DE2800934C2 - Verfahren zur Durchführung von Volumenmessungen an der Zwischenschicht zwischen der Erythrozytenschicht und der Plasmaschicht einer zentrifugierten Blutprobe - Google Patents
Verfahren zur Durchführung von Volumenmessungen an der Zwischenschicht zwischen der Erythrozytenschicht und der Plasmaschicht einer zentrifugierten BlutprobeInfo
- Publication number
- DE2800934C2 DE2800934C2 DE2800934A DE2800934A DE2800934C2 DE 2800934 C2 DE2800934 C2 DE 2800934C2 DE 2800934 A DE2800934 A DE 2800934A DE 2800934 A DE2800934 A DE 2800934A DE 2800934 C2 DE2800934 C2 DE 2800934C2
- Authority
- DE
- Germany
- Prior art keywords
- layer
- blood sample
- intermediate layer
- blood
- erythrocyte
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000003743 erythrocyte Anatomy 0.000 title claims description 33
- 210000004369 blood Anatomy 0.000 title claims description 27
- 239000008280 blood Substances 0.000 title claims description 27
- 238000000034 method Methods 0.000 title claims description 12
- 238000005259 measurement Methods 0.000 title claims description 3
- 239000000126 substance Substances 0.000 claims description 11
- 230000005484 gravity Effects 0.000 claims description 9
- 239000000654 additive Substances 0.000 claims description 8
- 230000000996 additive effect Effects 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 2
- 239000010410 layer Substances 0.000 claims 9
- 230000018044 dehydration Effects 0.000 claims 2
- 238000006297 dehydration reaction Methods 0.000 claims 2
- 238000006073 displacement reaction Methods 0.000 claims 2
- 239000011229 interlayer Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- IRXRGVFLQOSHOH-UHFFFAOYSA-L dipotassium;oxalate Chemical compound [K+].[K+].[O-]C(=O)C([O-])=O IRXRGVFLQOSHOH-UHFFFAOYSA-L 0.000 description 12
- 239000000523 sample Substances 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 210000000601 blood cell Anatomy 0.000 description 7
- 210000003714 granulocyte Anatomy 0.000 description 7
- 239000012528 membrane Substances 0.000 description 6
- 239000012503 blood component Substances 0.000 description 4
- 239000003146 anticoagulant agent Substances 0.000 description 3
- 229940127219 anticoagulant drug Drugs 0.000 description 3
- 239000000306 component Substances 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- 210000000265 leukocyte Anatomy 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000005345 coagulation Methods 0.000 description 2
- 230000015271 coagulation Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005534 hematocrit Methods 0.000 description 2
- GKQWYZBANWAFMQ-UHFFFAOYSA-M lithium;2-hydroxypropanoate Chemical compound [Li+].CC(O)C([O-])=O GKQWYZBANWAFMQ-UHFFFAOYSA-M 0.000 description 2
- 210000005087 mononuclear cell Anatomy 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- 244000215068 Acacia senegal Species 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 239000000205 acacia gum Substances 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 150000001457 metallic cations Chemical class 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 150000002891 organic anions Chemical class 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Clinical Laboratory Science (AREA)
- Ecology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US75806577A | 1977-01-10 | 1977-01-10 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE2800934A1 DE2800934A1 (de) | 1978-07-13 |
| DE2800934C2 true DE2800934C2 (de) | 1986-09-18 |
Family
ID=25050351
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2800934A Expired DE2800934C2 (de) | 1977-01-10 | 1978-01-10 | Verfahren zur Durchführung von Volumenmessungen an der Zwischenschicht zwischen der Erythrozytenschicht und der Plasmaschicht einer zentrifugierten Blutprobe |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US4159896A (cg-RX-API-DMAC7.html) |
| DE (1) | DE2800934C2 (cg-RX-API-DMAC7.html) |
| FR (1) | FR2377041A1 (cg-RX-API-DMAC7.html) |
| IT (1) | IT1103118B (cg-RX-API-DMAC7.html) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4310430A (en) * | 1979-09-11 | 1982-01-12 | Terumo Corporation | α-Olefin-dialkylmaleate-based liquid separating agent |
| ES8307122A1 (es) * | 1981-08-27 | 1983-06-16 | Becton Dickinson Co | "perfeccionamientos introducidos en un aparato para introducir reactivos en recipientes de recogida de muestras". |
| US4594165A (en) * | 1984-11-16 | 1986-06-10 | Levine Robert A | Method of enhancing separation of abnormally light red cells from granulocytes in a centrifuged blood sample |
| US4567754A (en) * | 1985-03-29 | 1986-02-04 | Wardlaw Stephen C | Measurement of small heavy constituent layer in stratified mixture |
| US5065768A (en) * | 1988-09-13 | 1991-11-19 | Safe-Tec Clinical Products, Inc. | Self-sealing fluid conduit and collection device |
| JPH0774772B2 (ja) * | 1990-12-31 | 1995-08-09 | エイ． レビン ロバート | 血液サンプリング組立体、ターゲット細胞の採取方法およびターゲット成分の採取方法 |
| US5252460A (en) * | 1991-10-28 | 1993-10-12 | Fiedler Paul N | In vitro detection of ova, parasites, and other formed elements in stool |
| US5342790A (en) * | 1992-10-30 | 1994-08-30 | Becton Dickinson And Company | Apparatus for indirect fluorescent assay of blood samples |
| WO1994029722A1 (en) * | 1993-06-08 | 1994-12-22 | Chronomed, Inc. | Two-phase optical assay method and apparatus |
| US5422018A (en) * | 1994-01-31 | 1995-06-06 | Applied Imaging | Centrifuge tube and adaptor |
| US5560830A (en) * | 1994-12-13 | 1996-10-01 | Coleman; Charles M. | Separator float and tubular body for blood collection and separation and method of use thereof |
| US5733545A (en) * | 1995-03-03 | 1998-03-31 | Quantic Biomedical Partners | Platelet glue wound sealant |
| JPH11508813A (ja) | 1995-06-06 | 1999-08-03 | クウォンティック バイオメディカル パートナーズ | 血漿を濃縮するための装置及び方法 |
| US5736033A (en) * | 1995-12-13 | 1998-04-07 | Coleman; Charles M. | Separator float for blood collection tubes with water swellable material |
| AU4101697A (en) * | 1996-11-22 | 1998-05-28 | Robert A. Levine | Process for enhancing the aggregation and/or agglutination of erythrocytes prior to centrifugation |
| US5811303A (en) * | 1997-04-01 | 1998-09-22 | Streck Laboratories, Inc. | Quantitative buffy coat control composition |
| US7947236B2 (en) | 1999-12-03 | 2011-05-24 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
| US7205157B2 (en) * | 2001-01-08 | 2007-04-17 | Becton, Dickinson And Company | Method of separating cells from a sample |
| US7374678B2 (en) | 2002-05-24 | 2008-05-20 | Biomet Biologics, Inc. | Apparatus and method for separating and concentrating fluids containing multiple components |
| US7832566B2 (en) | 2002-05-24 | 2010-11-16 | Biomet Biologics, Llc | Method and apparatus for separating and concentrating a component from a multi-component material including macroparticles |
| US20030205538A1 (en) * | 2002-05-03 | 2003-11-06 | Randel Dorian | Methods and apparatus for isolating platelets from blood |
| US7992725B2 (en) | 2002-05-03 | 2011-08-09 | Biomet Biologics, Llc | Buoy suspension fractionation system |
| US20060278588A1 (en) | 2002-05-24 | 2006-12-14 | Woodell-May Jennifer E | Apparatus and method for separating and concentrating fluids containing multiple components |
| DE10392686T5 (de) | 2002-05-24 | 2005-07-07 | Biomet Mfg. Corp., Warsaw | Vorrichtung und Verfahren zum Trennen und Konzentrieren von Flüssigkeiten, welche mehrere Komponenten enthalten |
| US7845499B2 (en) | 2002-05-24 | 2010-12-07 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
| US7074577B2 (en) | 2002-10-03 | 2006-07-11 | Battelle Memorial Institute | Buffy coat tube and float system and method |
| WO2006086199A1 (en) | 2005-02-07 | 2006-08-17 | Hanuman Llc | Platelet rich plasma concentrate apparatus and method |
| US7866485B2 (en) | 2005-02-07 | 2011-01-11 | Hanuman, Llc | Apparatus and method for preparing platelet rich plasma and concentrates thereof |
| US7708152B2 (en) | 2005-02-07 | 2010-05-04 | Hanuman Llc | Method and apparatus for preparing platelet rich plasma and concentrates thereof |
| US8567609B2 (en) | 2006-05-25 | 2013-10-29 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
| JP5479319B2 (ja) | 2007-04-12 | 2014-04-23 | バイオメット・バイオロジックス・リミテッド・ライアビリティ・カンパニー | ブイ式懸濁液分画システム |
| US8328024B2 (en) | 2007-04-12 | 2012-12-11 | Hanuman, Llc | Buoy suspension fractionation system |
| PL2259774T3 (pl) | 2008-02-27 | 2013-04-30 | Biomet Biologics Llc | Sposoby i kompozycje dla wprowadzania antagonisty receptora interleukiny-1 |
| EP2254991B1 (en) | 2008-02-29 | 2018-08-22 | Biomet Manufacturing, LLC | A system and process for separating a material |
| US8012077B2 (en) | 2008-05-23 | 2011-09-06 | Biomet Biologics, Llc | Blood separating device |
| EP2326421B1 (en) * | 2008-07-21 | 2012-06-20 | Becton, Dickinson and Company | Density phase separation device |
| AU2009274096B2 (en) | 2008-07-21 | 2012-08-02 | Becton, Dickinson And Company | Density phase separation device |
| ES2430638T3 (es) | 2008-07-21 | 2013-11-21 | Becton, Dickinson And Company | Dispositivo de separación de fases por densidad |
| US8177072B2 (en) | 2008-12-04 | 2012-05-15 | Thermogenesis Corp. | Apparatus and method for separating and isolating components of a biological fluid |
| US8187475B2 (en) | 2009-03-06 | 2012-05-29 | Biomet Biologics, Llc | Method and apparatus for producing autologous thrombin |
| US8313954B2 (en) | 2009-04-03 | 2012-11-20 | Biomet Biologics, Llc | All-in-one means of separating blood components |
| KR101393321B1 (ko) * | 2009-05-15 | 2014-05-14 | 벡톤 디킨슨 앤드 컴퍼니 | 밀도 상 분리 장치 |
| US9011800B2 (en) | 2009-07-16 | 2015-04-21 | Biomet Biologics, Llc | Method and apparatus for separating biological materials |
| US8591391B2 (en) | 2010-04-12 | 2013-11-26 | Biomet Biologics, Llc | Method and apparatus for separating a material |
| US8377395B2 (en) | 2010-04-29 | 2013-02-19 | Charles M. Coleman | Integrated blood specimen processor |
| US9642956B2 (en) | 2012-08-27 | 2017-05-09 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
| US9950035B2 (en) | 2013-03-15 | 2018-04-24 | Biomet Biologics, Llc | Methods and non-immunogenic compositions for treating inflammatory disorders |
| US10143725B2 (en) | 2013-03-15 | 2018-12-04 | Biomet Biologics, Llc | Treatment of pain using protein solutions |
| US9895418B2 (en) | 2013-03-15 | 2018-02-20 | Biomet Biologics, Llc | Treatment of peripheral vascular disease using protein solutions |
| US10208095B2 (en) | 2013-03-15 | 2019-02-19 | Biomet Manufacturing, Llc | Methods for making cytokine compositions from tissues using non-centrifugal methods |
| US20140271589A1 (en) | 2013-03-15 | 2014-09-18 | Biomet Biologics, Llc | Treatment of collagen defects using protein solutions |
| GB201306810D0 (en) | 2013-04-15 | 2013-05-29 | Cells4Life Group Llp | Methods of cell separation |
| US9694359B2 (en) | 2014-11-13 | 2017-07-04 | Becton, Dickinson And Company | Mechanical separator for a biological fluid |
| US9713810B2 (en) | 2015-03-30 | 2017-07-25 | Biomet Biologics, Llc | Cell washing plunger using centrifugal force |
| US9757721B2 (en) | 2015-05-11 | 2017-09-12 | Biomet Biologics, Llc | Cell washing plunger using centrifugal force |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2102785A (en) * | 1935-10-18 | 1937-12-21 | Brooks Clyde | Method of and apparatus for sedimentation testing of blood |
| US2725782A (en) * | 1952-08-19 | 1955-12-06 | Worley Carl Milton | Apparatus for recording rate of sedimentation in liquids |
| DE6945462U (de) * | 1969-11-17 | 1970-04-16 | Helmut Dr Niepage | Pcv-meter |
| US3684450A (en) * | 1970-09-14 | 1972-08-15 | Stanford L Adler | Automatic apparatus and method for determining the packed cell volume of whole blood |
| US3965889A (en) * | 1971-11-26 | 1976-06-29 | Commissariat A L'energie Atomique | Apparatus for the sampling of blood and the separation of plasma under anaerobic conditions |
| US3914985A (en) * | 1974-03-29 | 1975-10-28 | American Hospital Supply Corp | Centrifuging device and method |
| US3931018A (en) * | 1974-08-09 | 1976-01-06 | Becton, Dickinson And Company | Assembly for collection, separation and filtration of blood |
| US4049692A (en) * | 1974-12-16 | 1977-09-20 | Corning Glass Works | Stabilized blood separating composition |
| US4027660A (en) * | 1976-04-02 | 1977-06-07 | Wardlaw Stephen C | Material layer volume determination |
-
1978
- 1978-01-10 DE DE2800934A patent/DE2800934C2/de not_active Expired
- 1978-01-10 IT IT47579/78A patent/IT1103118B/it active
- 1978-01-10 FR FR7800573A patent/FR2377041A1/fr active Granted
- 1978-05-30 US US05/910,807 patent/US4159896A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| IT7847579A0 (it) | 1978-01-10 |
| FR2377041A1 (fr) | 1978-08-04 |
| IT1103118B (it) | 1985-10-14 |
| US4159896A (en) | 1979-07-03 |
| DE2800934A1 (de) | 1978-07-13 |
| FR2377041B1 (cg-RX-API-DMAC7.html) | 1983-09-02 |
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Legal Events
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|---|---|---|---|
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Representative=s name: SCHMITT-NILSON, G., DIPL.-ING. DR.-ING., PAT.-ANW. |
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| 8110 | Request for examination paragraph 44 | ||
| 8127 | New person/name/address of the applicant |
Owner name: WARDLAW, STEPHEN CLARK, BRANFORD, CONN., US LEVINE |
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| 8161 | Application of addition to: |
Ref document number: 2714763 Country of ref document: DE Format of ref document f/p: P |
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| 8128 | New person/name/address of the agent |
Representative=s name: KLUNKER, H., DIPL.-ING. DR.RER.NAT. SCHMITT-NILSON |
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| AF | Is addition to no. |
Ref country code: DE Ref document number: 2714763 Format of ref document f/p: P |
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| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition |