FR2377041A1 - Procede pour obtenir une meilleure separation des couches cellulaires dans un echantillon sanguin centrifuge - Google Patents

Procede pour obtenir une meilleure separation des couches cellulaires dans un echantillon sanguin centrifuge

Info

Publication number
FR2377041A1
FR2377041A1 FR7800573A FR7800573A FR2377041A1 FR 2377041 A1 FR2377041 A1 FR 2377041A1 FR 7800573 A FR7800573 A FR 7800573A FR 7800573 A FR7800573 A FR 7800573A FR 2377041 A1 FR2377041 A1 FR 2377041A1
Authority
FR
France
Prior art keywords
blood sample
cell layer
layer separation
obtaining better
centrifugal blood
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7800573A
Other languages
English (en)
Other versions
FR2377041B1 (fr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of FR2377041A1 publication Critical patent/FR2377041A1/fr
Application granted granted Critical
Publication of FR2377041B1 publication Critical patent/FR2377041B1/fr
Granted legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Ecology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne un procédé permettant de rendre plus nette l'interface entre une couche de granulocytes et la couche d'hématies dans un échantillon centrifugé de sang. On ajoute à l'échantillon sanguin un additif permettant d'augmenter la densité des hématies sans affecter notablement la densité des granulocytes, en particulier l'oxalate de K.
FR7800573A 1977-01-10 1978-01-10 Procede pour obtenir une meilleure separation des couches cellulaires dans un echantillon sanguin centrifuge Granted FR2377041A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US75806577A 1977-01-10 1977-01-10

Publications (2)

Publication Number Publication Date
FR2377041A1 true FR2377041A1 (fr) 1978-08-04
FR2377041B1 FR2377041B1 (fr) 1983-09-02

Family

ID=25050351

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7800573A Granted FR2377041A1 (fr) 1977-01-10 1978-01-10 Procede pour obtenir une meilleure separation des couches cellulaires dans un echantillon sanguin centrifuge

Country Status (4)

Country Link
US (1) US4159896A (fr)
DE (1) DE2800934C2 (fr)
FR (1) FR2377041A1 (fr)
IT (1) IT1103118B (fr)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4310430A (en) * 1979-09-11 1982-01-12 Terumo Corporation α-Olefin-dialkylmaleate-based liquid separating agent
ES8307122A1 (es) * 1981-08-27 1983-06-16 Becton Dickinson Co "perfeccionamientos introducidos en un aparato para introducir reactivos en recipientes de recogida de muestras".
US4594165A (en) * 1984-11-16 1986-06-10 Levine Robert A Method of enhancing separation of abnormally light red cells from granulocytes in a centrifuged blood sample
US4567754A (en) * 1985-03-29 1986-02-04 Wardlaw Stephen C Measurement of small heavy constituent layer in stratified mixture
US5065768A (en) * 1988-09-13 1991-11-19 Safe-Tec Clinical Products, Inc. Self-sealing fluid conduit and collection device
JPH0774772B2 (ja) * 1990-12-31 1995-08-09 エイ． レビン ロバート 血液サンプリング組立体、ターゲット細胞の採取方法およびターゲット成分の採取方法
US5252460A (en) * 1991-10-28 1993-10-12 Fiedler Paul N In vitro detection of ova, parasites, and other formed elements in stool
US5342790A (en) * 1992-10-30 1994-08-30 Becton Dickinson And Company Apparatus for indirect fluorescent assay of blood samples
WO1994029722A1 (fr) * 1993-06-08 1994-12-22 Chronomed, Inc. Appareil et procede de dosage optique en deux phases
US5422018A (en) * 1994-01-31 1995-06-06 Applied Imaging Centrifuge tube and adaptor
US5560830A (en) * 1994-12-13 1996-10-01 Coleman; Charles M. Separator float and tubular body for blood collection and separation and method of use thereof
US5733545A (en) * 1995-03-03 1998-03-31 Quantic Biomedical Partners Platelet glue wound sealant
JPH11508813A (ja) 1995-06-06 1999-08-03 クウォンティック バイオメディカル パートナーズ 血漿を濃縮するための装置及び方法
US5736033A (en) * 1995-12-13 1998-04-07 Coleman; Charles M. Separator float for blood collection tubes with water swellable material
AU4101697A (en) * 1996-11-22 1998-05-28 Robert A. Levine Process for enhancing the aggregation and/or agglutination of erythrocytes prior to centrifugation
US5811303A (en) * 1997-04-01 1998-09-22 Streck Laboratories, Inc. Quantitative buffy coat control composition
US7947236B2 (en) 1999-12-03 2011-05-24 Becton, Dickinson And Company Device for separating components of a fluid sample
US7205157B2 (en) * 2001-01-08 2007-04-17 Becton, Dickinson And Company Method of separating cells from a sample
US7832566B2 (en) 2002-05-24 2010-11-16 Biomet Biologics, Llc Method and apparatus for separating and concentrating a component from a multi-component material including macroparticles
US7992725B2 (en) 2002-05-03 2011-08-09 Biomet Biologics, Llc Buoy suspension fractionation system
US7374678B2 (en) 2002-05-24 2008-05-20 Biomet Biologics, Inc. Apparatus and method for separating and concentrating fluids containing multiple components
US20030205538A1 (en) 2002-05-03 2003-11-06 Randel Dorian Methods and apparatus for isolating platelets from blood
DE10392686T5 (de) 2002-05-24 2005-07-07 Biomet Mfg. Corp., Warsaw Vorrichtung und Verfahren zum Trennen und Konzentrieren von Flüssigkeiten, welche mehrere Komponenten enthalten
US20060278588A1 (en) 2002-05-24 2006-12-14 Woodell-May Jennifer E Apparatus and method for separating and concentrating fluids containing multiple components
US7845499B2 (en) 2002-05-24 2010-12-07 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US7074577B2 (en) 2002-10-03 2006-07-11 Battelle Memorial Institute Buffy coat tube and float system and method
WO2006086201A2 (fr) 2005-02-07 2006-08-17 Hanuman Llc Appareil et procede de concentration de plasma riche en plaquettes
US7866485B2 (en) 2005-02-07 2011-01-11 Hanuman, Llc Apparatus and method for preparing platelet rich plasma and concentrates thereof
JP4961354B2 (ja) 2005-02-07 2012-06-27 ハヌマン リミテッド ライアビリティ カンパニー 多血小板血漿濃縮装置および方法
US8567609B2 (en) 2006-05-25 2013-10-29 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US7806276B2 (en) 2007-04-12 2010-10-05 Hanuman, Llc Buoy suspension fractionation system
US8328024B2 (en) 2007-04-12 2012-12-11 Hanuman, Llc Buoy suspension fractionation system
EP2259774B1 (fr) 2008-02-27 2012-12-12 Biomet Biologics, LLC Procédés et compositions pour administrer un antagoniste de récepteur d interleukine-1antagonist
US8337711B2 (en) 2008-02-29 2012-12-25 Biomet Biologics, Llc System and process for separating a material
US8012077B2 (en) 2008-05-23 2011-09-06 Biomet Biologics, Llc Blood separating device
CN102149473B (zh) 2008-07-21 2014-12-31 贝克顿·迪金森公司 密度相分离装置
ES2452534T3 (es) * 2008-07-21 2014-04-01 Becton, Dickinson And Company Dispositivo de separación de fases de densidad
CA2731156C (fr) 2008-07-21 2013-09-24 Becton, Dickinson And Company Dispositif de separation de phases par densite
US8177072B2 (en) 2008-12-04 2012-05-15 Thermogenesis Corp. Apparatus and method for separating and isolating components of a biological fluid
US8187475B2 (en) 2009-03-06 2012-05-29 Biomet Biologics, Llc Method and apparatus for producing autologous thrombin
US8313954B2 (en) 2009-04-03 2012-11-20 Biomet Biologics, Llc All-in-one means of separating blood components
PL3821980T3 (pl) 2009-05-15 2023-02-20 Becton, Dickinson And Company Urządzenie do rozdzielania faz gęstości
US9011800B2 (en) 2009-07-16 2015-04-21 Biomet Biologics, Llc Method and apparatus for separating biological materials
US8591391B2 (en) 2010-04-12 2013-11-26 Biomet Biologics, Llc Method and apparatus for separating a material
US8377395B2 (en) 2010-04-29 2013-02-19 Charles M. Coleman Integrated blood specimen processor
US9642956B2 (en) 2012-08-27 2017-05-09 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US9895418B2 (en) 2013-03-15 2018-02-20 Biomet Biologics, Llc Treatment of peripheral vascular disease using protein solutions
US10208095B2 (en) 2013-03-15 2019-02-19 Biomet Manufacturing, Llc Methods for making cytokine compositions from tissues using non-centrifugal methods
US10143725B2 (en) 2013-03-15 2018-12-04 Biomet Biologics, Llc Treatment of pain using protein solutions
US20140271589A1 (en) 2013-03-15 2014-09-18 Biomet Biologics, Llc Treatment of collagen defects using protein solutions
US9950035B2 (en) 2013-03-15 2018-04-24 Biomet Biologics, Llc Methods and non-immunogenic compositions for treating inflammatory disorders
GB201306810D0 (en) 2013-04-15 2013-05-29 Cells4Life Group Llp Methods of cell separation
US9694359B2 (en) 2014-11-13 2017-07-04 Becton, Dickinson And Company Mechanical separator for a biological fluid
US9713810B2 (en) 2015-03-30 2017-07-25 Biomet Biologics, Llc Cell washing plunger using centrifugal force
US9757721B2 (en) 2015-05-11 2017-09-12 Biomet Biologics, Llc Cell washing plunger using centrifugal force

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3931018A (en) * 1974-08-09 1976-01-06 Becton, Dickinson And Company Assembly for collection, separation and filtration of blood
FR2295079A1 (fr) * 1974-12-16 1976-07-16 Corning Glass Works Composition stabilisee pour la separation du sang
US4027660A (en) * 1976-04-02 1977-06-07 Wardlaw Stephen C Material layer volume determination

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2102785A (en) * 1935-10-18 1937-12-21 Brooks Clyde Method of and apparatus for sedimentation testing of blood
US2725782A (en) * 1952-08-19 1955-12-06 Worley Carl Milton Apparatus for recording rate of sedimentation in liquids
DE6945462U (de) * 1969-11-17 1970-04-16 Helmut Dr Niepage Pcv-meter
US3684450A (en) * 1970-09-14 1972-08-15 Stanford L Adler Automatic apparatus and method for determining the packed cell volume of whole blood
US3965889A (en) * 1971-11-26 1976-06-29 Commissariat A L'energie Atomique Apparatus for the sampling of blood and the separation of plasma under anaerobic conditions
US3914985A (en) * 1974-03-29 1975-10-28 American Hospital Supply Corp Centrifuging device and method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3931018A (en) * 1974-08-09 1976-01-06 Becton, Dickinson And Company Assembly for collection, separation and filtration of blood
FR2295079A1 (fr) * 1974-12-16 1976-07-16 Corning Glass Works Composition stabilisee pour la separation du sang
US4027660A (en) * 1976-04-02 1977-06-07 Wardlaw Stephen C Material layer volume determination
FR2346692A1 (fr) * 1976-04-02 1977-10-28 Wardlaw Stephen Appareil et procede pour determiner le volume de couches centrifugees

Also Published As

Publication number Publication date
DE2800934A1 (de) 1978-07-13
IT1103118B (it) 1985-10-14
FR2377041B1 (fr) 1983-09-02
US4159896A (en) 1979-07-03
DE2800934C2 (de) 1986-09-18
IT7847579A0 (it) 1978-01-10

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