DE2112727A1 - Erhaltung der neutralen Proteaseaktivitaet in alkalische proteaseenthaltenden Enzymgemischen unter Verwendung natuerlicher competitiver Proteinsubstrate - Google Patents
Erhaltung der neutralen Proteaseaktivitaet in alkalische proteaseenthaltenden Enzymgemischen unter Verwendung natuerlicher competitiver ProteinsubstrateInfo
- Publication number
- DE2112727A1 DE2112727A1 DE19712112727 DE2112727A DE2112727A1 DE 2112727 A1 DE2112727 A1 DE 2112727A1 DE 19712112727 DE19712112727 DE 19712112727 DE 2112727 A DE2112727 A DE 2112727A DE 2112727 A1 DE2112727 A1 DE 2112727A1
- Authority
- DE
- Germany
- Prior art keywords
- mixture
- neutral
- activity
- solution
- protease activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000000694 effects Effects 0.000 title claims description 42
- 239000000203 mixture Substances 0.000 title claims description 40
- 102000004190 Enzymes Human genes 0.000 title claims description 33
- 108090000790 Enzymes Proteins 0.000 title claims description 33
- 108091005658 Basic proteases Proteins 0.000 title claims description 28
- 108090000145 Bacillolysin Proteins 0.000 title claims description 25
- 108091005507 Neutral proteases Proteins 0.000 title claims description 25
- 102000035092 Neutral proteases Human genes 0.000 title claims description 22
- 230000002860 competitive effect Effects 0.000 title claims description 15
- 239000000758 substrate Substances 0.000 title claims description 14
- 102000004169 proteins and genes Human genes 0.000 title claims description 13
- 108090000623 proteins and genes Proteins 0.000 title claims description 13
- 238000004321 preservation Methods 0.000 title description 2
- 239000000243 solution Substances 0.000 claims description 46
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 235000018102 proteins Nutrition 0.000 claims description 12
- 239000005018 casein Substances 0.000 claims description 11
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 11
- 235000021240 caseins Nutrition 0.000 claims description 11
- 235000013339 cereals Nutrition 0.000 claims description 9
- 230000007935 neutral effect Effects 0.000 claims description 9
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 8
- 235000005822 corn Nutrition 0.000 claims description 8
- 235000012054 meals Nutrition 0.000 claims description 8
- 235000013372 meat Nutrition 0.000 claims description 8
- 235000019733 Fish meal Nutrition 0.000 claims description 7
- 239000004467 fishmeal Substances 0.000 claims description 7
- 239000003755 preservative agent Substances 0.000 claims description 6
- 108010068370 Glutens Proteins 0.000 claims description 5
- 235000021312 gluten Nutrition 0.000 claims description 5
- 230000002335 preservative effect Effects 0.000 claims description 5
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims description 4
- 229910001424 calcium ion Inorganic materials 0.000 claims description 4
- 239000012895 dilution Substances 0.000 claims description 2
- 238000010790 dilution Methods 0.000 claims description 2
- 230000002441 reversible effect Effects 0.000 claims description 2
- 241000209149 Zea Species 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 239000006072 paste Substances 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 description 31
- 102000035195 Peptidases Human genes 0.000 description 9
- 108091005804 Peptidases Proteins 0.000 description 9
- 239000007983 Tris buffer Substances 0.000 description 8
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 8
- 239000004365 Protease Substances 0.000 description 7
- 230000006641 stabilisation Effects 0.000 description 7
- 238000011105 stabilization Methods 0.000 description 7
- 240000008042 Zea mays Species 0.000 description 6
- 239000007864 aqueous solution Substances 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000017854 proteolysis Effects 0.000 description 2
- 230000002797 proteolythic effect Effects 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 102000005606 Activins Human genes 0.000 description 1
- 108010059616 Activins Proteins 0.000 description 1
- 208000035404 Autolysis Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 206010057248 Cell death Diseases 0.000 description 1
- CJUMAFVKTCBCJK-UHFFFAOYSA-N N-benzyloxycarbonylglycine Chemical compound OC(=O)CNC(=O)OCC1=CC=CC=C1 CJUMAFVKTCBCJK-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000000488 activin Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- -1 benzyloxycarbonylglycine para-nitrophenyl ester Chemical class 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 150000001669 calcium Chemical class 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 description 1
- 239000001639 calcium acetate Substances 0.000 description 1
- 229960005147 calcium acetate Drugs 0.000 description 1
- 235000011092 calcium acetate Nutrition 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000019688 fish Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000005022 packaging material Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 230000004952 protein activity Effects 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000028043 self proteolysis Effects 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2086870A | 1970-03-18 | 1970-03-18 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2112727A1 true DE2112727A1 (de) | 1971-10-07 |
Family
ID=21801025
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19712112727 Pending DE2112727A1 (de) | 1970-03-18 | 1971-03-17 | Erhaltung der neutralen Proteaseaktivitaet in alkalische proteaseenthaltenden Enzymgemischen unter Verwendung natuerlicher competitiver Proteinsubstrate |
Country Status (5)
| Country | Link |
|---|---|
| BE (1) | BE764371A (enrdf_load_stackoverflow) |
| DE (1) | DE2112727A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2084752A5 (enrdf_load_stackoverflow) |
| IT (1) | IT951005B (enrdf_load_stackoverflow) |
| NL (1) | NL7103490A (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2825624B1 (en) | 2012-03-16 | 2018-04-25 | Monosol, LLC | Water soluble compositions incorporating enzymes, and method of making same |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2537991A1 (fr) * | 1982-12-20 | 1984-06-22 | Sanders | Procede pour stabiliser des enzymes liquides, enzymes liquides ainsi stabilisees et aliment renfermant de telles enzymes notamment pour des animaux monogastriques |
-
1971
- 1971-03-16 NL NL7103490A patent/NL7103490A/xx unknown
- 1971-03-17 FR FR7109344A patent/FR2084752A5/fr not_active Expired
- 1971-03-17 BE BE764371A patent/BE764371A/xx unknown
- 1971-03-17 IT IT21904/71A patent/IT951005B/it active
- 1971-03-17 DE DE19712112727 patent/DE2112727A1/de active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2825624B1 (en) | 2012-03-16 | 2018-04-25 | Monosol, LLC | Water soluble compositions incorporating enzymes, and method of making same |
Also Published As
| Publication number | Publication date |
|---|---|
| NL7103490A (enrdf_load_stackoverflow) | 1971-09-21 |
| FR2084752A5 (en) | 1971-12-17 |
| BE764371A (fr) | 1971-09-17 |
| IT951005B (it) | 1973-06-30 |
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