DE3072111D1 - Assay method using enzymes as labeling substances - Google Patents

Assay method using enzymes as labeling substances

Info

Publication number
DE3072111D1
DE3072111D1 DE8080303478T DE3072111T DE3072111D1 DE 3072111 D1 DE3072111 D1 DE 3072111D1 DE 8080303478 T DE8080303478 T DE 8080303478T DE 3072111 T DE3072111 T DE 3072111T DE 3072111 D1 DE3072111 D1 DE 3072111D1
Authority
DE
Germany
Prior art keywords
enzyme
modulator
enzymes
ligand
receptor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE8080303478T
Other languages
English (en)
Inventor
Colin Henry Self
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of DE3072111D1 publication Critical patent/DE3072111D1/de
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/966—Chemistry: molecular biology and microbiology involving an enzyme system with high turnover rate or complement magnified assay, e.g. multi-enzyme systems
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE8080303478T 1979-10-03 1980-10-02 Assay method using enzymes as labeling substances Expired DE3072111D1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB7934376A GB2059421A (en) 1979-10-03 1979-10-03 Assay method and reagents therefor

Publications (1)

Publication Number Publication Date
DE3072111D1 true DE3072111D1 (en) 1988-09-15

Family

ID=10508272

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8080303478T Expired DE3072111D1 (en) 1979-10-03 1980-10-02 Assay method using enzymes as labeling substances

Country Status (9)

Country Link
US (2) US4598042A (de)
EP (1) EP0027036B1 (de)
JP (1) JPS56501463A (de)
AT (1) ATE36413T1 (de)
AU (1) AU544496B2 (de)
DE (1) DE3072111D1 (de)
DK (1) DK165382C (de)
GB (1) GB2059421A (de)
WO (1) WO1981001057A1 (de)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2059421A (en) * 1979-10-03 1981-04-23 Self C H Assay method and reagents therefor
US4446231A (en) * 1979-10-03 1984-05-01 Self Colin H Immunoassay using an amplified cyclic detection system
EP0049606A1 (de) * 1980-10-02 1982-04-14 Colin Henry Self Nachweisverfahren, Anwendung und diagnostisches Hilfsmittel
EP0060123B1 (de) * 1981-03-07 1986-10-29 Colin Henry Self Test und seine Verwendung
US4543339A (en) * 1982-03-16 1985-09-24 Oneill Christopher Early pregnancy detection by detecting enhanced blood platelet activation
US4517303A (en) * 1982-10-20 1985-05-14 E. I. Du Pont De Nemours And Company Specific binding assays utilizing analyte-cytolysin conjugates
JPS5982398A (ja) * 1982-11-01 1984-05-12 Toyobo Co Ltd 補酵素の安定化法
JPS59140900A (ja) * 1983-01-28 1984-08-13 Toyo Jozo Co Ltd 新規な酵素的高感度測定法
US4699876A (en) * 1984-01-27 1987-10-13 E. I. Du Pont De Nemours And Company Nonradiometric polynucleotide probes
GB8407736D0 (en) * 1984-03-26 1984-05-02 Univ London Detecting specific polynucleotide sequences
GB8427006D0 (en) * 1984-10-25 1984-11-28 Iq Bio Ltd Determination of chlamydia trachomatis
US4767699A (en) * 1985-05-02 1988-08-30 Allied Corporation Diagnostic reagent, kit and method employing polynucleotide displacement, separation, enzymatic cleavage and adenosine phosphate detection
US4795701A (en) * 1985-07-17 1989-01-03 Allied Corporation Homogeneous polynucleotide displacement assay method kit and reagent complex
US4792521A (en) * 1985-08-15 1988-12-20 Immunomedics, Inc. Non-enzymatic immunohistochemical staining system and reagents
US5190864A (en) * 1986-04-15 1993-03-02 Northeastern University Enzyme amplification by using free enzyme to release enzyme from an immobilized enzyme material
US4937188A (en) * 1986-04-15 1990-06-26 Northeastern University Enzyme activity amplification method for increasing assay sensitivity
US4810631A (en) * 1986-05-12 1989-03-07 Becton, Dickinson And Company Signal enhancement in immunoassay by modulation of chemical catalysis
US4835099A (en) * 1986-11-20 1989-05-30 Becton, Dickinson And Company Signal enhancement in immunoassay by modulation of enzymatic catalysis
US4847194A (en) * 1987-03-13 1989-07-11 Becton, Dickinson And Company Colorimetric detection of delta-5-3-ketosteroid isomerase and immunoassay based thereon
IL85597A (en) * 1987-05-18 1992-08-18 Technicon Instr Immunoassay method and composition using conjugate of analyte and reaction intermediate for analyte capable of mediating signal generation
US4829519A (en) * 1987-06-09 1989-05-09 Scotton Geoffrey R Automatic cell transfer system with error rate assessment
EP0304934A3 (de) * 1987-08-26 1989-07-26 Hunger, Hans-Dieter Markierte molekulare Sonden, Verfahren zu ihrer Herstellung und ihre Verwendung
US4943525A (en) * 1987-11-02 1990-07-24 Bioventures, Inc. Simultaneous immunoassay for the determination of antigens and antibodies
IE900688A1 (en) * 1989-03-08 1991-02-13 Becton Dickinson Co Signal enhancement in magnetic immunoassay by inhibition of¹enzymatic catalysis
US5196306A (en) * 1989-03-29 1993-03-23 E. I. Du Pont De Nemours And Company Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system
JP2977895B2 (ja) * 1989-10-17 1999-11-15 ブリティッシュ・テクノロジー・グループ・リミテッド 増幅化学ルミネセントアッセイ
JPH0673478B2 (ja) * 1990-01-11 1994-09-21 旭化成工業株式会社 Ｌ―カルニチンの高感度測定法および測定用組成物
JP2818695B2 (ja) * 1991-01-25 1998-10-30 財団法人野田産業科学研究所 Ｎａｄｈキナーゼ及びその製造法
JP2818696B2 (ja) * 1991-01-25 1998-10-30 財団法人野田産業科学研究所 Ｎａｄｈキナーゼを用いる高感度定量法
JPH06509646A (ja) * 1991-07-26 1994-10-27 デイド・ケミストリイ・システムズ・インコーポレーテツド 懸濁された固体支持体の存在下でのシグナル検出アッセイ
DE69320224T2 (de) * 1992-03-17 1999-05-06 Unitika Ltd Verfahren und Reagenz zur enzymatischen Analyse
US5279937A (en) * 1992-04-30 1994-01-18 Detechnology Canada Use of macroglobulins to improve the signal-to-background ratio in affinity binding assays
DE4217474A1 (de) * 1992-05-27 1993-12-02 Boehringer Mannheim Gmbh Verfahren und Mittel zur Bestimmung eines Analyts
JPH06153991A (ja) * 1992-11-18 1994-06-03 Unitika Ltd ホスファターゼ測定試薬
US5843696A (en) * 1995-11-01 1998-12-01 Wisconsin Alumni Research Foundation Bioassay for toxic substances activated by metabolic enzyme system
US5804403A (en) * 1997-01-31 1998-09-08 Boehringer Mannheim Corporation Stable aqueous reagent containing NAD
US6248552B1 (en) * 1997-10-24 2001-06-19 Birkmayer Pharmaceuticals Enzyme-based assay for determining effects of exogenous and endogenous factors on cellular energy production
US6136339A (en) * 1998-08-21 2000-10-24 Gardiner; Paul T. Food supplements and methods comprising lipoic acid and creatine
JP3395673B2 (ja) * 1998-11-18 2003-04-14 株式会社豊田中央研究所 微小酸素電極を利用した特異的結合対測定方法
DE19911777A1 (de) * 1999-03-17 2000-09-21 Merck Patent Gmbh Verfahren zur Herstellung von kosmetischen Formulierungen
WO2007033171A2 (en) * 2005-09-12 2007-03-22 Research & Diagnostic Systems, Inc. Mycoplasma detection method and composition
CN101339167B (zh) * 2008-08-27 2011-12-14 中国药科大学 基于靶蛋白亲和选择的活性成分高通量筛选方法
CN114908139B (zh) * 2022-07-15 2022-10-04 北京索莱宝科技有限公司 线粒体异柠檬酸脱氢酶的活性检测系统及其应用

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3654090A (en) * 1968-09-24 1972-04-04 Organon Method for the determination of antigens and antibodies
NL154600B (nl) * 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
US3730844A (en) * 1971-08-27 1973-05-01 Purdue Research Foundation Polynucleotide analysis
NL171930C (nl) * 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
IN142734B (de) * 1975-04-28 1977-08-20 Miles Lab
US4230797A (en) * 1975-04-28 1980-10-28 Miles Laboratories, Inc. Heterogenous specific binding assay employing a coenzyme as label
US4166767A (en) * 1976-03-25 1979-09-04 Dainippon Pharmaceutical Co., Ltd. Insolubilized antibody
JPS5344622A (en) * 1976-09-30 1978-04-21 Mochida Pharm Co Ltd Immunologically measuring method
US4134792A (en) * 1976-12-06 1979-01-16 Miles Laboratories, Inc. Specific binding assay with an enzyme modulator as a labeling substance
GB1595101A (en) * 1977-03-15 1981-08-05 Hoffmann La Roche Enzyme modifier immunoassay
IT1105734B (it) * 1977-07-14 1985-11-04 Syva Co Prova di legame di competizione di antienzima omogeneo
US4233402A (en) * 1978-04-05 1980-11-11 Syva Company Reagents and method employing channeling
US4318980A (en) * 1978-04-10 1982-03-09 Miles Laboratories, Inc. Heterogenous specific binding assay employing a cycling reactant as label
JPS5510590A (en) * 1978-05-04 1980-01-25 Wellcome Found Enzyme immunity quantity analysis
US4193983A (en) * 1978-05-16 1980-03-18 Syva Company Labeled liposome particle compositions and immunoassays therewith
US4277560A (en) * 1978-10-24 1981-07-07 Technicon Instruments Corporation Enzyme immunoassays using immobilized reagents in a flowing stream
IL55816A (en) * 1978-10-30 1982-04-30 Ames Yissum Ltd Method for simultaneous immunoassay of several different antibodies and a kit therefor
US4289748A (en) * 1979-05-31 1981-09-15 United States Of America Ultrasensitive enzymatic radioimmunoassay method
US4307188A (en) * 1979-09-06 1981-12-22 Miles Laboratories, Inc. Precursor indicator compositions
WO1981000725A1 (en) * 1979-09-14 1981-03-19 Charles Hospital Dev Method of determining a substrate in a sample
US4446231A (en) * 1979-10-03 1984-05-01 Self Colin H Immunoassay using an amplified cyclic detection system
GB2059421A (en) * 1979-10-03 1981-04-23 Self C H Assay method and reagents therefor
US4299916A (en) * 1979-12-26 1981-11-10 Syva Company Preferential signal production on a surface in immunoassays
DE2952478A1 (de) * 1979-12-27 1981-07-30 Merck Patent Gmbh, 6100 Darmstadt Verfahren und mittel zur immunologischen bestimmung von enzymen

Also Published As

Publication number Publication date
JPS56501463A (de) 1981-10-08
DK165382B (da) 1992-11-16
AU544496B2 (en) 1985-05-30
ATE36413T1 (de) 1988-08-15
GB2059421A (en) 1981-04-23
DK242781A (da) 1981-06-02
US4769321A (en) 1988-09-06
WO1981001057A1 (en) 1981-04-16
EP0027036B1 (de) 1988-08-10
AU6337980A (en) 1981-04-28
EP0027036A1 (de) 1981-04-15
DK165382C (da) 1993-04-05
US4598042A (en) 1986-07-01

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition