DE2760244C2 - Verfahren zur Herstellung von α-Glycerophosphat-Oxidase - Google Patents
Verfahren zur Herstellung von α-Glycerophosphat-OxidaseInfo
- Publication number
- DE2760244C2 DE2760244C2 DE19772760244 DE2760244A DE2760244C2 DE 2760244 C2 DE2760244 C2 DE 2760244C2 DE 19772760244 DE19772760244 DE 19772760244 DE 2760244 A DE2760244 A DE 2760244A DE 2760244 C2 DE2760244 C2 DE 2760244C2
- Authority
- DE
- Germany
- Prior art keywords
- medium
- glycerophosphate
- production
- oxidase
- glycerophosphate oxidase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims description 12
- 238000004519 manufacturing process Methods 0.000 title claims description 10
- 101710143182 Alpha-glycerophosphate oxidase Proteins 0.000 title 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 57
- 239000002609 medium Substances 0.000 claims description 23
- 102000004316 Oxidoreductases Human genes 0.000 claims description 19
- 108090000854 Oxidoreductases Proteins 0.000 claims description 19
- 241000194032 Enterococcus faecalis Species 0.000 claims description 12
- 244000005700 microbiome Species 0.000 claims description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 229940041514 candida albicans extract Drugs 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 239000012138 yeast extract Substances 0.000 claims description 6
- 239000012137 tryptone Substances 0.000 claims description 5
- 238000000855 fermentation Methods 0.000 claims description 4
- 230000004151 fermentation Effects 0.000 claims description 4
- 102000000587 Glycerolphosphate Dehydrogenase Human genes 0.000 claims description 2
- 108010041921 Glycerolphosphate Dehydrogenase Proteins 0.000 claims description 2
- 239000001963 growth medium Substances 0.000 claims description 2
- 241001468155 Lactobacillaceae Species 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 235000011187 glycerol Nutrition 0.000 description 20
- 102000004190 Enzymes Human genes 0.000 description 12
- 108090000790 Enzymes Proteins 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 7
- 239000001301 oxygen Substances 0.000 description 7
- 229910052760 oxygen Inorganic materials 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- GNGACRATGGDKBX-UHFFFAOYSA-N dihydroxyacetone phosphate Chemical compound OCC(=O)COP(O)(O)=O GNGACRATGGDKBX-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 230000003647 oxidation Effects 0.000 description 5
- 238000007254 oxidation reaction Methods 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 238000005187 foaming Methods 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- 241000194031 Enterococcus faecium Species 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- AWUCVROLDVIAJX-UHFFFAOYSA-N alpha-glycerophosphate Natural products OCC(O)COP(O)(O)=O AWUCVROLDVIAJX-UHFFFAOYSA-N 0.000 description 2
- 239000002518 antifoaming agent Substances 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 229940076788 pyruvate Drugs 0.000 description 2
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- JRBJSXQPQWSCCF-UHFFFAOYSA-N 3,3'-Dimethoxybenzidine Chemical compound C1=C(N)C(OC)=CC(C=2C=C(OC)C(N)=CC=2)=C1 JRBJSXQPQWSCCF-UHFFFAOYSA-N 0.000 description 1
- UXTIAFYTYOEQHV-UHFFFAOYSA-N 4-(4-amino-3-methoxyphenyl)-2-methoxyaniline;hydron;dichloride Chemical compound [Cl-].[Cl-].C1=C([NH3+])C(OC)=CC(C=2C=C(OC)C([NH3+])=CC=2)=C1 UXTIAFYTYOEQHV-UHFFFAOYSA-N 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 230000009603 aerobic growth Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 239000003708 ampul Substances 0.000 description 1
- 230000009604 anaerobic growth Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- CEYGNZMCCVVXQW-UHFFFAOYSA-N phosphoric acid;propane-1,2-diol Chemical compound CC(O)CO.OP(O)(O)=O CEYGNZMCCVVXQW-UHFFFAOYSA-N 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000008057 potassium phosphate buffer Substances 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- AWUCVROLDVIAJX-GSVOUGTGSA-N sn-glycerol 3-phosphate Chemical compound OC[C@@H](O)COP(O)(O)=O AWUCVROLDVIAJX-GSVOUGTGSA-N 0.000 description 1
- 229940054269 sodium pyruvate Drugs 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US74965076A | 1976-12-10 | 1976-12-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2760244C2 true DE2760244C2 (de) | 1986-03-06 |
Family
ID=25014618
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19772760244 Expired DE2760244C2 (de) | 1976-12-10 | 1977-12-09 | Verfahren zur Herstellung von α-Glycerophosphat-Oxidase |
| DE19772755033 Expired DE2755033C2 (de) | 1976-12-10 | 1977-12-09 | Verfahren zur Herstellung von α-Glycerophosphat-Oxidase |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19772755033 Expired DE2755033C2 (de) | 1976-12-10 | 1977-12-09 | Verfahren zur Herstellung von α-Glycerophosphat-Oxidase |
Country Status (5)
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1095447A (en) * | 1976-12-10 | 1981-02-10 | Prakash S. Masurekar | Process for the production of a-glycero-phosphate oxidase |
| JPS6022915B2 (ja) * | 1978-07-20 | 1985-06-04 | 東洋醸造株式会社 | Ｌ−α−グリセロリン酸オキシダ−ゼの製造法 |
| US4463095A (en) | 1982-11-26 | 1984-07-31 | Toyo Boseki Kabushiki Kaisha | Process for producing α-glycerophosphate oxidase |
| JPS6023835B2 (ja) * | 1983-08-09 | 1985-06-10 | 東洋醸造株式会社 | Ｌ−α−グリセロリン酸オキシダ−ゼ |
| GB2213822B (en) * | 1987-12-15 | 1991-12-18 | Toyo Jozo Kk | Dna having genetic information of l-´-glycerophosphate oxidase and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1095447A (en) * | 1976-12-10 | 1981-02-10 | Prakash S. Masurekar | Process for the production of a-glycero-phosphate oxidase |
| JPS5730470A (en) * | 1980-07-30 | 1982-02-18 | Hitachi Ltd | Automatic switching device of multiple-system color television receiver |
-
1977
- 1977-12-02 CA CA292,229A patent/CA1095447A/en not_active Expired
- 1977-12-08 JP JP14667077A patent/JPS5372892A/ja active Granted
- 1977-12-09 FR FR7737116A patent/FR2392113A1/fr active Granted
- 1977-12-09 DE DE19772760244 patent/DE2760244C2/de not_active Expired
- 1977-12-09 DE DE19772755033 patent/DE2755033C2/de not_active Expired
- 1977-12-12 GB GB5164677A patent/GB1585909A/en not_active Expired
-
1983
- 1983-05-31 JP JP58095097A patent/JPS58216687A/ja active Granted
- 1983-05-31 JP JP9509683A patent/JPS58216686A/ja active Granted
- 1983-05-31 JP JP9509883A patent/JPS58216688A/ja active Granted
Non-Patent Citations (1)
| Title |
|---|
| NICHTS-ERMITTELT * |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS58216688A (ja) | 1983-12-16 |
| FR2392113A1 (fr) | 1978-12-22 |
| JPS62675B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1987-01-08 |
| JPS5372892A (en) | 1978-06-28 |
| JPS5730470B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1982-06-29 |
| DE2755033C2 (de) | 1985-08-22 |
| JPS62676B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1987-01-08 |
| GB1585909A (en) | 1981-03-11 |
| FR2392113B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1980-01-04 |
| CA1095447A (en) | 1981-02-10 |
| JPS58216686A (ja) | 1983-12-16 |
| JPS58216687A (ja) | 1983-12-16 |
| JPS6211598B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1987-03-13 |
| DE2755033A1 (de) | 1978-06-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| Q172 | Divided out of (supplement): |
Ref country code: DE Ref document number: 2755033 |
|
| 8110 | Request for examination paragraph 44 | ||
| AC | Divided out of |
Ref country code: DE Ref document number: 2755033 Format of ref document f/p: P |
|
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: JOHNSON & JOHNSON CLINICAL DIAGNOSTICS, INC., ROCH |
|
| 8328 | Change in the person/name/address of the agent |
Free format text: STREHL, SCHUEBEL-HOPF, GROENING & PARTNER, 80538 MUENCHEN |