DE1768934A1 - Verfahren zur Anreicherung von Polypeptiden,vorzugsweise von Enzymen und Enzym-Inhibitoren - Google Patents
Verfahren zur Anreicherung von Polypeptiden,vorzugsweise von Enzymen und Enzym-InhibitorenInfo
- Publication number
- DE1768934A1 DE1768934A1 DE19681768934 DE1768934A DE1768934A1 DE 1768934 A1 DE1768934 A1 DE 1768934A1 DE 19681768934 DE19681768934 DE 19681768934 DE 1768934 A DE1768934 A DE 1768934A DE 1768934 A1 DE1768934 A1 DE 1768934A1
- Authority
- DE
- Germany
- Prior art keywords
- inhibitor
- enzyme
- resin
- complex
- trypsin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000004190 Enzymes Human genes 0.000 title claims description 34
- 108090000790 Enzymes Proteins 0.000 title claims description 34
- 238000000034 method Methods 0.000 title claims description 19
- 239000002532 enzyme inhibitor Substances 0.000 title claims description 14
- 108090000765 processed proteins & peptides Proteins 0.000 title claims description 12
- 229920001184 polypeptide Polymers 0.000 title claims description 11
- 102000004196 processed proteins & peptides Human genes 0.000 title claims description 11
- 239000003112 inhibitor Substances 0.000 claims description 50
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000010494 dissociation reaction Methods 0.000 claims description 8
- 230000005593 dissociations Effects 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 7
- 229940125532 enzyme inhibitor Drugs 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 2
- 239000011347 resin Substances 0.000 description 71
- 229920005989 resin Polymers 0.000 description 71
- 229940088598 enzyme Drugs 0.000 description 29
- 239000012588 trypsin Substances 0.000 description 29
- 108090000631 Trypsin Proteins 0.000 description 20
- 102000004142 Trypsin Human genes 0.000 description 20
- 239000000243 solution Substances 0.000 description 18
- 108060005987 Kallikrein Proteins 0.000 description 11
- 102000001399 Kallikrein Human genes 0.000 description 11
- 102000004169 proteins and genes Human genes 0.000 description 9
- 108090000623 proteins and genes Proteins 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 239000006228 supernatant Substances 0.000 description 8
- 239000000872 buffer Substances 0.000 description 7
- 210000000496 pancreas Anatomy 0.000 description 7
- 238000003756 stirring Methods 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 6
- 230000007935 neutral effect Effects 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 229940122920 Kallikrein inhibitor Drugs 0.000 description 5
- OHJMTUPIZMNBFR-UHFFFAOYSA-N biuret Chemical compound NC(=O)NC(N)=O OHJMTUPIZMNBFR-UHFFFAOYSA-N 0.000 description 5
- 239000007853 buffer solution Substances 0.000 description 5
- 239000011541 reaction mixture Substances 0.000 description 5
- 244000068988 Glycine max Species 0.000 description 4
- 235000010469 Glycine max Nutrition 0.000 description 4
- 101000975003 Homo sapiens Kallistatin Proteins 0.000 description 4
- 101001077723 Homo sapiens Serine protease inhibitor Kazal-type 6 Proteins 0.000 description 4
- 102100023012 Kallistatin Human genes 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- NAQMVNRVTILPCV-UHFFFAOYSA-N hexane-1,6-diamine Chemical compound NCCCCCCN NAQMVNRVTILPCV-UHFFFAOYSA-N 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 239000002753 trypsin inhibitor Substances 0.000 description 4
- 108010039627 Aprotinin Proteins 0.000 description 3
- 229920005654 Sephadex Polymers 0.000 description 3
- 229940122618 Trypsin inhibitor Drugs 0.000 description 3
- 101710162629 Trypsin inhibitor Proteins 0.000 description 3
- 230000002378 acidificating effect Effects 0.000 description 3
- 125000000129 anionic group Chemical group 0.000 description 3
- 150000002357 guanidines Chemical class 0.000 description 3
- 239000012266 salt solution Substances 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 229940108519 trasylol Drugs 0.000 description 3
- ICSNLGPSRYBMBD-UHFFFAOYSA-N 2-aminopyridine Chemical compound NC1=CC=CC=N1 ICSNLGPSRYBMBD-UHFFFAOYSA-N 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- 108090000317 Chymotrypsin Proteins 0.000 description 2
- 239000012507 Sephadex™ Substances 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
- 229960002376 chymotrypsin Drugs 0.000 description 2
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 239000010414 supernatant solution Substances 0.000 description 2
- APJYDQYYACXCRM-UHFFFAOYSA-N tryptamine Chemical compound C1=CC=C2C(CCN)=CNC2=C1 APJYDQYYACXCRM-UHFFFAOYSA-N 0.000 description 2
- NAHSJIQXYWGMJN-UHFFFAOYSA-N 1-cyclohexylpropane-1,3-diamine Chemical compound NCCC(N)C1CCCCC1 NAHSJIQXYWGMJN-UHFFFAOYSA-N 0.000 description 1
- NGNBDVOYPDDBFK-UHFFFAOYSA-N 2-[2,4-di(pentan-2-yl)phenoxy]acetyl chloride Chemical group CCCC(C)C1=CC=C(OCC(Cl)=O)C(C(C)CCC)=C1 NGNBDVOYPDDBFK-UHFFFAOYSA-N 0.000 description 1
- UVLSCMIEPPWCHZ-UHFFFAOYSA-N 3-piperazin-1-ylpropan-1-amine Chemical compound NCCCN1CCNCC1 UVLSCMIEPPWCHZ-UHFFFAOYSA-N 0.000 description 1
- NUKYPUAOHBNCPY-UHFFFAOYSA-N 4-aminopyridine Chemical compound NC1=CC=NC=C1 NUKYPUAOHBNCPY-UHFFFAOYSA-N 0.000 description 1
- KNIUHBNRWZGIQQ-UHFFFAOYSA-N 7-diethoxyphosphinothioyloxy-4-methylchromen-2-one Chemical compound CC1=CC(=O)OC2=CC(OP(=S)(OCC)OCC)=CC=C21 KNIUHBNRWZGIQQ-UHFFFAOYSA-N 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000003914 Cholinesterases Human genes 0.000 description 1
- 108090000322 Cholinesterases Proteins 0.000 description 1
- 229940122644 Chymotrypsin inhibitor Drugs 0.000 description 1
- 101710185564 Chymotrypsin-1 Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108010088842 Fibrinolysin Proteins 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010003272 Hyaluronate lyase Proteins 0.000 description 1
- 102000001974 Hyaluronidases Human genes 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 101710141454 Nucleoprotein Proteins 0.000 description 1
- 108010067372 Pancreatic elastase Proteins 0.000 description 1
- 102000016387 Pancreatic elastase Human genes 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 108010087702 Penicillinase Proteins 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 108090000783 Renin Proteins 0.000 description 1
- 102100028255 Renin Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 108090000190 Thrombin Proteins 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 239000002156 adsorbate Substances 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 150000008064 anhydrides Chemical group 0.000 description 1
- HOPRXXXSABQWAV-UHFFFAOYSA-N anhydrous collidine Natural products CC1=CC=NC(C)=C1C HOPRXXXSABQWAV-UHFFFAOYSA-N 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- IVRMZWNICZWHMI-UHFFFAOYSA-N azide group Chemical group [N-]=[N+]=[N-] IVRMZWNICZWHMI-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- HQABUPZFAYXKJW-UHFFFAOYSA-N butan-1-amine Chemical compound CCCCN HQABUPZFAYXKJW-UHFFFAOYSA-N 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000003541 chymotrypsin inhibitor Substances 0.000 description 1
- UTBIMNXEDGNJFE-UHFFFAOYSA-N collidine Natural products CC1=CC=C(C)C(C)=N1 UTBIMNXEDGNJFE-UHFFFAOYSA-N 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 150000004985 diamines Chemical class 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000004049 embossing Methods 0.000 description 1
- 229960004979 fampridine Drugs 0.000 description 1
- 125000001153 fluoro group Chemical group F* 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 229960002773 hyaluronidase Drugs 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- IQPQWNKOIGAROB-UHFFFAOYSA-N isocyanate group Chemical group [N-]=C=O IQPQWNKOIGAROB-UHFFFAOYSA-N 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004001 molecular interaction Effects 0.000 description 1
- DILRJUIACXKSQE-UHFFFAOYSA-N n',n'-dimethylethane-1,2-diamine Chemical compound CN(C)CCN DILRJUIACXKSQE-UHFFFAOYSA-N 0.000 description 1
- GFMUAWWLXMJVRV-UHFFFAOYSA-N n',n'-dimethylethane-1,2-diamine;hydrochloride Chemical compound [Cl-].C[NH+](C)CCN GFMUAWWLXMJVRV-UHFFFAOYSA-N 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 229950009506 penicillinase Drugs 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 229940012957 plasmin Drugs 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 210000001625 seminal vesicle Anatomy 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000007974 sodium acetate buffer Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- GFYHSKONPJXCDE-UHFFFAOYSA-N sym-collidine Natural products CC1=CN=C(C)C(C)=C1 GFYHSKONPJXCDE-UHFFFAOYSA-N 0.000 description 1
- 229960004072 thrombin Drugs 0.000 description 1
- 201000010653 vesiculitis Diseases 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6445—Kallikreins (3.4.21.34; 3.4.21.35)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (22)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19681768934 DE1768934A1 (de) | 1968-07-15 | 1968-07-15 | Verfahren zur Anreicherung von Polypeptiden,vorzugsweise von Enzymen und Enzym-Inhibitoren |
| CH964469A CH512522A (de) | 1968-07-15 | 1969-06-24 | Verfahren zur Anreicherung von Proteinen |
| IL6932511A IL32511A (en) | 1968-07-15 | 1969-06-30 | Enrichment of trypsin,chymotrypsin or kallikrein or of their inhibitors |
| CA055716A CA923834A (en) | 1968-07-15 | 1969-06-30 | Enrichment of polypeptides |
| GB3335669A GB1228698A (enrdf_load_stackoverflow) | 1968-07-15 | 1969-07-02 | |
| AT643769A AT287188B (de) | 1968-07-15 | 1969-07-04 | Verfahren zur Anreicherung von Polypeptiden, vorzugsweise von Enzymen und Enzym-Inhibitoren |
| IE918/69A IE33418B1 (en) | 1968-07-15 | 1969-07-04 | Biochemical enrichment process |
| RO6900060439A RO61383A (fr) | 1968-07-15 | 1969-07-05 | Procede d'enrichissement des polypeptides,en particulierdes enzymes et inhibiteurs d'enzymes |
| LU59055A LU59055A1 (enrdf_load_stackoverflow) | 1968-07-15 | 1969-07-08 | |
| NO2941/69A NO124538B (enrdf_load_stackoverflow) | 1968-07-15 | 1969-07-14 | |
| ES369475A ES369475A1 (es) | 1968-07-15 | 1969-07-14 | Procedimiento para el enriquecimiento de polipeptidos. |
| DK380869AA DK122433B (da) | 1968-07-15 | 1969-07-14 | Fremgangsmåde til koncentrering, rensning og/eller isolering af enzymer eller deres inhibitorer. |
| PL1969134799A PL82620B1 (enrdf_load_stackoverflow) | 1968-07-15 | 1969-07-14 | |
| CS4976A CS150676B2 (enrdf_load_stackoverflow) | 1968-07-15 | 1969-07-14 | |
| JP44055134A JPS5212242B1 (enrdf_load_stackoverflow) | 1968-07-15 | 1969-07-14 | |
| SE6909961A SE372276B (enrdf_load_stackoverflow) | 1968-07-15 | 1969-07-14 | |
| BE736069A BE736069A (enrdf_load_stackoverflow) | 1968-07-15 | 1969-07-14 | |
| EG381/69*A EG10148A (en) | 1968-07-15 | 1969-07-15 | Process for enrichment of polypeptides |
| FI692106A FI47375C (fi) | 1968-07-15 | 1969-07-15 | Tapa rikastaa trypsiiniä, kymotrypsiiniä ja kallikreiinia sekä niiden inhibiittoreita. |
| NL6910881A NL6910881A (enrdf_load_stackoverflow) | 1968-07-15 | 1969-07-15 | |
| FR6924062A FR2014613A1 (enrdf_load_stackoverflow) | 1968-07-15 | 1969-07-15 | |
| US00229198A US3834990A (en) | 1968-07-15 | 1972-02-24 | Isolation of enzymes and enzyme inhibitors |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19681768934 DE1768934A1 (de) | 1968-07-15 | 1968-07-15 | Verfahren zur Anreicherung von Polypeptiden,vorzugsweise von Enzymen und Enzym-Inhibitoren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE1768934A1 true DE1768934A1 (de) | 1972-03-16 |
Family
ID=5699923
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19681768934 Pending DE1768934A1 (de) | 1968-07-15 | 1968-07-15 | Verfahren zur Anreicherung von Polypeptiden,vorzugsweise von Enzymen und Enzym-Inhibitoren |
Country Status (21)
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3196315A1 (en) | 2016-01-19 | 2017-07-26 | ORITEST spol. s r.o. | Spherical pellets, manufacturing process of such pellets, use, and a detection tube comprising such pellets |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4054646A (en) | 1973-07-30 | 1977-10-18 | General Electric | Method and apparatus for detection of antibodies and antigens |
| FR2802817B1 (fr) * | 1999-12-23 | 2002-10-11 | Centre Nat Rech Scient | Nouveaux inhibiteurs de glycosidases et leurs applications pharmacologiques, notamment pour traiter le diabete |
| AU2007272494B2 (en) | 2006-07-14 | 2011-07-28 | Wisconsin Alumni Research Foundation | Adsorptive membranes for trapping viruses |
| CN117099869B (zh) * | 2023-06-27 | 2024-11-15 | 河南工业大学 | 一种小麦蛋白肽螯合钙的制备方法 |
-
1968
- 1968-07-15 DE DE19681768934 patent/DE1768934A1/de active Pending
-
1969
- 1969-06-24 CH CH964469A patent/CH512522A/de not_active IP Right Cessation
- 1969-06-30 CA CA055716A patent/CA923834A/en not_active Expired
- 1969-06-30 IL IL6932511A patent/IL32511A/xx unknown
- 1969-07-02 GB GB3335669A patent/GB1228698A/en not_active Expired
- 1969-07-04 AT AT643769A patent/AT287188B/de active
- 1969-07-04 IE IE918/69A patent/IE33418B1/xx unknown
- 1969-07-05 RO RO6900060439A patent/RO61383A/ro unknown
- 1969-07-08 LU LU59055A patent/LU59055A1/xx unknown
- 1969-07-14 NO NO2941/69A patent/NO124538B/no unknown
- 1969-07-14 SE SE6909961A patent/SE372276B/xx unknown
- 1969-07-14 PL PL1969134799A patent/PL82620B1/pl unknown
- 1969-07-14 DK DK380869AA patent/DK122433B/da unknown
- 1969-07-14 CS CS4976A patent/CS150676B2/cs unknown
- 1969-07-14 BE BE736069A patent/BE736069A/xx unknown
- 1969-07-14 JP JP44055134A patent/JPS5212242B1/ja active Pending
- 1969-07-14 ES ES369475A patent/ES369475A1/es not_active Expired
- 1969-07-15 FI FI692106A patent/FI47375C/fi active
- 1969-07-15 NL NL6910881A patent/NL6910881A/xx unknown
- 1969-07-15 FR FR6924062A patent/FR2014613A1/fr not_active Withdrawn
- 1969-07-15 EG EG381/69*A patent/EG10148A/xx active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3196315A1 (en) | 2016-01-19 | 2017-07-26 | ORITEST spol. s r.o. | Spherical pellets, manufacturing process of such pellets, use, and a detection tube comprising such pellets |
Also Published As
| Publication number | Publication date |
|---|---|
| PL82620B1 (enrdf_load_stackoverflow) | 1975-10-31 |
| IL32511A0 (en) | 1969-11-12 |
| IE33418L (en) | 1970-01-15 |
| GB1228698A (enrdf_load_stackoverflow) | 1971-04-15 |
| EG10148A (en) | 1976-11-30 |
| NO124538B (enrdf_load_stackoverflow) | 1972-05-02 |
| CS150676B2 (enrdf_load_stackoverflow) | 1973-09-04 |
| IE33418B1 (en) | 1974-06-26 |
| BE736069A (enrdf_load_stackoverflow) | 1970-01-14 |
| FR2014613A1 (enrdf_load_stackoverflow) | 1970-04-17 |
| AT287188B (de) | 1971-01-11 |
| NL6910881A (enrdf_load_stackoverflow) | 1970-01-19 |
| CA923834A (en) | 1973-04-03 |
| CH512522A (de) | 1971-09-15 |
| ES369475A1 (es) | 1971-06-01 |
| DK122433B (da) | 1972-03-06 |
| SE372276B (enrdf_load_stackoverflow) | 1974-12-16 |
| FI47375C (fi) | 1973-11-12 |
| LU59055A1 (enrdf_load_stackoverflow) | 1969-11-18 |
| RO61383A (fr) | 1977-10-15 |
| IL32511A (en) | 1973-05-31 |
| JPS5212242B1 (enrdf_load_stackoverflow) | 1977-04-06 |
| FI47375B (enrdf_load_stackoverflow) | 1973-07-31 |
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