DE3221730A1 - Verfahren und analytische mittel zur aktivitaetsbestimmung von glutamat-oxalacetat-transaminase - Google Patents
Verfahren und analytische mittel zur aktivitaetsbestimmung von glutamat-oxalacetat-transaminaseInfo
- Publication number
- DE3221730A1 DE3221730A1 DE19823221730 DE3221730A DE3221730A1 DE 3221730 A1 DE3221730 A1 DE 3221730A1 DE 19823221730 DE19823221730 DE 19823221730 DE 3221730 A DE3221730 A DE 3221730A DE 3221730 A1 DE3221730 A1 DE 3221730A1
- Authority
- DE
- Germany
- Prior art keywords
- mmol
- chromogen
- imidazole
- alanine
- pyruvate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000034 method Methods 0.000 title claims abstract description 27
- 230000000694 effects Effects 0.000 title claims abstract description 18
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Substances C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims abstract description 13
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims abstract description 11
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 claims abstract description 11
- 229930195712 glutamate Natural products 0.000 claims abstract description 10
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 9
- 102000003992 Peroxidases Human genes 0.000 claims abstract description 9
- 108010042687 Pyruvate Oxidase Proteins 0.000 claims abstract description 9
- 108040007629 peroxidase activity proteins Proteins 0.000 claims abstract description 9
- 239000010452 phosphate Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 8
- RAHZWNYVWXNFOC-UHFFFAOYSA-N Sulphur dioxide Chemical compound O=S=O RAHZWNYVWXNFOC-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000000872 buffer Substances 0.000 claims abstract description 5
- 239000000126 substance Substances 0.000 claims abstract description 5
- LIPOUNRJVLNBCD-UHFFFAOYSA-N acetyl dihydrogen phosphate Chemical compound CC(=O)OP(O)(O)=O LIPOUNRJVLNBCD-UHFFFAOYSA-N 0.000 claims abstract description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 3
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 3
- 239000001301 oxygen Substances 0.000 claims abstract description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract 8
- 239000003795 chemical substances by application Substances 0.000 claims abstract 6
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract 4
- 239000007864 aqueous solution Substances 0.000 claims abstract 2
- 239000001569 carbon dioxide Substances 0.000 claims abstract 2
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract 2
- 238000012360 testing method Methods 0.000 claims description 28
- 238000006243 chemical reaction Methods 0.000 claims description 22
- KHPXUQMNIQBQEV-UHFFFAOYSA-N oxaloacetic acid Chemical compound OC(=O)CC(=O)C(O)=O KHPXUQMNIQBQEV-UHFFFAOYSA-N 0.000 claims description 16
- 102000004190 Enzymes Human genes 0.000 claims description 15
- 108090000790 Enzymes Proteins 0.000 claims description 15
- ADVPTQAUNPRNPO-UHFFFAOYSA-N 2-azaniumyl-3-sulfinopropanoate Chemical compound OC(=O)C(N)CS(O)=O ADVPTQAUNPRNPO-UHFFFAOYSA-N 0.000 claims description 14
- 239000003153 chemical reaction reagent Substances 0.000 claims description 11
- VWWQXMAJTJZDQX-UYBVJOGSSA-N flavin adenine dinucleotide Chemical compound C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1CO[P@](O)(=O)O[P@@](O)(=O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C2=NC(=O)NC(=O)C2=NC2=C1C=C(C)C(C)=C2 VWWQXMAJTJZDQX-UYBVJOGSSA-N 0.000 claims description 11
- 235000019162 flavin adenine dinucleotide Nutrition 0.000 claims description 11
- 239000011714 flavin adenine dinucleotide Substances 0.000 claims description 11
- 229940093632 flavin-adenine dinucleotide Drugs 0.000 claims description 11
- -1 3,5-dimethoxy-4-hydroxyphenyl Chemical group 0.000 claims description 10
- 239000000243 solution Substances 0.000 claims description 8
- 229960002363 thiamine pyrophosphate Drugs 0.000 claims description 8
- 235000008170 thiamine pyrophosphate Nutrition 0.000 claims description 8
- 239000011678 thiamine pyrophosphate Substances 0.000 claims description 8
- YXVCLPJQTZXJLH-UHFFFAOYSA-N thiamine(1+) diphosphate chloride Chemical compound [Cl-].CC1=C(CCOP(O)(=O)OP(O)(O)=O)SC=[N+]1CC1=CN=C(C)N=C1N YXVCLPJQTZXJLH-UHFFFAOYSA-N 0.000 claims description 8
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 6
- 102000003929 Transaminases Human genes 0.000 claims description 6
- 108090000340 Transaminases Proteins 0.000 claims description 6
- 238000006555 catalytic reaction Methods 0.000 claims description 5
- 159000000003 magnesium salts Chemical class 0.000 claims description 4
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims description 3
- 239000002250 absorbent Substances 0.000 claims description 3
- 230000002745 absorbent Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 2
- 239000007995 HEPES buffer Substances 0.000 claims description 2
- 239000007853 buffer solution Substances 0.000 claims description 2
- 229940053326 magnesium salt Drugs 0.000 claims 2
- 230000005540 biological transmission Effects 0.000 claims 1
- 125000000068 chlorophenyl group Chemical group 0.000 claims 1
- 239000000975 dye Substances 0.000 abstract description 9
- 239000002253 acid Substances 0.000 abstract description 3
- KPGXRSRHYNQIFN-UHFFFAOYSA-L 2-oxoglutarate(2-) Chemical compound [O-]C(=O)CCC(=O)C([O-])=O KPGXRSRHYNQIFN-UHFFFAOYSA-L 0.000 abstract 2
- SZXKSDXHODZTFS-UHFFFAOYSA-N 4-[4,5-bis[4-(dimethylamino)phenyl]-1H-imidazol-2-yl]-2,6-dimethoxyphenol Chemical compound COC1=C(O)C(OC)=CC(C=2NC(=C(N=2)C=2C=CC(=CC=2)N(C)C)C=2C=CC(=CC=2)N(C)C)=C1 SZXKSDXHODZTFS-UHFFFAOYSA-N 0.000 abstract 2
- 239000004291 sulphur dioxide Substances 0.000 abstract 1
- 235000010269 sulphur dioxide Nutrition 0.000 abstract 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 16
- 238000005259 measurement Methods 0.000 description 15
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 14
- 150000007857 hydrazones Chemical class 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 6
- 238000011534 incubation Methods 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 4
- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
- 230000008033 biological extinction Effects 0.000 description 3
- 239000007795 chemical reaction product Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000012954 diazonium Substances 0.000 description 3
- 150000001989 diazonium salts Chemical class 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 3
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 3
- JLTDJTHDQAWBAV-UHFFFAOYSA-N N,N-dimethylaniline Chemical compound CN(C)C1=CC=CC=C1 JLTDJTHDQAWBAV-UHFFFAOYSA-N 0.000 description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 239000003365 glass fiber Substances 0.000 description 2
- RNVCVTLRINQCPJ-UHFFFAOYSA-N o-toluidine Chemical compound CC1=CC=CC=C1N RNVCVTLRINQCPJ-UHFFFAOYSA-N 0.000 description 2
- 238000005691 oxidative coupling reaction Methods 0.000 description 2
- UIUJIQZEACWQSV-UHFFFAOYSA-N succinic semialdehyde Chemical compound OC(=O)CCC=O UIUJIQZEACWQSV-UHFFFAOYSA-N 0.000 description 2
- 210000001550 testis Anatomy 0.000 description 2
- YLUMOTIQYNIXLO-UHFFFAOYSA-N 1-morpholin-4-ylethanesulfonic acid Chemical compound OS(=O)(=O)C(C)N1CCOCC1 YLUMOTIQYNIXLO-UHFFFAOYSA-N 0.000 description 1
- SXGZJKUKBWWHRA-UHFFFAOYSA-N 2-(N-morpholiniumyl)ethanesulfonate Chemical compound [O-]S(=O)(=O)CC[NH+]1CCOCC1 SXGZJKUKBWWHRA-UHFFFAOYSA-N 0.000 description 1
- MOMFXATYAINJML-UHFFFAOYSA-N 2-Acetylthiazole Chemical group CC(=O)C1=NC=CS1 MOMFXATYAINJML-UHFFFAOYSA-N 0.000 description 1
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 description 1
- WDJHALXBUFZDSR-UHFFFAOYSA-N Acetoacetic acid Natural products CC(=O)CC(O)=O WDJHALXBUFZDSR-UHFFFAOYSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 208000029578 Muscle disease Diseases 0.000 description 1
- WVVOBOZHTQJXPB-UHFFFAOYSA-N N-anilino-N-nitronitramide Chemical compound [N+](=O)([O-])N(NC1=CC=CC=C1)[N+](=O)[O-] WVVOBOZHTQJXPB-UHFFFAOYSA-N 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 208000037273 Pathologic Processes Diseases 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001448 anilines Chemical class 0.000 description 1
- 229940009098 aspartate Drugs 0.000 description 1
- 239000000987 azo dye Substances 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- WOWBFOBYOAGEEA-UHFFFAOYSA-N diafenthiuron Chemical compound CC(C)C1=C(NC(=S)NC(C)(C)C)C(C(C)C)=CC(OC=2C=CC=CC=2)=C1 WOWBFOBYOAGEEA-UHFFFAOYSA-N 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000003748 differential diagnosis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000011152 fibreglass Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 125000001072 heteroaryl group Chemical group 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 239000012434 nucleophilic reagent Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000009054 pathological process Effects 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920006289 polycarbonate film Polymers 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000001603 reducing effect Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000007086 side reaction Methods 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 125000003831 tetrazolyl group Chemical group 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/52—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving transaminase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/805—Test papers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19823221730 DE3221730A1 (de) | 1982-06-09 | 1982-06-09 | Verfahren und analytische mittel zur aktivitaetsbestimmung von glutamat-oxalacetat-transaminase |
| US06/500,111 US4591553A (en) | 1982-06-09 | 1983-06-01 | Process and analytical agent for the determination of the activity of glutamate-oxalacetate-transaminase |
| JP58097034A JPS58220696A (ja) | 1982-06-09 | 1983-06-02 | グルタメ−ト−オキサロアセテ−ト−トランスアミナ−ゼの活性測定法及びそのための分析試薬及び分析試験片 |
| AT83105503T ATE17872T1 (de) | 1982-06-09 | 1983-06-03 | Verfahren und analytische mittel zur aktivitaetsbestimmung von glutamat-oxalacetattransaminase. |
| EP83105503A EP0100413B1 (de) | 1982-06-09 | 1983-06-03 | Verfahren und analytische Mittel zur Aktivitätsbestimmung von Glutamat-Oxalacetat-Transaminase |
| DE8383105503T DE3362076D1 (en) | 1982-06-09 | 1983-06-03 | Method and preparation for the determination of glutamic-oxalacetic transaminase activity |
| DD83251806A DD209848A5 (de) | 1982-06-09 | 1983-06-07 | Verfahren zur aktivitaetsbestimmung von glutamat-oxalacetat-transaminase |
| ZA834166A ZA834166B (en) | 1982-06-09 | 1983-06-08 | Process and analytical agent for the determination of the enzyme glutamate-oxalacetate-transaminase |
| IE1351/83A IE55265B1 (en) | 1982-06-09 | 1983-06-08 | Process and analytical agent for the determination of the enzyme glutamate-oxalacetate-transaminase |
| CA000429972A CA1206401A (en) | 1982-06-09 | 1983-06-08 | Process and analytical agent for the determination of the enzyme glutamate-oxalacetate-transaminase |
| ES523127A ES8402877A1 (es) | 1982-06-09 | 1983-06-09 | "procedimiento para la determinacion de actividad de glutamato-oxalacetato-transaminasa" |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19823221730 DE3221730A1 (de) | 1982-06-09 | 1982-06-09 | Verfahren und analytische mittel zur aktivitaetsbestimmung von glutamat-oxalacetat-transaminase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE3221730A1 true DE3221730A1 (de) | 1983-12-15 |
Family
ID=6165690
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19823221730 Withdrawn DE3221730A1 (de) | 1982-06-09 | 1982-06-09 | Verfahren und analytische mittel zur aktivitaetsbestimmung von glutamat-oxalacetat-transaminase |
| DE8383105503T Expired DE3362076D1 (en) | 1982-06-09 | 1983-06-03 | Method and preparation for the determination of glutamic-oxalacetic transaminase activity |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE8383105503T Expired DE3362076D1 (en) | 1982-06-09 | 1983-06-03 | Method and preparation for the determination of glutamic-oxalacetic transaminase activity |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US4591553A (cg-RX-API-DMAC7.html) |
| EP (1) | EP0100413B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JPS58220696A (cg-RX-API-DMAC7.html) |
| AT (1) | ATE17872T1 (cg-RX-API-DMAC7.html) |
| CA (1) | CA1206401A (cg-RX-API-DMAC7.html) |
| DD (1) | DD209848A5 (cg-RX-API-DMAC7.html) |
| DE (2) | DE3221730A1 (cg-RX-API-DMAC7.html) |
| ES (1) | ES8402877A1 (cg-RX-API-DMAC7.html) |
| IE (1) | IE55265B1 (cg-RX-API-DMAC7.html) |
| ZA (1) | ZA834166B (cg-RX-API-DMAC7.html) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0523227A4 (en) * | 1991-01-31 | 1993-10-20 | Xytronyx, Inc. | One-step test for aspartate aminotransferase |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5859113A (en) | 1997-07-17 | 1999-01-12 | E. I. Du Pont De Nemours And Company | Pigment dispersions containing hydroxylated AB-block polymer dispersant |
| CN102288747B (zh) * | 2011-08-19 | 2013-11-27 | 长沙三诺生物传感技术股份有限公司 | 去除干扰的干化学定量测试试条、谷丙或谷草转氨酶定量测试试条及检测方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3701601A (en) * | 1968-03-04 | 1972-10-31 | Sherwood Medical Ind Inc | Photometric read out and analyzing system |
| US3875014A (en) * | 1973-12-14 | 1975-04-01 | American Cyanamid Co | Glutamic oxaloacetic transaminase test material |
| US4089747A (en) * | 1976-08-09 | 1978-05-16 | Eastman Kodak Company | Compositions for the detection of hydrogen peroxide |
| US4184923A (en) * | 1977-11-03 | 1980-01-22 | Eastman Kodak Company | Reduction of gentisic acid interference in analytical elements |
| US4246342A (en) * | 1978-03-25 | 1981-01-20 | Toyo Jozo Kabushiki Kaisha | Process for the manufacture of pyruvate oxidase, and analytical method and kit for the use of the same |
| DE2834706A1 (de) * | 1978-08-08 | 1980-02-14 | Boehringer Mannheim Gmbh | Verfahren und reagens zur bestimmung von glutamat-oxalacetat-transaminase und glutamat-pyruvat-transaminase |
| IT1162349B (it) * | 1979-07-17 | 1987-03-25 | Biodata Spa | Metodo per la determinazione delle transaminasi e relativo kit diagnostico |
-
1982
- 1982-06-09 DE DE19823221730 patent/DE3221730A1/de not_active Withdrawn
-
1983
- 1983-06-01 US US06/500,111 patent/US4591553A/en not_active Expired - Lifetime
- 1983-06-02 JP JP58097034A patent/JPS58220696A/ja active Granted
- 1983-06-03 EP EP83105503A patent/EP0100413B1/de not_active Expired
- 1983-06-03 AT AT83105503T patent/ATE17872T1/de not_active IP Right Cessation
- 1983-06-03 DE DE8383105503T patent/DE3362076D1/de not_active Expired
- 1983-06-07 DD DD83251806A patent/DD209848A5/de not_active IP Right Cessation
- 1983-06-08 IE IE1351/83A patent/IE55265B1/en not_active IP Right Cessation
- 1983-06-08 ZA ZA834166A patent/ZA834166B/xx unknown
- 1983-06-08 CA CA000429972A patent/CA1206401A/en not_active Expired
- 1983-06-09 ES ES523127A patent/ES8402877A1/es not_active Expired
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0523227A4 (en) * | 1991-01-31 | 1993-10-20 | Xytronyx, Inc. | One-step test for aspartate aminotransferase |
| US5834226A (en) * | 1991-01-31 | 1998-11-10 | Xytronyx, Inc. | One-step test for aspartate aminotransferase |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3362076D1 (en) | 1986-03-20 |
| ZA834166B (en) | 1984-03-28 |
| IE831351L (en) | 1983-12-09 |
| ATE17872T1 (de) | 1986-02-15 |
| ES523127A0 (es) | 1984-03-16 |
| DD209848A5 (de) | 1984-05-23 |
| CA1206401A (en) | 1986-06-24 |
| ES8402877A1 (es) | 1984-03-16 |
| IE55265B1 (en) | 1990-07-18 |
| JPS58220696A (ja) | 1983-12-22 |
| JPH0368679B2 (cg-RX-API-DMAC7.html) | 1991-10-29 |
| US4591553A (en) | 1986-05-27 |
| EP0100413B1 (de) | 1986-02-05 |
| EP0100413A1 (de) | 1984-02-15 |
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|---|---|---|---|
| 8139 | Disposal/non-payment of the annual fee |