DE2713817A1 - Verfahren zum gewinnen von immunglobulin - Google Patents
Verfahren zum gewinnen von immunglobulinInfo
- Publication number
- DE2713817A1 DE2713817A1 DE19772713817 DE2713817A DE2713817A1 DE 2713817 A1 DE2713817 A1 DE 2713817A1 DE 19772713817 DE19772713817 DE 19772713817 DE 2713817 A DE2713817 A DE 2713817A DE 2713817 A1 DE2713817 A1 DE 2713817A1
- Authority
- DE
- Germany
- Prior art keywords
- acid
- precipitation
- immunoglobulin
- carbon atoms
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000034 method Methods 0.000 title claims description 18
- 108060003951 Immunoglobulin Proteins 0.000 title claims description 13
- 102000018358 immunoglobulin Human genes 0.000 title claims description 13
- 239000002253 acid Substances 0.000 claims description 15
- 238000001556 precipitation Methods 0.000 claims description 11
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 claims description 10
- 210000002381 plasma Anatomy 0.000 claims description 10
- 125000004432 carbon atom Chemical group C* 0.000 claims description 7
- 239000005635 Caprylic acid (CAS 124-07-2) Substances 0.000 claims description 5
- 229960002446 octanoic acid Drugs 0.000 claims description 5
- 229920005862 polyol Polymers 0.000 claims description 4
- 150000003077 polyols Chemical class 0.000 claims description 4
- 210000002966 serum Anatomy 0.000 claims description 4
- 229920002560 Polyethylene Glycol 3000 Polymers 0.000 description 7
- 239000002202 Polyethylene glycol Substances 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 108010074605 gamma-Globulins Proteins 0.000 description 6
- 229920001223 polyethylene glycol Polymers 0.000 description 6
- 239000000047 product Substances 0.000 description 5
- 150000007513 acids Chemical class 0.000 description 4
- 238000001990 intravenous administration Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000003171 anti-complementary effect Effects 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 2
- 102000008946 Fibrinogen Human genes 0.000 description 2
- 108010049003 Fibrinogen Proteins 0.000 description 2
- 102000006395 Globulins Human genes 0.000 description 2
- 108010044091 Globulins Proteins 0.000 description 2
- 229920005654 Sephadex Polymers 0.000 description 2
- 239000012507 Sephadex™ Substances 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229940012952 fibrinogen Drugs 0.000 description 2
- 229940072221 immunoglobulins Drugs 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- FBUKVWPVBMHYJY-UHFFFAOYSA-N nonanoic acid Chemical compound CCCCCCCCC(O)=O FBUKVWPVBMHYJY-UHFFFAOYSA-N 0.000 description 2
- 229920001451 polypropylene glycol Polymers 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000002572 Alpha-Globulins Human genes 0.000 description 1
- 108010068307 Alpha-Globulins Proteins 0.000 description 1
- 102000006734 Beta-Globulins Human genes 0.000 description 1
- 108010087504 Beta-Globulins Proteins 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- GOOHAUXETOMSMM-UHFFFAOYSA-N Propylene oxide Chemical compound CC1CO1 GOOHAUXETOMSMM-UHFFFAOYSA-N 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229920001400 block copolymer Polymers 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 150000002009 diols Chemical class 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000000760 immunoelectrophoresis Methods 0.000 description 1
- 229940027941 immunoglobulin g Drugs 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000570 polyether Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- -1 polyoxypropylene Polymers 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000011146 sterile filtration Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK162876AA DK139056B (da) | 1976-04-06 | 1976-04-06 | Fremgangsmåde til udvinding af immunoglobulin, der er egnet til intravenøs indgivelse. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2713817A1 true DE2713817A1 (de) | 1977-10-27 |
Family
ID=8106680
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19772713817 Withdrawn DE2713817A1 (de) | 1976-04-06 | 1977-03-29 | Verfahren zum gewinnen von immunglobulin |
Country Status (20)
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5625114A (en) * | 1979-08-07 | 1981-03-10 | Green Cross Corp:The | Preparation of human gamma globulin |
| US4590002A (en) * | 1984-12-10 | 1986-05-20 | Ortho Diagnostic Systems, Inc. | Methods for preparation of highly purified, gamma globulins free of hepatitis-B-virus infectivity |
| FR2578425B1 (fr) * | 1985-03-07 | 1988-06-10 | Nal Transfusion Sanguine Centr | Preparations d'immunoglobulines presentant des titres eleves en anticorps bloquants anti-allergenes, leur preparation et leurs applications pour le traitement d'allergies |
| FR2632308B1 (fr) * | 1988-06-07 | 1991-08-16 | Fondation Nale Transfusion San | Procede et installation de fractionnement en continu de proteines vegetales animales ou humaines |
| CA1308023C (en) * | 1988-07-29 | 1992-09-29 | William Austin James Mcauley | Immunoglobulin extraction utilizing properties of colloidal solutions |
| US5525519A (en) * | 1992-01-07 | 1996-06-11 | Middlesex Sciences, Inc. | Method for isolating biomolecules from a biological sample with linear polymers |
| US5367054A (en) * | 1993-04-12 | 1994-11-22 | Stolle Research & Development Corp. | Large-scale purification of egg immunoglobulin |
| WO2005113604A2 (en) * | 2004-05-14 | 2005-12-01 | Hematech, Llc | Methods for immunoglobulin purification |
| US20060226086A1 (en) * | 2005-04-08 | 2006-10-12 | Robinson Thomas C | Centrifuge for blood processing systems |
| TWI442965B (zh) * | 2006-11-01 | 2014-07-01 | Biogen Idec Inc | 使用低ｐＨ及二價陽離子分離生物巨分子的方法 |
| CN102065870B (zh) * | 2008-04-16 | 2014-11-19 | 比奥根艾迪克Ma公司 | 使用聚亚烷基二醇和过渡金属分离生物大分子的方法 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3100737A (en) * | 1958-02-05 | 1963-08-13 | Auerswald Wilhelm | Method of preparing a plasma protein solution free of active hepatitis virus and product produced thereby |
| NL286954A (enrdf_load_stackoverflow) * | 1962-01-03 | |||
| NL6702923A (enrdf_load_stackoverflow) * | 1966-04-06 | 1967-10-09 | ||
| US3916026A (en) * | 1968-09-19 | 1975-10-28 | Biotest Serum Institut Gmbh | Method for the preparation of gamma-globulin suitable for intravenous use |
| US3869436A (en) * | 1971-06-01 | 1975-03-04 | Statens Bakteriologiska Lab | Method for fractionating plasma proteins using peg and ion-exchangers |
| US3770631A (en) * | 1971-06-29 | 1973-11-06 | Baxter Laboratories Inc | Clarification of blood serum and plasma |
| JPS5620287B2 (enrdf_load_stackoverflow) * | 1972-06-19 | 1981-05-13 | ||
| US3880989A (en) * | 1973-01-30 | 1975-04-29 | Baxter Laboratories Inc | Production of antisera comprising fractionating plasma or serum with an ethylene oxide-polyoxypropylene block copolymer |
| US3808189A (en) * | 1973-03-15 | 1974-04-30 | American Cyanamid Co | Isolation of gamma globulin preparations enriched in iga and igm using polyethylene glycol |
| JPS5417002B2 (enrdf_load_stackoverflow) * | 1974-02-18 | 1979-06-27 | ||
| CA1064396A (en) * | 1975-02-18 | 1979-10-16 | Myer L. Coval | Fractional precipitation of gamma globulin with polyethylene glycol |
| DE2527064C3 (de) * | 1975-06-18 | 1979-11-15 | Biotest-Serum-Institut Gmbh, 6000 Frankfurt | Verfahren zur Herstellung eines intravenösen Nativ-Human-Immunglobulin-Präparates mit natürlicher Halbwertszeit und gegenüber dem Ausgangsmaterial unveränderten Antikörperaktivität |
| US4021540A (en) * | 1975-07-28 | 1977-05-03 | Ortho Diagnostics Inc. | Preparation of a hepatitis B immune globulin and use thereof as a prophylactic material |
| FR2322611A2 (fr) * | 1975-09-06 | 1977-04-01 | Biotest Serum Institut Gmbh | Procede de preparation de gamma-globuline |
| US4075193A (en) * | 1976-11-26 | 1978-02-21 | Parke, Davis & Company | Process for producing intravenous immune globulin |
| US4124576A (en) * | 1976-12-03 | 1978-11-07 | Coval M L | Method of producing intravenously injectable gamma globulin |
-
1976
- 1976-04-06 DK DK162876AA patent/DK139056B/da not_active IP Right Cessation
-
1977
- 1977-03-29 DE DE19772713817 patent/DE2713817A1/de not_active Withdrawn
- 1977-03-29 BE BE176213A patent/BE852995A/xx not_active IP Right Cessation
- 1977-03-30 IL IL51782A patent/IL51782A/xx unknown
- 1977-03-31 AR AR267073A patent/AR211890A1/es active
- 1977-03-31 GB GB13575/77A patent/GB1551865A/en not_active Expired
- 1977-03-31 IT IT21982/77A patent/IT1075148B/it active
- 1977-04-01 HU HU77NO212A patent/HU175511B/hu unknown
- 1977-04-01 NL NL7703572A patent/NL7703572A/xx not_active Application Discontinuation
- 1977-04-01 SE SE7703843A patent/SE7703843L/ not_active Application Discontinuation
- 1977-04-02 ES ES457482A patent/ES457482A1/es not_active Expired
- 1977-04-04 AU AU23910/77A patent/AU511474B2/en not_active Expired
- 1977-04-04 DD DD7700198240A patent/DD129451A5/xx unknown
- 1977-04-05 CA CA275,585A patent/CA1072444A/en not_active Expired
- 1977-04-05 FI FI771071A patent/FI61191C/fi not_active IP Right Cessation
- 1977-04-05 NO NO771225A patent/NO146763C/no unknown
- 1977-04-05 FR FR7710326A patent/FR2347379A1/fr active Granted
- 1977-04-05 CH CH428777A patent/CH628813A5/de not_active IP Right Cessation
- 1977-04-06 JP JP3858877A patent/JPS52122620A/ja active Pending
-
1981
- 1981-08-13 US US06/292,386 patent/USRE31268E/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DK139056C (enrdf_load_stackoverflow) | 1979-05-21 |
| FI61191B (fi) | 1982-02-26 |
| CA1072444A (en) | 1980-02-26 |
| FR2347379B1 (enrdf_load_stackoverflow) | 1981-11-20 |
| GB1551865A (en) | 1979-09-05 |
| FR2347379A1 (fr) | 1977-11-04 |
| IT1075148B (it) | 1985-04-22 |
| DD129451A5 (de) | 1978-01-18 |
| NO146763C (no) | 1982-12-08 |
| DK162876A (enrdf_load_stackoverflow) | 1977-10-07 |
| NL7703572A (nl) | 1977-10-10 |
| IL51782A0 (en) | 1977-05-31 |
| CH628813A5 (en) | 1982-03-31 |
| FI771071A7 (enrdf_load_stackoverflow) | 1977-10-07 |
| HU175511B (hu) | 1980-08-28 |
| SE7703843L (sv) | 1977-10-07 |
| AU2391077A (en) | 1978-10-12 |
| JPS52122620A (en) | 1977-10-15 |
| FI61191C (fi) | 1982-06-10 |
| AR211890A1 (es) | 1978-03-31 |
| ES457482A1 (es) | 1978-03-16 |
| IL51782A (en) | 1980-12-31 |
| NO771225L (no) | 1977-10-07 |
| AU511474B2 (en) | 1980-08-21 |
| DK139056B (da) | 1978-12-11 |
| NO146763B (no) | 1982-08-30 |
| BE852995A (fr) | 1977-07-18 |
| USRE31268E (en) | 1983-06-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8141 | Disposal/no request for examination |