IE33418L - Biochemical enrichment process - Google Patents

Biochemical enrichment process

Info

Publication number
IE33418L
IE33418L IE690918A IE91869A IE33418L IE 33418 L IE33418 L IE 33418L IE 690918 A IE690918 A IE 690918A IE 91869 A IE91869 A IE 91869A IE 33418 L IE33418 L IE 33418L
Authority
IE
Ireland
Prior art keywords
substance
complex
amino
inhibitor
addition product
Prior art date
Application number
IE690918A
Other versions
IE33418B1 (en
Inventor
H Fritz
E Werle
Original Assignee
Bayer Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Ag filed Critical Bayer Ag
Publication of IE33418L publication Critical patent/IE33418L/en
Publication of IE33418B1 publication Critical patent/IE33418B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6445—Kallikreins (3.4.21.34; 3.4.21.35)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

1,228,698. Enzymes; enzyme inhibitors. FARBENFABRIKEN BAYER A.G. 2 July, 1969 [15 July, 1968], No. 33356/69. Heading C3H. A process for the enrichment of a substance capable of forming a complex with a polypeptide which comprises linking a polypeptide by a covalent linkage to a carrier to form an addition product, introducing basic groups into the addition product, contacting the thus modified addition product in water with a solution of the substance to be enriched whereby a complex between the said substance and the addition product is formed, purifying the complex by washing, causing the complex to dissociate, and isolating the said substance. The substance to be purified may be an enzyme e.g. trypsin, chymotrypsin, kallicrein, plasmin, pepsin, rennin, ribonuclease, thrombin, amylase, papain, hyaluronidase, carboxypeptidase A or B, pancreatopeptidase E, penicillinase or cholinesterase, or an inhibitor such as kallicreintrypsin inhibitor ("Kuritz inhibitor") or the trypsin inhibitors obtained from pig and bovine pancreas, from seminal vesicles, from plant seeds or from potatoes. When the substance is an enzyme the polypeptide used in the process may be the corresponding inhibitor and vice versa. The carrier may be a resin containing anhydride or acid chloride groups or activated fluorine atoms, or carboxyl groups plus activated groups. The necessary basic groups may be introduced by treatment with N,N - dimethylethylene - diamine, agmatine, N - methyl- N<SP>1</SP> - (3 - amino - propyl) - piperazine, 4- amino - pyridine, 2 - amino - pyridine, or 3- amino 1 - cyclohexyl - aminopropane. Purification of the complex may be by washing with water and buffer solutions, and dissociation may be by altering pH or ion concentration, by displacement, or by adding urea or guanidine salts. [GB1228698A]
IE918/69A 1968-07-15 1969-07-04 Biochemical enrichment process IE33418B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19681768934 DE1768934A1 (en) 1968-07-15 1968-07-15 Process for the enrichment of polypeptides, preferably of enzymes and enzyme inhibitors

Publications (2)

Publication Number Publication Date
IE33418L true IE33418L (en) 1970-01-15
IE33418B1 IE33418B1 (en) 1974-06-26

Family

ID=5699923

Family Applications (1)

Application Number Title Priority Date Filing Date
IE918/69A IE33418B1 (en) 1968-07-15 1969-07-04 Biochemical enrichment process

Country Status (21)

Country Link
JP (1) JPS5212242B1 (en)
AT (1) AT287188B (en)
BE (1) BE736069A (en)
CA (1) CA923834A (en)
CH (1) CH512522A (en)
CS (1) CS150676B2 (en)
DE (1) DE1768934A1 (en)
DK (1) DK122433B (en)
EG (1) EG10148A (en)
ES (1) ES369475A1 (en)
FI (1) FI47375C (en)
FR (1) FR2014613A1 (en)
GB (1) GB1228698A (en)
IE (1) IE33418B1 (en)
IL (1) IL32511A (en)
LU (1) LU59055A1 (en)
NL (1) NL6910881A (en)
NO (1) NO124538B (en)
PL (1) PL82620B1 (en)
RO (1) RO61383A (en)
SE (1) SE372276B (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4054646A (en) 1973-07-30 1977-10-18 General Electric Method and apparatus for detection of antibodies and antigens
FR2802817B1 (en) * 1999-12-23 2002-10-11 Centre Nat Rech Scient NOVEL GLYCOSIDASE INHIBITORS AND THEIR PHARMACOLOGICAL APPLICATIONS, PARTICULARLY FOR TREATING DIABETES
CN101489655A (en) 2006-07-14 2009-07-22 威斯康星旧生研究基金会 Adsorptive membranes for trapping viruses
PL3196315T3 (en) 2016-01-19 2020-07-13 Oritest Spol. S R.O. Spherical pellets, manufacturing process of such pellets, use, and a detection tube comprising such pellets
CN117099869B (en) * 2023-06-27 2024-11-15 河南工业大学 A preparation method of wheat protein peptide chelated calcium

Also Published As

Publication number Publication date
NL6910881A (en) 1970-01-19
IL32511A0 (en) 1969-11-12
FR2014613A1 (en) 1970-04-17
CS150676B2 (en) 1973-09-04
IE33418B1 (en) 1974-06-26
AT287188B (en) 1971-01-11
BE736069A (en) 1970-01-14
DE1768934A1 (en) 1972-03-16
FI47375C (en) 1973-11-12
JPS5212242B1 (en) 1977-04-06
EG10148A (en) 1976-11-30
GB1228698A (en) 1971-04-15
CA923834A (en) 1973-04-03
SE372276B (en) 1974-12-16
CH512522A (en) 1971-09-15
LU59055A1 (en) 1969-11-18
IL32511A (en) 1973-05-31
PL82620B1 (en) 1975-10-31
FI47375B (en) 1973-07-31
ES369475A1 (en) 1971-06-01
RO61383A (en) 1977-10-15
DK122433B (en) 1972-03-06
NO124538B (en) 1972-05-02

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