methods and compositions for regulated transcription and expression of heterologous genes
GB8504522D0 (en) * 1985-02-21 1985-03-27 Genetics Int Inc Electrochemistry of mediators
JPS61264000A (ja) * 1985-03-21 1986-11-21 イミユネツクス　コ−ポレイシヨン 標識ペプチドによるタンパク質の合成
GB8507666D0 (en) * 1985-03-25 1985-05-01 Wellcome Found Epidermal growth factor production
CA1260858A (en) * 1985-03-28 1989-09-26 Lawrence S. Cousens Expression using fused genes providing for protein product
US4751180A (en) * 1985-03-28 1988-06-14 Chiron Corporation Expression using fused genes providing for protein product
US5342921A (en) * 1985-03-28 1994-08-30 Chiron Corporation Superoxide dismutase fusion polypeptides for expression of mammalian proteins
WO1986007380A1 (en) * 1985-06-04 1986-12-18 Takeda Chemical Industries, Ltd. Process for preparing polypeptide
EP0207044A1 (de) * 1985-06-20 1986-12-30 Monsanto Company Freisetzung von Peptiden aus Polypeptiden
US4845196A (en) * 1985-06-24 1989-07-04 G. D. Searle & Co. Modified interferon gammas
EP0247906B1 (de) * 1986-02-04 1994-12-28 Mizuno, Den'Ichi Für Antitumor-Polypeptide kodierende DNS, die Polypeptide und diese Polypeptide enthaltenden Antitumor-Wirkstoffe
US5010010A (en) * 1986-10-22 1991-04-23 Selmer-Sande, A.S. Production of human parathyroid hormone from microorganisms
EP0604706A1 (de) 1986-10-22 1994-07-06 GAUTVIK, Kaare M., Dr. Herstellung von humanem Parathormon mittels Mikroorganismen
US5420242A (en) * 1986-10-22 1995-05-30 Kaare M. Gautvik Production of human parathyroid hormone from microorganisms
US4851341A (en) * 1986-12-19 1989-07-25 Immunex Corporation Immunoaffinity purification system
FR2615528B1 (fr) * 1987-05-18 1991-05-24 Pasteur Institut Procede de production d'un polypeptide ayant des proprietes choisies determinees, dans un organisme-hote transforme par un adn recombinant contenant des sequences nucleiques codant pour ce polypeptide et pour une proteine affine d'ose
EP0312346B1 (de) * 1987-10-16 1994-08-17 Genencor International, Inc. Sequenzspezifische saure E.coli-Protease
IT1223577B (it) * 1987-12-22 1990-09-19 Eniricerche Spa Procedimento migliorato per la preparazione dell'ormone della crescita umano naturale in forma pura
EP0335400B1 (de) * 1988-03-30 1994-06-08 Hitachi, Ltd. Verfahren zur Herstellung von Epidermal-Wachstumsfaktor durch genetische Transformation
US5156968A (en) * 1988-06-24 1992-10-20 Genentech, Inc. Purified yeast ubiquitin hydrolase
SE8802939D0 (sv) * 1988-08-19 1988-08-19 Kabigen Ab A method for the production and isolation of a fusion protein in eukaryotic cells
US5279954A (en) * 1989-06-30 1994-01-18 Board Of Regents Of The University Of Nebraska And Bionebraska Exopeptidase catalyzed site-specific bonding of supports, labels and bioactive agents to proteins
US5158875A (en) * 1989-08-25 1992-10-27 Amgen Inc. Production of biologically active insulin-like growth factor i from high expression host cell systems
JPH04503610A (ja) * 1990-01-19 1992-07-02 ベーリンガー　マンハイム　ゲゼルシャフト　ミット　ベシュレンクテル　ハフツング 基質を酵素学的に処理する方法
DE4039415A1 (de) 1990-02-03 1991-08-08 Boehringer Mannheim Gmbh Verfahren zur herstellung rekombinanter proteine ohne n-terminalen methioninrest
US5202239A (en) * 1990-08-07 1993-04-13 Scios Nova Inc. Expression of recombinant polypeptides with improved purification
SE9003534D0 (sv) * 1990-11-06 1990-11-06 Kabigen Ab A method for isolating and purifying peptides and proteins
DE4105480A1 (de) * 1991-02-21 1992-08-27 Boehringer Mannheim Gmbh Verbesserte aktivierung von rekombinanten proteinen
EP0559175B1 (de) * 1992-03-03 1999-07-14 Meiji Seika Kaisha Ltd. Gamma-Polyglutaminsäure-Hydrolase und Verfahren zu ihrer Herstellung
ATE247173T1 (de) * 1997-01-23 2003-08-15 Xzillion Gmbh & Co Kg Charakterisierung von polypeptiden
ES2210761T3 (es) 1997-05-21 2004-07-01 The Board Of Trustees Of The Leland Stanford Junior University Composicion y procedimiento para mejorar el transporte a traves de las membranas biologicas.
SE9702401D0 (sv) 1997-06-19 1997-06-19 Astra Ab Pharmaceutical use
US6958148B1 (en) * 1998-01-20 2005-10-25 Pericor Science, Inc. Linkage of agents to body tissue using microparticles and transglutaminase
US6919076B1 (en) 1998-01-20 2005-07-19 Pericor Science, Inc. Conjugates of agents and transglutaminase substrate linking molecules
US6730293B1 (en) 1999-08-24 2004-05-04 Cellgate, Inc. Compositions and methods for treating inflammatory diseases of the skin
US6669951B2 (en) 1999-08-24 2003-12-30 Cellgate, Inc. Compositions and methods for enhancing drug delivery across and into epithelial tissues
US7229961B2 (en) * 1999-08-24 2007-06-12 Cellgate, Inc. Compositions and methods for enhancing drug delivery across and into ocular tissues
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US6379903B1 (en) 1999-10-08 2002-04-30 Sigma-Aldrich Co. Purification of recombinant proteins fused to multiple epitopes
KR20020014459A (ko) * 2000-08-18 2002-02-25 서진호 양이온성 아미노산이 부가된 융합단백질 및 이를 이용한생물공정의 개선방법
EP1417237B1 (de) * 2001-07-26 2011-04-06 Advanced Protein Technologies Corp. Verfahren zur herstellung von interessanten polypeptiden aus fusionspolypeptiden
US6902914B2 (en) * 2001-09-28 2005-06-07 Sigma-Aldrich, Co. Recombinant DNA processes using a dNTP mixture containing modified nucleotides
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WO2008031020A2 (en) * 2006-09-08 2008-03-13 Wyeth Arginine wash in protein purification using affinity chromatography
CN116333157B (zh) * 2022-07-20 2023-10-03 无锡佰翱得生物科学股份有限公司 一种改造的d-阿洛酮糖3-差向异构酶及其应用

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Also Published As

Publication number Publication date
EP0306610B1 (de) 1992-04-29
EP0089626A3 (en) 1985-11-21
EP0089626A2 (de) 1983-09-28
US4532207A (en) 1985-07-30
DE3380260D1 (en) 1989-08-31
JPS58216697A (ja) 1983-12-16
EP0089626B1 (de) 1989-07-26
CA1195627A (en) 1985-10-22
EP0306610A1 (de) 1989-03-15

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8339 Ceased/non-payment of the annual fee