DE2513848A1 - Verfahren zur gewinnung ununterbrochener staemme von tumorzellen in vitro - Google Patents
Verfahren zur gewinnung ununterbrochener staemme von tumorzellen in vitroInfo
- Publication number
- DE2513848A1 DE2513848A1 DE19752513848 DE2513848A DE2513848A1 DE 2513848 A1 DE2513848 A1 DE 2513848A1 DE 19752513848 DE19752513848 DE 19752513848 DE 2513848 A DE2513848 A DE 2513848A DE 2513848 A1 DE2513848 A1 DE 2513848A1
- Authority
- DE
- Germany
- Prior art keywords
- vitro
- tumor cells
- strains
- cells
- tumor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000004881 tumor cell Anatomy 0.000 title claims description 45
- 238000000338 in vitro Methods 0.000 title claims description 27
- 238000000034 method Methods 0.000 title claims description 24
- 210000004027 cell Anatomy 0.000 claims description 24
- 210000003293 antilymphocyte serum Anatomy 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 9
- 230000000295 complement effect Effects 0.000 claims description 8
- 231100000409 cytocidal Toxicity 0.000 claims description 7
- 230000000445 cytocidal effect Effects 0.000 claims description 7
- 238000004113 cell culture Methods 0.000 claims description 6
- 210000001616 monocyte Anatomy 0.000 claims description 6
- 238000011477 surgical intervention Methods 0.000 claims description 6
- 210000002540 macrophage Anatomy 0.000 claims description 5
- 201000011510 cancer Diseases 0.000 claims description 4
- 239000002085 irritant Substances 0.000 claims 1
- 231100000021 irritant Toxicity 0.000 claims 1
- 210000004698 lymphocyte Anatomy 0.000 description 15
- 201000009030 Carcinoma Diseases 0.000 description 9
- 210000002966 serum Anatomy 0.000 description 8
- 206010028980 Neoplasm Diseases 0.000 description 7
- 230000003013 cytotoxicity Effects 0.000 description 7
- 231100000135 cytotoxicity Toxicity 0.000 description 7
- 239000000427 antigen Substances 0.000 description 6
- 102000036639 antigens Human genes 0.000 description 6
- 108091007433 antigens Proteins 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 241000283073 Equus caballus Species 0.000 description 4
- 206010027476 Metastases Diseases 0.000 description 4
- 210000004556 brain Anatomy 0.000 description 4
- 208000005017 glioblastoma Diseases 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 208000003174 Brain Neoplasms Diseases 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- 210000000038 chest Anatomy 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 230000009401 metastasis Effects 0.000 description 3
- 206010003571 Astrocytoma Diseases 0.000 description 2
- 102000004142 Trypsin Human genes 0.000 description 2
- 108090000631 Trypsin Proteins 0.000 description 2
- 230000000781 anti-lymphocytic effect Effects 0.000 description 2
- 244000309466 calf Species 0.000 description 2
- 230000036952 cancer formation Effects 0.000 description 2
- 230000003157 cancerolytic effect Effects 0.000 description 2
- 230000003327 cancerostatic effect Effects 0.000 description 2
- 231100000433 cytotoxic Toxicity 0.000 description 2
- 230000001472 cytotoxic effect Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 210000001685 thyroid gland Anatomy 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 206010013457 Dissociation Diseases 0.000 description 1
- 241000446313 Lamella Species 0.000 description 1
- 206010059282 Metastases to central nervous system Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 208000033781 Thyroid carcinoma Diseases 0.000 description 1
- 208000024770 Thyroid neoplasm Diseases 0.000 description 1
- 201000005179 adrenal carcinoma Diseases 0.000 description 1
- 230000001919 adrenal effect Effects 0.000 description 1
- 210000004100 adrenal gland Anatomy 0.000 description 1
- 230000000735 allogeneic effect Effects 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 230000005809 anti-tumor immunity Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 244000221110 common millet Species 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 208000018459 dissociative disease Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000003328 fibroblastic effect Effects 0.000 description 1
- 230000000043 immunodepressive effect Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 231100001083 no cytotoxicity Toxicity 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000036647 reaction Effects 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 201000002510 thyroid cancer Diseases 0.000 description 1
- 208000013077 thyroid gland carcinoma Diseases 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Oncology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7410458A FR2265861B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1974-03-27 | 1974-03-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2513848A1 true DE2513848A1 (de) | 1975-10-09 |
Family
ID=9136867
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19752513848 Pending DE2513848A1 (de) | 1974-03-27 | 1975-03-27 | Verfahren zur gewinnung ununterbrochener staemme von tumorzellen in vitro |
Country Status (8)
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3950851A (en) * | 1975-03-05 | 1976-04-20 | Bergersen Earl Olaf | Orthodontic positioner and method for improving retention of tooth alignment therewith |
| US4135975A (en) * | 1977-12-14 | 1979-01-23 | Lichtman Marshall A | Obtaining human cell lines that elaborate colony stimulating activity for marrow cells of man and other species and methods of preparing same |
| US4228236A (en) * | 1978-05-15 | 1980-10-14 | Northwestern University | Process of producing carcinoembryonic antigen |
| DE2836362A1 (de) * | 1978-08-19 | 1980-03-06 | Behringwerke Ag | Diagnostisches mittel |
| US6833270B2 (en) * | 2001-11-27 | 2004-12-21 | Biorep Technologies, Inc. | Apparatus and method for isolating cells from organs |
| US20040248077A1 (en) * | 2002-11-27 | 2004-12-09 | Horacio L. Rodriguez Rilo | Cell separation apparatus |
| US20040161845A1 (en) * | 2003-02-14 | 2004-08-19 | Poo Ramon E. | Heating apparatus |
-
1974
- 1974-03-27 FR FR7410458A patent/FR2265861B2/fr not_active Expired
-
1975
- 1975-03-18 GB GB1112175A patent/GB1474006A/en not_active Expired
- 1975-03-20 BE BE1006538A patent/BE826886A/xx unknown
- 1975-03-20 US US05/560,241 patent/US4017361A/en not_active Expired - Lifetime
- 1975-03-20 CH CH360475A patent/CH606438A5/xx not_active IP Right Cessation
- 1975-03-25 NL NL7503607A patent/NL7503607A/xx not_active Application Discontinuation
- 1975-03-27 JP JP50037708A patent/JPS50129786A/ja active Pending
- 1975-03-27 DE DE19752513848 patent/DE2513848A1/de active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| FR2265861A2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1975-10-24 |
| BE826886A (fr) | 1975-09-22 |
| FR2265861B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1978-08-04 |
| US4017361A (en) | 1977-04-12 |
| NL7503607A (nl) | 1975-09-30 |
| GB1474006A (en) | 1977-05-18 |
| JPS50129786A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1975-10-14 |
| CH606438A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1978-10-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OHJ | Non-payment of the annual fee |